Labshake search
Citations for Beckman :
101 - 150 of 406 citations for Anti SWAP 70 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... followed by 12,000 rpm for 30 min using a fixed angle type 70 Ti rotor (Beckman Coulter, Brea, CA) and polycarbonate aluminum bottles with cap assembly (Beckman Coulter) ...
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bioRxiv - Cell Biology 2021Quote: ... The lysate was clarified by centrifuging at 183,960 x g for 88 min in Type 70 Ti rotor (Beckman). The clarified supernatant was incubated with 4 mL of IgG Sepharose 6 Fast Flow beads (GE Healthcare Life Sciences ...
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bioRxiv - Neuroscience 2022Quote: ... The tube was sealed and centrifuged at 58,400 RPM for 2.5 h using the 70 Ti fixed-angle rotor of an ultracentrifuge (Beckman Coulter). AAV was collected by extracting the 40%-60% iodixanol interface and washed using the Amicon centrifugal filter unit with 100 kDa cutoff (MilliporeSigma) ...
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bioRxiv - Neuroscience 2023Quote: ... DTT-treated EVs were pelleted at 100,000 x g for 70 min at 4°C in a TLA100 rotor (Beckman). The supernatant ...
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bioRxiv - Neuroscience 2023Quote: ... The supernatant was transferred to fresh tubes and centrifuged at 100,000 x g for 70 min at 4°C using a Ti45 rotor (Beckman). The 100,000 x g pellets were resuspended in 1 mL ice-cold PBS and brought to 60 mL using PBS ...
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bioRxiv - Biochemistry 2023Quote: ... The lysate was clarified via centrifugation (66,000 x g, 30 min, 4°C) in a Type 70 Ti rotor (Beckman). The supernatant was mixed with 1.5 mL of anti-FLAG M2 affinity gel (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2023Quote: ... and centrifuged them at 125,000 g (45,000 rpm) for 70 min (Optima Max TL with TLA-55 rotor, Beckman Coulter). The resultant sediment was dissolved in 40 µl of PBS and its protein concentration was estimated by a bicinchoninic acid (BCA ...
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bioRxiv - Neuroscience 2023Quote: ... Then AAVs were purified by ultracentrifugation (OptimaXE-90, Type 70 Ti rotor with 32.4mL OptiSealTM tubes, Beckman Coulter, USA). with 58400 rpm for 145 minutes in 18°C with Iodixanol density gradient solutions (15% ...
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bioRxiv - Neuroscience 2024Quote: ... Ultracentrifugation was performed for 2.5 h at 18 °C at 350,000 × g in a type 70 Ti rotor (Beckman Coulter). The interface between the 60 and 40% gradient was extracted along with the 40% layer ...
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bioRxiv - Microbiology 2023Quote: ... The lysate was clarified by centrifuging at 66,000 x g for 30 minutes in Type 70 Ti rotor (Beckman). The clarified supernatant was incubated with 2 ml of IgG Sepharose 6 Fast Flow beads (Cytiva ...
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bioRxiv - Immunology 2019Quote: The following antibodies were used: anti-p24-FITC (mouse monoclonal, Beckman and Coulter, #KC-57); anti-CD3-Pacific Blue (mouse monoclonal ...
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bioRxiv - Cell Biology 2019Quote: ... Intracellular proteins were then stained with anti-Gag KC57 RD1 antibody (1/100, Beckman Coulter) and/or anti-α-tubulin (5 μg/mL ...
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bioRxiv - Cell Biology 2023Quote: ... in the presence or absence of plate-bound anti-CD3 antibody (clone UCHT1, Beckman Coulter) and soluble anti-CD28 antibody (clone CD28.2 ...
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bioRxiv - Microbiology 2024Quote: ... cells were stained with primary anti-HIV-Gag antibody (Beckman-Coulter; KC57-RD1, mouse derived), and with α-mouse Alexa Fluor555-conjugated secondary antibody (Invitrogen ...
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bioRxiv - Microbiology 2024Quote: ... cells were stained with primary anti-HIV-Gag antibody (Beckman-Coulter; KC57-FITC, mouse derived), and cells were acquired using the MACs Quant Flow Cytometer (Milentyi ...
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bioRxiv - Biochemistry 2022Quote: ... 2 s off at 30 % amplitude for 10 min and cell debris was removed by centrifugation at 130,000 ×g for 45 min with a Type 70 Ti Rotor (Beckman Coulter). The supernatant was filtered through a 0.22 μm filter (Sigma ...
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bioRxiv - Biophysics 2019Quote: ... was transferred to a new polycarbonate ultracentrifuge tube and centrifuged at 100000 xg for 70 minutes at 4°C (Beckman Coulter Optima L-80 XP Ultracentrifuge ...
