Labshake search
Citations for Beckman :
1101 - 1150 of 1915 citations for 6 Amino 5 2 chloro 4 nitrophenyl azo naphthalene 1 sulfonamide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Systems Biology 2020Quote: ... the 500xg supernatant was first spun at 10,000xg for 10 minutes at 4°C (Beckman Coulter, fixed angle rotor TLA 120.2) in 1 ml 11×34 mm polycarbonate uncapped centrifuge tubes (Beckman Coulter 343778) ...
-
bioRxiv - Microbiology 2019Quote: ... saliva EVs were pelleted by ultracentrifugation at 100,000 × g for 90 min at 4 °C (Optima™ Max-XP, Beckman Coulter). The EVs were washed in 2 ml PBS and pelleted again at 100,000 × g for 90 min and suspended in PBS ...
-
bioRxiv - Systems Biology 2020Quote: ... and underwent ultracentrifugation at 100,000xg for 40 minutes at 4°C (rotor MLA-55; Beckman Coulter 355603 10.4 ml capped tubes). Supernatant was discarded from each fraction ...
-
bioRxiv - Biophysics 2021Quote: The aggregation products formed in Eppendorf tubes under agitation conditions at pH 3 and pH 4 were centrifuged using an Optima MAX-XP ultracentrifuge (Beckman Coulter) in a TLA-55 rotor at 40,000 rpm at 20°C for 45 min ...
-
bioRxiv - Cell Biology 2020Quote: ... 50 µl of alkaline carbonate treated mitochondria were set aside as input (kept on ice) and 50 µl were centrifuged at 100 000g for 30 min at 4°C in TLA100 rotor (Beckman Coulter). Pellet was gently washed with ice-cold sodium carbonate buffer and resuspended in 20 µl of 1x Laemmli loading buffer ...
-
bioRxiv - Immunology 2020Quote: ... during 30min and then transferred into a DuraClone IF T Helper cell tube (CD3, CD4, IL-4, IL-17A, IFNγ) (Beckman Coulter), using the IntraPrep fixation permeabilization kit (Beckman Coulter) ...
-
bioRxiv - Molecular Biology 2021Quote: ... The samples were then separated into membrane and matrix fractions by ultracentrifugation at 100 000 g (TLA 110 rotor) for 30 min at 4°C in OptimaTM TLX table-top ultracentrifuge (Beckman-Coulter). The supernatant fraction was transferred to new tube ...
-
bioRxiv - Bioengineering 2022Quote: ... Cells were subsequently incubated with 2.5×103 CFSE-RJ-EVs per cell for 4 hours and analyzed with flow cytometry (CyAn ADP, Beckman Coulter, US). Treatment groups were compared to unstained control as well as CFSE-RJ-EVs without inhibitor ...
-
bioRxiv - Cell Biology 2022Quote: Whole bovine milk samples (200 mL) were centrifuged at 3,000 x g for 15 min at 4°C (Allegra X-15R, Beckman Coulter, France) to separate the fat from the skimmed milk ...
-
bioRxiv - Biochemistry 2022Quote: ... Soluble and insoluble fractions were isolated via centrifugation (90,000 x g, 30 min, 4 °C) in an Optima™ MAX-OP (Beckman Coulter) centrifuge using the TLA-100.3 rotor ...
-
bioRxiv - Cell Biology 2022Quote: ... The supernatant was recovered and centrifuged at 60,000 rpm for 15 min at 4°C in an Optima TL micro-ultracentrifuges (TLA 100.3 rotor Beckman Coulter, Fullerton, CA) to obtain the Golgi-enriched p100 pellet fraction ...
-
bioRxiv - Physiology 2022Quote: ... 40 ml of filtrates were ultracentrifuged twice in sterile 10.4 mL polycarbonate bottles at 100 000 x g for 70 min at 4°C using an Optima L-100 XP Ultracentrifuge equipped with 90 Ti Fixed-Angle Titanium Rotor (Beckman Coulter). The final EVs pellets were suspended in 200 µl of PBS (Lonza) ...
-
bioRxiv - Microbiology 2022Quote: ... respectively) by centrifugation at 4000 × rpm for 10 min at 4 °C using an Allegra® X-15R centrifuge (Beckman Coulter) and flash-frozen in liquid nitrogen before RNA isolation ...
-
bioRxiv - Neuroscience 2022Quote: ... The discontinuous sucrose gradient was centrifuged at 242K g for 47 min at 4 °C in a vertical rotor (VTi50 rotor, Beckman Coulter). The bulk GC fraction (GCF ...
-
bioRxiv - Microbiology 2022Quote: ... Lysates were then submitted to centrifugation at 100,000 g for 45 min at 4°C in an Ultracentrifuge Beckman XL-90 (Beckman France, Villepinte) equipped with a 70.1T1 rotor ...
-
bioRxiv - Neuroscience 2023Quote: ... Supernatant were loaded on top of a discontinuous 0.75/1.0/2.33 M sucrose density gradient and centrifuge at 242,000 x g for 60 min at 4°C (Beckman SWTi41 swing rotor). The growth cone depleted fraction was collected between 0.75 M and 1.0 M sucrose ...
