Labshake search
Citations for Beckman :
1151 - 1200 of 1841 citations for 1 tert Butoxy 4 methyl 2 nitrobenzene since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2024Quote: ... The fluid was centrifuged at 1,000 x g for 10 minutes at 4 C using an Allegra X-30R centrifuge (Beckman Coulter Life Sciences). The resulting supernatant was centrifuged at 200,000 x g for 30 minutes at 4 °C using a Beckman Optima Ultracentrifuge with TLA110 rotor (Beckman Coulter Life Sciences) ...
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bioRxiv - Plant Biology 2024Quote: ... The resulting supernatant was centrifuged at 200,000 x g for 30 minutes at 4 °C using a Beckman Optima Ultracentrifuge with TLA110 rotor (Beckman Coulter Life Sciences). The pellet was resuspended in 30 µL of cold ddH20 and stored in −80 °C until further use.
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bioRxiv - Biochemistry 2024Quote: ... Purified nucleosome arrays were subsequently isolated on 5-40% w/v sucrose gradients in 0.5X TE by centrifuging the gradients at 39,000 rpm and 4°C for 8 h in a SW41 Ti rotor (Beckman Coulter, Brea, CA). After centrifugation ...
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bioRxiv - Cell Biology 2024Quote: ... The crude pellets from MSC-CM and HDF-CM after the first-round 100,000 × g ultracentrifugation were washed with PBS at 100,000 × g and 4 ℃ for 45 min using Optima™ MAX-TL Ultracentrifuge (Beckman Coulter, USA) and TLA-120.2 Rotor (Beckman Coulter ...
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bioRxiv - Genetics 2021Quote: ... and samples were evaluated 2 minutes later with a Cytomics FC500 flow cytometer (Beckman Coulter, Brea, CA, USA) AO was excited with a 488 nm argon laser ...
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bioRxiv - Genomics 2020Quote: ... The PCR products were purified by using 1.3X Agencourt AMPure XP beads (2 or 3 cycles; Beckman Coulter) and evaluated by using an Agilent High Sensitivity DNA Kit on an Agilent 2100 Bioanalyzer (Agilent) ...
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bioRxiv - Cancer Biology 2020Quote: ... concentrated for 2 h by ultracentrifugation in a swing-out rotor SW-32Ti (Beckman Coulter, Brea, US-CA) at 26000 g ...
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bioRxiv - Microbiology 2023Quote: ... The filtrate was subjected to ultracentrifugation at 200,000xg for 2 h (Optima L-100 XP ultracentrifuge; Beckman Coulter). Supernatants were discarded ...
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bioRxiv - Microbiology 2024Quote: ... (The filtrate was subjected to ultracentrifugation at 100,000g for 2 h (Optima L-90 K ultracentrifuge; Beckman Coulter). The supernatant was discarded and the pellet was washed with sterile PBS and filtered through a sterile 0.2 μm-pore polyvinylidene difluoride (PVDF ...
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bioRxiv - Cell Biology 2022Quote: ... Lentiviral particles were then concentrated by centrifugation for 2 hours at 20,000 rcf (Beckman Coulter, Optima L-100K) in round bottom polypropylene tubes (Beckman Coulter ...
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bioRxiv - Cell Biology 2022Quote: Total RNA of Caco-2 cells and human ileum organoids was extracted using RNAdvance Tissue kit (Beckman Coulter) and total RNA of mouse organoids was isolated using Quick-RNA Miniprep Plus Kit (Zymo Research) ...
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bioRxiv - Physiology 2024Quote: ... was analysed via a sequential 2-step immune-enzymatic assay (inter-assay CV 5.2-7.8%) according to manufacturer’s instructions (Beckman Coulter) and follicle stimulating hormone (FSH ...
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bioRxiv - Immunology 2024Quote: ... The whole cell lysate was sonicated for 2 min and the cell lysate was centrifuged for 60 min at 20,000 rpm (JA 25.50 rotor, Beckman). The recombinant P72 protein in the supernatant were collected and applied to the anti-HA Magnetic Beads (MCE ...
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bioRxiv - Cancer Biology 2024Quote: ... 2 μl of the sample pre- and post-bead clean-up (SPRIselect beads, Beckman Coulter, cat. no. B23317) were run in the TapeStation D1000 as a quality control to check peak size ...
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bioRxiv - Cell Biology 2024Quote: ... MKs were subsequently fixed with 2% paraformaldehyde and activation measured using the CytoFLEX flow cytometer apparatus (Beckman Coulter). Normalized mean florescence intensity (MFI ...
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bioRxiv - Genomics 2021Quote: ... then re-purified with SPRI beads with 1:1 ratio (Beckman Coulter, B23317), and eluted in 25 µl of water (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... by ultra-centrifugation in a Beckman L7-65 ultracentrifuge at 25,000 rpm for two hours at 4°C using a Beckman SW28 rotor (Beckman Coulter, Brea, CA, USA). Supernatants were aspirated off and the pellets were re-suspended in PBS (pH 7.4) ...
