Labshake search
Citations for Beckman :
101 - 150 of 250 citations for BD 3 Mouse since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... containing the phosphatases and proteases inhibitors and centrifuged at 100,000xg for 1 h through a 3 ml 1.4 M sucrose cushion using a TL100 centrifuge (Beckman). The crude heavy nuclear fraction and purified nuclei were resuspended in buffer A and extracted with 1% Triton X-100 for 5 min at 4°C (cNM ...
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bioRxiv - Microbiology 2019Quote: ... and adding this to 3 ml sterile inoculum water (Beckman Coulter, Inc. Brea, CA, cat no B1015-2) and 840 μl phenol red solution ...
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bioRxiv - Microbiology 2021Quote: ... The outer membrane vesicles (OMVs) were then isolated by ultracentrifugation (140 000xg, 4°C) for 3 hours (Beckman Coulter ...
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bioRxiv - Molecular Biology 2022Quote: ... Gradients were ultracentrifuged at 28.000g for 3 h at 4°C in a SW 28 Ti rotor (Beckman). 1.13 mL fractions were then collected using the Gradient Station model 153 ...
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bioRxiv - Biochemistry 2023Quote: ... Gradients were centrifuged at 35K rpm for 3 hrs at 4°C in a SW-41Ti rotor (Beckman) with maximum acceleration and no brake using a Beckman Optima L-90 Ultracentrifuge ...
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bioRxiv - Plant Biology 2023Quote: ... Cell density and mean cell volume were measured using a Coulter Counter (Multisizer 3, Beckman Counter, Brea, CA). For 35°C with photoautotrophic medium ...
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bioRxiv - Molecular Biology 2023Quote: ... 3 mM BME and centrifuged for 18 h at 30,000 rpm in a Ti 50.2 rotor (Beckman Coulter) at 4°C ...
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bioRxiv - Immunology 2024Quote: EVs isolated from cells were washed with ultracentrifugation for 3 hours with a SW41 Ti rotor (Beckman Coulter) at 100,000 x g at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... Four mL cultures at ∼10% parasitemia were harvested by centrifugation (2000 rpm for 3 min in a Beckman Coulter Allegra 25R table-top centrifuge ...
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bioRxiv - Bioengineering 2024Quote: ... Distribution of microbubble diameter and concentration was acquired with a Coulter counter (Multisizer 3; Beckman Coulter, Fullerton, California) prior to sonication ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were analysed for induction of apoptosis using a flow cytometer (BD FACSCanto™ II, Beckman Dickenson).
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bioRxiv - Cell Biology 2024Quote: ... HL-60 was fixed with BD fixation buffer before proceeding to Aurora (Cytek) or Cytoflex (Beckman Coulter) for analysis.
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bioRxiv - Microbiology 2019Quote: ... Conditioned media was then centrifuged at 36,000 × g for 3 hours at 4ºC (Type 45 Ti rotor, Beckman Coulter). OMV-containing pellets were resuspended in 2 mL ASW.
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bioRxiv - Microbiology 2019Quote: ... The entire 3 ml mixture was placed at the bottom of a Beckman SW41 ultracentrifuge tube (Beckman, Munich, Germany) and subsequently overlaid with 5 ml of ice-cold 30% Optiprep in TNE and 4 ml of ice-cold 5% Optiprep in TNE ...
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bioRxiv - Biophysics 2021Quote: ... The VP2 capsid protein fractions were pooled and subjected to centrifugation at 28,000 rpm (139,065 × g) for 3 h at 4°C (Sw40Ti rotor, Beckman Coulter). The pellet was resuspended in 50 mM Tris (pH 7.4) ...
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bioRxiv - Cell Biology 2021Quote: ... and centrifuged at 97,658 x g for 3 h at 4°C using a SW41Ti rotor (Beckman & Coulter, # 331362). The polysome fractions were collected using the Foxy R1 Fraction Collector and immediately snap frozen with liquid nitrogen and stored at −80 °C ...
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bioRxiv - Cell Biology 2021Quote: ... and centrifuged at 40,000 rpm (∼128,000 × g) for 3 h at 4°C using an MLS-5 rotor (Beckman Coulter). Aldolase (158 kDa ...
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bioRxiv - Plant Biology 2020Quote: ... 50 μg/ml cycloheximide) and centrifuged for 3 h at 55,000 g and 4 °C in a Type 70 Ti rotor (Beckman). The pellet was dissolved in 1% SDS ...
