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• The combination of three CD4-induced antibodies targeting highly conserved Env regions with a small CD4-mimetic achieves potent ADCC activity

  Lorie Marchitto, et al., bioRxiv - Microbiology 2024

  Quote: ... was then added to the labelling reaction and incubated for additional 30 min at room temperature before labeled-virus purification through ultracentrifugation for 1 hour at 35,000 RPM using a rotor SW40Ti (Beckman Coulter Life Sciences, Brea, CA, USA), at 4 °C in a 6–30% OptiPrep (Sigma-Aldrich ...
• Fuse to defuse: a self-limiting ribonuclease-ring nuclease fusion for type III CRISPR defence

  Aleksei Samolygo, et al., bioRxiv - Biochemistry 2020

  Quote: ... Protein expression was induced with 0.4 mM Isopropyl β-D-1-thiogalactopyranoside and cells were grown at 37 °C overnight before harvesting by centrifugation at 4000 rpm (Beckman Coulter Avanti JXN-26; JLA8.1 rotor) at 10 °C for 10 min.
• The combination of three CD4-induced antibodies targeting highly conserved Env regions with a small CD4-mimetic achieves potent ADCC activity

  Lorie Marchitto, et al., bioRxiv - Microbiology 2024

  Quote: ... and stained intracellularly using PE-conjugated mouse anti-p24 mAb or using PE of FITC-conjugated mouse anti-p24 mAb (clone KC57; Beckman Coulter, Brea, CA, USA; 1:100 dilution). Cell-surface staining of infected primary MDMs cells was performed five days post-infection ...