Labshake search
Citations for Beckman :
1301 - 1350 of 2165 citations for 6 Methyl 5 4 phenyl 1 3 thiazol 2 yl 2 trifluoromethyl nicotinic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2023Quote: ... Gradients were centrifuged at 4°C for 17 h at 100,000 g using an L7 ultracentrifuge with an SW28 swing out rotor (Beckman Coulter). Fractions (F ...
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bioRxiv - Cell Biology 2023Quote: ... The iodixanol density gradients were centrifuged at 100,000 x g at 4◦C for 18 hours in SW41Ti rotor (Beckman Coulter). Ten fractions (F1 ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were cleared by centrifugation at 25 000 rpm for 45 min at 4°C in a Ti45 rotor (Beckman). Supernatant was incubated with 5 ml of Glutathione Sepharose 4B beads slurry (Cytiva ...
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bioRxiv - Neuroscience 2022Quote: ... The homogenised mixture then underwent ultracentrifugation at 200,000 g for 110 min at 4°C with a SW-41 Ti rotor (Beckman Coulter) and transferred the supernatant into a Slide-A-Lyzer™ G2 Dialysis Cassettes 2K MWCO (Thermo ...
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bioRxiv - Cancer Biology 2023Quote: ... clarified by centrifugation at 20,000 rpm (48,385 x g) for 30 min in a JA25.50 rotor at 4 °C (Beckman AvantiTM J-25), filtered through a 0.8/0.2 AcroPak Filter Unit (Pall) ...
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bioRxiv - Genomics 2023Quote: ... the supernatant was cleared by two rounds of centrifugation at 20,000 ×g (10 min, 4 °C, JA-20 rotor, Avanti J-26 XP, Beckman Coulter) followed by centrifugation at 31,900 ×g (10 min ...
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bioRxiv - Genomics 2023Quote: ... Precipitated ribosomes were pelleted at 20,000 ×g (10 min, 4 °C, JA-20 rotor, Avanti J-26 XP, Beckman Coulter). To remove residual supernatant the pellet was re-centrifuged for 5 min at 20,000 ×g ...
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bioRxiv - Molecular Biology 2023Quote: ... then pelleted at 4°C and 4,300 x g for 30 min in an Allegra X-14R centrifuge (Beckman Coulter) equipped with a swinging bucket SX4750 rotor ...
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bioRxiv - Plant Biology 2023Quote: ... The cAW was collected by spinning at 700 x g for 20 min at 4 ⁰C (JA-14 rotor, Beckman Coulter ...
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bioRxiv - Genomics 2023Quote: ... The ribosomes were further purified by centrifugation at 59,727 ×g (15 hours, 4 °C, SW 32.1 Ti rotor, Optima™ L-100 XP, Beckman Coulter) in a linear (15 – 30 % ...
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bioRxiv - Systems Biology 2023Quote: ... The urine was centrifuged at 20,000 x g for 30 minutes at 4°C (k-factor 1,790) in an Optima XPN-80 ultracentrifuge (Beckman Coulter) equipped with a SW32 Ti swinging bucket rotor (Rmin 67 ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were lysed by sonication and the lysate was clarified at 150,000 rcf for 30 min at 4°C using a Type 45 Ti Rotor (Beckman Coulter). The lysate was passed three times though 2mL of pre-equilibrated Ni-NTA Agarose beads (Qiagen ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were lysed by sonication and the lysate was clarified at 150,000 rcf for 30 min at 4°C using a Type 45 Ti Rotor (Beckman Coulter). The lysate was passed three times through 2 mL of pre-equilibrated Ni-NTA Agarose beads (Qiagen ...
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bioRxiv - Molecular Biology 2023Quote: ... The lysate was clarified at 150,000 rcf for 45 min at 4°C using a Type 45 Ti Rotor (Beckman Coulter). The supernatant was incubated with 3 mL IgG Sepharose 6 Fast Flow beads (Cytiva ...
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bioRxiv - Molecular Biology 2023Quote: ... The lysate was clarified at 150,000 rcf for 30 min at 4°C using a Type 45 Ti Rotor (Beckman Coulter). The supernatant was incubated with 3 mL IgG Sepharose 6 Fast Flow beads (Cytiva ...
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bioRxiv - Molecular Biology 2023Quote: ... The lysate was clarified at 503,000 rcf for 45 min at 4°C using a Type 70 Ti Rotor (Beckman Coulter). The supernatant was incubated with 3 mL IgG Sepharose 6 Fast Flow beads (Cytiva ...
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bioRxiv - Cell Biology 2023Quote: ... the post-nuclear supernatant was centrifuged for 30 min at 100,000 x g at 4°C using a S120AT2 rotor (Beckman Coulter). The resulting pellet containing the membranes was resuspended in SDS sample buffer (see below).
