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Citations for Beckman :
101 - 150 of 1658 citations for Benzyl 2 3 4 5 6 d5 dimethyltetradecylammonium Bromide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... The supernatant was ultracentrifuged at 120,000 g for 3 h at 4°C using an MLS-50 rotor (Beckman Coulter, USA) to collect 120k pellet vesicles ...
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bioRxiv - Biophysics 2021Quote: The aggregation products formed in Eppendorf tubes under agitation conditions at pH 3 and pH 4 were centrifuged using an Optima MAX-XP ultracentrifuge (Beckman Coulter) in a TLA-55 rotor at 40,000 rpm at 20°C for 45 min ...
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bioRxiv - Microbiology 2024Quote: ... and resolved by ultracentrifugation at 36,000 rpm for 3 h at 4°C with the fastest braking in Optima XPN-80 Ultracentrifuge equipped with SW-41Ti rotor (Beckman Coulter). Gradients were fractionated (0.5 mL/fraction ...
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bioRxiv - Biophysics 2024Quote: ... Each seed mix was clarified using an ultra-centrifuge (Beckman Optima MAX-XP, 90,000 RPM, 4°C, 5 min) then snap-frozen in small aliquots and stored at -80°C ...
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bioRxiv - Microbiology 2022Quote: ... Fractions were diluted in 5 ml PBS and washed by ultracentrifugation at 200,000 × g (60 min, 4°C, AH-650 rotor, 5 ml Beckman Ultra-Clear tubes ...
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bioRxiv - Microbiology 2024Quote: ... Samples within each biological replicate were centrifuged simultaneously for 2.5 h at 209,490 x g and 4°C using the SW41Ti rotor in an LE-80 ultracentrifuge (Beckman). Subsequently ...
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bioRxiv - Molecular Biology 2024Quote: ... Proliferation was measured after 4-5 days either by trypan-blue mediated count (Vi-cell blu counter, Beckman Coulter), or by adding the Prestoblue dye (Life Technologies ...
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bioRxiv - Molecular Biology 2021Quote: ... Gradients were centrifuged at 36000 RPM for 2 hours at 4°C in a SW41 rotor (Beckman Coulter) and then fractions were collected using a gradient collector (Teledyne ISCO gradient station ...
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bioRxiv - Microbiology 2020Quote: ... Purification was done by ultracentrifugation for 2 h at 154,000xg at 4°C in a SW41 rotor (Beckman) using a two-step sucrose gradient (20%/50% w/v in HNE) ...
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bioRxiv - Cell Biology 2020Quote: ... the cleared supernatant was ultracentrifuged at 47,000 g for 2 hours at 4 °C using the JA25.50 rotor (Beckman). The virus was resuspended overnight in 180 μL D10 medium at 4 °C ...
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bioRxiv - Cell Biology 2021Quote: ... 4 °C for 2 h in an Optima L-100XP ultracentrifuge (with SW 55 Ti rotor, Beckman Coulter). Fractionation was done manually by collecting every 200 μl volume from the top till the bottom of each centrifuge tube ...
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bioRxiv - Molecular Biology 2023Quote: ... Digested extracts were then loaded on 1 M sucrose cushions 116 and centrifuged at 70,000 rpm for 2 hours at 4°C in a TLA100.4 rotor (Beckman). Ribosome pellets were resuspended in 300 µl of TRIzol reagent (Invitrogen) ...
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bioRxiv - Biophysics 2024Quote: ... The virus was pelleted from supernatants via centrifugation at 14000 rpm for 6 hours at 4°C using a Beckman J2-21 high-speed ultracentrifuge fitted with a JA14 rotor (Beckman Coulter Inc., Fullerton, CA, USA). After carefully removing supernatants ...
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bioRxiv - Plant Biology 2020Quote: MP of pollen protoplasts was assessed by staining with 1 µM DiBAC4(3) for 5 min (Breygina et al. 2010) on Gallios flow cytometer (Beckman Coulter), analysis was performed with FlowJo software (TreeStar) ...
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bioRxiv - Molecular Biology 2024Quote: ... bottom layer was made by diluting OptiPrep™ solution with clarified HM or digesta sample (2.25 mL OptiPrep™ + 0.75 mL sample = 3 mL) into a 5 mL open-top thin wall ultra-clear tube (Beckman Coulter). A discontinuous gradient was made with 40% (w/v) ...
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bioRxiv - Pathology 2024Quote: ... with primers located in the U3 region of the 5’ and 3’ LTRs (Figure S1) and purified using “1X AmpureXP beads” (Beckman Coulter). ONT (Oxford nanopore technologies ...
