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Citations for Beckman :
101 - 150 of 1038 citations for 14 Nonylphenoxy 3 6 9 12 tetraoxatetradecan 1 ol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... The cAW was collected by spinning at 700 x g for 20 min at 4 ⁰C (JA-14 rotor, Beckman Coulter ...
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bioRxiv - Cell Biology 2023Quote: ... where the sucrose were layered from bottom to top as high to low concentrations in 14×89 mm ultracentrifuge tubes (Beckman, 14×89 mm polypropylene centrifuge tubes ...
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bioRxiv - Microbiology 2023Quote: ... and 5.5 ml was layered on top of a two-step sucrose gradient consisting of 5 ml of 40% sucrose solution layered on top of 1.5 ml of a 65% sucrose solution in a 14 x 89mm Ultra-Clear tube (Beckman Coulter). All sucrose (w/w ...
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bioRxiv - Microbiology 2024Quote: ... tepidum culture was centrifuged at 10,000 x g for 10 min at 4°C in a JA-14 rotor (Beckman Coulter), and the supernatant was centrifuged again at 12,000 x g to remove cells and large debris ...
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bioRxiv - Neuroscience 2022Quote: ... The samples were separated through ultracentrifugation for 1 hr at 200,000 g (spin 3 at 49,000 r.p.m. in Beckman TLS-55 rotor). Top layers of supernatants were removed ...
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bioRxiv - Molecular Biology 2020Quote: ... The library was amplified with 9 PCR cycles and cleaned with 0.9x AMPure® XP beads (A63881, Beckman Coulter, CA, USA) to enrich for DNA fragments shorter than 50nt ...
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bioRxiv - Immunology 2023Quote: Koff rates were determined as dissociation of reversible pMHC-Streptamers upon addition of D-Biotin in a flow cytometry-based assay (CyAn ADP Lx 9 color flow cytometer, Beckman Coulter) as described previously (44) ...
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bioRxiv - Plant Biology 2021Quote: ... Syringes were placed inside 50-mL tubes and centrifuged for 20 min at 700g with slow acceleration (4°C, JA-14 rotor, Avanti J-20 XP Centrifuge; Beckman Coulter). The apoplastic wash fluid was then filtered through a 0.22 μm membrane and centrifuged at 10,000g for 30 minutes to remove any remaining large particles ...
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bioRxiv - Biochemistry 2023Quote: ... and 400 μL was loaded onto a 5-30% (w/v) buffered sucrose gradient (in Buffer B) in a 14 mm × 89 mm thin-wall Ultra-Clear tube (Beckman # 344059) that was formed using a Biocomp Gradient Master ...
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bioRxiv - Plant Biology 2023Quote: ... The cAW was collected by spinning at 700 x g for 20 min at 4 ⁰C (JA-14 rotor, Beckman Coulter; Avanti J-20TM centrifuge, Beckman Coulter). vAW was filtered through a single layer of Miracloth and was combined with the cAW ...
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bioRxiv - Cell Biology 2022Quote: ... and the pellet was resuspended with 5 ml buffer B and transferred into a 14 ml ultracentrifuge tube (331372, Beckman Coulter). 9 ml Buffer C (1.8 M sucrose ...
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bioRxiv - Neuroscience 2024Quote: ... The resulting supernatants were layered onto 1.2M sucrose in 14 x 89 mm Ultra-Clear centrifuge tubes (Beckman, Palo Alto CA). The gradients were centrifuge at 4 °C 160,000 x g for 35 min (SW41 rotor ...
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bioRxiv - Molecular Biology 2023Quote: ... A 10-30% gradient of glycerol in HEMG buffer was poured into 14 x 89 mm polyallomer centrifuge tubes (Beckman #331372). The resuspended nuclear extract was carefully layered on top of the gradient and centrifuged in an SW41 rotor at 40,000 rpm for 16 hours at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... A volume of lysate containing 125 ug RNA was loaded on 11 ml 15-50% sucrose gradients in 14 x 89 mm centrifuge tubes (Beckman Coulter). (Gradients were prepared in 20 mM HEPES-KOH pH 7.4 ...
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bioRxiv - Plant Biology 2024Quote: ... The tubes were then centrifuged for 20 min at 700 x g with slow acceleration at 4°C using a JA-14 rotor (Avanti J-20 XP centrifuge; Beckman Coulter). The resulting apoplastic wash collected was passed through a 0.22 µm membrane filter to remove bacteria and larger debris ...
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bioRxiv - Plant Biology 2024Quote: ... The density gradient was made by transferring the layers sequentially into a tube (Beckman Ultra-Clear 14 x 89 mm, #344059) using a transfer pipet being careful to avoid mixing the layers ...
