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Citations for Beckman :
1101 - 1150 of 1407 citations for 4 Methoxy 6 methyl 6 phenyl 5H pyran 2 one since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... cells were resuspended in 100µL of PBS and stored at 4°C in the dark until samples were analyzed on a CytoFLEX LX Flow Cytometer (Beckman Coulter). Data was analyzed using FlowJo® software.
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bioRxiv - Bioengineering 2024Quote: ... we first measured a cell line population in PBS suspension using a coulter counter (Beckman Coulter Multisizer 4, 100 μm aperture) to obtain a cell volume distribution with the standard unit ...
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bioRxiv - Cell Biology 2024Quote: ... We loaded 2 mL of the homogenate mixture on top of a 3.92 ml 62% sucrose in MAB cushion in an Ultra-Clear 5/8” X 4” Beckman Coulter tube (344061, Beckman Coulter). We added the remaining sucrose in MAB solutions ...
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bioRxiv - Biochemistry 2024Quote: ... Non-solubilized materials were removed by centrifuging the sample at 41,000 r.p.m for 1 h at 4 °C using rotor type 45 Ti in the OptimaTM L-100 XP Ultracentrifuge (BECKMAN COULTER). The resulting supernatant containing the solubilized LysP-GFP was incubated with Ni2+-NTA resin (Qiagen ...
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bioRxiv - Microbiology 2024Quote: ... Samples were balanced and centrifuged at 42,000 rpm for 35m at 4°C in a SW55Ti rotor and buckets with tube adaptors (Beckman REF: 356860). The supernatant was then concentrated to 80μl by spinning through Amicon 3kDa MWCO concentrator units at 14,000xg for 15-30m at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... and labeled intracellularly for the structural HIV-1 p24 protein with the anti-p24 clone KC57 antibody (30 min RT followed by 30 min 4°C, Beckman Coulter). HIV-1 RNA probing was performed using the PrimeFlow RNA Assay (ThermoFisher) ...
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bioRxiv - Microbiology 2024Quote: ... and resolved by ultracentrifugation at 36,000 rpm for 3 h at 4°C with the fastest braking in Optima XPN-80 Ultracentrifuge equipped with SW-41Ti rotor (Beckman Coulter). Gradients were fractionated (0.5 mL/fraction ...
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bioRxiv - Plant Biology 2024Quote: ... The tubes were then centrifuged for 20 min at 700 x g with slow acceleration at 4°C using a JA-14 rotor (Avanti J-20 XP centrifuge; Beckman Coulter). The resulting apoplastic wash collected was passed through a 0.22 µm membrane filter to remove bacteria and larger debris ...
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bioRxiv - Microbiology 2024Quote: Bacterial isolates were grown overnight on LB agar and DNA extraction was performed using the GenFind v3 gDNA extraction kit according to standard protocol (Supp. Table 4) (Beckman Coulter). For the mock microbial community ...
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bioRxiv - Microbiology 2024Quote: ... This sample was then ultracentrifuged at 150,000 g for 2.5 h at 4 °C using a Type 70 Ti or Type 45 Ti rotor in an Optima XE Ultracentrifuge (Beckman Coulter). The translucent VLP pellet formed by this process was resuspended in 200 μl PBS buffer ...
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bioRxiv - Immunology 2024Quote: ... Centrifugation was performed (30 min, 800 g, 4 °C) in a swinging bucket on a Beckman Coulter JS-13.1 rotor (Beckman Coulter, USA). Hemocytes concentrated at each density interface were collected separately with a 70 mm long 20Gx2.75” needle mounted on a 1 mL syringe ...
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bioRxiv - Molecular Biology 2024Quote: ... The obtained culture was pelleted at low speed (7000 rpm for 10 minutes at 4 0C in a Beckman Coulter centrifuge). Harvested cells were resuspended (1 g of cell pellet per 10 mL ...
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Sense codon-misassociated eRF1 elicits widespread ribosome stalling and induction of quality controlbioRxiv - Molecular Biology 2024Quote: ... The mixture was layered over 900 μl of 1 M sucrose cushion 46 and ultracentrifuged at 100,000 rpm ( 543,000 × g) at 4°C for 1 h using a TLA-110 rotor and an Optima MAX-TL Ultracentrifuge (both Beckman Coulter).
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The total number of microglia (CD11b+ CD45+ cells) in the L3/4 spinal cord or SDH was analyzed using CytoFLEX SRT (Beckman Coulter) and FlowJo software (TreeStar) ...
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bioRxiv - Molecular Biology 2024Quote: ... The tube was centrifuged at 38,000 x g for 21-24 h at 4°C using the SW40T1 rotor in the Optima L-60+ Ultracentrifuge (Beckman Coulter). After ultracentrifugation ...