-
bioRxiv - Physiology 2023Quote: ... was ultracentrifuged twice in sterile 10.4 mL polycarbonate bottles at 100 000 × g for 70 min at 4°C (Optima L-100 XP Ultracentrifuge, 90 Ti Fixed-Angle Titanium Rotor; Beckman Coulter). The final EV pellet was suspended in 50 µl of PBS without Ca2+ and Mg2+ (Lonza ...
-
bioRxiv - Cancer Biology 2023Quote: ... and centrifuged at 100,000 x g either for 4 hr (hollow fiber bioreactor) or overnight (2D flask culture) in a SW32 rotor (Beckman Coulter). For the preparation of the density gradient ...
-
bioRxiv - Plant Biology 2023Quote: ... The cAW was collected by spinning at 700 x g for 20 min at 4 ⁰C (JA-14 rotor, Beckman Coulter; Avanti J-20TM centrifuge, Beckman Coulter). vAW was filtered through a single layer of Miracloth and was combined with the cAW ...
-
bioRxiv - Cell Biology 2023Quote: ... The cilia in the upper phase were sedimented by centrifugation (27,000 g, 4°C, 20 min; Beckman Coulter, Avanti JXN-26) and resuspended in Hepes-Magnesium-EGTA-Potassium (HMEK ...
-
bioRxiv - Biochemistry 2023Quote: ... The sample was run on the gradient for 16 hrs at 4 °C using an Optima XE-90 ultracentrifuge (SW40Ti rotor, Beckman-Coulter) at 30,000 rpm ...
-
bioRxiv - Cell Biology 2023Quote: ... and ultracentrifuged at 200,000 × g for 4 hours using a SW 55 Ti rotor in a Beckman OPTIMA90X ultracentrifuge (Beckman Coulter, USA). Finally ...
-
bioRxiv - Biophysics 2023Quote: ... the SWCNT suspension was centrifuged at 30000 rpm (164 000 x g) for 4 h at 25 °C (Optima XPN-80, Beckman Coulter) to remove any remaining nanotube aggregates.
-
bioRxiv - Genomics 2023Quote: ... cellular/protein debris were then collected by centrifugation at 1,000xg for 15 minutes at 4°C (Beckman Coulter Allegra X-14R), and the supernatant (DNA ...
-
bioRxiv - Biophysics 2022Quote: ... EVs were separated from protein aggregates and free reagents by bottom-up density gradient centrifugation in sucrose for 17.30 h at 192,000 g and 4°C using a SW40 rotor (k-factor 144.5; Beckman Coulter, Fullerton, California, USA). Twelve fractions of 1 mL were then collected from the top of the gradient and respective densities were determined by refractometry using an Atago Illuminator (Japan) ...
-
bioRxiv - Neuroscience 2024Quote: ... The same mass of total extracts was taken for a high spin at 154,000 g for 20 minutes in 4 °C using an ultracentrifuge (Beckman, TLX-120K) to obtain the supernatant soluble fraction (S1 ...
-
bioRxiv - Biochemistry 2023Quote: ... Mitochondrial crude was pelleted by centrifugation at 9500 ×g (4 °C, 15 min) using the Optima XE-90 Ultracentrifuge with a Ti-45 rotor (Beckman Coulter). The final pellet was resuspended in 2 mL of Resuspension buffer (250 mM sucrose ...
-
bioRxiv - Biochemistry 2023Quote: ... then centrifuged at 27,000 gmax for 15 min at 4°C (Optima XPN-100 Ultracentrifuge, Beckman Coulter, with SW60 Ti rotor). Resuspension of the pellet (sub-mitochondrial particles ...
-
bioRxiv - Biochemistry 2023Quote: ... The cells were pelleted by centrifugation at 12,000 xg and 4 °C for 10 min in an Avanti J26-XPI centrifuge (Beckman Coulter Inc.). The cell pellets were homogenized in lysis buffer (25 mM Tris ...
-
bioRxiv - Bioengineering 2023Quote: ... Samples were ultracentrifuged at 100,000×g for 70 min at 4°C using the Beckman OptimaTM L-80 XP ultracentrifuge (Beckman Coulter, CA). The supernatant was discarded ...
-
bioRxiv - Microbiology 2023Quote: ... The normalized cultures were then centrifuged at 5030 x g and 4°C for 20 minutes on an Avanti J-26 XPI (Beckman-Coulter) centrifuge (JS 5.3 rotor) ...
-
bioRxiv - Biophysics 2024Quote: ... The virions were harvested from the supernatant by centrifugation through 1.5ml of 30% sucrose at 10,000 RPM at 4°C for 2hrs (Beckman L8-70M Ultracentrifuge). The resulting pellet of virions was resuspended in 20ul of RIPA lysis buffer containing protease inhibitors.
