Labshake search
Citations for Beckman :
1001 - 1050 of 1578 citations for Mouse Anti Hepatitis C Virus NS4a b Antibody 1865 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... It was then centrifuged again at 100,000 x g for 1 h at 4 ⁰C (TLA 55 rotor, Beckman Coulter; OptimaTM Max ultracentrifuge, Beckman Coulter). The final pellet containing the purified EV fraction was resuspended in 50 µl of 20 mM Tris-HCl pH 6.0 ...
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bioRxiv - Plant Biology 2023Quote: ... at 10,000 x g for 1 h at 4 ⁰C (JA-17 rotor, Beckman Coulter; Avanti J-20TM centrifuge, Beckman Coulter). The supernatant from this step was decanted into the new tubes and centrifuged at 39,800 x g for 2 h at 4 ⁰C (JA-17 rotor ...
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bioRxiv - Systems Biology 2023Quote: ... The urine was centrifuged at 20,000 x g for 30 minutes at 4°C (k-factor 1,790) in an Optima XPN-80 ultracentrifuge (Beckman Coulter) equipped with a SW32 Ti swinging bucket rotor (Rmin 67 ...
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bioRxiv - Physiology 2023Quote: ... Samples were ultracentrifuged at 100,000 rpm at 16°C for 2 hours (rotor: Beckman TLA-100; Centrifuge: Beckman Optima TLX). 100ul from the top of the tube was collected as VLDL fraction ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were lysed by sonication and the lysate was clarified at 150,000 rcf for 30 min at 4°C using a Type 45 Ti Rotor (Beckman Coulter). The lysate was passed three times though 2mL of pre-equilibrated Ni-NTA Agarose beads (Qiagen ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were lysed by sonication and the lysate was clarified at 150,000 rcf for 30 min at 4°C using a Type 45 Ti Rotor (Beckman Coulter). The lysate was passed three times through 2 mL of pre-equilibrated Ni-NTA Agarose beads (Qiagen ...
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bioRxiv - Molecular Biology 2023Quote: ... The lysate was clarified at 150,000 rcf for 45 min at 4°C using a Type 45 Ti Rotor (Beckman Coulter). The supernatant was incubated with 3 mL IgG Sepharose 6 Fast Flow beads (Cytiva ...
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bioRxiv - Molecular Biology 2023Quote: ... The lysate was clarified at 150,000 rcf for 30 min at 4°C using a Type 45 Ti Rotor (Beckman Coulter). The supernatant was incubated with 3 mL IgG Sepharose 6 Fast Flow beads (Cytiva ...
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bioRxiv - Molecular Biology 2023Quote: ... The lysate was clarified at 503,000 rcf for 45 min at 4°C using a Type 70 Ti Rotor (Beckman Coulter). The supernatant was incubated with 3 mL IgG Sepharose 6 Fast Flow beads (Cytiva ...
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bioRxiv - Cell Biology 2023Quote: ... loaded onto a discontinuous 0.75/1.0/2.33 M sucrose gradient and spin at 242,000 g for 1 h at 4 °C in a SW32Ti rotor (Beckman & Coutler). Growth cone fractions were collected at the interface load/0.75 M sucrose and non-growth cone fractions collected between 0.75/1.0 M sucrose and then analyzed by Western Blot.
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bioRxiv - Microbiology 2023Quote: ... Each tube was centrifuged for 3 hours at 150’000 x g (4°C) using an Optima XPN-80 ultracentrifuge (Beckman Coulter) equipped with a SW 32 Ti swinging bucket (369650 ...
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bioRxiv - Cell Biology 2023Quote: ... The obtained supernatant was layered onto 30 w/v% sucrose solution in ultracentrifugation tubes and subjected to ultracentrifugation at 100,000 × g for 3 h at 4°C using SW32Ti rotor (Beckman courter). The collected sucrose solutions were mixed with PBS and washed by ultracentrifugation at 100,000 × g for 1 h ...
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bioRxiv - Cell Biology 2023Quote: ... The density adjusted FBS was subjected to ultracentrifugation for 25 h at 310,500 x g at 10°C in a 70Ti rotor (Beckman Coulter) before dialysis ...
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bioRxiv - Cell Biology 2023Quote: ... the post-nuclear supernatant was centrifuged for 30 min at 100,000 x g at 4°C using a S120AT2 rotor (Beckman Coulter). The resulting pellet containing the membranes was resuspended in SDS sample buffer (see below).
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bioRxiv - Cell Biology 2023Quote: ... 355647) and centrifuged at 35,000 rpm (100,000xg) for 40min at 4°C (Fixed-angle rotor, Beckman Coulter Optima Max Unltracentrifuge). 100K pellet (sEVs ...
