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Citations for Beckman :
1201 - 1250 of 1427 citations for 7 Boc 3 chloro 5 6 7 8 tetrahydroimidazo 1 2 a pyrazine 2 carboxylic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... mTurquoisehigh cells were sorted into 96-well plates at 1 cell/well using a MoFlo Cell Sorter (Beckman Coulter). For cells transfected with sgRNA/Puro plasmids ...
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bioRxiv - Developmental Biology 2023Quote: ... Double size selection to remove primer dimers and fragments exceeding 1 kb was performed using SPRIselect beads (Beckman Coulter). Another quality control was performed with High Sensitivity DNA assay using the Fragment Analyzer (Agilent) ...
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bioRxiv - Molecular Biology 2024Quote: ... for ultracentrifugation at 100,000 × g for 1 hour at 4°C (JXN-30 centrifuge, JA-30.50 Ti Rotor, Beckman Coulter). The pellet was washed with PBS and centrifuged again at 100,000 × g for 1 hour at 4°C to remove soluble contaminant ...
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bioRxiv - Molecular Biology 2024Quote: ... and 1 M sucrose) at 100,000 rpm (543,000 × g) and 4°C for 1 h with a TLA110 rotor (Beckman Coulter) and an Optima MAX-TL Ultracentrifuge (Beckman Coulter) ...
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bioRxiv - Developmental Biology 2023Quote: ... Size selection and purification was achieved by double-sided (0.65 × −1× volume) using SPRIselect magnetic beads (Beckman Coulter, B23318). Concentrations were measured using the Qubit HS dsDNA assay ...
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Small Molecule Screen Identifies Pyrimethamine as an Inhibitor of NRF2-driven Esophageal HyperplasiabioRxiv - Cancer Biology 2022Quote: ... Individual compounds (1 µM in 0.1% DMSO) were added to each well using a Biomek NX workstation (Beckman-Coulter)(1 well per compound) ...
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bioRxiv - Cell Biology 2023Quote: ... and 1 mM PMSF) and centrifuged for 15 hours at 4°C with 74,400 xg (SW 32.1 Ti; Beckman Coulter). 24 equal volume fractions were collected from the top and subjected to TCA precipitation ...
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bioRxiv - Plant Biology 2023Quote: ... It was then centrifuged again at 100,000 x g for 1 h at 4 ⁰C (TLA 55 rotor, Beckman Coulter ...
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bioRxiv - Biophysics 2023Quote: ... The supernatant was subjected to a second round of ultracentrifugation at 100,000 x g for 1 hour at 4°C in a Type 45Ti rotor (Beckman) to pellet cell membranes ...
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bioRxiv - Plant Biology 2023Quote: ... The tubes were then centrifuged at 100,000 x g for 1 h at 4 ⁰C (TLA 55 rotor, Beckman Coulter ...
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bioRxiv - Immunology 2023Quote: ... SSM liquid culture was incubated for 24 hours at 36°C with constant shaking at 180 rpm until reaching mid-log phase OD600nm 0.5 with 1 cm path width (Beckman Coulter™ DU 530 UV Vis spectrophotometer) ...
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Comparative membrane proteomics reveals diverse cell regulators concentrated at the nuclear envelopebioRxiv - Cell Biology 2023Quote: ... The gradients then were centrifuged at 35,000 rpm (210,000g) for 1 h at 4°C with no brake in an SW41Ti rotor (Beckman Coulter). Two samples were collected from the nucleus gradient ...
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bioRxiv - Neuroscience 2023Quote: ... the supernatant was collected and applied for further centrifugation at 40,000 rpm for 1 h at 4°C (45 Ti fixed-angle rotor, Beckman) to pellet the membranes ...
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bioRxiv - Evolutionary Biology 2024Quote: ... DNA was enriched in samples with low DNA concentration (1-60 ng/μl) using AMPureXP Beads (SPRIselect, Beckman Coulter) in a 1:1 ratio ...
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bioRxiv - Neuroscience 2023Quote: ... NP-40 prepared in DPBS] and then ultracentrifuged at 100,000x g for 1 h at 4 °C using a TLA-55 rotor (Beckman). The supernatant was removed ...
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bioRxiv - Biochemistry 2023Quote: ... The mixture was incubated for 30 min at 4 °C and centrifuged at 100,000 × g at 4 °C for 1 h using a TLA100 fixed angle rotor (Beckman). The supernatant fraction was carefully removed ...
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bioRxiv - Developmental Biology 2024Quote: ... in 1×MMR final were manually prepared in pre-chilled 14 mL ultra-clear centrifuge tubes (Beckman Coulter, #344060) in the following order from the bottom to the top of the tube ...