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bioRxiv - Cell Biology 2023Quote: ... 355647) and centrifuged at 35,000 rpm (100,000xg) for 40min at 4°C (Fixed-angle rotor, Beckman Coulter Optima Max Unltracentrifuge). 100K pellet (sEVs ...
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bioRxiv - Plant Biology 2023Quote: ... Approximately 250 µl of crude EV sample resuspended in 20 mM Tris-HCl pH 6.0 was slowly dispensed on top of the column and centrifuged at 100,000 x g for 17 h at 4 ⁰C (SW 41 Ti rotor, Beckman Coulter; OptimaTM L-90K, Beckman Coulter). After the ultracentrifugation ...
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SARS-CoV2 associated secretion of nanoLuciferase reports on virus and Virus-Like Particle productionbioRxiv - Molecular Biology 2022Quote: ... the samples were pelleted through a 20% sucrose in 120 mM HEPES cushion at 100,000xg for 2hrs at 4 °C with 45 Ti or 70 Ti fixed-angle rotor (Beckman-Coulter) depending on the volume of the sample ...
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bioRxiv - Molecular Biology 2022Quote: ... and the sucrose cushion was ultra-centrifuged for 1.5 hours at 80000 rpm and 4°C in TLA-100 rotor (Beckman Coulter). The pellet was resuspended in 100 µL of buffer F (50 mM HEPES-KOH pH 7.4 ...
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bioRxiv - Molecular Biology 2024Quote: ... the rest was loaded on top of sucrose gradient and ultracentrifuged (30000 rpm, 4°C, 18h, stop DECEL, slow, SW40/1Ti swinging bucket rotor, Optime L-90K, Beckman). 24 fractions were taken from top to bottom of the tube as 500 µL aliquots and precipitated by incubation with 50 µL of 0.15% sodium deoxycholate for 10 min at RT ...
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bioRxiv - Genomics 2023Quote: ... 1x protease inhibitor cocktail EDTA-free) by spinning at 38,000 rpm at 4 °C in a SW-41 Ti rotor (Beckman Coulter). Monosome pellets were resuspended in 400 µL lysis buffer and rocked overnight ...
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bioRxiv - Biophysics 2024Quote: ... and subjected to ultracentrifugation at 384,492□g for 45□min at 4□°C (Beckman Coulter Optima MAX, TLA-100 rotor), stored at −80 °C after flash-frozen with liquid nitrogen for crystallization trials ...
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bioRxiv - Biochemistry 2024Quote: ... The protein samples were carefully applied to the gradient and centrifuged for 16 h at 130,000 g at 4 °C (Optima XPN-80 Ultracentrifugation from Beckman Coulter). 200 µl fractions were taken from the top of the gradient and the glutaraldehyde quenched with 20 mM Tris pH 7.5 ...
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bioRxiv - Biochemistry 2024Quote: ... The homogenate was clarified by centrifugation for 30 min at 70,560 × g at 4°C in an Optima XPN Ultracentrifuge (Beckman Coulter) using a Ti-45 rotor ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were centrifuged at 30,000 xg for 45 min at 4 °C using a SW41 Ti swinging bucket rotor (Beckman Coulter) before the supernatant was decanted ...
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bioRxiv - Neuroscience 2024Quote: ... The homogenate was centrifuged (45 min, 16 000 rpm, 4°C, JLA 16.250 rotor, Beckman-Coulter Centrifuge Avanti J25-01) and the supernatant subjected to Ni-NTA affinity purification (HIS-Select Nickel Affinity Gel (Sigma Aldrich)) ...
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bioRxiv - Biochemistry 2023Quote: ... The gradient was centrifuged for 2.30 hours at 4 °C and 111,000 ×g in a swing-out rotor (Beckman- Coulter, SW41). The brownish lysosome-enriched fraction (∼ 1ml ...
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bioRxiv - Cell Biology 2023Quote: ... Small EVs were isolated by ultracentrifugation at 120,000 x g and 4°C for 120 min using Optima XE-90 equipment with a SW32Ti rotor (Beckman Coulter). Supernatants were discarded using a pipette ...
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Centriole and transition zone structures in photoreceptor cilia revealed by cryo-electron tomographybioRxiv - Cell Biology 2023Quote: ... OptiPrep step-gradient and centrifuged for 60 min at 19,210 × g at 4 °C in a TLS-55 rotor (Beckman Coulter). The ROS band was collected with an 18G needle ...
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bioRxiv - Cell Biology 2023Quote: ... The gradient was centrifuged at 4 °C and 100,000 ˗ g for 18 h (Optima L-80 XP Ultracentrifuge with Swing Rotor (Beckman Coulter)) ...
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bioRxiv - Neuroscience 2023Quote: ... and samples were centrifuged at 24,000 rpm at 4°C for 1h in a SW41Ti Swinging-Bucket Rotor (Beckman Coulter). After discarding the supernatant ...
