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Citations for Beckman :
1201 - 1250 of 2271 citations for 5R 3 4 5 6 Tetrahydro 5 phenyl N benzyloxycarbonyl 4 H 1 4 oxazin 2 one since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... 5 ml of this mix was transferred to a 17-ml ultracentrifuge tube (Beckman) and overlayed with the following sucrose solutions ...
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bioRxiv - Microbiology 2024Quote: ... and mixed with 5 mL of liquid scintillation cocktail (Beckman Coulter # 141349, Brea, CA) before measuring the radioactivity (Counts per minute [CPM] ...
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bioRxiv - Biochemistry 2020Quote: ... The functional complex was recovered as a jelly-like pellet by centrifugation at 60,000 rpm at 25°C for 2 h in an ultracentrifuge (128,000 g in a TLA 120.2 Beckman rotor) and packed into a 1.9 mm rotor ...
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bioRxiv - Microbiology 2023Quote: ... The filtrate was subjected to ultracentrifugation at 200,000 xg for 2 h (Optima L-100 XP ultracentrifuge; Beckman Coulter). Supernatants were discarded ...
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bioRxiv - Neuroscience 2023Quote: ... PI (1 μl/100 μl) was added 5 minutes before starting the reading on the flow cytometer CytoFlex S (Beckman Coulter, Brea, CA, USA). Results were shown and analyzed through CytExpert flow cytometry analysis software.
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bioRxiv - Molecular Biology 2022Quote: ... Stained cells and fluorescence minus one controls were then resuspended in SB + 1:1000 DNase + 1:1000 Draq7 (Beckman Coulter) as a live/dead stain ...
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bioRxiv - Biophysics 2022Quote: ... The fractions containing monomeric peptide were pooled and centrifuged at 386000 g for 3 h in an Optima TLX ultracentrifuge equipped with a TLA 120.1 rotor (Beckman Coulter, Atlanta, GA, USA). Orthophosphoric acid or sodium phosphate were added to a final concentration of 20 mM to adjust the pH to 2.8 or 7.4 ...
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bioRxiv - Bioengineering 2023Quote: ... the supernatant was then taken and loaded into a 10% to 45% sucrose gradient solution and centrifuged at 360000 rpm for 3 h (Beckman Optima XE-100). 18 fractions from each sample were collected using a piston gradient fractionator (Biocomp ...
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bioRxiv - Biochemistry 2020Quote: ... The supernatant was centrifuged at 40,000 rpm for 1 h in Ti45 rotor (Beckman) to harvest cell membrane in pellets ...
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bioRxiv - Cell Biology 2020Quote: ... Exosomes were then pelleted (TLA-100 rotor Beckman, 1 h at 90 000 rpm). Supernatants and pellets were then loaded on 12% SDS-PAGE and the indicated proteins were analyzed by Western blot.
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bioRxiv - Immunology 2020Quote: ... and ultracentrifuged at 40,000 RPM for 1 h (45 Ti fixed-angle rotor, Beckman). The soluble proteome was incubated with pre-equilibrated anti-FLAG M2 affinity gel (Sigma ...
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bioRxiv - Cell Biology 2022Quote: ... After a 1 h spin at 55,000 rpm (TLS-55 rotor; Beckman-Coulter, USA) at 4°C ...
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bioRxiv - Biochemistry 2024Quote: ... The supernatant was centrifuged at 40,000 rpm for 1 h in Ti45 rotor (Beckman). Membrane pellets were harvested ...
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bioRxiv - Biochemistry 2021Quote: ... Equal parts of 30% and 10% iodixanol gradient solutions were layered on the mitochondrial fraction and then samples were centrifuged at 50,000 x g for 2 h (Beckman SW41TI). The mitochondrial layer was collected ...
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bioRxiv - Cell Biology 2021Quote: Microtubules were sedimented onto poly-L-lysine coated coverslips by centrifuging through 20% glycerol BRB80 cushion at 4700 rpm for 2 h in S6096 rotor (Beckman). Coverslips were subsequently fixed with 20°C methanol ...
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bioRxiv - Microbiology 2021Quote: ... pspAX.2 and oligonucleotides were incubated for 48 h and GFP expression level was analyzed by CytoFLEX flow cytometer (Beckman).