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bioRxiv - Genomics 2024Quote: ... They were transduced via spinfection at 1000 rpm for 1 hour at 30 °C (Beckman Coulter, Allegra X-14R), followed by overnight incubation ...
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Syntaxin-2 inhibits excessive phagocytic uptake but promotes phagolysosomal clearance in macrophagesbioRxiv - Cell Biology 2024Quote: ... The samples were then centrifuged at 100,000 g for 1 hr at 4°C using a SW40Ti rotor (Beckman). Phagosomes were recovered from the interface of 10 and 25% sucrose (Fig ...
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bioRxiv - Plant Biology 2024Quote: ... the supernatant after the 40,000 g spin was transferred to ultracentrifuge tubes and centrifuged at 100,000 g for 1 h at 4 °C (TLA100.3 rotor, Optima TLX Ultracentrifuge; Beckman Coulter). P40 and P100-P40 pellets were resuspended in cold and filtered VIB or 100 mM Tris pH 7.4 and either used immediately or stored at –80 °C until further use.
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bioRxiv - Plant Biology 2024Quote: ... tubes and centrifuged at 40,000 g for 1 h at 4 °C (TLA100.3 rotor, Optima TLX Ultracentrifuge; Beckman Coulter). Where indicated ...
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bioRxiv - Immunology 2024Quote: ... Cells were washed and resuspended in PBS 1% FBS 2mM EDTA and run on a Cytoflex LX (Beckman Coulter).
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bioRxiv - Microbiology 2024Quote: ... For p24 staining (RD1-conjugated mouse anti-p24 clone KC57, cat# 6604667, 1:100 dilution, Beckman Coulter, Brea, CA), cells were fixed and permeabilized using the Cytofix/Cytoperm kit (BD Biosciences ...
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bioRxiv - Microbiology 2024Quote: ... Amplicons were visualized on a 1% agarose gel and purified with Agencourt AMPure XP beads (Beckman Coulter, Brea, CA). The concentration of purified amplicons was quantified using the Qubit High Sensitivity dsDNA kit and a Qubit Fluorometer (Invitrogen) ...
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bioRxiv - Biophysics 2020Quote: ... Very small amounts of fibrillar species formed during this time were removed by ultracentrifugation for 1 h at 90000 rpm (Optima TLX Ultracentrifuge, Beckman Coulter ...
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bioRxiv - Cell Biology 2020Quote: ... Crude mitochondria were loaded on top of the chilled gradient and separated at 100,000 Å∼ g for 1 h at 4°C (SW41 rotor; Beckman). Intact purified mitochondria were recovered from a brown band at around 16% Histodenz™ concentration ...
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bioRxiv - Neuroscience 2022Quote: ... The amount of p24 capsid protein was determined by the HIV-1 p24 ELISA antigen assay (Beckman Coulter, Fullerton, CA). Virus from different productions averaged 175 ng/µl of p24 antigen.
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bioRxiv - Microbiology 2019Quote: ... and centrifuged at 40,008 × g for 1 hour at 4ºC in an Beckman L-100 XP ultracentrifuge with the SW 41 Ti swing rotor (Beckman Coulter). Following centrifugation ...
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bioRxiv - Biochemistry 2019Quote: ... the cells were dissolved in the same buffer as for TtBac-WT 1-123 and spun for 30 min at 9k rpm in a Ti45 rotor (Beckman). The pellet was dissolved in the same buffer and from here onwards the same protocol as for TtBac-WT 1-123 was used.
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bioRxiv - Molecular Biology 2020Quote: ... The total bacterial lysate was centrifuged at 48,384 x g at 4 °C for 1 hr (Beckman JA-25.50 rotor), and the supernatant was applied to a 5 mL HisPur Ni-NTA resin (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... actins were cleared by highspeed centrifugation at 316,613 x g for 1 hour at 4°C in a TLA100 rotor (Beckman Coulter). Only the top 80% of the supernatant was collected to perform TIRF microscopy ...
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bioRxiv - Bioengineering 2021Quote: ... Cultures were grown in a MTP-48-BOH 1 FlowerPlate in a BioLector Pro (both Beckman Coulter Life Sciences, USA) at 1400 rpm ...
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bioRxiv - Biochemistry 2020Quote: ... The eluted sample was collected by centrifugation at 2000 rpm for 1 minutes 4 °C in a F241.5P rotor using a 149 Microfuge 22R centrifuge (Beckman Coulter). One aliquot of the eluted sample was resolved on SDS-PAGE ...
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bioRxiv - Biochemistry 2020Quote: ... After increasing the NH4Cl concentration of the supernatant to 1 M to dissociate bS1 from 70S ribosomes the sample was centrifuged in a Type 70 Ti rotor (Beckman) at 60,000 g for 2 hours ...