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bioRxiv - Molecular Biology 2022Quote: ... Gradients were spun for 3 hours at 4° C and 36,000 RPM (221,632 x g) on SW41-Ti rotor (Beckman Coulter). Monosome fractions were isolated from each sucrose gradient with Brandel Density Gradient Fractionator using 1.5 mL/min flow speed and 60% CsCl ...
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bioRxiv - Neuroscience 2022Quote: ... The samples were separated through ultracentrifugation for 1 hr at 200,000 g (spin 3 at 49,000 r.p.m. in Beckman TLS-55 rotor). Top layers of supernatants were removed ...
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bioRxiv - Microbiology 2022Quote: ... phage suspension was layered on top of a 5–20 % sucrose (w/vol, in Tris-HCl pH 7.2 with 3 mM MgCl2 and 10 mM CaCl2) and centrifuged (Beckman Optima L-K90 ...
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bioRxiv - Microbiology 2022Quote: ... The light-scattering zone was collected and further purified in 20– 70 % sucrose gradient (w/vol, in Tris-HCl pH 7.2 with 3 mM MgCl2 and 10 mM CaCl2) by centrifugation (Beckman Optima L-K90 ...
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bioRxiv - Immunology 2022Quote: ... All experiments in this study were performed using a 3-laser CytoFLEX Flow Cytometer (Beckman Coulter, Brea, CA, USA) and operated using the CytExpert Software v2.4.0.28 (Beckman Coulter) ...
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bioRxiv - Cell Biology 2020Quote: ... The harvested sEVs were resuspended with 3 mL double filtered PBS and centrifuged with MLA-55 rotor (Beckman Coulter) under 100,000 × g at 4 °C for 70 mins to remove non-EV contaminants ...
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bioRxiv - Microbiology 2019Quote: ... Gradients were centrifuged at 210,000 x g during 3 h at 4°C using a SW-41 swinging bucket rotor (Beckman) in an Optima L-100 XP Ultracentrifuge (Beckman Coulter ...
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bioRxiv - Genetics 2019Quote: ... The relative growth rate was determined by counting cells over the 3 day period using Coulter Counter Z2 (Beckman).
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bioRxiv - Biochemistry 2022Quote: ... The membrane was solubilized for 3 hours at 4°C before separation by ultracentrifugation at 100,000 g (Ti45, Beckman) for 45 min ...
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bioRxiv - Neuroscience 2022Quote: ... supernatant was centrifuged for 3 h at 115,000 g and 4°C using the XPN-80 ultracentrifuge equipped with a Ti70 rotor (Beckman). EV pellets were resolved in 1 mL ice-cold PBS (Gibco ...
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bioRxiv - Neuroscience 2022Quote: ... myelin was layered onto 3 mL ice cold 0.85 M sucrose solution in a 10 mL ultracentrifuge tube (Beckman) before centrifugation at 75 000 x g for 30 min at 4 °C on a Beckman Ultracentrifuge with MLA-55 rotor (acceleration 7 ...
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bioRxiv - Microbiology 2023Quote: ... The tube was centrifuged for 3 hours at 150’000 x g (4°C) using a SW 32.1 Ti swinging bucket (369651, Beckman Coulter). Finally ...
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bioRxiv - Cell Biology 2023Quote: ... 0.01% Sodium Azide in PBS and measured by recording 3 × 104 events using CytoFLEX and Gallios cytometers (Beckman Coulter). The resulting data were analysed using FlowJo v10 (TreeStar) ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were treated with 20nM of gemcitabine and cells were counted 3 days later using the Z1 Coulter (Beckman).
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bioRxiv - Bioengineering 2023Quote: ... MB concentration and number- and volume-weighted size distributions were measured using a Multisizer 3 Coulter Counter (Beckman Coulter). MB concentration (𝑐i ...
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bioRxiv - Neuroscience 2023Quote: ... the liposomes were isolated by centrifugation for 3 h 40 mins at 55,000 rpm at 4°C in a SW 60 rotor (SW 60 Ti, Beckman). 20 µl were harvested from the top of the gradient and mixed with 8 µl of 4x Laemmli buffer (final 62.5 mM Tris-HCl ...
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bioRxiv - Plant Biology 2024Quote: ... and centrifuged for 3 h at 260,000 g using an SW 55 Ti swinging-bucket rotor (Beckman Coulter, USA). The final supernatant was discarded ...
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bioRxiv - Microbiology 2023Quote: ... and RNA were purified using 3 volume of absolute isopropanol and 1.8 volume of Agencourt RNAClean XP beads (Beckman). RNA was eluted with 6 µL of water ...
