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Citations for Beckman :
101 - 150 of 161 citations for Corning Hygromycin B Solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... Cells were resuspended in p24 primary antibody solution (1:100 dilution of p24-FITC antibody clone KC57 (Beckman Coulter) diluted in perm/wash buffer ...
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bioRxiv - Cell Biology 2020Quote: ... and 5 % solutions on top of each other in a 13.2 ml open-top polyallomer tube (331372, Beckman Coulter). The 1 ml concentrates were overlaid on top of the gradient ...
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bioRxiv - Microbiology 2021Quote: ... Linear sucrose gradients were prepared by layering 1.8 ml each of sucrose solutions (wt/wt; prepared in 5 mM EDTA, pH 7.5) into 14-by 95-mm ultracentrifuge tubes (Beckman) in the following order ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA clean-up was performed by incubating RNA solution in 2 volumes of AMPure XP Beads (Beckman Coulter, A63881) for 2 minutes at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... myelin was layered onto 3 mL ice cold 0.85 M sucrose solution in a 10 mL ultracentrifuge tube (Beckman) before centrifugation at 75 000 x g for 30 min at 4 °C on a Beckman Ultracentrifuge with MLA-55 rotor (acceleration 7 ...
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bioRxiv - Microbiology 2024Quote: ... Approximately 5.1 ml of the solution was transferred to an ultracentrifuge tube (Beckman Coulter QuickSeal, 13 mm × 51 mm) and heat-sealed ...
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bioRxiv - Microbiology 2023Quote: ... 0.5 mL of 30% and 1.8 mL 10% of iodixanol solutions and floated into the gradient by ultracentrifugation in a SW60Ti rotor (Beckman) at 192,000 x g ...
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bioRxiv - Cell Biology 2022Quote: ... 350 μl of the supernatant was loaded on top of a 1.8 ml step gradient composed of 10 and 18% OptiPrep in Ringer’s solution and centrifuged for 30 min at 20,000 rpm in a swing bucket SW-55 rotor (Beckman Coulter) at 4°C ...
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bioRxiv - Plant Biology 2022Quote: ... was gently layered on top of 4.4 mL sucrose gradient solution (15 to 55%) in a 5.1 mL Quick-Seal polypropylene tube (Beckman Coulter) and centrifuged at 45,000 rpm for 65 min at 4°C (Sorval NVT 90 ...
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bioRxiv - Immunology 2023Quote: ... Layers of 2.1 mL of each solution were successively poured into 12.5 mL polyallomer tubes (Beckman-Coulter, Cat. # 331372), starting from the most concentrated solution (50 % ...
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bioRxiv - Immunology 2024Quote: ... The resulting pooled solution was then centrifuged at 40,000 rpm (20,400 x g) for 120 minutes (50Ti rotor in Beckman L350) to pellet the LPS fraction.
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bioRxiv - Immunology 2024Quote: ... This fixation solution was washed away and cells were stained intracellularly with anti-HIV-Gag-p24 (KC57, Beckman Coulter), anti-puromycin (2A4 ...
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bioRxiv - Synthetic Biology 2024Quote: ... A 2 µg/µL BSA solution (in water) was transferred from a 384-well polypropylene 2.0 Plus Source microplate (Beckman) using the 384PP_Plus_BP fluid type into a 384-well destination plate (BioRad ...
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bioRxiv - Molecular Biology 2020Quote: ... The gradient was formed by adding 3 mL of the iodixanol solutions to a 17 mL polypropylene tube (Beckman Coulter). Ultracentrifugation was performed using a SW Type 32.1 Ti rotor in Optima XPN-100 Ultracentrifuge (Beckman Coulter ...
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bioRxiv - Bioengineering 2020Quote: ... the EV pellets were resuspended in 3 ml 0.32 M sucrose (diluted in PBS) solution and centrifuged again at 100,000xg for 60 minutes at 4°C (SW60 rotor, Beckman Coulter). Sucrose supernatant was removed and pellet was resuspended in 500 μl phosphate-buffered saline containing 0.01% bovine serum albumin (0.2 μm filtered ...
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bioRxiv - Cell Biology 2020Quote: 5-20% sucrose gradients were prepared on a Biocomp Gradient Station IP from 5% and 20% solutions of sucrose in gradient buffer (50mM Tris HCl pH7.5, 150mM NaCl, 1mM EDTA) to Seton tubes (Beckman Coulter). Single cell suspensions of approximately 3×108 cells from thymus and bone marrow were obtained as above ...
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bioRxiv - Microbiology 2020Quote: ... parkeri was overlaid on a 30% MD-76R (Merry X-Ray) gradient solution in ultracentrifuge tubes (Beckman/Coulter Cat 344058). Gradients were centrifuged at 18,000 r.p.m ...