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bioRxiv - Molecular Biology 2023Quote: ... Ribosomes were isolated by sucrose cushion ultracentrifugation at 100,000 rpm at 4°C for 1 h using TLA110 rotor (Beckman Coulter). RNAs in the ribosome pellet were isolated with TRIzol-LS (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2023Quote: ... Approximately 250 µl of crude EV sample resuspended in 20 mM Tris-HCl pH 6.0 was slowly dispensed on top of the column and centrifuged at 100,000 x g for 17 h at 4 ⁰C (SW 41 Ti rotor, Beckman Coulter; OptimaTM L-90K, Beckman Coulter). After the ultracentrifugation ...
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bioRxiv - Plant Biology 2023Quote: ... The resulting solution was then centrifuged again at 39,800 x g for 2 h at 4 ⁰C (JA-17 rotor, Avanti J-20TM centrifuge, Beckman Coulter). The supernatant was decanted ...
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bioRxiv - Plant Biology 2023Quote: ... The supernatant from this step was decanted into the new tubes and centrifuged at 39,800 x g for 2 h at 4 ⁰C (JA-17 rotor, Beckman Coulter; Avanti J-20TM centrifuge, Beckman Coulter). The supernatant was then decanted and discarded ...
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bioRxiv - Plant Biology 2023Quote: ... The tubes were then centrifuged at 100,000 x g for 1 h at 4 ⁰C (TLA 55 rotor, Beckman Coulter; OptimaTM Max ultracentrifuge, Beckman Coulter). The pellets from the tubes were pooled together in 500 µl of 20 mM Tris-HCl pH 6.0 buffer and the volume was brought up to 1,500 µl using 20 mM Tris-HCl ...
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bioRxiv - Biophysics 2023Quote: ... Cell debris and unbroken cells were removed by centrifugation at 2,000 xg for 10 minutes and membranes were collected by ultracentrifugation at 186,000 xg for 1 hour at 4°C with a 45 Ti rotor (Beckman Coulter). The collected membrane was homogenized using a Dounce homogenizer in cold TBS buffer containing 1 mM PMSF ...
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bioRxiv - Neuroscience 2023Quote: ... The gradients were centrifuged for 18 h at 100,000 xg at 4 °C in a SW 40 rotor (Beckman Coulter). 500 µl fractions were collected from the top of the ultracentrifuge tubes and analyzed by WB analysis.
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bioRxiv - Biophysics 2023Quote: SpeMreBs in buffer S polymerized at room temperature with a volume of 200 μL were polymerized for 1–6 h and centrifuged (100,000 rpm at 23 °C for 120 min) in a TLA-100 rotor (Beckman Coulter) (2) ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatant was then transferred to polycarbonate tubes and centrifuged at 100,000 × g and 4°C for 1 h in a XL-90 ultracentrifuge (Beckman Coulter) ...
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bioRxiv - Cell Biology 2022Quote: ... This spin was repeated and the resulting supernatant was then centrifuged for 1 h at 75,000 g at 4°C (TLA-100.3 rotor or Type60 Ti rotor; Beckman Coulter, USA). The supernatant was aspirated and the membrane pellet gently resuspended in microsome buffer (10 mM HEPES/KOH pH 7.5. ...
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bioRxiv - Cell Biology 2022Quote: ... Lysates were cleared by centrifugation for 30 min at 4°C at 18,000 rpm (SS-34 rotor; Beckman-Coulter, USA).
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bioRxiv - Cancer Biology 2023Quote: ... Samples homogenized in lysis buffer were loaded onto a 5%-20% linear gradient and centrifugated at 37,000 rpm for 170 min at 4°C in an SW41Ti rotor (Beckman Coulter).
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bioRxiv - Cell Biology 2023Quote: ... down to 6 ml and ultracentrifuged at 10,000xg for 16 min at 4°C in MLA-80 rotor (Beckman Coulter). The pellet was resuspended and washed with PBS at the same conditions (volume ...
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bioRxiv - Immunology 2023Quote: ... at 950 rpm for 10 min at 20°C and centrifuged at 15,000 rpm (Beckman Coulter Microfuge® 22R refrigerated microcentrifuge, Beckman Coulter Australia Pty Ltd ...
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Comparative membrane proteomics reveals diverse cell regulators concentrated at the nuclear envelopebioRxiv - Cell Biology 2023Quote: ... The whole cell homogenate was layered on top of 2 ml shelf of 0.8 M sucrose in HB and centrifuged at 2000 rpm for 10 min at 4°C in a JS5.2 rotor (Beckman Coulter) to yield a low speed nuclear pellet and a postnuclear supernatant ...
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bioRxiv - Biophysics 2023Quote: Sedimentation velocity experiments of PTPN3-Bidomain were carried out at 20°C using an analytical ultracentrifuge (Beckman Coulter Optima AUC) equipped with a AN60-Ti rotor ...
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bioRxiv - Bioengineering 2023Quote: ... The final supernatant was centrifuged at 100,000g at 4°C for 70 min using an ultracentrifuge (Optima MAX-XP Tabletop Ultracentrifuge, Beckman Coulter). The pellet was either used immediately or stored dry at −80°C.