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bioRxiv - Systems Biology 2020Quote: ... GFP productivity was determined by measuring the GFP signal (FL-1 channel) of cells by flow cytometry (Gallios, Beckman Coulter).
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bioRxiv - Microbiology 2021Quote: ... and stained intracellularly using PE-conjugated mouse anti-p24 mAb (clone KC57; Beckman Coulter, Brea, CA, USA; 1:100 dilution) in combination with Nef or Vpu rabbit antisera (1:1000 dilution) ...
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bioRxiv - Genomics 2021Quote: ... 1 mM EDTA (corresponding to a refractive index of 1.3700) and loaded into OptiSeal polypropylene centrifuge tubes (Beckman Coulter, 362185). Centrifugation was performed in a near-vertical NVT-90 rotor (Beckman Coulter ...
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kinesin recruitment by adapter SKIP on melanosomes is dynamically controlled by LC3B phosphorylationbioRxiv - Cell Biology 2021Quote: ... The post nuclear supernatant was collected and separated on a stepwise sucrose density gradient and centrifuged at 100,000 g for 1 hour at 4°C in a swing-out rotor (Beckman coulter). The sucrose density gradients comprised 0.25 ...
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bioRxiv - Molecular Biology 2022Quote: ... Stained cells were resuspended in sort buffer at 1 × 106/ml for fluorescence analysis on a Cytoflex S cell analyser (Beckman). SARS-CoV-2 Spike variants were generated by site-directed mutagenesis with Q5 polymerase (New England Biolabs ...
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bioRxiv - Biochemistry 2022Quote: ... and the debris and membranes were removed by ultracentrifugation at 205,000 g for 1 hr at 4 °C (rotor 45 Ti, Beckman Coulter). The supernatants were applied to IMAC with Ni2+-NTA-agarose resin (Qiagen ...
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bioRxiv - Molecular Biology 2022Quote: ... The sucrose gradient was centrifuged at 24,400 rcf for 1 h at 4°C in a SW41Ti Swinging-Bucket Rotor (Beckman Coulter), and nuclei at the bottom of the gradient were resuspended in PBS ...
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bioRxiv - Plant Biology 2021Quote: ... The thylakoid membranes with different densities were analytically separated at 125,000 × g and 4°C for 1 h (SW 41 Ti, Beckman Coulter).
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bioRxiv - Microbiology 2021Quote: ... culture samples were diluted (1:100 or 1:1000) with 0.2-µm-filtered 18% BSW and were analysed with a Multisizer M4 Coulter cytometer (Beckman-Coulter) equipped with a 20 or 30 µm aperture tube ...
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bioRxiv - Microbiology 2020Quote: ... OBs were loaded on to a discontinuous 35–60% (w/w) sucrose gradient and centrifuged at 20000 rpm and 4°C for 1 hour in a SW41 rotor (Beckman). The band containing the OBs was removed ...
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bioRxiv - Cell Biology 2020Quote: ... After purification of the PCR product with a 1: 0.6 ratio of PCR product to AMPure XP beads (Beckman Coulter), the success of cDNA preparation was confirmed using a 2100 Bioanalyzer with High-sensitivity DNA chip (Agilent) ...
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bioRxiv - Neuroscience 2020Quote: ... The remaining supernatant (conditioned medium) was subjected to ultracentrifugation (UC) (100,000 x g 1 h) using rotors Ti45 or SW32Ti (Beckman Coulter). The pellet was rinsed with PBS and spun again using rotor SW55Ti (100,000 x g 1 h).
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bioRxiv - Neuroscience 2022Quote: ... The remaining supernatant (conditioned medium) was subjected to ultracentrifugation (UC) (100,000 x g, 1 h) using rotors Ti45 or SW32Ti (Beckman Coulter). The pellet was rinsed with PBS and spun again using rotor SW55Ti (100,000 x g ...
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bioRxiv - Neuroscience 2022Quote: ... 10 mM Tris-HCl) and centrifugated at 24,000 rpm for 1 h at 4°C in a SW41Ti Swinging-Bucket Rotor (Beckman Coulter). Supernatant was discarded and nuclei pellets were re-suspended in 800 µL of PBS ...
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bioRxiv - Biochemistry 2019Quote: ... 10,000 events were then immediately analyzed from 1 of the 4 150 µL aliquots using a CytoFLEX benchtop cytometer (Beckman). All subsequent chase time points were collected using this initial analysis as a reference ...
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bioRxiv - Biochemistry 2019Quote: ... The supernatant was then slowly mixed with an equal volume of 4 M ammonium sulphate (2 M final concentration) at 4°C for 1 hour and spun at 10 K rpm using a JA-17 rotor (Beckman). Pellets containing RAD51 were suspended in 25 ml of resuspension buffer (0.5 M KCl ...