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Citations for Beckman :
1101 - 1150 of 1331 citations for 4 4 7 Triethoxy 7 Methyl 3 8 Dioxa 4 7 Disiladecane since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... Virions were banded by density gradient centrifugation at 152,000 x g for 16 h at 4°C in a SW41 rotor (Beckman Coulter SRL, Milan, Italy). The gradient was then fractionated ...
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bioRxiv - Biochemistry 2023Quote: ... expression from the viral backbone and cells harvested between 72h-84h after cell proliferation arrest by centrifugation at 1200g for 15min at 4°C (Beckman Coulter centrifuge, JLA8.1000). Further ...
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bioRxiv - Biochemistry 2023Quote: ... Expression was continued for 16h and the cells harvested by centrifugation at ∼6250g for 15 min at 4°C (Beckman Coulter centrifuge, JLA8.1000). The RING Zn-finger domains from both RING1B and PCGF4 subunits were cloned into a modified polycistronic expression vector (pET29a ...
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bioRxiv - Microbiology 2024Quote: ... The solution was then given a final concentration of 25 mM MgCl2 and ultracentrifuged at 200,000 x g for two hours (4°C) (Beckman Coulter Type 45 Ti). The pellet was then resuspended in 100 μl of distilled water.
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bioRxiv - Microbiology 2024Quote: ... The 1 ml fraction containing the phage was then placed on a 40% (w/v) - 60% (w/v) sucrose gradient for 1 hour at 63,400 x g at 4°C (Beckman Coulter SW 40 Ti). The 1 ml fraction was then placed in a 50 kDa dialysis membrane (Spectrum Labs ...
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bioRxiv - Microbiology 2024Quote: ... The supernatant was collected and treated with polyethyleneimine to a final concentration of 0.03% incubated on ice for 5 minutes and subsequently centrifuged at 10,000 rpm at 4°C for 10 minutes (Beckman coulter, JA-25.50 rotor). The supernatant was collected and powdered ammonium sulfate (approx 15 g ...
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bioRxiv - Neuroscience 2024Quote: ... cells were harvested and lysed in the lysis buffer described above and ultracentrifuged at 100,000 x g for 30 min at 4 °C using the TLA-110 rotor and the Optima™ MAX-XP tabletop ultracentrifuge (Beckman, Brea, CA). The supernatants were collected as “triton-soluble fraction” and the pellets were washed with the lysis buffer ...
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bioRxiv - Microbiology 2024Quote: ... was added to the supernatant on ice with constant stirring until protein precipitate was observed followed by centrifugation at 10,000 rpm at 4°C for 10 minutes (Beckman coulter, JA-25.50 rotor). The pellet was resuspended in 4 ml of binding buffer (20 mM Tris-HCl ...
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bioRxiv - Plant Biology 2024Quote: ... The supernatant was fractionated at 200 k x g for 20 minutes at 4°C using a Beckman Optima Ultracentrifuge with TLA110 rotor (Beckman Coulter Life Sciences) into a cytosolic (s200 ...
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Scalable Fabrication of a Tough and Recyclable Spore-Bearing Biocomposite Thermoplastic PolyurethanebioRxiv - Bioengineering 2024Quote: ... Spore suspension was centrifuged at 12,000 g at 4 °C for 20 min (Avanti J-E, Beckman Coulter Life Science, Brea, CA, USA). After removing supernatant ...
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bioRxiv - Biochemistry 2024Quote: ... Nucleosome sucrose gradients were then centrifuged at 41,000 rpm and 4°C for 22 h in a SW41 Ti rotor in an Optima L-100 XP ultracentrifuge (Beckman Coulter, Brea, CA). After centrifugation ...
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bioRxiv - Biochemistry 2024Quote: ... The A2aR-expressing membrane fraction was obtained by ultra- centrifugation at 100,000 g for 1 hour at 4°C in a Type 70 Ti Fixed-angle rotor (Optima XPN ultracentrifuge, Beckman Coulter Life Sciences). The pellet (membrane fraction ...
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bioRxiv - Biochemistry 2024Quote: ... samples from different temperatures for each drug treatment were pooled and subjected to ultracentrifugation at 100,000g for 20 minutes at 4°C (Beckman Coulter Optima Max-XP). The soluble proteins from the supernatant were collected for further analysis.
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bioRxiv - Cell Biology 2024Quote: ... The supernatant was subsequently ultracentrifuged at 30,000 × g for 60 min at 4°C in an SW32Ti rotor (Beckman Coulter, Brea, CA, USA) to obtain L-EVs ...
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bioRxiv - Cell Biology 2024Quote: ... Microsomes were enriched at 200 k x g for 20 minutes at 4°C using a Beckman Optima Ultracentrifuge with TLA110 rotor (Beckman Coulter Life Sciences) and washed twice with MIB ...
