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Citations for Beckman :
1401 - 1450 of 2068 citations for 6 9 Methano 4 1 benzoxazepin 2 3H one octahydro 3 methyl 3 α 5a bta 6 bta 9 bta 9a bta 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... Saliva was collected by expectoration into tubes on ice followed by centrifugation for 10 minutes at 4°C at 4000 x g (Beckman SX4750 rotor) to remove large debris and bacterial aggregates ...
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bioRxiv - Cell Biology 2024Quote: ... The collected monosomes were then 1:1 diluted with PEB and centrifuged at 60,000 rpm at 4°C overnight on an SW65 Ti ultracentrifuge rotor (Beckman Coulter, California, USA). The pellets were resuspended in 250µl PEB with 50 mM NH4Cl ...
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bioRxiv - Biochemistry 2023Quote: ... Cultures were then recovered by centrifugation (4,000 x g, 4 °C, 40 min in a Beckman Avanti JXN-26 (rotor: JLA 8.1)) and pellets resuspended in approximatively 30 mL of lysis buffer (20 mM HEPES ...
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bioRxiv - Molecular Biology 2024Quote: ... ADEV isolation was performed by ultracentrifugation at 100,000 × g for 14 h at 4 °C (swing bucket rotor on minimum acceleration/break setting, SW 32Ti Beckman Coulter, Brea, CA), and ADEVs were resuspended in 200μL PBS and stored at −20°C until used16,41 ...
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bioRxiv - Cell Biology 2023Quote: ... Following centrifugation at 131,300 × g for 24 hr at 4 °C using a MLS-50 Swinging-Bucket rotor (Beckman Coulter, Brea, CA) to sediment protein complexes ...
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bioRxiv - Cell Biology 2024Quote: ... All centrifugation steps were done at 4°C and the last two steps were performed using Optima XE-90 ultracentrifuge (Beckman Coulter, A94471) with rotor SW32Ti (Beckman Coulter ...
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bioRxiv - Cell Biology 2024Quote: ... and cells were isolated by centrifugation at 500 g for 5 min at 4°C followed by being treated with a red blood lysis buffer (Beckman Coulter, USA). After washing with PBS ...
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bioRxiv - Microbiology 2023Quote: ... The lysate was clarified by centrifugation at 5500 rpm for 20 min at 4°C (AllegraTM 21R Centrifuge, Beckman Coulter, CA, USA). The sample was then loaded into a HisTrap HP 5 mL column (Cytiva ...
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bioRxiv - Neuroscience 2023Quote: ... then spun at 100,000 x g for 30 min at 4°C in an ultracentrifuge (Beckman OPTIMA TLX with TLA-100.3 rotor). The supernatant was saved and the pellet went through a series of similar extractions using High-Salt Triton Buffer (High-Salt Buffer ...
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bioRxiv - Neuroscience 2023Quote: ... The supernatants were then combined and centrifuged at 17,000g for 10min at 4°C in a Beckman SW41-Ti rotor (Beckman Coulter, Brea, CA). The supernatant was saved as the cytosolic (CYT ...
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bioRxiv - Cell Biology 2023Quote: Myf5High and Myf5Low MuSCs were isolated from a pool of 4 male and 4 female mice and repeated in two independent experiments and total RNA was extracted using Agencourt RNAdvance tissue Kit (Beckman Coulter, #A32646) with a quality score of RQN 9.3 to 10 ...
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bioRxiv - Cell Biology 2023Quote: ... was next subjected to ultracentrifugation at 120,000 × g for 4 h in a SW 28 Ti Swinging-Bucket Rotor (Beckman Coulter, Fullerton, CA) to sediment small EVs ...
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bioRxiv - Biochemistry 2023Quote: ... Triton-X was added to the lysate to a final concentration of 0.1% and clarified by spinning at 15,000 RCF at 4°C for 45 minutes (Beckman Coulter JA-20 rotor). The supernatant was loaded onto a 5 mL HisTrap™ FF crude column (GE Healthcare ...
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bioRxiv - Microbiology 2023Quote: ... Cells were incubated further overnight at 20°C and subsequently harvested by centrifugation (Beckman rotor 8.1000, 5000 rpm, 15 min, 4°C). The pellet was resuspended in PBS ...
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bioRxiv - Biochemistry 2023Quote: ... by centrifugation at 80,000 × g for 30 min at 4°C using a TLS-55 rotor (Beckman Coulter Inc., Miami, FL, USA). Crista membranes were identified and removed based on fluorescence under a UV lamp ...
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bioRxiv - Developmental Biology 2024Quote: ... for 30 min at 4°C in the dark followed by flow cytometry analysis on a CytoFlex 26 (Beckman Coulter, Indianapolis, IN) in PE-A and PerCP-A channels ...
