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Citations for Beckman :
1101 - 1150 of 1153 citations for Onion Yellow Dwarf Virus OYDV PCR Kits since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: Subsequent second-strand synthesis was performed using the Sequenase 2.0 kit (Thermo #70775Z1000UN) and amplicons cleaned with 1.8 × AMPure XP magnetic beads (Beckman Coulter #A63882). Purified cDNA samples were stored at −20°C until library preparation.
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bioRxiv - Immunology 2024Quote: ... 1 million peripheral immune cells were pipetted into the DuraClone immunophenotyping tube (DuraClone IP Phenotyping kit from Beckman Coulter, USA). Each tube contained anti-CD45-KO ...
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bioRxiv - Immunology 2024Quote: ... Cells were then fixed with the BD Cytofix/Cytoperm™ Fixation/Permeabilization Kit according to the manufacturer’s instructions and intracellularly stained using an anti-HIV-Gag antibody (KC57, Beckman Coulter) before analysis on an Attune NxT flow cytometer.
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bioRxiv - Developmental Biology 2024Quote: ... TH concentrations were measured by a medical laboratory of Perpignan (Médipole) using an ELISA kit (Access Free T3, Access Free T4, Beckman Coulter). TH quantification experiments were replicated three times ...
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bioRxiv - Genetics 2024Quote: Pieces of caudal fin sampled from those 315 fish were sent to Gentyane genotyping platform (INRAE, Clermont-Ferrand, France) for genotyping after DNA extraction using the DNA advance kit from Beckman Coulter following manufacturer instructions.
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bioRxiv - Cancer Biology 2020Quote: ... Amplified libraries were purified with the Qiagen PCR clean-up MinElute kit and size selected for fragments less than 600 bp using the Agencourt AMPure XP beads (Beckman Coulter). Libraries were quality controlled by Qubit and Agilent DNA Bioanalyzer analysis ...
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bioRxiv - Developmental Biology 2021Quote: ... cDNA was prepared using Takara Clontech SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Cat.No.:634890) using 8 cycles of amplification and purified using Agencourt AMPure XP beads (Beckman Coulter, A63880). 1 ng of each sample cDNA was used for library preparation ...
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bioRxiv - Microbiology 2020Quote: ... The resulting cDNA was PCR-amplified to about 10-20 ng/μl using a high-fidelity DNA polymerase followed by purification using the Agencourt AMPure XP kit (Beckman Coulter). The cDNA samples were pooled with ratios according to the RNA concentrations of the input samples and a size range of 200-550 bp was eluted from a preparative agarose gel ...
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bioRxiv - Microbiology 2020Quote: ... The presence or absence of fecal occult blood in fresh pellets was determined using a Hemoccult blood testing kit (Beckman Coulter).
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bioRxiv - Cell Biology 2021Quote: ... or Lin−Sca-1+c-Kit+CD150+CD48−CD34− (CD34−HSC) and Lin−Sca-1+c-Kit+ (LSK) cells were sorted on MoFlo Astrios EQ (Beckman Coulter). Quantification of CD71 ...
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bioRxiv - Microbiology 2020Quote: ... The amplification products were checked using gel electrophoresis and were purified using the Agencourt AMPure XP Kit (Beckman Coulter, CA, USA). The purified products were the indexed in the 16S V4–V5 library ...
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bioRxiv - Genetics 2021Quote: ... The fragment analysis was carried out using capillary electrophoresis in the Beckman Coulter CEQ8000 using the DNA Size Standard Kit-400 (Beckman Coulter) with 1.5 μl PCR product diluted in 20 μl formamide.
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bioRxiv - Cell Biology 2020Quote: ... with anti-Met-PE (clone eBioclone 7, eBiosciences) or isotype control (clone eBRG1, eBiosciences) or permeabilized (IntraPrep Permeabilization kit, Beckman Coulter) before staining (total Met) ...
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bioRxiv - Genomics 2020Quote: ... The library preparation was completed by ligation of adapters from the SQK-LSK109 kit (Oxford Nanopore Technologies) and cleaned up with AMPure XP beads (Beckman Coulter) before preparation for sequencing ...
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bioRxiv - Microbiology 2020Quote: Library preparation was performed using Illumina NexteraXT™ DNA Sample Prep Kit per manufacturer’s protocol followed by AMPure XP (Beckman Coulter) purification ...
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bioRxiv - Cancer Biology 2020Quote: ... 200ng of total RNA was first treated to remove the rRNA and then purified using the Agencourt RNA Clean XP Kit (Beckman Coulter) prior to analysis with the Agilent RNA 6000 Pico Chip to confirm rRNA removal ...
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bioRxiv - Immunology 2022Quote: ... The presence or absence of fecal blood in fresh pellets was determined by macroscopic observation or by applying wet fecal samples to detection tabs from a Hemoccult blood testing kit (Beckman Coulter).