-
bioRxiv - Molecular Biology 2024Quote: ... for ultracentrifugation at 100,000 rcf for 70 min at 4 °C using a Optima XE-90 ultracentrifuge with a SW 32 Ti rotor (Beckman Coulter). The EV pellet was resuspended in 0.22 μm-filtered sterile PBS and freshly-used or stored at −80 °C until further use ...
-
bioRxiv - Neuroscience 2024Quote: ... and centrifuged at ∼14.000 x g max (∼10900 x g average) for 22 min at 4 °C (Beckman Coulter, MAX-XP). After centrifugation ...
-
bioRxiv - Cell Biology 2024Quote: ... Samples were then spun at 100,000 xg for 16 h at 4°C using a TLS-55 swinging-bucket rotor and benchtop ultracentrifuge (Optima Max-XP, Beckman Coulter). Fractions were collected and loaded in SDS-PAGE for WB.
-
bioRxiv - Cancer Biology 2024Quote: ... CRC cells were then cocultured at a 1:1 ratio with BMDCs for 4 hours and analyzed for uptake of CRC DNA by flow cytometry on the CytoFlex S cytometer (Beckman Coulter). Analysis was performed in FlowJo.
-
bioRxiv - Cell Biology 2024Quote: ... The samples were ultracentrifuged at 39,000 rpm at 4°C for 2.5 h in an SW41 rotor (Beckman Coulter, California, USA). Finally ...
-
bioRxiv - Microbiology 2024Quote: ... Most of the supernatant was discarded leaving 12x∼200 µL of concentrated broken pieces of genomic fiber that were pooled and layered on top of ultracentrifuge tubes of 4 mL (polypropylene centrifuge tubes, Beckman Coulter) containing a discontinuous cesium chloride gradient (1.4 ...
-
bioRxiv - Immunology 2020Quote: ... and anti-CD27-PE-Cy5 (clone 1A4CD27; 2.5 μL; cat. # 6607107) and anti-NKG2A-APC (clone Z199; 5 μL; cat. # A60797) from Beckman Coulter (Supplementary Fig ...
-
bioRxiv - Biochemistry 2020Quote: ... The cultures were grown until OD600 of 0.8 at 37 °C and the cells were collected by centrifugation at 8,000 rpm for 5 minutes in JLA-16.25 rotor (Beckman Coulter), dissolved in 0.5 mL of HEPES:Polymix buffer ...
-
bioRxiv - Microbiology 2021Quote: ... liquid-captured bioaerosols (5 – 10 mL) were centrifuged for 10 minutes at 3000 × g (Allegra X-15R, Beckman Coulter) and resuspended in 200 μL of Middlebrook 7H9 medium supplemented with 100 μM DMN-tre ...
-
bioRxiv - Cell Biology 2021Quote: ... and the mixtures were ultracentrifuged at 80,000 rpm for 5 min at 30 °C using a TLA100 rotor and an Optima TLX ultracentrifuge (Beckman). The microtubule pellets were resuspended in PEM supplemented with 1 mM DTT ...
-
bioRxiv - Cell Biology 2020Quote: ... and 5 % solutions on top of each other in a 13.2 ml open-top polyallomer tube (331372, Beckman Coulter). The 1 ml concentrates were overlaid on top of the gradient ...
-
bioRxiv - Microbiology 2021Quote: ... Linear sucrose gradients were prepared by layering 1.8 ml each of sucrose solutions (wt/wt; prepared in 5 mM EDTA, pH 7.5) into 14-by 95-mm ultracentrifuge tubes (Beckman) in the following order ...
-
bioRxiv - Cancer Biology 2020Quote: ... equivalent amounts of ribosomes for each lysate was loaded onto each 10%-45% sucrose density gradient (BioComp Gradient Master machine) for ultracentrifugation at 33,000 rpm for 5 hours (Beckman Optima L80K Ultracentrifuge ...
-
bioRxiv - Microbiology 2022Quote: ... Cells were grown for additional 1 hour at 30 °C and the biomass was harvested by centrifugation (10,000 rpm, for 5 minutes, JLA-10.500 rotor (Beckman Coulter)).
-
bioRxiv - Biochemistry 2019Quote: ... The cell lysates were subjected to two rounds of centrifugation at 18000 rpm for 5 and 30 mins in a JA-25.50 rotor (Beckman). Bradford assay was used to normalize protein levels for co-immunoprecipitation and immunoblot analysis ...
-
bioRxiv - Genetics 2020Quote: ... 5 μL of each PCR reaction from the single worm lysate was pooled and purified by SPRI (Beckman Coutler) to remove unincorporated oligos ...
-
bioRxiv - Microbiology 2020Quote: ... The sample was vortexed for 10 s and incubated at room temperature for 5 minutes before centrifugation at 12,000 rpm (JA-15 1.5 rotor, Beckman Coulter) 4°C for 5 minutes ...
-
bioRxiv - Cell Biology 2022Quote: ... Resuspended pellets were then subjected to an iodixanol density gradient in 5-mL Ultra-Clear centrifuge tubes (Beckman Coulter). The tubes were first filled with a two-layer gradient ...