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bioRxiv - Biophysics 2024Quote: ... Samples were centrifuged at 24,000 rotations per minute using an S-28 rotor at 4◦C in an Optima XL-100K Ultracentrifuge (Beckman Coulter, Brea, CA). The supernatant was decanted ...
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bioRxiv - Molecular Biology 2024Quote: ... was loaded on top of the gradient and spun at 4°C for 18 hr at 30,000 rpm (Beckman, Rotor SW-41Ti). Samples were fractionated with absorbance optics (λ = 260 nm ...
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bioRxiv - Genetics 2021Quote: ... and samples were evaluated 2 minutes later with a Cytomics FC500 flow cytometer (Beckman Coulter, Brea, CA, USA) AO was excited with a 488 nm argon laser ...
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bioRxiv - Cancer Biology 2020Quote: ... concentrated for 2 h by ultracentrifugation in a swing-out rotor SW-32Ti (Beckman Coulter, Brea, US-CA) at 26000 g ...
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bioRxiv - Physiology 2019Quote: ... plasma samples were deproteinized with ZnSO4 and Ba(OH)2 and counted using a Liquid Scintillation Counter (Beckman Coulter LS6500 Multi-purpose Scintillation Counter) ...
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bioRxiv - Cell Biology 2022Quote: ... Lentiviral particles were then concentrated by centrifugation for 2 hours at 20,000 rcf (Beckman Coulter, Optima L-100K) in round bottom polypropylene tubes (Beckman Coulter ...
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bioRxiv - Cell Biology 2022Quote: Total RNA of Caco-2 cells and human ileum organoids was extracted using RNAdvance Tissue kit (Beckman Coulter) and total RNA of mouse organoids was isolated using Quick-RNA Miniprep Plus Kit (Zymo Research) ...
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bioRxiv - Microbiology 2024Quote: ... (The filtrate was subjected to ultracentrifugation at 100,000g for 2 h (Optima L-90 K ultracentrifuge; Beckman Coulter). The supernatant was discarded and the pellet was washed with sterile PBS and filtered through a sterile 0.2 μm-pore polyvinylidene difluoride (PVDF ...
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bioRxiv - Microbiology 2023Quote: ... The filtrate was subjected to ultracentrifugation at 200,000xg for 2 h (Optima L-100 XP ultracentrifuge; Beckman Coulter). Supernatants were discarded ...
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bioRxiv - Physiology 2024Quote: ... was analysed via a sequential 2-step immune-enzymatic assay (inter-assay CV 5.2-7.8%) according to manufacturer’s instructions (Beckman Coulter) and follicle stimulating hormone (FSH ...
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bioRxiv - Genomics 2021Quote: ... then re-purified with SPRI beads with 1:1 ratio (Beckman Coulter, B23317), and eluted in 25 µl of water (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... by ultra-centrifugation in a Beckman L7-65 ultracentrifuge at 25,000 rpm for two hours at 4°C using a Beckman SW28 rotor (Beckman Coulter, Brea, CA, USA). Supernatants were aspirated off and the pellets were re-suspended in PBS (pH 7.4) ...
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bioRxiv - Microbiology 2020Quote: ... by centrifugation in a Beckman L7-65 ultracentrifuge at 25,000 rpm for 2h at 4°C using a Beckman SW28 rotor (Beckman Coulter, Brea, CA, USA). Supernatants were aspirated and the pellets were re-suspended in PBS (pH 7.4) ...
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bioRxiv - Molecular Biology 2021Quote: ... The centrifugation is performed on a SW60Ti rotor at 40 000 g for 22 minutes at 4°C (Beckman Optima L-90K Ultracentrifuge). The mitochondrial fraction (400µL ...
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bioRxiv - Molecular Biology 2020Quote: ... and plasma was separated by centrifugation (2,000 × g, 20 min, 4 °C) in a Microfuge 20R (cat. no. B30149, Beckman Coulter, Brea, CA, USA). Plasma was transferred to new 1.5 mL microcentrifuge tubes and further clarified by centrifugation (10,000 × g ...
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bioRxiv - Biophysics 2022Quote: ... shortly sonified and centrifuged for 30 min at 100 000 g at 4°C (Centrifuge Beckman Coulter Optima XPN-80, Rotor type 70.1).) ...
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bioRxiv - Biophysics 2022Quote: ... The samples were centrifuged for 30 min at 100 000 g at 4°C (Centrifuge Beckman Coulter Optima XPN-80, Rotor type 70.1). The supernatant ...
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bioRxiv - Microbiology 2020Quote: ... CLP assembly reactions were incubated at 25°C for 30 min before loading onto 15-30% (wt/wt) sucrose gradients and spinning at 159,599 X g for 43 min at 4°C (Beckman Coulter TLS 55 rotor). Gradients were immediately fractionated and aliquots of each fraction were denatured by boiling in 1% SDS to ensure antibody access ...