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bioRxiv - Cancer Biology 2022Quote: ... using MiSeq V2 300 cycle reagent kit after the amplified library was purified with AMPure XP beads (Beckman Coulter, CA, USA) (Illumina ...
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bioRxiv - Microbiology 2022Quote: Samples were subjected to 750 μl of lysis buffer supplied with the GenFind V2 DNA extraction kit (Beckman Coulter, Indianapolis, U.S.). The suspension was vortexed for 10 min ...
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bioRxiv - Molecular Biology 2023Quote: ... stained the cells by a Annexin V-FITC/PI Apoptosis Detection Kit (Vazyme, A211-01) and detected cells distribution using a Beckman Coulter Gallios (Beckman Coulter). The result was analyzed by Kaluza Analysis 2.1 (Beckman Coulter).
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bioRxiv - Microbiology 2023Quote: Samples were subjected to 750 μl of lysis buffer supplied with the GenFind V2 DNA extraction kit (Beckman Coulter, Indianapolis, U.S.). The suspension was vortexed for 10 min ...
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bioRxiv - Cell Biology 2023Quote: ... RNA was processed using a NEBNext Ultra II Directional RNA library Prep Kit for Illumina paired with a Poly(A) mRNA magnetic isolation module and AMPure XP SPRIselect beads (Beckman Coulter). Libraries were sequenced using a NextSeq 500 High Output sequencer (Illumina ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Samples were homogenized using metal beads and a bead beater and DNA was extracted using the Agencourt DNAdvance kit (Beckman-Coulter). RNA was removed using RNase followed by an additional bead cleanup ...
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bioRxiv - Microbiology 2024Quote: Bacterial isolates were grown overnight on LB agar and DNA extraction was performed using the GenFind v3 gDNA extraction kit according to standard protocol (Supp. Table 4) (Beckman Coulter). For the mock microbial community ...
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bioRxiv - Neuroscience 2024Quote: ... Sucrose density was prepared according to the Nuclei PURE kit and the lysates were layered over a 1.85M Sucrose Cushion in ultracentrifuge tubes (Beckman Coulter, C13926). High-speed centrifugation was carried out at 30,000 RCF (15,600 RPM ...
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bioRxiv - Genomics 2024Quote: ... was extracted using the Apostle MiniMax cfDNA extraction kit (Apostle, LLC) on a Biomek i7 Hybrid liquid handling system (Beckman Coulter) per manufacturer instructions ...
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bioRxiv - Bioengineering 2024Quote: Frozen TGFb1/ET1-treated and untreated hvCTS (n=7 per condition) and hvCOC (n=5 per condition) were lysed using Agencourt RNAdvance Tissue kit (Beckman Coulter) lysis buffer and homogenized at 1000 rpm for 2 min with 5 mm Stainless Steel Beads (Qiagen ...
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bioRxiv - Molecular Biology 2020Quote: ... The resulting cDNA was PCR-amplified to about 10–20 ng/μl using a high-fidelity DNA polymerase and purified with the Agencourt AMPure XP Kit (Beckman Coulter Genomics). The cDNA samples were pooled based on the ratios according to the RNA concentrations of the input samples ...
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bioRxiv - Microbiology 2021Quote: ... The pulverized tissue was solubilized in pre-heated lysis buffer AL and RNA was extracted using the RNAdvance Tissue Kit (Beckman Coulter, Germany) in combination with a KingFisher Flex Purification System (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2020Quote: ... Cells were then stained both for extracellular and intracellular markers using the PerFix-nc kit following the manufacturer’s protocol (Beckman Coulter, Indianapolis, USA). The following antibodies were used during the staining protocol ...
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bioRxiv - Immunology 2022Quote: The stimulated PBMCs were washed twice with PBS pH7.2 buffer and then permeabilized using the PerFix-nc® reagent kit (Beckman Coulter, USA). Briefly ...
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bioRxiv - Cancer Biology 2024Quote: ... Apoptotic cells were analyzed with the Ebioscience™ Annexin V Apoptosis Detection Kit APC according to the kit instructions (Cat. 88-8007-74, Thermo Fisher Scientific. Samples were read through the CytoFLEX S (Beckman Coulter) instrument using the APC laser to detect the Annexin-V+ cells ...
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bioRxiv - Microbiology 2024Quote: ... The resulting cDNAs were PCR-amplified to about 10-20ng/µl using a high-fidelity DNA polymerase and purified using the Agencourt AMPure XP kit (Beckman Coulter Genomics). Pooled libraries were sequenced on an Illumina NextSeq 500 platform at the Core Unit SysMed at the University of Würzburg for bacterial in vitro samples and on an Illumina Novaseq at the genome analytics core facility of the Helmholtz Centre for Infection Research (HZI ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... we used an AV-FITC/PI apoptosis detection kit to detect programmed cell death using flow cytometry (Cytoflex, Beckman Coulter, CA, USA), as previously described (49 ...
