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Citations for Beckman :
1101 - 1150 of 2068 citations for 6 9 Methano 4 1 benzoxazepin 2 3H one octahydro 3 methyl 3 α 5a bta 6 bta 9 bta 9a bta 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... The cell lysate was collected in a flask on ice and ultracentrifuged at 4°C for 25 min at 50000 rpm using a Ti-70 rotor (Beckman). The protein purification was done through affinity chromatography on a Ni-column (HiTrap chelating HP column 1 ml ...
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Micro RNAs are minor constituents of extracellular vesicles and are hardly delivered to target cellsbioRxiv - Cell Biology 2020Quote: ... FCS was diluted 1:1 with RPMI and centrifuged at 100,000 g at 4 °C in a swinging-bucket rotor (SW28 or 32, Beckman Coulter) for 18 h ...
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bioRxiv - Microbiology 2021Quote: ... cell lysate was centrifuged 20 min at 14,000 rpm and 4°C to sediment cell debris and supernatant was transferred to microcentrifuge polypropylene tubes (Beckman Coulter) and cytoplasmic membranes were precipitated by ultracentrifugation for 45 min at 55,000 rpm and 4°C (Beckman Coulter rotor TLA 55) ...
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bioRxiv - Molecular Biology 2022Quote: ... The lysate was clarified at 503,000 rcf for 45 min at 4°C using a Type 70 Ti Rotor (Beckman Coulter). The supernatant was incubated with 3 mL IgG Sepharose 6 Fast Flow beads (Cytiva ...
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bioRxiv - Microbiology 2022Quote: ... The capsid-host protein complexes were sedimented through a 30% sucrose cushion at 110,000 g for 20 min at 4°C (TLA-100, Beckman-Coulter), resuspended in CBB by ultrasound tip sonication at 40 W for about 5 x 5 seconds on ice ...
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bioRxiv - Microbiology 2022Quote: ... and C capsids were separated by sedimentation at 50,000 x g and 4°C for 80 min (SW40Ti, Beckman Coulter) on linear 20 to 50% sucrose gradients in TKE buffer (20 mM Tris ...
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bioRxiv - Cell Biology 2022Quote: ... brought up to 3 ml using sterile 20 mM Tris-HCl pH 7.5 and pelleted at 40,000g and 4°C for 60 minutes (TLA100.3 rotor, Optima Max-XP ultracentrifuge, Beckman Coulter, Inc.). Finally ...
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bioRxiv - Cell Biology 2022Quote: ... 1 M sucrose) and centrifuged at 100,000 RPM for 60 minutes at 4°C in a TLA100.3 rotor (Beckman Coulter, 349490) with 3.5 mL polycarbonate tubes (Beckman Coulter ...
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bioRxiv - Neuroscience 2020Quote: ... The V and VI fractions were diluted to a total volume of 12 mL with dfPBS and centrifuged at 140,000 × g for 70 min at 4°C (# Optima-XE SW41 Beckman Coulter), and each pellet were resuspend with 30 μl of dfPBS ...
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bioRxiv - Molecular Biology 2020Quote: ... The following day the viral pellet was resuspended in 5 ml PBS and centrifuged at 20,000 rpm for 90 mins at 4°C (Optima XL-100K, Beckman Coulter). The pellet was then resuspended in the required volume of PBS before being aliquoted and stored at −70°C.
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bioRxiv - Microbiology 2021Quote: ... The membrane fraction was prepared using ultracentrifugation (45,000 rpm, 4 °C, 1h; Optima-90 ultracentrifuge, Beckman-Coulter, Brea, CA, USA) and washed once in 200 mM ABC buffer ...
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bioRxiv - Biochemistry 2021Quote: ... The sucrose loaded heavy MLVs were centrifuged at 180,000 x g for 30 min at 4°C using Optima TL benchtop Ultracentrifuge (Beckman Coulter). The resulting pellets were buffer exchanged by resuspension into the assay buffer comprising 50 mM HEPES pH 7.5 ...
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bioRxiv - Molecular Biology 2021Quote: ... Insoluble material was removed by centrifugation (100,000 x g; 30 min; 4°C; 45 Ti rotor; Optima XPN-80; Beckman Coulter). The cell lysate supernatant was passed through a Ni+2-NTA resin column (Qiagen) ...
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bioRxiv - Molecular Biology 2021Quote: ... The membrane fraction was pelleted down by high-speed centrifugation (100,000 x g; 4°C; 30 min; 45Ti rotor; Optima XPN-80, Beckman Coulter). The membrane pellet was resuspended in solubilization buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... Ultra-centrifugation was performed (160,000 x g; 16 hours; 4°C; SW 32 Ti rotor; Optima XPN-80, Beckman Coulter) and 1mL fractions were collected and analyzed by BN-PAGE ...
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bioRxiv - Molecular Biology 2019Quote: ... followed by ultracentrifugation at 100,000 g for 2h in polyallomer tubes at 4 °C in a SW40 rotor (Beckman Coulter). Pelleted material was washed two times in filtered PBS at 100,000 g for 2h and re-suspended in PBS ...