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bioRxiv - Plant Biology 2021Quote: ... sucrose was transferred to the bottom of the tube using a Pasteur pipette and centrifuged at 27,000 rpm for 3 hours at 4° C using an SW28 Ti rotor (Beckman Coulter, Brea, CA). Each pellet containing the virus particles was resuspended in 1 mL resuspension buffer overnight with gentle shaking at 4 °C ...
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bioRxiv - Cell Biology 2022Quote: ... and centrifuged at 16000 ×g for 3 hours at 4 °C in an Avanti J-25 centrifuge (Beckman Coulter Life Sciences, USA) with a JA-25.50 fixed-angle aluminum rotor (Beckman Coulter Life Sciences ...
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bioRxiv - Molecular Biology 2021Quote: ... sucrose gradients prepared with lysis buffer without Triton X100 and centrifuged at 32,000 rpm for 3 hrs at 4 °C in an SW41Ti rotor (Beckman Coulter, Cat:331362). The sucrose gradients were fractionated using BR-188 Density Gradient Fractionation System with 60% (m/v ...
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bioRxiv - Microbiology 2020Quote: MT-2 cells were collected and washed three times by pelleting at 200 x g for 5 min at 4°C in an Allegra centrifuge (Beckman) and resuspending cells in ice-cold PBS ...
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bioRxiv - Cell Biology 2022Quote: ... brought up to 3 ml using sterile 20 mM Tris-HCl pH 7.5 and pelleted at 40,000g and 4°C for 60 minutes (TLA100.3 rotor, Optima Max-XP ultracentrifuge, Beckman Coulter, Inc.). Finally ...
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bioRxiv - Molecular Biology 2020Quote: ... The following day the viral pellet was resuspended in 5 ml PBS and centrifuged at 20,000 rpm for 90 mins at 4°C (Optima XL-100K, Beckman Coulter). The pellet was then resuspended in the required volume of PBS before being aliquoted and stored at −70°C.
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bioRxiv - Immunology 2021Quote: ... 1×106 thawed PBMCs were centrifuged (750×g for 5 minutes at 4°C) using a swinging bucket rotor (Beckman Coulter Avanti J-15RIVD with JS4.750 swinging bucket ...
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bioRxiv - Genomics 2023Quote: ... The suspension was incubated on ice for 5 min and centrifuged at 20,000 ×g (10 min, 4 °C, JA-20 rotor, Avanti J-26 XP, Beckman Coulter). The pellet was re-centrifuged for 5 min and both supernatants were combined into a new tube ...
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bioRxiv - Neuroscience 2022Quote: ... trypsin-dissociated cells on 96-well plates were centrifuged at 1,000g for 5 min at 4°C using a swinging-bucket rotor for microplates (Beckman S6096) in an Allegra X-30R centrifuge (Beckman) ...
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bioRxiv - Cell Biology 2024Quote: ... The step gradients were centrifuged for 5 h at 132.000 x g at 4 °C (SW60 Beckman and Coulter, # 335649). Subsequently ...
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bioRxiv - Cell Biology 2020Quote: ... Lysates were loaded on the top of the gradient and centrifuged at 260,000 × g and 4 °C for 2 h using SW 41 rotor (Beckman). Collection of polysomal fractions were done using Piston Gradient Fractionator (BioComp) ...
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Human Sex Matters: Y-linked lysine demethylase 5D drives accelerated male osteogenic differentiationbioRxiv - Cell Biology 2021Quote: ... Virus was transferred into conical tubes and spin at 20000rpm for 2 hours at 4°C in (Beckman TST2838) swinging bucket rotor ...
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bioRxiv - Biochemistry 2021Quote: ... 2 mM DTT) by being centrifuged at 73000 rpm for 45 minutes at 4°C (Beckman Coulter TL120.2 rotor). The mitoribosomal pellet was subsequently resuspended in ribosome resuspension buffer (25 mM HEPES-KOH pH = 7.5 ...
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bioRxiv - Neuroscience 2022Quote: ... and centrifuged at 24,000 rpm for 2 h at 4°C using a SW-Ti32 swinging bucket (Beckman Coulter). The upper layer was discarded ...
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bioRxiv - Cell Biology 2021Quote: ... Pellets were resuspended in 0.9% NaCl and spun at 110,000 × g for 2 hours at 4°C in an XPN-80 ultracentrifuge equipped with a Type 50.4 Ti rotor (Beckman Coulter ...
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bioRxiv - Microbiology 2021Quote: ... The VLPs were concentrated by ultracentrifugation for 2 h at 31,000 rpm at 4 °C (Beckman SW-31Ti rotor), and then resuspended in 500 μL of Ex-Cell 405 medium ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were then centrifuged at 82,600×g at 4°C for 2 h (Beckman ultracentrifuge optima L-100 XP). After the first ultracentrifugation ...
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bioRxiv - Plant Biology 2023Quote: ... The supernatant from this step was decanted into the new tubes and centrifuged at 39,800 x g for 2 h at 4 ⁰C (JA-17 rotor, Beckman Coulter ...