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bioRxiv - Neuroscience 2024Quote: ... The flow-through was then transferred into 12 mL polypropylene tubes (Beckman Coulter) and centrifuged at 140,000xg (Beckman Coulter Optima L100 XP ...
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bioRxiv - Cell Biology 2022Quote: ... This sonicated fraction was transferred to a 12 ml polycarbonate ultracentrifuge tube (Beckman) and adjusted to 40% (w/v ...
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bioRxiv - Cell Biology 2024Quote: ... for 12 PCR cycles on the liquid handler Biomek i7 (Beckman Coulter GmbH) at GeneCore ...
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bioRxiv - Molecular Biology 2020Quote: ... diluted in 9 mL of 10 mM Tris-HCl buffer and centrifuged for 45 min at 45.000 rpm (Beckman 70.1 Ti Rotor). Pellets were suspended in the same buffer and analyzed by SDS-PAGE using 12.5% polyacrylamide gel ...
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bioRxiv - Cell Biology 2023Quote: ... EV–containing fractions were pooled (1.10–1.18 g/mL) and diluted by adding 9 ml PBS to SW40 tubes (Beckman–Coulter, Fullerton, CA, USA). EVs were pelleted at 200,000xg for 60 min at 4°C (Beckman–Coulter Optima™ L–90K or Optima™ XPN–80 centrifuge ...
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bioRxiv - Biochemistry 2021Quote: ... 6 mM 2-mercaptoethanol and centrifuged with a 70Ti (Beckman coulter) rotor at 40,000 rpm for 18 hours at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... 5×10^6 cells counted by Multisizer 4e Coulter Counter (Beckman) were plated on a 60 mm plate and serum starved before analysis unless otherwise indicated ...
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bioRxiv - Immunology 2024Quote: ... Samples were analyzed on a 6-laser Cytoflex LX (Beckman Coulter) and flow cytometry data were analyzed using FlowJo v.10.9 (BD Life Sciences) ...
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bioRxiv - Cell Biology 2021Quote: ... 100 µg/mL CHX and cOmplete™ EDTA-free Protease Inhibitor Cocktail) prepared in a polypropylene centrifuge tube (14 × 95 mm; Beckman Coulter). A Gradient Master (Biocomp ...
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bioRxiv - Biochemistry 2022Quote: ... 000 g at 4°C for 30 min in a Beckman Coulter Optima L- 100 XP ultracentrifuge using 14 mm × 89 mm Ultra-Clear Centrifuge Tubes (Beckman Instruments Inc.). Ultra-pure mitochondria were harvested at the interphase between 19% and 40% and washed three times with 1xM buffer ...
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bioRxiv - Plant Biology 2023Quote: ... and 2 mL of 5% solution in a 14 x 89 mm (13.2 mL) thin walled ultra-clear centrifuge tubes (Beckman Coulter, Cat. # 344059). Approximately 250 µl of crude EV sample resuspended in 20 mM Tris-HCl pH 6.0 was slowly dispensed on top of the column and centrifuged at 100,000 x g for 17 h at 4 ⁰C (SW 41 Ti rotor ...
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bioRxiv - Molecular Biology 2024Quote: ... ADEV isolation was performed by ultracentrifugation at 100,000 × g for 14 h at 4 °C (swing bucket rotor on minimum acceleration/break setting, SW 32Ti Beckman Coulter, Brea, CA), and ADEVs were resuspended in 200μL PBS and stored at −20°C until used16,41 ...
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bioRxiv - Synthetic Biology 2024Quote: ... 200 μL of supernatant was loaded onto sucrose gradients (10–50% sucrose in 0.6 M KP buffer) prepared in tubes (14 × 89 mm, Open-Top Thinwall Ultraclear tubes [Beckman Coulter, CA, USA]) using a Gradient Master (Beckman Coulter) ...
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bioRxiv - Plant Biology 2020Quote: MP of pollen protoplasts was assessed by staining with 1 µM DiBAC4(3) for 5 min (Breygina et al. 2010) on Gallios flow cytometer (Beckman Coulter), analysis was performed with FlowJo software (TreeStar) ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were then enriched in DNA sizes between 200 and 600 bp (corresponding to 1 to 3 nucleosomes) using AMPure XP magnetic beads (Beckman-Coulter), following the manufacturer’s guidelines ...
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bioRxiv - Cell Biology 2021Quote: ... using 12 mm double sector centerpieces in an AN-50 Ti rotor (Beckman Coulter). Scans were acquired in continuous mode at 280 nm ...
