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Citations for Beckman :
1401 - 1450 of 1915 citations for 6 Amino 5 2 chloro 4 nitrophenyl azo naphthalene 1 sulfonamide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... the supernatant of cell lysates was centrifuged to isolate the total membrane fraction at 24,000 rpm for 2 h in the 45Ti rotor using ultracentrifuge (Beckman-Coulter). Membrane resuspension in 50 mM Tris-HCl buffer (pH 8.0 ...
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bioRxiv - Cell Biology 2023Quote: ... The supernatant was then overlaid onto a 2 ml 60% iodixanol cushion and spun at 100,000 x g (SW32 rotor, Beckman Coulter) for 18h ...
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bioRxiv - Neuroscience 2023Quote: ... This supernatant was next subjected to ultracentrifugation at 120,000 x g for 2 h at 4LJ in a SW 41 Ti Rotor Swinging-Bucket rotor (Beckman Coulter), and the pellets collected as small factors like exosome ...
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bioRxiv - Microbiology 2023Quote: ... resuspended in 200 µl 2% PBS-BSA and analyzed for mean fluorescence intensity (MFI) of ACE2 expression with CytoFLEX flow cytometer (Beckman). To determine the biological effects of the purinergic receptor P2X7 and NLRP3 inflammasome on viral entry ...
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bioRxiv - Biophysics 2023Quote: ... followed by layering the cleared lysate onto 40 mL of 2 M sucrose cushion and ultracentrifugation in Ti-45 rotor (Beckman) at 42,000 rpm for 2 hours ...
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bioRxiv - Neuroscience 2023Quote: ... Fraction LP2 was obtained after centrifugation of fraction LS1 for 2 h at 165,000 g in a SW40 Ti rotor (Beckman, US). Corresponding supernatant was collected as Fraction LS2 ...
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bioRxiv - Plant Biology 2023Quote: ... and water to a final volume of either 2 μL for the acoustic liquid handling robot (Labcyte Echo 550, Beckman) or 5 μL for manual handling ...
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bioRxiv - Immunology 2023Quote: The SARS-CoV-2 lentiviral pseudotyped neutralization assays were performed on integrated automation platforms consisting of a Biomek liquid handler from Beckman Coulter ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were ultracentrifuged at 4 °C and 150,000 ˗ g for 2 h (Optima L-80 XP Ultracentrifuge with Type 70.1 Ti Fixed-Angle Titanium Rotor (Beckman Coulter)) and supernatants were removed ...
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bioRxiv - Biochemistry 2022Quote: ... Around 100 µL of sample at 15 µM was applied to each gradient and centrifuged by ultracentrifugation at 45 000 rpm at 4°C in SW 60 Ti Swinging-bucket rotor (Beckman Coulter, Palo Alto, US-CA) for 16 hours ...
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bioRxiv - Microbiology 2020Quote: ... Prepared phage suspension was transferred to U-formed centrifuge tubes suitable for ultra-centrifugation (25 000 × g, 120 min, 4 °C) (Beckman Coulter, Optima™ MAX-XP). Pellets were resuspended in 200 µL of SM buffer and phage stock suspensions were stored at 4°C and −80°C.
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bioRxiv - Neuroscience 2019Quote: ... The ultracentrifuge tubes were centrifuged at 124000 x g at 4 ° C for 30 minutes in Optima L-100XP ultracentrifuge (Beckman Coulter, SW 55 Ti rotor). The resulting pellet was suspended in 50 μL of TKM with protease inhibitors and commercial phosphatases (78440 ...
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bioRxiv - Cell Biology 2021Quote: ... This lysate was sonicated for 2 min (15 s on and 20 s off for 2 min at 20% amplitude) and centrifuged at 100,000 x g for 40 min at 4°C (Avanti J-301 Beckman Coulter centrifuge with rotor JA-30.50). The supernatant was filtered and passed through preequilibrated 5 ml HisTrap FF columns (GE Healthcare) ...
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bioRxiv - Biophysics 2022Quote: ... Labeled G-actin was clarified to remove any remaining F-actin or precipitated FMAL by centrifugation (10 min, 90,000 rpm in a Beckman TLA-120.2 rotor at 4°C). The extent of labeling (typically around 70% ...
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bioRxiv - Biophysics 2023Quote: ... Cells were harvested by centrifugation at 8000 g at 4°C for 20 min (Avanti® J-E centrifuge from Beckman Coulter, rotor: JLA 16.250).
