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Citations for Beckman :
1251 - 1300 of 1814 citations for 1 Benzyl 3 Tert Butyldimethylsilyl Oxy Methyl Piperidin 4 One since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: ... The samples were then centrifuged at 12,000 g for five minutes at 4°C to remove tissue debris with a Microfuge™ 22R centrifuge (Beckman Coulter, Brea, CA). The RNA was extracted using the Invitrogen PureLink™ RNA Mini Kit (Thermo Fisher ...
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bioRxiv - Bioengineering 2021Quote: ... The tubes were then centrifuged at 1000 g for 15 minutes at 4°C using a Microfuge™ 22R (Beckman Coulter, Brea, CA), and the serum was then stored at -80°C ...
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bioRxiv - Microbiology 2021Quote: ... and extracellular vesicles including exosomes and virions were pelleted by ultracentrifugation at 141,000 g at 4 °C for 2 h in a swinging-bucket rotor (SW28; Beckman Coulter, Brea, CA, USA). The pellet was resuspended in filtered PBS and centrifuged at 18,300 g and 4°C for 10 min to remove insoluble aggregates ...
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bioRxiv - Microbiology 2021Quote: ... resuspended in 150 μl 0.1 M HEPES/NAOH pH 7.0 and purified for 48 h (250,000 × g, 4 °C; Beckman Optima LE-80K, rotor SW60-Ti) using a CsCl gradient (0.45 g/ml ...
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bioRxiv - Molecular Biology 2020Quote: ... and centrifuged at 10,000xg for 30 minutes at 4°C in a Beckman Coulter Optima XE-100 (fitted with a SW41Ti Rotor (Beckman Coulter, CA USA)) ...
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bioRxiv - Biophysics 2022Quote: ... EVs were fractionated by OptiPrep density gradient ultracentrifugation (100,000 × g, 18 h, 4 °C) using a SW41 rotor (Beckman Coulter, Fullerton, CA, USA) through a continuous 5–40% OptiPrep (Sigma-Aldrich ...
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bioRxiv - Immunology 2022Quote: ... followed by ultracentrifugation at 110,000 × g for 90 min at 4°C on an Optima XPN -100 ultracentrifuge with SW32Ti rotor (Beckman Coulter, Brea, CA, USA). The isolated EVs was washed with PBS and followed with a secondary ultracentrifugation (110,000 × g for 90 min at 4°C) ...
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bioRxiv - Genomics 2020Quote: ... were layered onto a 1.8M sucrose cushion and ultra-centrifuged at 24,400 rpm at 4°C for 2 hours using a SW28 swinging bucket rotor (Beckman Coulter Optimal centrifuge #L90K). The nuclear pellet was re-suspended in 2% BSA in 1X PBS ...
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bioRxiv - Microbiology 2020Quote: ... EMVs were obtained by ultracentrifugation from 4 mL of filtrate at 100,000 × g (average centrifugal force) and 4°C for 2 h with an OptimaX centrifuge (Beckman Coulter, Brea, CA, USA). The pellets were resuspended in 400 μL of Dulbecco’s phosphate-buffered saline (DPBS ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were spun down at 4°C for 15 minutes at 4000 RPM (3040 RCF) in a J6 centrifuge (Beckman Coulter Life Sciences). Supernatant was discarded and pellets were stored in 15ml conical tubes in -80°C ...
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bioRxiv - Neuroscience 2022Quote: ... The tubes were sealed and centrifuged at 50,000 rpm at 4°C for 2 h in an OptimaTM L-90K ultracentrifuge using the 70.1Ti rotor (Beckman Coulter, Brea, CA, USA). After the centrifugation ...
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bioRxiv - Biochemistry 2020Quote: ... Unbroken cells were pelleted by centrifugation at 18,000 RCF for 30 min at 4°C (Beckman centrifuge J-20-XP, Rotor JA16.250, DE). The supernatant was transferred to 45 Tl rotor (Beckman ...
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bioRxiv - Microbiology 2021Quote: ... Ten milliliters of allantoic or amniotic fluid was centrifuged at maximum speed (16,000 × g) for 30 min at 4 °C using a floor ultra centrifuge (Beckman Optima L-60 Ultracentrifuge). After removal of the supernatant ...
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bioRxiv - Microbiology 2021Quote: ... the SBV samples were purified via sucrose density gradient centrifugation (10–50% range) at 32,500 rpm for 4 h with an SW 41 Ti Swinging-Bucket Rotor (Beckman Coulter Inc., Brea, CA). The SBV band that corresponded to the 40% sucrose gradient was harvested and then centrifuged at 25,000 rpm for 12 h ...