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bioRxiv - Cell Biology 2019Quote: ... and spun down at 4° C for 10 minutes at 12096×g using an Avanti J-25 centrifuge (Beckman Coulter). The sonicated lysate was incubated for 2 hours at 4 ° C with beads (Glutathione High Capacity Magnetic Agarose Beads ...
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bioRxiv - Molecular Biology 2019Quote: ... and then centrifuged for 150 min at 39,000 rpm and 4°C in an Optima L-100XP Ultracentrifuge (Beckman-Coulter) using a SW41Ti rotor without brake ...
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bioRxiv - Biochemistry 2019Quote: ... The supernatant from 2,000 × g was transferred to 30mL conical tube and then centrifuged at 10,000 × g for 10 min at 4°C (# Avanti J-E JA25-50 Beckman Coulter). The supernatant from this spin was filtered through a 0.22-μm mesh filter (# SLGP033RS EMD Millipore ...
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bioRxiv - Biochemistry 2019Quote: ... The V and VI fractions were then diluted separately to a total volume of 12mL with dfPBS and centrifuged at 100,000 × g for 70 min at 4°C (# Optima-XE SW41 Beckman Coulter). The final pellet from each fraction was resuspended in 30 μL of dfPBS ...
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bioRxiv - Cell Biology 2020Quote: ... The supernatant of MNase treated chromatin were overlaid onto the sucrose gradient and spun at 32000 rpm (124,436 rcf) for 13 h at 4 °C using SW55Ti rotor (Beckman Coulter) in Beckman Optima L80.
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bioRxiv - Cell Biology 2020Quote: ... 1 mM EGTA) and spun at 32000 rpm (124,436 rcf) and 4 °C for 13 h using SW55Ti rotor (Beckman Coulter) in Beckman Optima L80 ...
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bioRxiv - Microbiology 2021Quote: ... The treated cells were pelleted by centrifugation at 12,000×g at 4 °C for 20 min (Beckman Allegra X-15R) and the supernatant was discarded ...
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bioRxiv - Plant Biology 2020Quote: ... The insoluble cell debris was removed via centrifugation for 30 min at 4°C and 20,000 rpm (JA-25.50, Beckman Coulter®). The supernatant was incubated for 1 h with low agitation and with 1.2 ml Glutathione-sepharose (GE-Healthcare ...
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bioRxiv - Plant Biology 2020Quote: ... the cells were harvested by centrifuging for 30 min at 4°C and 6,000 rpm (JA-10, Beckman Coulter®). The cells were resuspended in 1x PBS (pH 7.3 ...
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bioRxiv - Genomics 2021Quote: ... The tubes were then balanced and centrifuged in a Beckman Coulter L7-65 Ultracentrifuge at 25,000rpm at 4°C for 2hr using a Beckman Coulter SW28 swing bucket rotor (Beckman Coulter). The supernatant was carefully removed via aspiration ...
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bioRxiv - Genomics 2019Quote: ... 37°C for 30 min then cooled to 4°C before proceeding to a 2.2x Agencourt RNAClean XP cleanup (Beckman Coulter). The RNA was eluted in 11.5 μL nuclease-free water ...
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bioRxiv - Biochemistry 2019Quote: ... Ribosomes were pelleted via centrifugation at 63,000 x g for 16-18 hours at 4°C using a Type 80 Ti rotor (Beckman Coulter). Ribosome pellets were dissolved in resuspension buffer (20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biochemistry 2019Quote: ... Fractions containing 40S and 60S ribosomal subunits as identified via absorption at 254 nM were pelleted via centrifugation at 63,000 x g for 20 hours at 4°C using a Type 80 Ti rotor (Beckman Coulter). Ribosomal subunits were resuspended in storage buffer (30 mM HEPES-KOH pH 7.4 ...
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bioRxiv - Biochemistry 2019Quote: ... The homogenate was centrifuged for 10 min at 10,000×g at 4°C in a Ti-70 rotor and Optima XPN-90 ultracentrifuge (Beckman Coulter) and the supernatant was removed ...
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bioRxiv - Molecular Biology 2020Quote: ... by centrifuging at 63,000 x g for 18 hrs at 4°C using a Type 80 Ti rotor (Beckman Coulter). Pellets were resuspended and these samples were analysed by mass spectrometry.
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bioRxiv - Immunology 2021Quote: ... Ultracentrifugation in a Beckman L7-65 ultracentrifuge at 25,000 rpm for two hours at 4°C using a Beckman SW28 rotor (Beckman Coulter) was performed to pellet the viruses through the sucrose cushion while soluble egg proteins were removed ...
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bioRxiv - Cell Biology 2020Quote: ... The ultracentrifugation (UC) steps were performed in refrigerated conditions (4°C) with rotors 50.4Ti or 70Ti (Beckman-Coulter, California, US).
