Labshake search
Citations for Beckman :
1051 - 1100 of 1258 citations for 3 4 Dehydro Cilostazol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... Vesicles recovered by ultracentrifugation of filtered supernatant at 150,000 x g for 2 h at 4 °C using a Ti 45 rotor (Beckman Instruments, USA), were washed with phosphate-buffered saline (PBS ...
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bioRxiv - Bioengineering 2022Quote: ... Cells were subsequently incubated with 2.5×103 CFSE-RJ-EVs per cell for 4 hours and analyzed with flow cytometry (CyAn ADP, Beckman Coulter, US). Treatment groups were compared to unstained control as well as CFSE-RJ-EVs without inhibitor ...
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bioRxiv - Cell Biology 2022Quote: Whole bovine milk samples (200 mL) were centrifuged at 3,000 x g for 15 min at 4°C (Allegra X-15R, Beckman Coulter, France) to separate the fat from the skimmed milk ...
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bioRxiv - Cell Biology 2022Quote: ... After ultracentrifugation at 200,000 x g for 5 h at 4°C (SW55Ti rotor in a XL-90 Beckman Coulter centrifuge), fractions containing purified EVs or purified phages were extracted from the top following the scheme in Supp ...
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bioRxiv - Biochemistry 2022Quote: ... Soluble and insoluble fractions were isolated via centrifugation (90,000 x g, 30 min, 4 °C) in an Optima™ MAX-OP (Beckman Coulter) centrifuge using the TLA-100.3 rotor ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatant was recovered and centrifuged at 60,000 rpm for 15 min at 4°C in an Optima TL micro-ultracentrifuges (TLA 100.3 rotor Beckman Coulter, Fullerton, CA) to obtain the Golgi-enriched p100 pellet fraction ...
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bioRxiv - Physiology 2022Quote: ... 40 ml of filtrates were ultracentrifuged twice in sterile 10.4 mL polycarbonate bottles at 100 000 x g for 70 min at 4°C using an Optima L-100 XP Ultracentrifuge equipped with 90 Ti Fixed-Angle Titanium Rotor (Beckman Coulter). The final EVs pellets were suspended in 200 µl of PBS (Lonza) ...
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bioRxiv - Microbiology 2022Quote: ... respectively) by centrifugation at 4000 × rpm for 10 min at 4 °C using an Allegra® X-15R centrifuge (Beckman Coulter) and flash-frozen in liquid nitrogen before RNA isolation ...
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bioRxiv - Neuroscience 2022Quote: ... layers F1/2 were removed before transferring the combined F3/4 layer into a clean 25mL centrifuge tube (Beckman Coulter, #355618) and diluting in GM buffer (at least 1:4 ...
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bioRxiv - Neuroscience 2022Quote: ... The discontinuous sucrose gradient was centrifuged at 242K g for 47 min at 4 °C in a vertical rotor (VTi50 rotor, Beckman Coulter). The bulk GC fraction (GCF ...
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bioRxiv - Microbiology 2022Quote: ... Lysates were then submitted to centrifugation at 100,000 g for 45 min at 4°C in an Ultracentrifuge Beckman XL-90 (Beckman France, Villepinte) equipped with a 70.1T1 rotor ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were then spun at 100,000 xg for 16 h at 4°C using a TLS-55 swinging-bucket rotor and benchtop ultracentrifuge (Optima Max-XP, Beckman Coulter). Fractions were collected and loaded in SDS-PAGE for WB.
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bioRxiv - Cancer Biology 2024Quote: ... CRC cells were then cocultured at a 1:1 ratio with BMDCs for 4 hours and analyzed for uptake of CRC DNA by flow cytometry on the CytoFlex S cytometer (Beckman Coulter). Analysis was performed in FlowJo.
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bioRxiv - Biophysics 2024Quote: ... The virions were harvested from the supernatant by centrifugation through 1.5ml of 30% sucrose at 10,000 RPM at 4°C for 2hrs (Beckman L8-70M Ultracentrifuge). The resulting pellet of virions was resuspended in 20ul of RIPA lysis buffer containing protease inhibitors.
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bioRxiv - Molecular Biology 2024Quote: ... for ultracentrifugation at 100,000 rcf for 70 min at 4 °C using a Optima XE-90 ultracentrifuge with a SW 32 Ti rotor (Beckman Coulter). The EV pellet was resuspended in 0.22 μm-filtered sterile PBS and freshly-used or stored at −80 °C until further use ...
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bioRxiv - Neuroscience 2024Quote: ... and centrifuged at ∼14.000 x g max (∼10900 x g average) for 22 min at 4 °C (Beckman Coulter, MAX-XP). After centrifugation ...
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bioRxiv - Cell Biology 2024Quote: ... plasma samples were thawed on ice and subjected to ultracentrifugation at 100,000 g for 1 hr or 18 hr at 4 °C (TLA-55 rotor; Optima MAX-MP Tabletop Ultracentrifuge, Beckman Coulter). Pellets were washed once in 1.0 ml PBS ...