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bioRxiv - Microbiology 2022Quote: ... The supernatant fractions were collected and centrifuged at 75,600 x g for 90 min at 4 °C (Beckman JA25.50 rotor, Beckman Avanati J-25, Beckman Coulter). The resulting pellets were washed again and resuspended in 500 μl potassium phosphate buffer ...
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bioRxiv - Microbiology 2022Quote: ... 100 ml cell cultures were grown over night and harvested by centrifugation at 15,000 x g for 15 min at 4 °C (Beckman JLA-16.250 rotor, Beckman Avanti J-25, Beckman Coulter). Pellets were washed with 20 mM potassium phosphate (pH 7.5 ...
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bioRxiv - Microbiology 2022Quote: ... then clarified by centrifugation at 2000 rpm at 4°C for 5 min in a refrigerated tabletop centrifuge (Beckman Coulter, Indianapolis IN, USA) to remove large debris ...
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bioRxiv - Biochemistry 2023Quote: ... Expression was continued for 7h and the cells harvested by centrifugation at ∼6250g for 15 min at 4°C (Beckman Coulter centrifuge, JLA8.1000).
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bioRxiv - Cell Biology 2024Quote: ... and centrifuged at 16,500 × g (14,500 rpm) at 4°C for 20 min using a Type Ti 45 fixed-angle rotor (Beckman Coulter, k-factor 1.275) to isolate large EVs ...
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bioRxiv - Microbiology 2023Quote: ... cells were pelleted by centrifugation at 6,000 ×g at 4 °C for 15 min with ALLEGRA X-30R (Beckman Coulter, Brea, CA, USA). The supernatant was centrifuged at 15,000 ×g at 4 °C for 15 min with Sorvall LYNX4000 (Thermo Scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... the sample was centrifuged at 268,000 ×g at 4°C for 38 min with an MLA-50 rotor (Beckman Coulter, Brea, CA, USA). The top layer and subsequent 10 fractions (fractions 1–10 ...
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bioRxiv - Plant Biology 2023Quote: ... The disrupted cells were subjected to ultracentrifugation at 4°C and 235000 g for 1h (Beckman Optima™ L-80 XP, rotor Ti45). The resulting supernatant was loaded onto an Äkta purifier system (GE HealthCare ...
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bioRxiv - Cancer Biology 2023Quote: ... the supernatant was ultracentrifuged at 100,000 × g at 4°C for 90 min using Beckman Coulter Optima™ XE-90 Ultracentrifuge (Beckman Colter, Brea, CA) equipped with Type 45 Ti rotor ...
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bioRxiv - Cell Biology 2023Quote: ... the resulting G-actin mix was ultracentrifuged at 90K rpm for 30 minutes at 4°C in an ultracentrifuge (Beckman, Optima TL 100) to remove actin oligomers and/or aggregates.
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bioRxiv - Bioengineering 2023Quote: ... The diluted sample was then centrifuged using a Beckman ultracentrifuge (Optima L90K) with two cycles of 1h10 at 110,000 x g at 4°C (rotor type 70Ti, Beckman Floor Ultracentrifuge Optima L90K). Between them ...
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bioRxiv - Biophysics 2023Quote: ... Virions were banded by density gradient centrifugation at 152,000 x g for 16 h at 4°C in a SW41 rotor (Beckman Coulter SRL, Milan, Italy). The gradient was then fractionated ...
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bioRxiv - Biochemistry 2023Quote: ... expression from the viral backbone and cells harvested between 72h-84h after cell proliferation arrest by centrifugation at 1200g for 15min at 4°C (Beckman Coulter centrifuge, JLA8.1000). Further ...
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bioRxiv - Biochemistry 2023Quote: ... Expression was continued for 16h and the cells harvested by centrifugation at ∼6250g for 15 min at 4°C (Beckman Coulter centrifuge, JLA8.1000). The RING Zn-finger domains from both RING1B and PCGF4 subunits were cloned into a modified polycistronic expression vector (pET29a ...
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bioRxiv - Microbiology 2024Quote: ... The solution was then given a final concentration of 25 mM MgCl2 and ultracentrifuged at 200,000 x g for two hours (4°C) (Beckman Coulter Type 45 Ti). The pellet was then resuspended in 100 μl of distilled water.
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bioRxiv - Microbiology 2024Quote: ... The supernatant was collected and treated with polyethyleneimine to a final concentration of 0.03% incubated on ice for 5 minutes and subsequently centrifuged at 10,000 rpm at 4°C for 10 minutes (Beckman coulter, JA-25.50 rotor). The supernatant was collected and powdered ammonium sulfate (approx 15 g ...
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bioRxiv - Neuroscience 2024Quote: ... cells were harvested and lysed in the lysis buffer described above and ultracentrifuged at 100,000 x g for 30 min at 4 °C using the TLA-110 rotor and the Optima™ MAX-XP tabletop ultracentrifuge (Beckman, Brea, CA). The supernatants were collected as “triton-soluble fraction” and the pellets were washed with the lysis buffer ...