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bioRxiv - Cell Biology 2023Quote: Myf5High and Myf5Low MuSCs were isolated from a pool of 4 male and 4 female mice and repeated in two independent experiments and total RNA was extracted using Agencourt RNAdvance tissue Kit (Beckman Coulter, #A32646) with a quality score of RQN 9.3 to 10 ...
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bioRxiv - Microbiology 2023Quote: ... rRNA was depleted using the NEBNext® rRNA Depletion kit (Bacteria) and purified from the enzymatic reactions using RNAClean XP magnetic beads (Beckman Coulter) following manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... The linearized backbone was extracted from the gel using the Zymogen Gel DNA recovery Kit (#D4001) followed by additional clean up using 1x SPRI bead selection (Beckman Coulter, #B23318) and eluted in TE buffer ...
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bioRxiv - Microbiology 2024Quote: ... PCR amplicon concentrations were quantified on a Qubit Fluorimeter with the Qubit dsDNA Quantification Assay Kit before pooling and purifying with Agencourt AMPure XP SPRI beads (Beckman Coulter, #A63880) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... Apoptotic cells were stained using an Annexin V-FITC Apoptosis Detection Kit (Beyotime, Shanghai, China) and analyzed using a CytoFLEX Flow Cytometer (Beckman, Brea, USA).
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Fetal estrogens are not involved in sex determination but critical for early ovarian differentiationbioRxiv - Developmental Biology 2021Quote: ... Anti Müllerian hormone levels were determined in 50 μl aliquots of serum samples by using an ELISA kit (AMH GenII ELISA, with AMH Gen II calibrators and controls, Beckman Coulter, Villepinte, France) as previously described (Bourdon et al ...
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bioRxiv - Molecular Biology 2020Quote: ... entire ChIPed DNA or 300ng of the input DNA were used to construct libraries using NEBNext® Ultra™ II DNA Library Prep kit with AMPure XP magnetic beads (Beckman Coulter). Library quality and quantity were estimated with Bioanalyzer and Qubit assays ...
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bioRxiv - Cancer Biology 2020Quote: ... DNA was extracted from fresh frozen tissue or cell lines using the Agencourt DNAdvance Beckman Coulter kit (#A48705, Beckman Coulter, Brea, CA, USA), fragmented and submitted to a series of enzymatic steps including End-repair and Adenylation ...
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bioRxiv - Immunology 2020Quote: ... Virus replication kinetics was monitored by SIV Gag p27 production in the supernatant using an SIV core antigen ELISA assay kit (Beckman Coulter, Miami, FL). To assess the properties of SHIV-Ag85B ...
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bioRxiv - Microbiology 2022Quote: ... Libraries were checked on agarose gel for successful amplification and purified with an Agencourt AMPure XP kit (Beckman Coulter Inc., Brea, CA, USA) using the supplier’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... The PCR products were then pooled for each of the 16S rRNA and fungal ITS1 regions after a purification/equalization process with the AMPureXP Kit (Beckman Coulter, Inc., Brea). Primer dimers ...
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bioRxiv - Immunology 2023Quote: ... GEMs were broken and complementary DNA (cDNA) was cleaned up with DynaBeads MyOne Silane Beads (10x Genomics, No. 2000048) and SPRIselect Reagent Kit (Beckman Coulter, No. B23318). Full-length ...
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bioRxiv - Genomics 2024Quote: ... All DNA samples were cleaned three times using Agencourt AMPure beads prior to starting the 1D library kit (Beckman Coulter, Inc., CA, USA). Genomes were assembled with Canu v1.6 with a minimum read length of 5 kbp (45) ...
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bioRxiv - Cancer Biology 2024Quote: ... ATP production was detected with a CellCounting-Lite® 2.0 Luminescent Cell Viability Assay kit (Vazyme Biotech, Nanjing, China) by a plate reader (Beckman Coulter, Krefeld, Germany) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2024Quote: ... were collected from microplates and washed twice in PBS and then resuspended in 50 μl of detergent (DNA-Prep Reagent Kit; Beckman Coulter, Brea, CA) containing 10 ng/ml propidium iodide (DNA-Prep Reagent Kit ...
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bioRxiv - Evolutionary Biology 2021Quote: ... We prepared sequencing libraries using the New England Biolabs Ultra II kit with the only modification being the use of Ampure XP beads for cleanup and size selection (Beckman Coulter, Brea, CA, USA). Following adapter ligation a single size selection was conducted at 0.65X ...