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bioRxiv - Bioengineering 2022Quote: ... the conditioned media was subjected to an additional centrifugation step of 118,000 × g for 2 h in a Type 70 Ti ultracentrifuge rotor (Beckman Coulter). The resulting EV pellet was resuspended in 1X PBS and placed into a Nanosep 300 kDa MWCO spin column (Pall ...
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bioRxiv - Biophysics 2023Quote: ... the supernatant of cell lysates was centrifuged to isolate the total membrane fraction at 24,000 rpm for 2 h in the 45Ti rotor using ultracentrifuge (Beckman-Coulter). Membrane resuspension in 50 mM Tris-HCl buffer (pH 8.0 ...
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bioRxiv - Neuroscience 2023Quote: ... This supernatant was next subjected to ultracentrifugation at 120,000 x g for 2 h at 4LJ in a SW 41 Ti Rotor Swinging-Bucket rotor (Beckman Coulter), and the pellets collected as small factors like exosome ...
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bioRxiv - Neuroscience 2023Quote: ... Fraction LP2 was obtained after centrifugation of fraction LS1 for 2 h at 165,000 g in a SW40 Ti rotor (Beckman, US). Corresponding supernatant was collected as Fraction LS2 ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were ultracentrifuged at 4 °C and 150,000 ˗ g for 2 h (Optima L-80 XP Ultracentrifuge with Type 70.1 Ti Fixed-Angle Titanium Rotor (Beckman Coulter)) and supernatants were removed ...
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bioRxiv - Biochemistry 2024Quote: ... The supernatant containing the membrane fraction was transferred to Beckman ultracentrifuge tubes and subjected to ultracentrifugation for 2 h at 41,000 r.p.m (Beckman Optima L-100 XP ultracentrifuge, Beckman 45 Ti rotor). Membrane pellets were resuspended to a total protein concentration of 3.5 mg/mL in ice cold 1 x PBS using the Bicinchoninic Acid (BCA ...
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bioRxiv - Immunology 2024Quote: ... and EVs were isolated by ultracentrifugation (UC) at 100,000 x g for 2 h using a SW40 Ti rotor (Beckman Coulter) at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... Sucrose density gradients (5%–45% w/v) were freshly made in SW41 ultracentrifuge tubes (Beckman) using a Gradient Master (BioComp Instruments ...
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bioRxiv - Cancer Biology 2020Quote: ... 5 mM MgCl2) and centrifuged at 45,000 rpm (SW 55 Ti rotor, Beckman Coulter, Inc.) (adapted from Liang et al ...
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bioRxiv - Microbiology 2021Quote: ... Product were PCR amplified by 5 cycles and cleaned up using SPRI beads (Beckman Coulter) and subjected to paired-end 300bp sequencing on Illumina Miseq ...
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bioRxiv - Genomics 2024Quote: ... After 5 days cells were harvested for genomic DNA purification using Agencourt DNAdvance (Beckman Coulter). Transfection of human primary hepatocytes were performed using Lipofectamine MessengerMAX Transfection Reagent (Thermo Fisher) ...
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bioRxiv - Biophysics 2024Quote: ... the microtubules were centrifuged for 5 min at 22,000 RPM (TLA100.0, Beckman Optima MAX-XP) at 37°C ...
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bioRxiv - Biochemistry 2023Quote: ... Ultracentrifugation was carried out for 5 hours at 133,000xg on a SW60-Ti (Beckman Coulter) rotor to resolve each gradient ...
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bioRxiv - Cancer Biology 2023Quote: ... After 5-7 days cells were counted using a Z1 Coulter Particle Counter (Beckman Coulter).
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bioRxiv - Microbiology 2023Quote: ... pH 7.4) and loaded into a centrifuge tube (Beckman Cat. No. 344057, 5 ml capacity). One milliliter of 20% sucrose in 20 mM HEPES pH 7.4 ...
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bioRxiv - Immunology 2024Quote: ... and BA.5 RBD were sorted using the MoFlo Astrios EQ Cell Sorter (Beckman Coulter). The data from FACS were gathered using Summit 6.0 and analyzed with FlowJo software version 10.8.
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bioRxiv - Immunology 2021Quote: ... supplemented with protease inhibitor cocktail and 1 mM TCEP and centrifuged at 40,000 RPM for 1 h (45 Ti fixed-angle rotor, Beckman). Supernatant was incubated with amylose beads pre-equilibrated in the same buffer for 1 h at 4 °C and loaded onto a gravity flow column ...