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bioRxiv - Cell Biology 2024Quote: ... The samples were ultracentrifuged at 39,000 rpm at 4°C for 2.5 h in an SW41 rotor (Beckman Coulter, California, USA). Finally ...
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bioRxiv - Microbiology 2024Quote: ... The final Hcp1-VLPs particles were pelletized by ultracentrifugation at 274,000 ×g at 4°C for 1 h in a SW41 Ti rotor (Beckman Coulter, Inc). The final pellet was resuspended in PBS pH 7.4.
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bioRxiv - Microbiology 2023Quote: ... The normalized cultures were then centrifuged at 5030 x g and 4°C for 20 minutes on an Avanti J-26 XPI (Beckman-Coulter) centrifuge (JS 5.3 rotor) ...
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bioRxiv - Microbiology 2024Quote: ... Most of the supernatant was discarded leaving 12x∼200 µL of concentrated broken pieces of genomic fiber that were pooled and layered on top of ultracentrifuge tubes of 4 mL (polypropylene centrifuge tubes, Beckman Coulter) containing a discontinuous cesium chloride gradient (1.4 ...
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bioRxiv - Neuroscience 2023Quote: ... Supernatant were loaded on top of a discontinuous 0.75/1.0/2.33 M sucrose density gradient and centrifuge at 242,000 x g for 60 min at 4°C (Beckman SWTi41 swing rotor). The growth cone depleted fraction was collected between 0.75 M and 1.0 M sucrose ...
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bioRxiv - Cell Biology 2023Quote: ... Homogenates were then transferred to a 50 ml tube and centrifuged for 10 mins at 1 000 × g at 4°C (Beckman Coulter). The resulting supernatant was then transferred to microfuge tubes for storage at -80°C as whole liver lysates or to fresh ultracentrifuge tubes for further processing.
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bioRxiv - Physiology 2023Quote: ... was ultracentrifuged twice in sterile 10.4 mL polycarbonate bottles at 100 000 × g for 70 min at 4°C (Optima L-100 XP Ultracentrifuge, 90 Ti Fixed-Angle Titanium Rotor; Beckman Coulter). The final EV pellet was suspended in 50 µl of PBS without Ca2+ and Mg2+ (Lonza ...
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bioRxiv - Cancer Biology 2023Quote: ... and centrifuged at 100,000 x g either for 4 hr (hollow fiber bioreactor) or overnight (2D flask culture) in a SW32 rotor (Beckman Coulter). For the preparation of the density gradient ...
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bioRxiv - Plant Biology 2023Quote: ... The cAW was collected by spinning at 700 x g for 20 min at 4 ⁰C (JA-14 rotor, Beckman Coulter; Avanti J-20TM centrifuge, Beckman Coulter). vAW was filtered through a single layer of Miracloth and was combined with the cAW ...
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bioRxiv - Cell Biology 2023Quote: ... The cilia in the upper phase were sedimented by centrifugation (27,000 g, 4°C, 20 min; Beckman Coulter, Avanti JXN-26) and resuspended in Hepes-Magnesium-EGTA-Potassium (HMEK ...
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bioRxiv - Biochemistry 2023Quote: ... The sample was run on the gradient for 16 hrs at 4 °C using an Optima XE-90 ultracentrifuge (SW40Ti rotor, Beckman-Coulter) at 30,000 rpm ...
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bioRxiv - Cell Biology 2023Quote: ... and ultracentrifuged at 200,000 × g for 4 hours using a SW 55 Ti rotor in a Beckman OPTIMA90X ultracentrifuge (Beckman Coulter, USA). Finally ...
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bioRxiv - Biophysics 2023Quote: ... the SWCNT suspension was centrifuged at 30000 rpm (164 000 x g) for 4 h at 25 °C (Optima XPN-80, Beckman Coulter) to remove any remaining nanotube aggregates.
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bioRxiv - Genomics 2023Quote: ... cellular/protein debris were then collected by centrifugation at 1,000xg for 15 minutes at 4°C (Beckman Coulter Allegra X-14R), and the supernatant (DNA ...
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bioRxiv - Biophysics 2022Quote: ... EVs were separated from protein aggregates and free reagents by bottom-up density gradient centrifugation in sucrose for 17.30 h at 192,000 g and 4°C using a SW40 rotor (k-factor 144.5; Beckman Coulter, Fullerton, California, USA). Twelve fractions of 1 mL were then collected from the top of the gradient and respective densities were determined by refractometry using an Atago Illuminator (Japan) ...
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bioRxiv - Neuroscience 2024Quote: ... The same mass of total extracts was taken for a high spin at 154,000 g for 20 minutes in 4 °C using an ultracentrifuge (Beckman, TLX-120K) to obtain the supernatant soluble fraction (S1 ...