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bioRxiv - Microbiology 2024Quote: ... was added to the supernatant on ice with constant stirring until protein precipitate was observed followed by centrifugation at 10,000 rpm at 4°C for 10 minutes (Beckman coulter, JA-25.50 rotor). The pellet was resuspended in 4 ml of binding buffer (20 mM Tris-HCl ...
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bioRxiv - Plant Biology 2024Quote: ... The supernatant was fractionated at 200 k x g for 20 minutes at 4°C using a Beckman Optima Ultracentrifuge with TLA110 rotor (Beckman Coulter Life Sciences) into a cytosolic (s200 ...
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Scalable Fabrication of a Tough and Recyclable Spore-Bearing Biocomposite Thermoplastic PolyurethanebioRxiv - Bioengineering 2024Quote: ... Spore suspension was centrifuged at 12,000 g at 4 °C for 20 min (Avanti J-E, Beckman Coulter Life Science, Brea, CA, USA). After removing supernatant ...
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bioRxiv - Biochemistry 2024Quote: ... Nucleosome sucrose gradients were then centrifuged at 41,000 rpm and 4°C for 22 h in a SW41 Ti rotor in an Optima L-100 XP ultracentrifuge (Beckman Coulter, Brea, CA). After centrifugation ...
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bioRxiv - Biochemistry 2024Quote: ... samples from different temperatures for each drug treatment were pooled and subjected to ultracentrifugation at 100,000g for 20 minutes at 4°C (Beckman Coulter Optima Max-XP). The soluble proteins from the supernatant were collected for further analysis.
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bioRxiv - Cell Biology 2024Quote: ... The supernatant was subsequently ultracentrifuged at 30,000 × g for 60 min at 4°C in an SW32Ti rotor (Beckman Coulter, Brea, CA, USA) to obtain L-EVs ...
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bioRxiv - Cell Biology 2024Quote: ... Microsomes were enriched at 200 k x g for 20 minutes at 4°C using a Beckman Optima Ultracentrifuge with TLA110 rotor (Beckman Coulter Life Sciences) and washed twice with MIB ...
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bioRxiv - Genomics 2022Quote: ... HIV-1 p24 was stained with Anti-HIV-1 p24-PE (KC57) (Beckman Coulter) for 30 min on ice and washed again 3 times before measuring on the BD FACSCelesta™.
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bioRxiv - Microbiology 2021Quote: ... using anti-HIV-1 p24-PE antibody (#6604667, 1:1000 dilution) purchased from Beckman Coulter ...
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bioRxiv - Developmental Biology 2021Quote: ... cDNA was purified using a 1:1 volumetric ratio of Ampure Beads (Beckman Coulter) and eluted in 20 ml of water ...
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bioRxiv - Genomics 2023Quote: ... The samples were further purified using Ampure XP (1:1) (A63882, Beckman Coulter, US) and diluted in 10 µl elution buffer ...
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bioRxiv - Developmental Biology 2023Quote: ... cDNA was purified using a 1:1 volumetric ratio of Ampure Beads (Beckman Coulter) and eluted in 20 ml of water ...
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bioRxiv - Cancer Biology 2023Quote: ... 7AAD (1:100; Beckman Coulter) was used for dead cell exclusion ...
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bioRxiv - Cancer Biology 2023Quote: ... 7AAD (1:100; Beckman Coulter) was used ...
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bioRxiv - Neuroscience 2020Quote: ... The number and size distribution of the clot fragments that break off the thrombus during thrombectomy are quantified using a Coulter counter (Beckman Multisizer 4, Brea, California). Each removal technique is repeated 8 times for each clot type ...
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bioRxiv - Cell Biology 2020Quote: ... Bacteria were harvested by centrifugation at 4000 rpm for 25 min at 4°C in a Beckman J6M clinical centrifuge (Beckman Coulter Diagnostics; Brea, California), and re-suspended on ice in lysis buffer (50 mM Tris pH 7.5 ...
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bioRxiv - Microbiology 2020Quote: ... and layered on top of a two-step sucrose gradient (20%/50% w/v in HNE) and centrifuged for 2 h at 154 000 x g at 4 °C in a SW41 rotor (Beckman Coulter, Woerden, the Netherlands) to clear from cell debris ...
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bioRxiv - Neuroscience 2022Quote: ... The samples were then spun at 31000 x g (18468 rpm) for 5 min at 4°C in a fixed-angle rotor (50.2 Ti rotor in Beckman Coulter Optima L-100 XP) with slow brake ...
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bioRxiv - Plant Biology 2024Quote: ... The size of the cell population and the number of intact cells were measured using a Coulter Counter (Multisizer 4; Beckman Counter, Brea, CA, USA).