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bioRxiv - Immunology 2023Quote: ... virus solutions were clarified of debris by centrifugation at 10,000 rpm at 4°C for 30 minutes in a chilled SW32Ti rotor (Beckman Coulter), followed by pelleting through a 30% cold sucrose solution at 25,000 rpm for 90 minutes ...
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bioRxiv - Microbiology 2023Quote: ... Medium was cleared from EVs by ultracentrifugation at 100,000 ×g for 18 h at 4°C in a LE-80 ultracentrifuge (Beckman Coulter) with a 60Ti rotor [13] ...
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bioRxiv - Biochemistry 2023Quote: ... The diluted extract was then centrifuged at 31,200 rpm for 30 minutes at 4°C in a Type 70 Ti rotor (Beckman Coulter). The supernatant was filtered through a 0.45 µm syringe filter (Millex-GP PES membrane (Millipore SLHP033RS ...
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bioRxiv - Biochemistry 2023Quote: ... The lysate was centrifuged at 40,000 rpm for 60 min at 4°C (XPN-100 ultracentrifuge, Type 45 Ti rotor, Beckman, USA). The supernatant was filtered through a 0.45 μm filter (Pall ...
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bioRxiv - Bioengineering 2023Quote: ... The final supernatant was centrifuged at 100,000g at 4°C for 70 min using an ultracentrifuge (Optima MAX-XP Tabletop Ultracentrifuge, Beckman Coulter). The pellet was either used immediately or stored dry at −80°C ...
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bioRxiv - Biochemistry 2023Quote: ... and the mixtures were then layered under a 20-40% iodixanol discontinuous gradient before to be centrifuged at 260,000 x g for 16h at 4°C using an SW41 Ti rotor (Beckman Coulter). After spinning ...
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bioRxiv - Cancer Biology 2023Quote: ... Filtered supernatants were concentrated by ultracentrifugation at 22.000 rpm for 2h at 4°C with OptimaTM L-90K ultracentrifuge (Beckman Coulter) and stored at −80°C (never refrozen).
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bioRxiv - Cell Biology 2023Quote: ... and pelleted at 100,000xg for 20 min at 4 °C using fixed angle rotor (TLA-100 rotor, Beckman Coulter, 349481) in a benchtop ultracentrifuge (OptimaTL ...
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bioRxiv - Cell Biology 2023Quote: ... Equilibrated tubes were ultra-centrifuged at 100,000xg for 1h at 4°C using a swinging-bucket rotor (SW 32Ti, Beckman Coulter, 369650; Optima XE, Beckman Coulter). Phagosome-containing beads float at the interface between the 10% and 25% layers ...
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bioRxiv - Biophysics 2023Quote: ... The lysate was clarified by centrifugation at 40,000 rpm for 35 min at 4°C (Type 45 Ti Rotor, Beckman Coulter). The resulting supernatant was mixed with 1ml pre-washed IgG Sepharose 6 FastFlow beads (Cytiva ...
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bioRxiv - Biophysics 2023Quote: ... Centrifugation was performed at 154,300 × g for 24 h at 4◦C (SW 41 Ti swinging-bucket rotor, Beckman Coulter). The separated bands were collected dropwise from the bottom of the tube ...
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bioRxiv - Biochemistry 2024Quote: ... The homogenate was clarified by centrifugation for 30 min at 70,560 × g at 4°C in an Optima XPN Ultracentrifuge (Beckman Coulter) using a Ti-45 rotor ...
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bioRxiv - Biochemistry 2022Quote: ... A soluble fraction was obtained after centrifugation of the lysate at 19,000 rpm for 40 min at 4 °C performed using Avanti J-E with a rotor JA-20 (BECKMAN COULTER) to remove insoluble debris.
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Dynamic states of eIF6 and SDS variants modulate interactions with uL14 of the 60S ribosomal subunitbioRxiv - Biochemistry 2022Quote: ... Ribosomes were pelleted by centrifugation at 63,000g for 16–19 hrs at 4°C using a Type 80 Ti rotor (Beckman Coulter). For all ultracentrifugation steps indicated here ...
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Dynamic states of eIF6 and SDS variants modulate interactions with uL14 of the 60S ribosomal subunitbioRxiv - Biochemistry 2022Quote: ... were pelleted by centrifugation at 63,000g for 20 hrs at 4°C in a Type 80 Ti rotor (Beckman Coulter) as described above ...
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bioRxiv - Cell Biology 2022Quote: ... OptiPrep step-gradient and centrifuged for 60 min at 19,210 × g at 4 °C in a TLS-55 rotor (Beckman Coulter). The ROS band was collected with a 18G needle ...
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bioRxiv - Cell Biology 2022Quote: ... The lysate was dounced for 10 strokes on ice and cleared via centrifugation at 80K rpm for 10 minutes at 4°C using a TLA 110 rotor (Beckman) in a tabletop Beckm an ultracentrifuge ...