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bioRxiv - Neuroscience 2024Quote: ... The combined supernatants were centrifuged at 100,000 g for 40 min (4°C) on a TL110 rotor (Beckman Coulter, Paris, France) and the pellet was suspended in the same buffer ...
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bioRxiv - Microbiology 2024Quote: ... the supernatant was filtered through 0.45-μm syringe filters and ultracentrifuged at 100,000 ×g for 1 h at 4 °C (MLA-50 fixed angle rotor, Beckman Coulter, Germany). The supernatant was discarded ...
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bioRxiv - Biochemistry 2024Quote: ... After centrifugation at 60,000 x g for 20 min at 4°C in a swing-out rotor (MLS-50, Optima Max-XP, Beckman Coulter), the mitochondrial fraction localized in the interphase between the two sucrose layers was carefully removed with a syringe ...
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bioRxiv - Cell Biology 2024Quote: ... The samples were centrifuged at 4°C for 24 h at 274,400 x g in a SW-40Ti rotor (Beckman Coulter Inc.). To remove KBr ...
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bioRxiv - Cell Biology 2024Quote: ... cells were resuspended in PBS and stored in the dark at 4°C until analyzing them with a Navios flow cytometer (Beckman Coulter). At least 10’000 living cells were measured for each sample ...
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bioRxiv - Pathology 2021Quote: ... BALF (10 μl) was applied to glass microscopy slides using a cytocentrifuge (StatSpin CytoFuge 2, Beckman Coulter). Slides were fixed with 10% neutral buffered formalin ...
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bioRxiv - Developmental Biology 2021Quote: ... inhibin B and AMH/MIS by 2-site ELISA (Beckman Coulter and Beckman Coulter Gen II, respectively); and βHCG and AFP CMIA-ARCHTECT I 4000.The assays were performed as recommended by the manufacturers ...
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bioRxiv - Microbiology 2022Quote: ... 2 ng of each product were pooled for subsequent cleanup using the AmpureXP PCR cleanup protocol (Beckman). The pooled library was quantified by a picogreen assay and loaded onto an Illumina MiSeq Reagent Kit v3 (600-cycle ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatant was ultracentrifuged for 2 h at 150,000 x g in an Optima XP centrifuge (Beckman) with a MLA-50 rotor ...
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bioRxiv - Microbiology 2022Quote: ... cells were resuspended in 2% FBS-PBS and analyzed by a CytoFLEX flow cytometer (Beckman Coulter, USA). The final results from three independent experiments were averaged and shown in the histogram.
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bioRxiv - Cancer Biology 2024Quote: ... Cells were then resuspended in PBS + 2% FBS and acquired on a Gallios flow cytometer (Beckman Coulter).
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bioRxiv - Molecular Biology 2023Quote: ... Reactions were incubated for 2 hr at 60°C and purified by Ampure XP beads (Beckman #A63881). Libraries were PCR amplified in a 30 µL reaction containing 14 µL circularized library ...
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bioRxiv - Molecular Biology 2023Quote: ... Reactions were incubated for 2 hr at 60°C and purified by Ampure XP beads (Beckman #A63881). Libraries were PCR amplified in a 30 µL reaction containing 14 µL circularized library ...
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bioRxiv - Biochemistry 2023Quote: ... The cell culture microplate was centrifuged at 300g for 2 min (Beckman Coulter Allegra X-22R Centrifuge) to bring the cells to the well bottom ...
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bioRxiv - Biophysics 2024Quote: ... Cells were pelleted from 2 L cultures by centrifugation at 4,785g (5,000 rpm in Beckman JLA-8.100) for 20 min ...
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bioRxiv - Molecular Biology 2024Quote: ... and gradients were centrifuged at 35,000 RPM for 2 hours 45 min using a SW41Ti rotor (Beckman). Absorbance was followed at 254 nm ...
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bioRxiv - Microbiology 2024Quote: ... The filtrate was ultracentrifuged at 200,000 xg for 2 hr (Optima L-100 XP ultracentrifuge; Beckman Coulter). Supernatants were discarded ...
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bioRxiv - Biochemistry 2024Quote: ... The supernatant containing the membrane fraction was transferred to Beckman ultracentrifuge tubes and subjected to ultracentrifugation for 2 h at 41,000 r.p.m (Beckman Optima L-100 XP ultracentrifuge ...
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bioRxiv - Biophysics 2021Quote: ... which were centrifuged at 3000 rpm for 15 minutes at 4°C using a fixed angle centrifuge (Beckman Coulter, Brea, CA, USA). After removal of excess supernatant ...