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bioRxiv - Cancer Biology 2019Quote: ... Exosomes were depleted from the complete media by ultracentrifugation for 70 minutes at 100,000x g using a Ultracentrifuge (Beckman Coulter) and SW28 swinging-bucket rotor ...
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bioRxiv - Cell Biology 2021Quote: ... The cell lysate was clarified via centrifugation at 66,000 x g for 30 min in a Type 70 Ti rotor (Beckman Coulter) at 4°C ...
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bioRxiv - Cancer Biology 2020Quote: Exosomes were depleted from the complete media by ultracentrifugation for 70 minutes at 100,000x g using an ultracentrifuge (Beckman Coulter) and SW28 swinging-bucket rotor ...
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bioRxiv - Microbiology 2020Quote: ... the sample was centrifuged for 16 h at 4°C and 100,000 rcf using a type 70 Ti rotor (Beckman Coulter). The pellet was gently washed with 500 μl of storage buffer (lysis buffer C + 10% (v/v ...
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bioRxiv - Cell Biology 2020Quote: ... The cell lysate was collected in a flask on ice and ultracentrifuged at 4°C for 25 min at 50000 rpm using a Ti-70 rotor (Beckman). The protein purification was done through affinity chromatography on a Ni-column (HiTrap chelating HP column 1 ml ...
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bioRxiv - Molecular Biology 2022Quote: ... The lysate was clarified at 503,000 rcf for 45 min at 4°C using a Type 70 Ti Rotor (Beckman Coulter). The supernatant was incubated with 3 mL IgG Sepharose 6 Fast Flow beads (Cytiva ...
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bioRxiv - Neuroscience 2020Quote: ... The V and VI fractions were diluted to a total volume of 12 mL with dfPBS and centrifuged at 140,000 × g for 70 min at 4°C (# Optima-XE SW41 Beckman Coulter), and each pellet were resuspend with 30 μl of dfPBS ...
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bioRxiv - Biochemistry 2020Quote: ... by dounce homogenization (20 strokes) and the homogenate was centrifuged at 55,000 rpm in a Type 70 Ti rotor (Beckman Coulter) for 1 hr ...
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bioRxiv - Genomics 2019Quote: ... Resulting DNA was purified by a 10 min incubation with 70 µl (0.7X) Agencourt AMPure XP beads (A63880, Beckman Coulter), 2x 200 µl washes with 80% ethanol for 30 sec each ...
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bioRxiv - Physiology 2019Quote: ... 70 μl of total plasma was separated by using the Optima MAX-TL Ultracentrifuge with TLA-100 rotor (Beckman Coulter). Plasma lipoproteins were also analyzed by running 200 μl of plasma onto a fast protein liquid chromatography system consisting of Superose6 10/300 GL column (Amersham Pharmacia Biotech) ...
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bioRxiv - Biochemistry 2019Quote: ... The V and VI fractions were then diluted separately to a total volume of 12mL with dfPBS and centrifuged at 100,000 × g for 70 min at 4°C (# Optima-XE SW41 Beckman Coulter). The final pellet from each fraction was resuspended in 30 μL of dfPBS ...
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bioRxiv - Plant Biology 2020Quote: ... The cells were disrupted in a French Press at 1500 psi and centrifuged at 45,000 rpm (Beckman 70 Ti rotor). The supernatant was loaded onto an amylose column (NEB) ...
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bioRxiv - Microbiology 2021Quote: ... 2 ml of each sucrose concentration was layered from 70% to 25% in open-top ultracentrifuge tubes (Beckman Coulter, 344059) immediately before use ...
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bioRxiv - Biochemistry 2019Quote: ... The homogenate was centrifuged for 10 min at 10,000×g at 4°C in a Ti-70 rotor and Optima XPN-90 ultracentrifuge (Beckman Coulter) and the supernatant was removed ...
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bioRxiv - Physiology 2021Quote: ... the supernatant was ultracentrifuged at 100,000 g for 70 min at 4°C using an MLA-55 rotor (Beckman Coulter) and an Optima MAX-TL Ultracentrifuge (Beckman Coulter) ...
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bioRxiv - Cell Biology 2020Quote: ... Tubes were centrifuged twice at 100,000 x g for 70 min at 4°C with a Beckman ultracentrifuge (32Ti rotor from Beckman Coulter). Supernatants were removed and the pellets resuspended in phosphate-buffered saline (PBS ...
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bioRxiv - Molecular Biology 2021Quote: ... lysates were spun for 24 h at 4°C at 50000 rpm in a Type 70 Ti rotor (Beckman Coulter) through a 40 % (w/v ...