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bioRxiv - Genomics 2020Quote: ... the beads were removed from the solution using a magnet and DNA was purified using 1.8× volume of Agencourt AMPure XP (Beckman Coulter) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: LCM samples were washed from lysis buffer by mixing with an equal portion of SPRI bead solution (Beckman Coulter, A63881), incubated 5 min R/T and washed twice with 100% EtOH under magnetic field ...
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bioRxiv - Plant Biology 2023Quote: ... and all granules purified within this fraction was resuspended in known volumes of Isoton II electrolyte solution (Beckman Coulter, Indianapolis). The suspension was analysed in a Multisizer 4e Coulter counter (Beckman Coulter ...
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bioRxiv - Neuroscience 2024Quote: ... the pellet was suspended in 250 μl of cold FACS buffer and the resulting solution was finally sorted on a MoFloAstrios device (Beckman) to reach a concentration of 410 cells/μl.
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bioRxiv - Developmental Biology 2023Quote: ... The top 1 mL was loaded onto a 52% isotonic Percoll solution and centrifuged at 27,000 × g for 10 min at 10°C (Optima-XPN-80, Beckman Coulter). Following centrifugation ...
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bioRxiv - Microbiology 2023Quote: ... and 5.5 ml was layered on top of a two-step sucrose gradient consisting of 5 ml of 40% sucrose solution layered on top of 1.5 ml of a 65% sucrose solution in a 14 x 89mm Ultra-Clear tube (Beckman Coulter). All sucrose (w/w ...
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bioRxiv - Microbiology 2023Quote: ... Cells were washed twice with 200µl BD 1X Perm/Wash solution and stained 1h at 4°C with anti-HIV-1 p24 (RD1/PE, Beckman Coulter #6604667 ...
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bioRxiv - Molecular Biology 2023Quote: ... The lysates were sheared with a 25-gauge 5/8 needle and loaded onto CsCl solution (150% w/v) for ultracentrifugation in NVT 65.2 rotor (Beckman coulter) at 42,000 rpm for 20 hours at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... step gradients were made by superposing sucrose solutions of different concentration (50%, 40%, 30%, 20%, 10%) in an ultra-clear polycarbonate tube (Beckman), and a linear gradient was allowed to form overnight at 4 °C ...
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bioRxiv - Cell Biology 2022Quote: ... 1 mL samples were supplemented with 500 uL of Optiprep to yield a 20% iodixanol solution and underlayered beneath 1.5 mL layers of 9% and 12% Optiprep/PBS solutions in a 4.9 mL OptiSeal tube (Beckman-Coulter, 362185) using a disposable glass cannula ...
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bioRxiv - Biochemistry 2023Quote: ... The reaction mixture was applied onto the top of the gradient solution and was centrifuged at 41,000 rpm at 4 °C for 17 hrs using an SW41 Ti rotor (Beckman Coulter). The gradient was fractionated using a Piston Gradient Fractionator (BioComp) ...
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bioRxiv - Microbiology 2023Quote: ... Carbon source stock solutions were added to microplates using a Biomek FxP liquid handling robot (Beckman Coulter, Indianapolis, IN, USA) and kept in an anaerobic chamber (Coy ...
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bioRxiv - Biochemistry 2024Quote: ... and the solution was centrifuged for 45 min at 20,000g at 1 °C (Beckman Coulter J2-21, JA-20 rotor). The supernatant was then loaded onto a 5 ml HisTrap column (Cytiva ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and fragments between 400-500 bp were selected for purification using a 25X AMPure beads solution (Beckman Coulter Inc, Indianapolis, IN). Fragments were then amplified with a NEBNext Ultra Q5 PCR protocol ...
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bioRxiv - Systems Biology 2020Quote: ... and 1 mL of 5% (w/v) iodixanol solutions on top of each other in a 5 mL open top polyallomer tube (Beckman Coulter). Crude exosomes were resuspended in 200 µL PBS and overlaid on top of the gradient ...
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bioRxiv - Genomics 2021Quote: ... Cells were washed with 25 mm supplemented HBSS before depleting the sample of unnucleated cells using IOTest 3 Lysing solution (Beckman Coulter) following provider instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Sample solution containing EVs was placed at the top of the tube (at RT) and ultracentrifugation (Beckman Optima L-90K Ultracentrifuge) was performed at 40,000 RPM for 16 hours at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... 750 µL of the 80% Histodenz solution were added and thoroughly mixed with 750µL of the protein-LUV sample in Thinwall centrifuge tubes (Beckman 50.2ti rotor). 1.5 mL of the 30% Histodenz solution was added as the intermediate layer ...