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bioRxiv - Bioengineering 2023Quote: ... subtilis spores were collected from the culture by the centrifugation at 8000 rpm at 4 °C for 20 min (Avanti J-E, Beckman Coulter Life Science, IN, USA). Supernatant was removed and the spore pellets were resuspended in fresh 100 mM PBS pH 7.4 ...
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bioRxiv - Biochemistry 2023Quote: ... lysosomes and mitochondria were enriched by centrifugation of the pooled PNS at 56,500 ×g for 7 min at 4 °C (Beckman-Coulter, 70 Ti fixed-angle rotor). The supernatant was removed ...
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bioRxiv - Biophysics 2021Quote: ... Different ratios of LUVs and cyt c (with the protein concentration kept at 5 μM) were incubated in the sample buffer at room temperature for 15 min in ultracentrifugal tubes (Beckman Coulter, Indianapolis, IN). The effective PG/cyt c molar ratio (R ...
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bioRxiv - Cell Biology 2019Quote: ... The mix was then airfuged 5 min at 30 psi with an Airfugee® Air-driven ultracentrifuge (Beckman Coulter Inc., Brea, California, USA) and the pellet was re-suspended in 80% of the initial volume and left on ice for 20 min to depolymerize the microtubules ...
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bioRxiv - Immunology 2021Quote: ... Supernatants were collected and centrifuged for 5 min at 1200 rpm at 20°C (Allegra™ X-12R Centrifuge, Beckman Coulter, the Netherlands). Cells were then resuspended in PBS ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were pelleted and washed twice before resuspension in 500μL of cold PBS for analysis using flow cytometry (CytoFLEX 5, Beckman Coulter, Mississauga, ON, Canada) wherein mean fluorescence intensity of the gated cells (10,000 events ...
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bioRxiv - Evolutionary Biology 2022Quote: ... We pooled 1.5 µl of each indexed library (including blanks and museum controls) and performed size selection with AMPure XP beads (Beckman Coulter, IN, USA) for fragments of approximately 100-500 bp in length ...
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bioRxiv - Cell Biology 2021Quote: 1 mL samples of culture were diluted 1:100 diluted with Isoton II Diluent (Beckman Coulter) and used to determine cell concentration of the culture using a Beckman Multisizer 3 Coulter Counter ...
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bioRxiv - Genomics 2022Quote: ... The DNA fragments were purified by Ampure XP beads (volume ratio 1:1, Beckman Coulter, #A63881) and elute the DNA fragments in 27 µL water ...
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bioRxiv - Cancer Biology 2023Quote: ... Post-PCR clean-up was performed using 1:1 volume Ampure XP beads (Beckman Coulter A63880). Samples were dissolved in 10 mM Tris-HCl (pH 8 ...
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bioRxiv - Biochemistry 2021Quote: ... 1 h 10 min (45Ti, Beckman), resuspended in lysis buffer and stored in aliquots at –80°C until use ...
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bioRxiv - Bioengineering 2020Quote: ... 1X 1 μm SPRI beads (Beckman) in 1X PBS buffer with 0.5% Tween20 were injected into the thin-chip ...
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bioRxiv - Cancer Biology 2020Quote: ... GlyA PE (1:100, Beckman Coulter), CD71 BV650 (1:100) ...
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bioRxiv - Cancer Biology 2020Quote: ... GlyA PE (1:100, Beckman Coulter), CD71 BV650 (1:100) ...
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bioRxiv - Cancer Biology 2020Quote: ... CD4 PC5 (1:100, Beckman Coulter) and CD56 BV605 (1:100) ...
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bioRxiv - Cancer Biology 2020Quote: ... GlyA PE (1:100, Beckman Coulter), CD71 BV650 (1:100) ...
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bioRxiv - Microbiology 2022Quote: ... or 1:300 KC57-RD1 (Beckman Coulter 6604667 in BD Perm/Wash Buffer for 30 minutes ...
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bioRxiv - Immunology 2023Quote: ... CD56 (NKH-1-RD1, Beckman Coulter), ERK1/2 (T202/Y204 ...
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bioRxiv - Cell Biology 2020Quote: ... thin-layer cell preparations for confocal microscopy of enriched small luteal cells and large luteal cells was performed using Cytofuge 2 (Beckman Counter). Cells were then fixed with 10% neutral buffered formalin at 4 °C for 30 min ...
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Phenotypic and molecular evolution across 10,000 generations in laboratory budding yeast populationsbioRxiv - Evolutionary Biology 2020Quote: ... We combined the 8 wells from column 2 of each plate and used the pooled culture to measure cell density on a Coulter Counter Z2 (Beckman Coulter). We spotted the entire volume of each of the other 88 wells on CSM-Arg (Sunrise Scientific ...