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bioRxiv - Bioengineering 2022Quote: ... the conditioned media was subjected to an additional centrifugation step of 118,000 × g for 2 h in a Type 70 Ti ultracentrifuge rotor (Beckman Coulter). The resulting EV pellet was resuspended in 1X PBS and placed into a Nanosep 300 kDa MWCO spin column (Pall ...
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bioRxiv - Cancer Biology 2022Quote: ... The cells were then filtered through a 70 μm strainer and viability was assessed using ViCell cell counter (Beckman-Coulter). Single cell suspensions containing 3×106 cells/sample were stained using appropriate fluorescent antibody cocktails (Listed in Supplementary Table S1 ...
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bioRxiv - Microbiology 2022Quote: ... The sample-containing tube was spun at 347,000 × g and 20°C for 3 hours using Type 70 Ti rotor (Beckman Coulter). 1-mL fractions were collected from the top of the tube ...
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SARS-CoV2 associated secretion of nanoLuciferase reports on virus and Virus-Like Particle productionbioRxiv - Molecular Biology 2022Quote: ... the samples were pelleted through a 20% sucrose in 120 mM HEPES cushion at 100,000xg for 2hrs at 4 °C with 45 Ti or 70 Ti fixed-angle rotor (Beckman-Coulter) depending on the volume of the sample ...
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bioRxiv - Molecular Biology 2023Quote: ... The lysate was clarified at 503,000 rcf for 45 min at 4°C using a Type 70 Ti Rotor (Beckman Coulter). The supernatant was incubated with 3 mL IgG Sepharose 6 Fast Flow beads (Cytiva ...
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bioRxiv - Bioengineering 2023Quote: ... The final supernatant was centrifuged at 100,000g at 4°C for 70 min using an ultracentrifuge (Optima MAX-XP Tabletop Ultracentrifuge, Beckman Coulter). The pellet was either used immediately or stored dry at −80°C.
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bioRxiv - Biochemistry 2023Quote: ... The diluted extract was then centrifuged at 31,200 rpm for 30 minutes at 4°C in a Type 70 Ti rotor (Beckman Coulter). The supernatant was filtered through a 0.45 µm syringe filter (Millex-GP PES membrane (Millipore SLHP033RS ...
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bioRxiv - Biochemistry 2023Quote: ... by dounce homogenization (20 strokes).The lysate was clarified by centrifugation at 56,000 rpm in a Type 70 Ti rotor (Beckman Coulter) at 4°C for 1 hour ...
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bioRxiv - Bioengineering 2023Quote: ... The final supernatant was centrifuged at 100,000g at 4°C for 70 min using an ultracentrifuge (Optima MAX-XP Tabletop Ultracentrifuge, Beckman Coulter). The pellet was either used immediately or stored dry at −80°C ...
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bioRxiv - Molecular Biology 2024Quote: ... Lysates were cleared by centrifugation for 1 h at 45,000 rpm at 4°C (Optima XPN-80 Ultracentrifuge with 70 Ti, Beckman Coulter). HTP-tagged proteins were immunoprecipitated with 600 µl pre-washed IgG-sepharose 6 fast flow affinity resin (GE Healthcare ...
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bioRxiv - Neuroscience 2024Quote: ... were pooled and after ultracentrifugation at 110,000 × g for 70 min (Optima TLX ultracentrifuge with TLA 100.4 rotor, Beckman Coulter, Inc.), the pellets were resuspended in DPBS and stored at -80 °C until further use.
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bioRxiv - Bioengineering 2024Quote: ... The sample tubes were then centrifuged at 347,000 × g and 20°C for 3 hours using a Type 70 Ti rotor (Beckman Coulter). 1-mL fractions were collected from the top of the tube ...
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bioRxiv - Immunology 2019Quote: ... Anti-CD45-PE-Cy7 (I3/2.3) antibody was purchased from Cell LAB (Beckman-Coulter, Brea, CA). Anti-Langerin-AlexaFluor (AF ...
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bioRxiv - Cell Biology 2021Quote: ... platelets in PRP were labeled with a mouse anti-human monoclonal antibody CD61-PE (Beckman Coulter). Platelet activation was detected by direct immunofluorescence labeling of alpha granule release marker CD62P (P-selectin ...
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bioRxiv - Cell Biology 2021Quote: Platelets in PRP were labeled with a mouse anti-human monoclonal antibody CD61-PE (Beckman Coulter). Incubation was performed at room temperature for 10 minutes in the dark ...
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bioRxiv - Neuroscience 2020Quote: ... The supernatant was then collected and subjected to ultracentrifugation at 100,000 x g for 70 min at 4°C using an Optima XPN-80 ultracentrifuge with SW 32 Ti swinging rotor (Beckman Coulter). Pellets were washed in PBS 1X and EVs were subjected to an additional centrifugation at 100,000 x g for 30 min at 4°C using an Optima MAX ultracentrifuge ...