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bioRxiv - Biochemistry 2023Quote: ... The reaction mixture was applied onto the top of the gradient solution and was centrifuged at 41,000 rpm at 4 °C for 17 hrs using an SW41 Ti rotor (Beckman Coulter). The gradient was fractionated using a Piston Gradient Fractionator (BioComp) ...
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bioRxiv - Cell Biology 2023Quote: ... and then centrifuged for 150 min at 39,000 rpm and 4°C in an Optima L-100XP Ultracentrifuge (Beckman-Coulter) using a SW41Ti rotor ...
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bioRxiv - Cancer Biology 2023Quote: ... Virus particles were collected by centrifugation at 32,000rpm for 2 hours at 4°C using the Optima XE-90 ultracentrifuge (Beckman Coulter). Virus was aliquoted and stored at -80°C until further use ...
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bioRxiv - Biophysics 2023Quote: ... at 4°C until equilibrium was reached using an An Ti 60 rotor in an XL-I analytical ultracentrifuge (Beckman), run using ProteomeLab XL-A/XL-I (version 6.2) ...
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bioRxiv - Microbiology 2023Quote: ... followed by downstream RNA extraction procedures as per manufacturer’s instructions including a DNase treatment step at 37°C for 15 min (Beckman Coulter).
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bioRxiv - Molecular Biology 2023Quote: ... for 45[min at 25°C and then subjected to a sucrose cushion ultracentrifugation at 100,000[rpm for 1[h at 4°C with Optima MAX-TL ultracentrifuge and TLA-110 rotor (Beckman Coulter). The RNA fragments in the resulting pellet were recovered using TRIzol reagent (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... and centrifuged at 100,000 × g for 1 h at 4 °C using a TLA-55 rotor in an Optima TLX Ultracentrifuge (Beckman Coulter). The first supernatant ...
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bioRxiv - Cell Biology 2023Quote: ... The gradient was centrifuged at 4 °C and 100,000 ˗ g for 18 h (Optima L-80 XP Ultracentrifuge with Swing Rotor (Beckman Coulter)) ...
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bioRxiv - Developmental Biology 2023Quote: ... The resulting supernatant was subjected to ultracentrifugation at 120,000 × g at 4°C for 2 h (Optima Max TL, Beckman Coulter). Pellets were resuspended in 1 ml cold PBS and centrifuged at 120,000 × g at 4°C for 2 h ...
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bioRxiv - Developmental Biology 2023Quote: ... The top 1 mL was loaded onto a 52% isotonic Percoll solution and centrifuged at 27,000 × g for 10 min at 10°C (Optima-XPN-80, Beckman Coulter). Following centrifugation ...
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bioRxiv - Immunology 2023Quote: ... and purified and concentrated by ultracentrifugation on a 20% sucrose cushion (24,000 rpm and 4°C for 2 hours with a SW32Ti or SW28 rotor (Beckman Coulter)) ...
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bioRxiv - Pathology 2023Quote: ... The mixture was then layered under a 20–40% discontinuous iodixanol gradient and centrifuged at 260,000 g for 15h at 4°C using an SW 41 Ti Rotor (Beckman Coulter). After spinning ...
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bioRxiv - Neuroscience 2023Quote: ... and samples were centrifuged at 24,000 rpm at 4°C for 1h in a SW41Ti Swinging-Bucket Rotor (Beckman Coulter). After discarding the supernatant ...
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bioRxiv - Cell Biology 2023Quote: ... The samples were sedimented by velocity centrifugation at 36,000 x rpm for 2 h at 4°C using SW40Ti rotor in Optima L-80XP ultracentrifuge (Beckman Coulter). Absorbance at 254 nm was measured from lower to higher sucrose gradients using an ISCO gradient fractionation system and the optical density at 254 nm was continuously recorded with a Foxy JR Fractionator (Teledyne ISCO).
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bioRxiv - Microbiology 2024Quote: ... The resulting gradient was then subjected to centrifugation for 16 hours at 150,000 x g and 4 °C (SW55 Ti rotor, Beckman-Coulter). Sample banding was visually observed at the interface of the 30% and 20% fractions (Supporting Information ...
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bioRxiv - Cell Biology 2024Quote: ... The step gradients were centrifuged for 5 h at 132.000 x g at 4 °C (SW60 Beckman and Coulter, # 335649). Subsequently ...
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bioRxiv - Cell Biology 2024Quote: ... The same amounts of proteins were loaded on a double sucrose cushion (20% and 30% sucrose) and centrifuged at 190,000 g for 2 h at 4 °C in an Optima L-100XP ultracentrifuge (Beckman–Coulter). Extracts were incubated with pre-washed anti-Flag beads (Sigma ...
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bioRxiv - Synthetic Biology 2024Quote: ... The gradients were centrifuged at 154,300× g for 18 hours at 18°C (SW41Ti rotor, Optima XE-90 Ultracentrifuge [Beckman Coulter]). After centrifugation ...