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bioRxiv - Biochemistry 2020Quote: ... Unbroken cells were pelleted by centrifugation at 18,000 RCF for 30 min at 4°C (Beckman centrifuge J-20-XP, Rotor JA16.250, DE). The supernatant was transferred to 45 TI rotor (Beckman ...
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bioRxiv - Bioengineering 2020Quote: ... and the supernatant was centrifuged again at 26000 × g at 4 °C for 15 min using an Optima L-90K (Beckman Coulter, Brea, CA) ultracentrifuge with a 70-Ti fixed angle rotor (Beckman Coulter) ...
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bioRxiv - Biochemistry 2021Quote: ... supernatants were collected and centrifuged with 150.000 x g (at rav) for 30 min at 4 °C using an Optima™ MAX-XP Ultracentrifuge (Beckman Coulter; TLA120.2 rotor). Pellets were resuspended in PBS and 4 µg of primary antibody were added for 30 min ...
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bioRxiv - Genomics 2021Quote: ... The spores were washed twice with sterile ddH2O and pelleted at 3700g for 5 min at 4 °C in an Allegra X-12R centrifuge (Beckman, Brea, CA, USA). Spores were resuspended in sterile ddH2O and kept at 4°C until experiments.
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bioRxiv - Neuroscience 2021Quote: ... The resulting lysates were centrifuged at 100,000Xg for 30-60 min at 4°C in an Optima TLX tabletop ultracentrifuge using a TLA-110 rotor (Beckman Coulter, Fullerton, CA). The clarified supernatants were removed and transferred into the new tubes ...
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bioRxiv - Biochemistry 2020Quote: ... Unbroken cells were pelleted by centrifugation at 18,000 RCF for 30 min at 4°C (Beckman centrifuge J-20-XP, Rotor JA16.250, DE). The supernatant was transferred to 45 TI rotor (Beckman ...
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bioRxiv - Bioengineering 2021Quote: ... The samples were then centrifuged at 12,000 g for five minutes at 4°C to remove tissue debris with a Microfuge™ 22R centrifuge (Beckman Coulter, Brea, CA). The RNA was extracted using the Invitrogen PureLink™ RNA Mini Kit (Thermo Fisher ...
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bioRxiv - Bioengineering 2021Quote: ... The tubes were then centrifuged at 1000 g for 15 minutes at 4°C using a Microfuge™ 22R (Beckman Coulter, Brea, CA), and the serum was then stored at -80°C ...
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bioRxiv - Microbiology 2021Quote: ... resuspended in 150 μl 0.1 M HEPES/NAOH pH 7.0 and purified for 48 h (250,000 × g, 4 °C; Beckman Optima LE-80K, rotor SW60-Ti) using a CsCl gradient (0.45 g/ml ...
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bioRxiv - Molecular Biology 2020Quote: ... and centrifuged at 10,000xg for 30 minutes at 4°C in a Beckman Coulter Optima XE-100 (fitted with a SW41Ti Rotor (Beckman Coulter, CA USA)) ...
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bioRxiv - Biophysics 2022Quote: ... EVs were fractionated by OptiPrep density gradient ultracentrifugation (100,000 × g, 18 h, 4 °C) using a SW41 rotor (Beckman Coulter, Fullerton, CA, USA) through a continuous 5–40% OptiPrep (Sigma-Aldrich ...
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bioRxiv - Immunology 2022Quote: ... followed by ultracentrifugation at 110,000 × g for 90 min at 4°C on an Optima XPN -100 ultracentrifuge with SW32Ti rotor (Beckman Coulter, Brea, CA, USA). The isolated EVs was washed with PBS and followed with a secondary ultracentrifugation (110,000 × g for 90 min at 4°C) ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were spun down at 4°C for 15 minutes at 4000 RPM (3040 RCF) in a J6 centrifuge (Beckman Coulter Life Sciences). Supernatant was discarded and pellets were stored in 15ml conical tubes in -80°C ...
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bioRxiv - Biochemistry 2020Quote: ... Unbroken cells were pelleted by centrifugation at 18,000 RCF for 30 min at 4°C (Beckman centrifuge J-20-XP, Rotor JA16.250, DE). The supernatant was transferred to 45 Tl rotor (Beckman ...
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bioRxiv - Microbiology 2021Quote: ... Ten milliliters of allantoic or amniotic fluid was centrifuged at maximum speed (16,000 × g) for 30 min at 4 °C using a floor ultra centrifuge (Beckman Optima L-60 Ultracentrifuge). After removal of the supernatant ...
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bioRxiv - Microbiology 2021Quote: ... the SBV samples were purified via sucrose density gradient centrifugation (10–50% range) at 32,500 rpm for 4 h with an SW 41 Ti Swinging-Bucket Rotor (Beckman Coulter Inc., Brea, CA). The SBV band that corresponded to the 40% sucrose gradient was harvested and then centrifuged at 25,000 rpm for 12 h ...