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bioRxiv - Biochemistry 2020Quote: ... disrupted with a tip sonicator at 1/3 power (8 MHz) for 3 × 30 seconds on ice and fractionated by ultracentrifugation (100’000 x g, 1H, Beckman TLA 120.2 rotor). The luminal fraction was concentrated to around 50 µL on a 3 kDa MWCO Amicon Ultra 0.5 mL centrifugal filter while membrane pellets were dissolved in 50 µL TBS with 2% SDS ...
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bioRxiv - Neuroscience 2020Quote: ... The number and size distribution of the clot fragments that break off the thrombus during thrombectomy are quantified using a Coulter counter (Beckman Multisizer 4, Brea, California). Each removal technique is repeated 8 times for each clot type ...
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bioRxiv - Cell Biology 2020Quote: ... Bacteria were harvested by centrifugation at 4000 rpm for 25 min at 4°C in a Beckman J6M clinical centrifuge (Beckman Coulter Diagnostics; Brea, California), and re-suspended on ice in lysis buffer (50 mM Tris pH 7.5 ...
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bioRxiv - Microbiology 2020Quote: ... and layered on top of a two-step sucrose gradient (20%/50% w/v in HNE) and centrifuged for 2 h at 154 000 x g at 4 °C in a SW41 rotor (Beckman Coulter, Woerden, the Netherlands) to clear from cell debris ...
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bioRxiv - Systems Biology 2020Quote: ... and the resultant supernatant was ultracentrifuged at 100,000xg for 40 minutes at 4°C (rotor TLA 120.2; Beckman Coulter 343778 1 ml uncapped tubes).
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bioRxiv - Physiology 2021Quote: ... sEV were further isolated from lEV-depleted supernatants following a 100,000 xg ultracentrifugation step for 1 hour at 4°C (rotor MLA-50, Beckman Coulter Optima MAX-XP Ultracentrifuge) and two washes in NaCl before resuspension in NaCl.
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bioRxiv - Neuroscience 2022Quote: ... The samples were then spun at 31000 x g (18468 rpm) for 5 min at 4°C in a fixed-angle rotor (50.2 Ti rotor in Beckman Coulter Optima L-100 XP) with slow brake ...
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bioRxiv - Plant Biology 2024Quote: ... The size of the cell population and the number of intact cells were measured using a Coulter Counter (Multisizer 4; Beckman Counter, Brea, CA, USA).
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bioRxiv - Biophysics 2023Quote: ... Cell debris was removed by centrifugation at 20,000 rpm at 4°C for 1 h (Avanti® J-E centrifuge from Beckman Coulter, rotor: JLA 25.50). The supernatant was collected and loaded onto a pre-equilibrated 5 ml His-Trap FF crude column ...
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bioRxiv - Molecular Biology 2023Quote: ... and were centrifuged at 120,000 g for 120 minutes at 4°C with a MLA55 fixed-angle rotor using an Optima MAX-XP ultracentrifuge (Beckman Coulter, California, United States). The pelleted hVSMCs EVs were resuspended in 100 μL PBS and washed in 13.2 mL PBS by ultracentrifugation at 120,000 g for 60 minutes at 4°C ...
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bioRxiv - Plant Biology 2024Quote: ... placed inside 250 mL centrifuge bottles and centrifuged for 10 min at 100 g at 4 °C (JA-14 rotor, Avanti J-20 XP Centrifuge; Beckman Coulter, Indianapolis, IN, USA). LSW was then filtered through a 0.2 µm syringe filter (Acrodisc syringe filter ...
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bioRxiv - Microbiology 2024Quote: ... EMVs were obtained by ultracentrifugation from 3 mL of filtrate at 100,000 × g (average centrifugal force) and 4 °C for 2 h with 50Ti rotor and Optimax centrifuge (Beckman Coulter, Brea, CA, United States). The supernatant after removing EMVs was collected as PVF ...
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bioRxiv - Molecular Biology 2024Quote: ... followed by ultracentrifugation for 15 h at 79 000 x g at 4°C in an Optima L-80 XP Ultracentrifuge (Beckman Coulter SW41-Ti rotor). Starting from the top of the gradient ...
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bioRxiv - Cancer Biology 2024Quote: ... Caspase 3 and 8 activity (EGFP+ cells) was analyzed using the CytoFLEX Flow Cytometer (Beckman Coulter) and Flowjo v10 BD (Becton Dickinson ...
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bioRxiv - Biochemistry 2022Quote: ... Around 100 µL of sample at 15 µM was applied to each gradient and centrifuged by ultracentrifugation at 45 000 rpm at 4°C in SW 60 Ti Swinging-bucket rotor (Beckman Coulter, Palo Alto, US-CA) for 16 hours ...
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bioRxiv - Microbiology 2020Quote: ... Prepared phage suspension was transferred to U-formed centrifuge tubes suitable for ultra-centrifugation (25 000 × g, 120 min, 4 °C) (Beckman Coulter, Optima™ MAX-XP). Pellets were resuspended in 200 µL of SM buffer and phage stock suspensions were stored at 4°C and −80°C.