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bioRxiv - Microbiology 2020Quote: ... by centrifugation in a Beckman L7-65 ultracentrifuge at 25,000 rpm for 2h at 4°C using a Beckman SW28 rotor (Beckman Coulter, Brea, CA, USA). Supernatants were aspirated and the pellets were re-suspended in PBS (pH 7.4) ...
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bioRxiv - Molecular Biology 2021Quote: ... The centrifugation is performed on a SW60Ti rotor at 40 000 g for 22 minutes at 4°C (Beckman Optima L-90K Ultracentrifuge). The mitochondrial fraction (400µL ...
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bioRxiv - Molecular Biology 2020Quote: ... and plasma was separated by centrifugation (2,000 × g, 20 min, 4 °C) in a Microfuge 20R (cat. no. B30149, Beckman Coulter, Brea, CA, USA). Plasma was transferred to new 1.5 mL microcentrifuge tubes and further clarified by centrifugation (10,000 × g ...
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bioRxiv - Biophysics 2022Quote: ... shortly sonified and centrifuged for 30 min at 100 000 g at 4°C (Centrifuge Beckman Coulter Optima XPN-80, Rotor type 70.1).) ...
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bioRxiv - Biophysics 2022Quote: ... The samples were centrifuged for 30 min at 100 000 g at 4°C (Centrifuge Beckman Coulter Optima XPN-80, Rotor type 70.1). The supernatant ...
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bioRxiv - Microbiology 2020Quote: ... CLP assembly reactions were incubated at 25°C for 30 min before loading onto 15-30% (wt/wt) sucrose gradients and spinning at 159,599 X g for 43 min at 4°C (Beckman Coulter TLS 55 rotor). Gradients were immediately fractionated and aliquots of each fraction were denatured by boiling in 1% SDS to ensure antibody access ...
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bioRxiv - Biochemistry 2020Quote: ... Unbroken cells were pelleted by centrifugation at 18,000 RCF for 30 min at 4°C (Beckman centrifuge J-20-XP, Rotor JA16.250, DE). The supernatant was transferred to 45 TI rotor (Beckman ...
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bioRxiv - Bioengineering 2020Quote: ... and the supernatant was centrifuged again at 26000 × g at 4 °C for 15 min using an Optima L-90K (Beckman Coulter, Brea, CA) ultracentrifuge with a 70-Ti fixed angle rotor (Beckman Coulter) ...
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bioRxiv - Biochemistry 2021Quote: ... supernatants were collected and centrifuged with 150.000 x g (at rav) for 30 min at 4 °C using an Optima™ MAX-XP Ultracentrifuge (Beckman Coulter; TLA120.2 rotor). Pellets were resuspended in PBS and 4 µg of primary antibody were added for 30 min ...
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bioRxiv - Genomics 2021Quote: ... The spores were washed twice with sterile ddH2O and pelleted at 3700g for 5 min at 4 °C in an Allegra X-12R centrifuge (Beckman, Brea, CA, USA). Spores were resuspended in sterile ddH2O and kept at 4°C until experiments.
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bioRxiv - Neuroscience 2021Quote: ... The resulting lysates were centrifuged at 100,000Xg for 30-60 min at 4°C in an Optima TLX tabletop ultracentrifuge using a TLA-110 rotor (Beckman Coulter, Fullerton, CA). The clarified supernatants were removed and transferred into the new tubes ...
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bioRxiv - Biochemistry 2020Quote: ... Unbroken cells were pelleted by centrifugation at 18,000 RCF for 30 min at 4°C (Beckman centrifuge J-20-XP, Rotor JA16.250, DE). The supernatant was transferred to 45 TI rotor (Beckman ...
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bioRxiv - Bioengineering 2021Quote: ... The samples were then centrifuged at 12,000 g for five minutes at 4°C to remove tissue debris with a Microfuge™ 22R centrifuge (Beckman Coulter, Brea, CA). The RNA was extracted using the Invitrogen PureLink™ RNA Mini Kit (Thermo Fisher ...
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bioRxiv - Bioengineering 2021Quote: ... The tubes were then centrifuged at 1000 g for 15 minutes at 4°C using a Microfuge™ 22R (Beckman Coulter, Brea, CA), and the serum was then stored at -80°C ...
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bioRxiv - Microbiology 2021Quote: ... resuspended in 150 μl 0.1 M HEPES/NAOH pH 7.0 and purified for 48 h (250,000 × g, 4 °C; Beckman Optima LE-80K, rotor SW60-Ti) using a CsCl gradient (0.45 g/ml ...
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bioRxiv - Molecular Biology 2020Quote: ... and centrifuged at 10,000xg for 30 minutes at 4°C in a Beckman Coulter Optima XE-100 (fitted with a SW41Ti Rotor (Beckman Coulter, CA USA)) ...