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bioRxiv - Physiology 2021Quote: ... the supernatant was ultracentrifuged at 100,000 g for 70 min at 4°C using an MLA-55 rotor (Beckman Coulter) and an Optima MAX-TL Ultracentrifuge (Beckman Coulter) ...
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bioRxiv - Biochemistry 2021Quote: ... Samples were ultracentrifuged at 120,000 × g for 90 minutes at 4 °C in an Optima XPN-80 ultracentrifuge (Beckman Coulter) using a SW55 Ti swinging-bucket rotor (Beckman Coulter) ...
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bioRxiv - Biochemistry 2021Quote: ... and cellular debris was removed by centrifugation at 90 000 x g for 20 min at 4°C (45 Ti rotor, Beckman). Membranes were collected by ultracentrifugation at 220 000 x g for 90 min at 4°C (45Ti rotor ...
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bioRxiv - Cell Biology 2020Quote: ... This crude mitochondrial pellet was further purified on a discontinuous percoll gradient (buffer composition 15/23/40% sucrose) by centrifugation (40,000 x g, 1hr, 4°C) in an SW41 rotor (Beckman Coulter) and enriched mitochondria were collected from the 23-40% interface (adapted from [76]) ...
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bioRxiv - Cell Biology 2020Quote: ... Tubes were centrifuged twice at 100,000 x g for 70 min at 4°C with a Beckman ultracentrifuge (32Ti rotor from Beckman Coulter). Supernatants were removed and the pellets resuspended in phosphate-buffered saline (PBS ...
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bioRxiv - Microbiology 2020Quote: ... Microbial cells were collected from to 2-3 L of water samples by centrifugation at 12,000 × g and 4°C for 20 min in a high-speed centrifuge (Beckman, USA). Total genomic DNA was extracted using AxyPrep™ Genomic DNA Miniprep Kit (Axygen ...
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bioRxiv - Microbiology 2020Quote: ... Two distinct fractions were collected from the first CsCl density gradient centrifugation (17,000 × g, 60 min, 4°C in a fixed angle MLA-55 rotor, Beckman Coulter), each were sedimented (4000 × g ...
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bioRxiv - Molecular Biology 2021Quote: ... lysates were spun for 24 h at 4°C at 50000 rpm in a Type 70 Ti rotor (Beckman Coulter) through a 40 % (w/v ...
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bioRxiv - Microbiology 2021Quote: ... purified by ultracentrifugation through a sucrose cushion (25% weight/volume in Tris-NaCl-EDTA buffer) for 75 min at 4°C and 28,000 rpm using a SW 32 TI rotor (Beckman Coulter), resuspended in serum-free RPMI 1640 or DMEM medium and stored in small aliquots at −80°C ...
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bioRxiv - Immunology 2020Quote: ... previously coated over-night at 4 °C with 1.5 μg per well of anti-Igκ or anti-IgM (Beckman Coulter) in PBS and saturated with culture medium ...
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bioRxiv - Microbiology 2021Quote: ... The resting supernatants were filtered through 0.45µm syringe filters and ultracentrifuged at 100,000 x g for 1h at 4°C (SW41 Ti swinging-bucket rotor, Beckman Coulter). The supernatant was discarded and the pellet suspended in 0.22 µm-pore filtered or 0.02 µm-pore filtered (for Flow Cytometry analysis ...
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bioRxiv - Biochemistry 2022Quote: ... A soluble fraction was obtained after centrifugation of the lysate at 19,000 rpm for 40 min at 4 °C performed using Avanti J-E with a rotor JA-20 (BECKMAN COULTER) to remove insoluble debris.
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Dynamic states of eIF6 and SDS variants modulate interactions with uL14 of the 60S ribosomal subunitbioRxiv - Biochemistry 2022Quote: ... Ribosomes were pelleted by centrifugation at 63,000g for 16–19 hrs at 4°C using a Type 80 Ti rotor (Beckman Coulter). For all ultracentrifugation steps indicated here ...
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Dynamic states of eIF6 and SDS variants modulate interactions with uL14 of the 60S ribosomal subunitbioRxiv - Biochemistry 2022Quote: ... were pelleted by centrifugation at 63,000g for 20 hrs at 4°C in a Type 80 Ti rotor (Beckman Coulter) as described above ...
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bioRxiv - Cell Biology 2022Quote: ... OptiPrep step-gradient and centrifuged for 60 min at 19,210 × g at 4 °C in a TLS-55 rotor (Beckman Coulter). The ROS band was collected with a 18G needle ...
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bioRxiv - Cell Biology 2022Quote: ... The lysate was dounced for 10 strokes on ice and cleared via centrifugation at 80K rpm for 10 minutes at 4°C using a TLA 110 rotor (Beckman) in a tabletop Beckm an ultracentrifuge ...
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bioRxiv - Microbiology 2022Quote: ... purified by ultracentrifugation through a sucrose cushion (25% weight/volume in Tris-NaCl-EDTA buffer) for 75 min at 4°C and 28,000 rpm using a SW 32 TI rotor (Beckman Coulter), resuspended in serum-free DMEM medium and stored in small aliquots at −80°C ...