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bioRxiv - Cell Biology 2022Quote: ... which was then slowly poured on top of 10 ml cushion solution (2.05M sucrose with protease inhibitors, DTT and PMSF) in the ultracentrifugation tube (Beckman polyallomer #326823). After a 45-min spin at 24,600 rpm in pre-chilled SW32Ti rotor at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... the homogenized solution was added onto a sucrose cushion (2 ml) in a 10 ml Ultra-Clear centrifuge tube (Beckman Coulter). Separation of nuclei from the tissue was done by centrifugation (26.500xg at 4°C for 1.5 h ...
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bioRxiv - Molecular Biology 2020Quote: ... Samples were brought to a final volume of 300 μl if necessary with lysis buffer and loaded on a non-linear sucrose gradient prepared by layering different percentage of sucrose solutions (5%, 34% and 55%) into an Open-Top Thinwall Ultra-Clear Tube (Beckman, 344059). A 5x gradient buffer (100 mM HEPES pH 7.6 ...
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bioRxiv - Cancer Biology 2024Quote: ... Conventional flow cytometry analysis was performed on 200 µL of whole blood cells using a lyse no wash protocol (Versalyse lysing solution, Beckman Coulter) or on 2 ×106 PBMC labeled and analyzed using a Fortessa cytometer (BD Biosciences ...
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bioRxiv - Microbiology 2024Quote: ... cultures were diluted 1:20 in filtered saline solution (NaCl 0.9%) and fluorescent intensity was measured by flow cytometry using a CytoFLEX-S cytometer (Beckman Coulter, USA). The measurement of each biological replicate is the result of the mean of the fluorescence intensity of 30 000 events per sample ...
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bioRxiv - Cell Biology 2023Quote: ... protein-liposome or protein-LD mixtures were combined with equal volume of 60% Optiprep solution (D1556-250ML, sigma) and placed at the bottom of the centrifuge tube (Beckman, 344090), and 10% Opti-prep and 0% Optiprep solutions were carefully pipetted on the top ...
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bioRxiv - Cancer Biology 2022Quote: ... 1 mM DTT and 10 mM Tris-HCl in DEPC-treated water was added to the bottom of the lysis solution in ultracentrifuge tubes (Beckman Coulter) to form a gradient ...
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bioRxiv - Evolutionary Biology 2024Quote: ... to 1.5 mM with a 10-fold dilution and applied double-sided size selection with 90 µL of Agencourt AMPure XP solution (Beckman Coulter) at both removing long fragments and recovering short fragments ...
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bioRxiv - Neuroscience 2020Quote: ... Final 15 mL of solution was mixed by inverting the tube 10x and carefully pipetted into 2 ultracentrifuge tubes (Beckman Coulter #344059) onto the isolation buffer cushion (5 mL ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Clarified supernatants were layered over 2 ml of a 25% (w/v) sucrose solution in 13.2 ml Thinwall Polypropylene SW41 Ti ultracentrifuge tubes (Beckman-Coulter, Brea, USA) and made up to a total volume of 10 ml with PBS without calcium chloride or magnesium chloride (PBS[-]) ...
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bioRxiv - Genetics 2022Quote: ... The samples were size selected with 0.65x volume of Agencourt® AMPure® XP magnetic bead solution (Beckman Coulter, Brea, CA, USA), and suspended in 20µL elution buffer (Qiagen ...
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bioRxiv - Neuroscience 2023Quote: ... The nuclei suspension was layered onto 10 mL of 29% OptiPrep solution (Buffer + 50% OptiPrep) in a 38.5 mL Ultra-Clear tube (Beckman-Coulter, CA, USA) before being centrifuged at 7,333g for 30 minutes at 4°C ...
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bioRxiv - Plant Biology 2023Quote: ... The combined cAW and vAW solutions were then centrifuged using 29 x 104 mm (50 mL) polypropylene centrifuge tubes (Beckman Coulter, # 357007) at 10,000 x g for 1 h at 4 ⁰C (JA-17 rotor ...
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bioRxiv - Plant Biology 2023Quote: ... and 2 mL of 5% solution in a 14 x 89 mm (13.2 mL) thin walled ultra-clear centrifuge tubes (Beckman Coulter, Cat. # 344059). Approximately 250 µl of crude EV sample resuspended in 20 mM Tris-HCl pH 6.0 was slowly dispensed on top of the column and centrifuged at 100,000 x g for 17 h at 4 ⁰C (SW 41 Ti rotor ...
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bioRxiv - Cell Biology 2023Quote: ... saturated solution) to reach a final sucrose concentration of ∼40% and layered onto 62% sucrose in polycarbonate centrifuge tubes (Beckman Coulter, 34058). 35% (1.02M) ...