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bioRxiv - Biophysics 2022Quote: ... EVs were fractionated by OptiPrep density gradient ultracentrifugation (100,000 × g, 18 h, 4 °C) using a SW41 rotor (Beckman Coulter, Fullerton, CA, USA) through a continuous 5–40% OptiPrep (Sigma-Aldrich ...
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bioRxiv - Immunology 2022Quote: ... followed by ultracentrifugation at 110,000 × g for 90 min at 4°C on an Optima XPN -100 ultracentrifuge with SW32Ti rotor (Beckman Coulter, Brea, CA, USA). The isolated EVs was washed with PBS and followed with a secondary ultracentrifugation (110,000 × g for 90 min at 4°C) ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were spun down at 4°C for 15 minutes at 4000 RPM (3040 RCF) in a J6 centrifuge (Beckman Coulter Life Sciences). Supernatant was discarded and pellets were stored in 15ml conical tubes in -80°C ...
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bioRxiv - Biochemistry 2020Quote: ... Unbroken cells were pelleted by centrifugation at 18,000 RCF for 30 min at 4°C (Beckman centrifuge J-20-XP, Rotor JA16.250, DE). The supernatant was transferred to 45 Tl rotor (Beckman ...
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bioRxiv - Microbiology 2021Quote: ... Ten milliliters of allantoic or amniotic fluid was centrifuged at maximum speed (16,000 × g) for 30 min at 4 °C using a floor ultra centrifuge (Beckman Optima L-60 Ultracentrifuge). After removal of the supernatant ...
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bioRxiv - Microbiology 2021Quote: ... the SBV samples were purified via sucrose density gradient centrifugation (10–50% range) at 32,500 rpm for 4 h with an SW 41 Ti Swinging-Bucket Rotor (Beckman Coulter Inc., Brea, CA). The SBV band that corresponded to the 40% sucrose gradient was harvested and then centrifuged at 25,000 rpm for 12 h ...
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bioRxiv - Microbiology 2023Quote: ... cells were pelleted by centrifugation at 6,000 ×g at 4 °C for 15 min with ALLEGRA X-30R (Beckman Coulter, Brea, CA, USA). The supernatant was centrifuged at 15,000 ×g at 4 °C for 15 min with Sorvall LYNX4000 (Thermo Scientific ...
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bioRxiv - Bioengineering 2023Quote: ... The diluted sample was then centrifuged using a Beckman ultracentrifuge (Optima L90K) with two cycles of 1h10 at 110,000 x g at 4°C (rotor type 70Ti, Beckman Floor Ultracentrifuge Optima L90K). Between them ...
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bioRxiv - Cell Biology 2024Quote: ... and centrifuged at 16,500 × g (14,500 rpm) at 4°C for 20 min using a Type Ti 45 fixed-angle rotor (Beckman Coulter, k-factor 1.275) to isolate large EVs ...
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bioRxiv - Biophysics 2023Quote: ... Virions were banded by density gradient centrifugation at 152,000 x g for 16 h at 4°C in a SW41 rotor (Beckman Coulter SRL, Milan, Italy). The gradient was then fractionated ...
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bioRxiv - Biochemistry 2023Quote: ... Expression was continued for 7h and the cells harvested by centrifugation at ∼6250g for 15 min at 4°C (Beckman Coulter centrifuge, JLA8.1000).
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bioRxiv - Biochemistry 2023Quote: ... expression from the viral backbone and cells harvested between 72h-84h after cell proliferation arrest by centrifugation at 1200g for 15min at 4°C (Beckman Coulter centrifuge, JLA8.1000). Further ...
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bioRxiv - Biochemistry 2023Quote: ... Expression was continued for 16h and the cells harvested by centrifugation at ∼6250g for 15 min at 4°C (Beckman Coulter centrifuge, JLA8.1000). The RING Zn-finger domains from both RING1B and PCGF4 subunits were cloned into a modified polycistronic expression vector (pET29a ...
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bioRxiv - Microbiology 2022Quote: ... The supernatant fractions were collected and centrifuged at 75,600 x g for 90 min at 4 °C (Beckman JA25.50 rotor, Beckman Avanati J-25, Beckman Coulter). The resulting pellets were washed again and resuspended in 500 μl potassium phosphate buffer ...
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bioRxiv - Microbiology 2022Quote: ... 100 ml cell cultures were grown over night and harvested by centrifugation at 15,000 x g for 15 min at 4 °C (Beckman JLA-16.250 rotor, Beckman Avanti J-25, Beckman Coulter). Pellets were washed with 20 mM potassium phosphate (pH 7.5 ...
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bioRxiv - Microbiology 2022Quote: ... then clarified by centrifugation at 2000 rpm at 4°C for 5 min in a refrigerated tabletop centrifuge (Beckman Coulter, Indianapolis IN, USA) to remove large debris ...
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bioRxiv - Cancer Biology 2022Quote: ... the sample was centrifuged at 268,000 ×g at 4°C for 38 min with an MLA-50 rotor (Beckman Coulter, Brea, CA, USA). The top layer and subsequent 10 fractions (fractions 1–10 ...