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bioRxiv - Molecular Biology 2023Quote: ... 1-1.5 μg of total protein was loaded onto a 10% to 50% sucrose gradient and centrifuged at 36,000 rpm for 2 hours at 4°C using an SW41 rotor (Beckman). Polysome profiles were visualized ...
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bioRxiv - Cancer Biology 2024Quote: ... 100 μg/mL cycloheximide) for 2 h at 40,000 g at 4 °C using an SW41 rotor (Beckman Coulter). Gradients were fractionated using Teledyne Isco Tris Peristaltic Pump and fractions were collected and pooled according to the UV trace ...
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bioRxiv - Cell Biology 2024Quote: ... samples were ultracentrifuged at 160,000 xg for 2 h at 4 °C in ultracentrifuge tubes (Beckman Coulter Cat #328874). The resulting pellet contained the insoluble AMB-1 cell fraction and the supernatant contained the soluble fraction ...
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bioRxiv - Cell Biology 2024Quote: ... The supernatant was then subjected to ultracentrifugation at 100,000 x g for 2 h at 4 °C to sediment small EVs (Optima L-80K ultracentrifuge, Beckman Coulter ...
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bioRxiv - Genetics 2023Quote: ... Fifteen A260 units of WCEs were layered on a pre-chilled 10-50% sucrose gradient and centrifuged at 40,000 rpm for 2 h at 4°C in a SW41Ti rotor (Beckman). Gradient fractions were continuously scanned at A260 using the BioComp Gradient Station and polysome to monosome (P/M ...
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bioRxiv - Cancer Biology 2023Quote: ... the obtained supernatant was filtered through a 0.22 μm filter before ultracentrifugation at 150,000 g for 2 h at 4 °C (Beckman Coulter). The nsEV-containing pellet was resuspended in phosphate buffer saline (PBS).
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bioRxiv - Molecular Biology 2023Quote: ... The gradients were centrifuged at 38,300 rpm for 2 hours and 45 min at 4 °C in a SW-40Ti (Beckman) rotor using an Optima XE-100 Ultracentrifuge (Beckman) ...
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bioRxiv - Molecular Biology 2023Quote: ... The gradients were centrifuged at 38,300 rpm for 2 hours and 45 min at 4 °C in a SW-40Ti (Beckman) rotor using an Optima XE-100 Ultracentrifuge (Beckman) ...
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bioRxiv - Plant Biology 2023Quote: ... Extract was then centrifuged at 40 000 rpm for 2 hours at 4°C using the 50Ti rotor (Beckman). A protocole previsouly used in the laboratory and described in Maclot et al ...
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bioRxiv - Molecular Biology 2024Quote: ... were layered on top of each sucrose gradient and centrifuged (41,000 rpm for 2 hours at 4°C) in an SW41 Ti rotor (Beckman). UV profiling (254 nm ...
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bioRxiv - Microbiology 2024Quote: The virus solutions were centrifuged at 106800 × g for 3 hours at 4 °C using a SW32Ti rotor (Beckman Coulter, Fullerton, CA, USA). The virus pellets were suspended in phosphate-buffered saline (PBS) ...
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bioRxiv - Cancer Biology 2022Quote: ... 10 nM leptomycin b, vehicle control or library compounds (Supplementary Tables 2, 3) were added using an Echo liquid handler (Labcyte/Beckman Coulter). Cells were incubated for a further 24 h then fixed in 4% paraformaldehyde and stained with DAPI and Alexa Fluor 488 Phalloidin (ThermoFisher) ...
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bioRxiv - Genetics 2020Quote: ... Timepoints were measured every 2-3 days by flow cytometry analysis of >7,000 cells (either a BD Accuri C6 or Beckman Coulter CytoFLEX). Data was analyzed using Cytoflow (https://github.com/bpteague/cytoflow ...
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bioRxiv - Genomics 2021Quote: ... samples were purified with 45 µl of Agencourt AMPure XP magnetic beads with a sample to beads ratio of 3:2 (Beckman Coulter), normalized by Qubit quantification and pooled to generate a 4 nM library ...
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bioRxiv - Biophysics 2021Quote: ... and the fractions containing the protein of interest were combined and concentrated to 2mL at a final concentration of 3.85 mg/mL by repeated centrifugation in an Amicon Ultra Centrifugal Filter at 4k rpm for 5 min at 4 °C (Beckman SX4250). The concentrated protein was incubated overnight with TEV (1:50 mass ratio ...
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bioRxiv - Biochemistry 2020Quote: ... cells were centrifuged at 4 ℃ for 5 minutes at 300g and suspended in HBSS and analyzed with a flow cytometer (CytoFLEX, Beckman Coulter). Mean fluorescence intensity was calculated for each group.