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bioRxiv - Immunology 2022Quote: ... Flow cytometry was performed using a FACSort or FACScalibur flow cytometer (Becton Dickinson, Mountain View, CA) and a CyAN ADP LX 9-colour flow cytometer (Beckman Coulter, Pasadena, CA). Data were analyzed using FlowJo software (Tree Star Inc ...
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bioRxiv - Immunology 2020Quote: ... KIR2DL2/3/S2 (GL183, PE/Cy5.5, Beckman Coulter,), NKG2C (134591 ...
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bioRxiv - Systems Biology 2023Quote: ... enumerated using a Multisizer 3 Coulter Counter (Beckman,), and then seeded at the desired density in 6-well tissue culture plates (TPP ...
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bioRxiv - Developmental Biology 2024Quote: Cleared S10 fractions were adjusted to 500 μL with Subunit Lysis Buffer and overlaid onto 2 mL of a 1 M sucrose cushion prepared in Subunit Buffer in a 15×51 mm 3 mL thickwall polycarbonate ultracentrifuge tube (Beckman Coulter, 349622) on ice ...
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bioRxiv - Microbiology 2022Quote: ... PCR Enrichment of Adaptor Ligated DNA was run with 12 cycles and SPRI beads (Beckman) were used for library clean up ...
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bioRxiv - Immunology 2021Quote: ... Purified cDNA was amplified for 12 cycles before being cleaned up using SPRIselect beads (Beckman). Samples were diluted 4:1 (elution buffer (Qiagen ...
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bioRxiv - Immunology 2023Quote: ... Purified cDNA was amplified for 12 cycles before being cleaned up using SPRIselect beads (Beckman). Samples were diluted 4:1 (elution buffer (Qiagen) ...
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bioRxiv - Cancer Biology 2023Quote: ... ChIP DNA was then amplified 12 cycles and purified using AMpure XP beads (Beckman, A63881) twice and then were submitted for150 bp paired-end sequencing on an Illumina novaseq 6000 platform (Novogene).
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bioRxiv - Plant Biology 2023Quote: ... Supernatant was then filtered through a 0.45 μm sterile syringe filter and 10,4 ml of supernatant were put into an ultracentrifuge tube (Beckman Ultra clear 14 mL tubes (#344085)) ...
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bioRxiv - Plant Biology 2024Quote: ... placed inside 250 mL centrifuge bottles and centrifuged for 10 min at 100 g at 4 °C (JA-14 rotor, Avanti J-20 XP Centrifuge; Beckman Coulter, Indianapolis, IN, USA). LSW was then filtered through a 0.2 µm syringe filter (Acrodisc syringe filter ...
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bioRxiv - Biochemistry 2020Quote: ... disrupted with a tip sonicator at 1/3 power (8 MHz) for 3 × 30 seconds on ice and fractionated by ultracentrifugation (100’000 x g, 1H, Beckman TLA 120.2 rotor). The luminal fraction was concentrated to around 50 µL on a 3 kDa MWCO Amicon Ultra 0.5 mL centrifugal filter while membrane pellets were dissolved in 50 µL TBS with 2% SDS ...
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bioRxiv - Immunology 2021Quote: ... CD38 APC-AF700 (clone LS198-4-3; Beckman Coulter), CD19 APC-Cy7 (clone SJ25C1 ...
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bioRxiv - Synthetic Biology 2021Quote: ... Purified cDNA was amplified for 12 cycles before being cleaned up using SPRI-select beads (Beckman). Samples were diluted 4 times in water and ran on a Bioanalyzer (Agilent Technologies ...
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bioRxiv - Immunology 2022Quote: ... For determination of total cell concentrations Flow-12 Count™ Fluorospheres (Beckman Coulter Inc., Krefeld, Germany) was used.
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bioRxiv - Biochemistry 2024Quote: ... 15 µL of the product was mixed thoroughly with 12 µL Ampure XP beads (Beckman Coulter) (0.8× bead/PCR product ratio ...
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bioRxiv - Microbiology 2021Quote: ... 2.5 ml of each solution were used to form the gradient in a 14 ml Ultra-Clear™ centrifuge tube (Beckman Coulter, Brea, CA, USA), with the highest sucrose concentration at the bottom of the tube ...
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bioRxiv - Cell Biology 2020Quote: ... amplified by PCR for 6 cycles and purified with AMPure XP beads (Beckman Coulter). The final libraries were pooled and sequenced using the onboard cluster method ...
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bioRxiv - Genomics 2020Quote: ... (6) the library fragments were purified with AMPure XP Beads (Beckman Coulter, Shanghai, China) to select the insert fragments of 150-200 bp in length ...