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bioRxiv - Genomics 2020Quote: The ∼400 bp amplicon product produced using both Pool-1 and Pool-2 from the same clinical sample was combined and purified using a 1X concentration of Agencourt AMPure XP beads (Beckman Coulter); the final product was eluted in 30 µl of elution buffer (Qiagen ...
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bioRxiv - Neuroscience 2019Quote: ... with 2 ml of a 20% sucrose in dPBS cushion and ultracentrifugated at 20,000 RPM (Beckman Coulter SW 32 Ti rotor) at 4°C for 2 hours ...
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bioRxiv - Cell Biology 2019Quote: ... The culture was harvested in to 2 × 250 ml centrifuge bottles (Nalgene, 3120-0250) and centrifuged in an Optimia XPN-100 ultra centrifuge (Beckman Coulter) with a SW 32 Ti rotor at 5,000 × g for 20 min at 4 °C ...
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bioRxiv - Cancer Biology 2020Quote: ... The donor-derived LT-HSC cell populations were sorted according to the gating strategy in Extended data Figure 2 using MoFlo Astrios Cell Sorter (Beckman Coulter). LT-HSC cells were directly sorted into 50μl RNA extraction buffer (ARCTURUS PicoPure RNA Isolation Kit (Applied Biosystems ...
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bioRxiv - Cancer Biology 2022Quote: ... 10 nM leptomycin b, vehicle control or library compounds (Supplementary Tables 2, 3) were added using an Echo liquid handler (Labcyte/Beckman Coulter). Cells were incubated for a further 24 h then fixed in 4% paraformaldehyde and stained with DAPI and Alexa Fluor 488 Phalloidin (ThermoFisher) ...
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bioRxiv - Microbiology 2022Quote: ... Washed pellets were resuspended in 2 mL 1x PBS and fluorescence was calculated using a LSR II cell analyzer (Beckman Coulter). Two biological replicates of 50,000 cell counts were analyzed with FlowJo ...
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bioRxiv - Neuroscience 2020Quote: ... the homogenized solution was added onto a sucrose cushion (2 ml) in a 10 ml Ultra-Clear centrifuge tube (Beckman Coulter). Separation of nuclei from the tissue was done by centrifugation (26.500xg at 4°C for 1.5 h ...
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bioRxiv - Microbiology 2022Quote: ... filtered supernatant was underlaid with 20% sucrose PBS using a pipetting needle and centrifuged at 39 000 rpm for 2 h using an SW-41 rotor (Beckman Coulter). The supernatant and sucrose solution were carefully removed ...
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bioRxiv - Microbiology 2022Quote: ... The virus suspension was pelleted through a 40% sucrose cushion by centrifugation for 2 h at 40,000 rpm in a SW55 Ti rotor (Beckman, Fullerton, CA) at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... Samples were incubated for 2 h at 16°C and double stranded cDNA was purified using AMPure XP beads (Beckman Coulter). For testing transcription activity of predicted genes ...
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bioRxiv - Genomics 2019Quote: ... Cleared cell extracts were ultracentifuged at 34400 rpm for 2 hours 40 minutes at 4C using a C-1000 XP centrifuge with SW40 rotor (Beckman Coulter). Gradient UV absorption at 254 nm was measured and selected fractions were selected for 5’cap library preparation (5µg purigied RNA per sample) ...
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bioRxiv - Developmental Biology 2019Quote: Detection of apoptotic TCam-2 cells was performed 48 h after transfection of TCam-2 cells with control and KIF18A siRNA constructs using an Annexin V-FITC Apoptosis Detection Kit (Beckman Coulter), according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... to remove cellular debris and purified by ultracentrifugation over a 25% sucrose cushion (28.000 rpm for 2 hours; Beckman Coulter ultracentrifuge).
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bioRxiv - Cancer Biology 2021Quote: ... Cells were treated with 3 different doses of the conventional chemotherapy for 2 days and cell viability was assessed using a Vi-cell XR Viability Analyzer (Beckman Coulter); 6 replicates were performed for each cell line and each dose.
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bioRxiv - Microbiology 2019Quote: ... PCR products were verified with a 2% agarose gel electrophoresis and purified using AMPure XP bead (Beckman Coulter, Brea, CA, USA). The purified amplicon library was quantified and sequenced on the MiSeq Platform (Illumina ...