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bioRxiv - Biochemistry 2023Quote: ... Mitochondrial crude was pelleted by centrifugation at 9500 ×g (4 °C, 15 min) using the Optima XE-90 Ultracentrifuge with a Ti-45 rotor (Beckman Coulter). The final pellet was resuspended in 2 mL of Resuspension buffer (250 mM sucrose ...
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bioRxiv - Bioengineering 2023Quote: ... The gradient tubes were balanced and subjected to centrifugation at 135,000 × g for 2 h at 4 °C using a fixed-angle rotor (TLA-55, Beckman Coulter, USA). After DGUC ...
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bioRxiv - Bioengineering 2023Quote: ... The gradient tubes were subjected to centrifugation at 135,000 × g for 2 h at 4 °C using a fixed-angle rotor (TLA-55, Beckman Coulter, USA). After DGUC ...
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bioRxiv - Bioengineering 2023Quote: ... The suspension was then ultracentrifuged (58,000 × g, 1 hr., 4 °C) in an Optima MAX-XP (Beckman Coulter, Indianapolis, IN, USA) to separate residual catalyst ...
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bioRxiv - Bioengineering 2023Quote: ... The gradient tube was balanced and subjected to ultracentrifugation at 150,000 × g for 2 h at 4 °C using a swinging-bucket rotor (SW 41 Ti Beckman Coulter, USA).
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bioRxiv - Biochemistry 2023Quote: ... then centrifuged at 27,000 gmax for 15 min at 4°C (Optima XPN-100 Ultracentrifuge, Beckman Coulter, with SW60 Ti rotor). Resuspension of the pellet (sub-mitochondrial particles ...
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bioRxiv - Bioengineering 2023Quote: ... Samples were ultracentrifuged at 100,000×g for 70 min at 4°C using the Beckman OptimaTM L-80 XP ultracentrifuge (Beckman Coulter, CA). The supernatant was discarded ...
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bioRxiv - Plant Biology 2024Quote: ... The tubes were then centrifuged for 20 min at 700 x g with slow acceleration at 4°C using a JA-14 rotor (Avanti J-20 XP centrifuge; Beckman Coulter). The resulting apoplastic wash collected was passed through a 0.22 µm membrane filter to remove bacteria and larger debris ...
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bioRxiv - Cell Biology 2024Quote: ... to a volume of ∼12 mL with PBS and separated by ultracentrifugation at 160.000 × g for 2 h (4 °C) in a SW Ti41 rotor (Beckman Coulter #331362). Top-layer LDs were collected by aspiration using a pre-wetted 200 µL pipette tip so that maximal LDs were collected in minimal buffer volume ...
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bioRxiv - Bioengineering 2024Quote: ... we first measured a cell line population in PBS suspension using a coulter counter (Beckman Coulter Multisizer 4, 100 μm aperture) to obtain a cell volume distribution with the standard unit ...
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bioRxiv - Cell Biology 2024Quote: ... We loaded 2 mL of the homogenate mixture on top of a 3.92 ml 62% sucrose in MAB cushion in an Ultra-Clear 5/8” X 4” Beckman Coulter tube (344061, Beckman Coulter). We added the remaining sucrose in MAB solutions ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were resuspended in 100µL of PBS and stored at 4°C in the dark until samples were analyzed on a CytoFLEX LX Flow Cytometer (Beckman Coulter). Data was analyzed using FlowJo® software.
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bioRxiv - Biochemistry 2024Quote: ... Non-solubilized materials were removed by centrifuging the sample at 41,000 r.p.m for 1 h at 4 °C using rotor type 45 Ti in the OptimaTM L-100 XP Ultracentrifuge (BECKMAN COULTER). The resulting supernatant containing the solubilized LysP-GFP was incubated with Ni2+-NTA resin (Qiagen ...
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bioRxiv - Microbiology 2024Quote: ... and labeled intracellularly for the structural HIV-1 p24 protein with the anti-p24 clone KC57 antibody (30 min RT followed by 30 min 4°C, Beckman Coulter). HIV-1 RNA probing was performed using the PrimeFlow RNA Assay (ThermoFisher) ...
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bioRxiv - Microbiology 2024Quote: ... Samples were balanced and centrifuged at 42,000 rpm for 35m at 4°C in a SW55Ti rotor and buckets with tube adaptors (Beckman REF: 356860). The supernatant was then concentrated to 80μl by spinning through Amicon 3kDa MWCO concentrator units at 14,000xg for 15-30m at 4°C ...
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bioRxiv - Microbiology 2024Quote: Bacterial isolates were grown overnight on LB agar and DNA extraction was performed using the GenFind v3 gDNA extraction kit according to standard protocol (Supp. Table 4) (Beckman Coulter). For the mock microbial community ...
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bioRxiv - Biochemistry 2023Quote: ... Colorimetric changes was measured 3 min after mixing the conditioned media with DTNB solution at 450 nm of absorbance (DTX880, Beckman Coulter, Indianapolis, IN, USA). The value from DTNB only was substracted as noise ...