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bioRxiv - Microbiology 2020Quote: ... and overlaid with 6 ml of 30% and 3 ml of 5% sucrose (w/v) followed by centrifugation at 32,000 rpm in SW41 rotor (Beckman Coulter) for 20 h ...
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bioRxiv - Molecular Biology 2020Quote: ... Debris was clarified by centrifuging at 18,000 ×g followed by fractionation on a discontinuous sucrose gradient (10-50 %) by ultracentrifugation at 39,000 rpm for 3 h in an SW41-Ti rotor (Beckman coulter). Monosome fractions were collected by pumping of Fluorinert FC-40 and using a fraction collector by measuring UV trace ...
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bioRxiv - Molecular Biology 2020Quote: ... The gradient was formed by adding 3 mL of the iodixanol solutions to a 17 mL polypropylene tube (Beckman Coulter). Ultracentrifugation was performed using a SW Type 32.1 Ti rotor in Optima XPN-100 Ultracentrifuge (Beckman Coulter ...
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bioRxiv - Genomics 2019Quote: ... Samples were size selected using a 0.5X Ampure XP cleanup to remove large fragments followed by 3 consecutive 1.5X Ampure XP (Beckman Coulter # A63880) bead cleanup followed by a final 1.2X Ampure XP cleanup to completely remove all residual primers and resuspended in a final volume of 20 µL EB using the manufacturer’s recommended protocol.
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bioRxiv - Cell Biology 2019Quote: ... Monosomes were isolated by sucrose gradients (10-50%) and centrifuged at 40,000 rpm for 3 hours in a SW41 rotor (Beckman Coulter). The extracted RNA was size-selected from 15% denaturing PAGE gels ...
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bioRxiv - Cancer Biology 2020Quote: The barcode split of the cDNA amplification reaction (from 10x Single Cell 3’ v3 Step 2.2) was purified via 1.2x SPRI Select (Beckman Coulter #B23317). cDNA products were eluted in 40 µL of EB ...
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bioRxiv - Neuroscience 2020Quote: ... reverse 5’-CTTA TTAGTCAGTCAGAAACAAC-3’).ThePCR amplicon was purified using RNAse-free SPRI beads (Agencourt RNAclean XP, Beckman-Coulter A63987) and then used for in vitro transcription with the HiScribe T7 ARCA mRNA Kit (with tailing ...
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bioRxiv - Microbiology 2021Quote: 3 mL of pseudoparticles were pelleted using a TLA-55 rotor with an Optima-MAX-E ultracentrifuge (Beckman Coulter, Inc.) for 2 hours at 42,000 rpm at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... Supernatants (3 ml) were loaded on the top of step gradients preformed in polyallomer tubes (14 by 89 mm, Beckman), containing 2 ml each of 1.82 ...
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bioRxiv - Molecular Biology 2020Quote: ... 1.3 M, 2.5 M) as indicated in the method (Vella et al., 2017) in ultra-clear SW40Ti tubes (Beckman Coulter). The sucrose gradients were centrifuged at 200,000 x g avg at 4°C for 173 min using a SW40Ti rotor (Beckman Coulter) ...
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bioRxiv - Microbiology 2020Quote: ... was overlaid on 25% sucrose in PBS and centrifuged at 26,000 rpm for 3 h at 4C using SW28 rotor (Beckman Coulter). Pellets were dissolved in PBS overnight at 4C and 1/3 of used for RNA was extraction using Macherey Nagel Virus RNA isolation kit without carrier RNA according to the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2020Quote: ... Samples are visualized on Cytoflex S, 3 lasers (488 nm, 405 nm and 638 nm) and 13 detectors (Beckman Coulter). Live cells were gated based on side scatter and forward scatter ...
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bioRxiv - Cell Biology 2021Quote: ... The post-nuclear supernatants were ultracentrifuged using SW41Ti rotor at 100,000 g for 3 h at 4 °C on Optima L-90K (Beckman Coulter). The fraction between 0.4–0.5 M sucrose was collected ...
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ACE2-lentiviral transduction enables mouse SARS-CoV-2 infection and mapping of receptor interactionsbioRxiv - Microbiology 2021Quote: ... UV-inactive SARS-CoV-2 was then partially-purified using a 20% sucrose cushion centrifuged at 175,000 x g for 3-4 hr using SW32Ti rotor (Beckman Coulter). The sample that has passed through the sucrose was collected and concentrated using Amicon Ultra-15 Centrifugal Filter Units with 100 kDa cutoff (Merck Millipore) ...