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bioRxiv - Microbiology 2022Quote: ... filtered supernatant was underlaid with 20% sucrose PBS using a pipetting needle and centrifuged at 39 000 rpm for 2 h using an SW-41 rotor (Beckman Coulter). The supernatant and sucrose solution were carefully removed ...
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bioRxiv - Microbiology 2022Quote: ... The virus suspension was pelleted through a 40% sucrose cushion by centrifugation for 2 h at 40,000 rpm in a SW55 Ti rotor (Beckman, Fullerton, CA) at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... Samples were incubated for 2 h at 16°C and double stranded cDNA was purified using AMPure XP beads (Beckman Coulter). For testing transcription activity of predicted genes ...
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bioRxiv - Genetics 2020Quote: ... Reverse crosslinking reactions were incubated 2 h at 65°C and cleaned using Agencourt Ampure XP SPRI beads (Cat #A63880, Beckman Coulter) with a 2x bead:sample ratio ...
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bioRxiv - Biochemistry 2024Quote: ... 50 mM Tris-HCl pH 7.4) and ultracentrifuged at 250 000 x g for 2 h (Beckman Optima TL 100 Ultracentrifuge). After ultracentrifugation ...
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bioRxiv - Microbiology 2023Quote: ... viruses were collected and concentrated by ultracentrifugation (37,000 rpm, 2 h 30, 15 °C; Beckman Type 45 Ti fixed-angle rotor). The virus titer was determined using a plaque assay.
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bioRxiv - Biophysics 2023Quote: ... and 12-fold diluted in ice-cold SCL and subjected to ultracentrifugation at 100 000 x gav for 2 h in a Ti 70 fixed-angle rotor (Beckman Coulter). The resulting pellets were resuspended in SCL and freshly used for downstream analysis and never subjected to freezing and thawing.
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bioRxiv - Cancer Biology 2024Quote: ... The exosome pellet was resuspended in 35 ml of 0.2 μm-filtered 1x PBS and collected by ultracentrifugation at 100,000 × g for 2 h (45ti rotor, Optima L-100 XP Centrifuge, Beckman Coulter, USA). Exosome pellets were resuspended in 200-300 µL of 0.2 μm-filtered 1x PBS and stored at -80°C ...
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bioRxiv - Cell Biology 2020Quote: ... liposomes were centrifuged at 100,000 g for 1 h using a Sw55 rotor (Beckman Coulter), supernatant was collected and stored at 4°C up to two weeks under argon.
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bioRxiv - Cell Biology 2020Quote: ... for 1 h at 37°C before purification with 42 μl AMPure beads (Beckman Coulter). The sample was eluted in 20 μl water which served as input for sequencing library generation ...
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bioRxiv - Microbiology 2022Quote: ... ultracentrifugation was performed at 100,000 g for 1 h (Optima L-90K ultracentrifuge; Beckman Coulter). The supernatant was stored at -20°C ...
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bioRxiv - Neuroscience 2024Quote: ... the cell lysate underwent centrifugation at 12,000 × g for 1 h (JA 25.50, Beckman Coulter) and supernatant was loaded to anti-Flag M2 affinity resin (GenScript) ...
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bioRxiv - Microbiology 2023Quote: ... The lysate was cleared at 100×kg for 1 h in Ti70 rotor (Beckman Coulter). The pellet was subsequently resuspended in lysis buffer with 8 M Urea and 20 mM imidazole at room-temperature for 1 h ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 mM DTT) and centrifuged for 13 h in a SW40 Ti rotor (Beckman Coulter) at 19,200 rpm ...
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bioRxiv - Biochemistry 2024Quote: ... and cleared by centrifugation for 1 h at 35,000 rpm in a Ti45 rotor (Beckman).
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bioRxiv - Immunology 2021Quote: ... The tubes were centrifuged at 10,000 g for 5 min (Microfuge 16, Beckman Coulter, Indianapolis, IN) and the collected media was combined with the previously pipetted media.
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bioRxiv - Biophysics 2022Quote: ... microtubules were spun down at 200,000 × g for 5 min using an Airfuge ultracentrifuge (Beckman-Coulter) to exclude free GTP ...