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bioRxiv - Biophysics 2020Quote: ... The lysates were clarified by centrifugation (16,000 rpm for 1 h at 4°C, rotor JA 25.50, Beckman Coulter, Brea, California, USA). The supernatant was incubated with amylose resin for 1 h at 4°C and loaded onto a 20 ml chromatography column (Bio-Rad ...
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bioRxiv - Cancer Biology 2021Quote: Proliferation of IL-32 KO and WT mock cells was assessed by cell counting every day for 4 days using cell coulter (Beckman Coulter Diagnostics). Cell viability was assessed by Trypan blue staining or by annexin/PI flow cytometry and analyzed by FACS Diva software (BD Biosciences).
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bioRxiv - Systems Biology 2020Quote: ... Next the cultures were diluted 1:40 with 0.5M NaCl and immediately measured in Multisizer™4 COULTER COUNTER® (Beckman Coulter) (Coulter ...
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bioRxiv - Biophysics 2022Quote: ... The tubes were filled with PBS to reach the final volume and samples were ultracentrifuged at 120,000 g for 60 minutes at 4°C (70.1 Ti rotor, k-factor 36, Beckman Coulter, Brea, CA, USA). Finally ...
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bioRxiv - Plant Biology 2020Quote: ... All samples were centrifuged in a single 2 h run with sedimentation at 50,000 g and 4°C using small 13 x 51 mm ultracentrifugation tubes in SW55 Ti rotor (Beckman Coulter, Krefeld, Germany). These centrifugation settings were optimized for polysome and monosome separations ...
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bioRxiv - Cell Biology 2020Quote: ... Vacuoles were separated from free dye by centrifugation (100,000 x g, 25 min, 4°C, SW-60 Ti rotor, Beckman L8-80M Ultracentrifuge) and recovered from the 0%-4% interface ...
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bioRxiv - Biophysics 2021Quote: ... then the supernatant was ultracentrifuged at 100000×g for 20 min at 4 °C using an Optima L-100K ultracentrifuge (Beckman Coulter, USA) equipped with a SW 40 Ti swinging bucket rotor ...
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bioRxiv - Plant Biology 2021Quote: ... The resulting slurry was centrifuged for 15 min at 9000 rpm at 4 °C using a JA14 rotor (Beckman Coulter, Brea, CA). The supernatant was filtered with a Miracloth and kept on ice ...
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bioRxiv - Plant Biology 2021Quote: ... sucrose was transferred to the bottom of the tube using a Pasteur pipette and centrifuged at 27,000 rpm for 3 hours at 4° C using an SW28 Ti rotor (Beckman Coulter, Brea, CA). Each pellet containing the virus particles was resuspended in 1 mL resuspension buffer overnight with gentle shaking at 4 °C ...
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bioRxiv - Biochemistry 2021Quote: ... Lentivirus from the cell supernatant was collected 24h later by ultracentrifugation with a Sw28 rotor (Beckman Coulter; 22,000 rpm, 2h, 4°C). Finally ...
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bioRxiv - Bioengineering 2021Quote: ... The resulting supernatant was then ultracentrifuged at 50,000 RPM and 4 °C for 90 minutes using a 70.1 Ti rotor (Beckman Coulter, Brea, CA, USA) with 1 mL 15% sucrose cushion per tube ...
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bioRxiv - Cell Biology 2021Quote: ... The samples were centrifuged at 60,000 rpm at 4°C for 2hr in an SW65 Ti ultracentrifuge rotor (Beckman Coulter, California, USA). The translucent polysome pellets were washed by gently dispensing and removing 500 µl of ice-cold RNase-free water ...
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bioRxiv - Microbiology 2020Quote: ... RNA was pelleted by centrifugation for 24 h at 4 °C and 104.000 g in a SW28.1 swing out rotor (Beckman Coulter, Brea, CA, USA). RNA was taken up in 100 µl H2O and 500 µl TS buffer was added ...
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bioRxiv - Molecular Biology 2020Quote: ... the serum was centrifuged (200,000xg, at 4°C for 18 hours) using a Beckman Coulter Optima XE-100 Ultracentrifuge (Beckman Coulter, CA, USA), with a SW41Ti rotor (Beckman Coulter ...
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bioRxiv - Neuroscience 2022Quote: ... The crude viral lysate was purified with two rounds of CsCl gradient ultracentrifugation at 100,000×g and 4°C for 23 h using 13.5-mL Quick-Seal tubes and a NVT65 rotor (Beckman Coulter, Brea, CA). Each tube was placed gently and 500– 750 µL of white turbid bands were collected ...
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bioRxiv - Molecular Biology 2022Quote: ... were pre-clarified to remove aggregate proteins by spinning at 65,000 rpm for 30 min at 4°C in a TLA100 rotor (Beckman Coulter, Brea, CA) before incubating with F-actin ...