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bioRxiv - Cell Biology 2021Quote: ... This lysate was sonicated for 2 min (15 s on and 20 s off for 2 min at 20% amplitude) and centrifuged at 100,000 x g for 40 min at 4°C (Avanti J-301 Beckman Coulter centrifuge with rotor JA-30.50). The supernatant was filtered and passed through preequilibrated 5 ml HisTrap FF columns (GE Healthcare) ...
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bioRxiv - Biophysics 2022Quote: ... Labeled G-actin was clarified to remove any remaining F-actin or precipitated FMAL by centrifugation (10 min, 90,000 rpm in a Beckman TLA-120.2 rotor at 4°C). The extent of labeling (typically around 70% ...
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bioRxiv - Biochemistry 2024Quote: ... Pellets were washed and gently resuspended in 1 mL of PBS and centrifuged at 50,000 x g for 1 h at 4°C (Beckman Optima MAX-E, TLA-55 rotor). Pellets were resuspended in 500 μl of PBS to obtain concentrated lentiviral stocks that were stored at −80°C in aliquots ...
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bioRxiv - Biochemistry 2023Quote: ... Pellets were washed and gently resuspended in 1 ml of PBS and centrifuged at 50,000 × g for 1 h at 4°C (Beckman Optima MAX-E, TLA-55 rotor). The resulting pellets were resuspended in 500 μl of PBS to obtain concentrated lentiviral stocks that were stored at −80°C in aliquots ...
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bioRxiv - Biophysics 2023Quote: ... Cells were harvested by centrifugation at 8000 g at 4°C for 20 min (Avanti® J-E centrifuge from Beckman Coulter, rotor: JLA 16.250).
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bioRxiv - Bioengineering 2023Quote: ... subtilis spores were collected from the culture by the centrifugation at 8000 rpm at 4 °C for 20 min (Avanti J-E, Beckman Coulter Life Science, IN, USA). Supernatant was removed and the spore pellets were resuspended in fresh 100 mM PBS pH 7.4 ...
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bioRxiv - Microbiology 2024Quote: ... Virus was collected 48 hours post-transfection and pelleted over a 10% sucrose cushion at 25,000 RPM for 2 hours at 4 °C using a SW32Ti rotor (Beckman Coulter Life Sciences, Brea, CA, USA). Pellets were resuspended in DMEM (Gibco ThermoFisher Scientific ...
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bioRxiv - Biophysics 2024Quote: ... The virus was pelleted from supernatants via centrifugation at 14000 rpm for 6 hours at 4°C using a Beckman J2-21 high-speed ultracentrifuge fitted with a JA14 rotor (Beckman Coulter Inc., Fullerton, CA, USA). After carefully removing supernatants ...
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bioRxiv - Neuroscience 2020Quote: ... for further depletion of cell debris and spun at 10,000 × g for 30 min at 4°C (AH-650 rotor, Beckman Ultra-Clear Tube with 5 ml capacity). The pellet was resuspended in 100 μl PBS and considered to be the “10K pellet” (10K or 10K fraction) ...
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bioRxiv - Immunology 2023Quote: ... UC-EVs were obtained by centrifuging 50 mL of conditioned media at 100,000 × g for 70 min at 4 °C (AH-629/36 rotor, Beckman Ultra-Clear Tubes with 36 ml capacity). Pellet was re-suspended in 250 µL of DPBS ...
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bioRxiv - Microbiology 2024Quote: ... The inner and outer membranes were precipitated through a first ultracentrifugation step (107 minutes at 55,000 rpm with a Beckman TLA-100.3 fixed-angle rotor, 4°C). To solubilize the inner membrane ...
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bioRxiv - Microbiology 2021Quote: ... 60% w/v) and centrifuged at 64,000 x g for 3 hours in A27-8×50 mL rotor (Beckman Coulter). The visible virus bands were collected ...
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bioRxiv - Synthetic Biology 2021Quote: ... 8 °C (Beckman Coulter Optima MAX-XP ...
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bioRxiv - Synthetic Biology 2021Quote: ... 8 °C (Beckman Coulter Optima MAX-XP ...
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bioRxiv - Cancer Biology 2024Quote: ... and spinning at 12,535 g for 8 s (Beckman E centrifuge ...
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bioRxiv - Microbiology 2023Quote: ... using a Biomek NXP Span-8 pipetting robot (Beckman Coulter), and pooled and concentrated on PCR purification columns (Analytik Jena) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... using a liquid-handling robot (Biomek NXP Span-8; Beckman Coulter). At each transfer ...
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bioRxiv - Molecular Biology 2020Quote: ... Test PCR was cleaned with 8 μl AMPure XP beads (Beckman A63881) and eluted with 2.5 μl 0.1x TE ...
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bioRxiv - Molecular Biology 2021Quote: ... Test PCR was cleaned with 8 μl AMPure XP beads (Beckman A63881) and eluted with 2.5 μl 0.1x TE ...