Labshake search
Citations for Beckman :
951 - 1000 of 1024 citations for Nonanoic acid reaction products with diethanolamine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2021Quote: ... Double-stranded DNA was purified from the second-strand reaction using 1.8x volume of AMPure XP SPRI beads (Beckman Coulter). As a negative control ...
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bioRxiv - Bioengineering 2021Quote: ... and pooled into 100 µl reactions for Solid Phase Reversible Immobilization (SPRI) cleanup (1X) using AMPure XP beads according to the manufacturer’s instructions (Beckman Coulter). Each 100 µl starting product was eluted into 5 µl of water ...
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bioRxiv - Biochemistry 2020Quote: ... and then the entire reaction was moved to a pre-warmed cuvettes at 37°C in a DU 800 spectrophotometer (Beckman Coulter Life Sciences ...
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bioRxiv - Microbiology 2020Quote: ... water was added to a total volume of 50 μl and reactions were purified using AMPure XP beads (Beckman Coulter) with a 0.9X ratio according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... the absorbance at 620 nm (A620) of the reactions (200 μl each) was measured with a DU720 spectrophotometer (Beckman Coulter). The raw A620 data were corrected by subtracting the A620 values of the control reactions without His12-Arf proteins ...
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bioRxiv - Bioengineering 2020Quote: ... cDNA from each sample was generated with Smart-seq2 protocol using 20 µL total reaction volume and roughly 200 ng RNA input followed by 0.8X AMPure XP beads (Beckman Coulter) purification ...
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bioRxiv - Synthetic Biology 2021Quote: Reactions were assembled in a final volume of 2 μL using a Labcyte Echo® 550 (Beckman Coulter, Brea, CA) via a two-step strategy ...
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bioRxiv - Neuroscience 2020Quote: ... Groups of 8 reactions were pooled to yield ∼250 μL and purified with Ampure XP Beads (0.6x ratio; Beckman Coulter), washed twice with 75% Ethanol ...
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bioRxiv - Molecular Biology 2021Quote: ... The reaction product (in the form of a cDNA:RNA hybrid) was purified with 1.5x reaction volume of AMPure XP beads (Beckman Coulter, A63881) and eluted in 13.5 μl TE buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... The reaction product (in the form of a cDNA:RNA hybrid) was purified with 1.5x reaction volume of AMPure XP beads (Beckman Coulter, A63881) and eluted in 16 μl TE buffer as described above ...
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bioRxiv - Immunology 2021Quote: ... The first-strand synthesis reaction was purified using the Ampure Size Select Magnetic Bead Kit at a ratio of 0.6X (Beckman Coulter). Following ...
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bioRxiv - Immunology 2021Quote: ... The first PCR reaction was purified using the AMPure Size Select Magnetic Bead Kit at a ratio of 0.6X (Beckman Coulter). Amplicon libraries were then prepared according to the Pacific Biosciences Multiplex SMRT Sequencing protocol and sequenced on a Pacific Biosciences Sequel platform (Pacific Biosciences ...
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bioRxiv - Genomics 2021Quote: ... and after cDNA amplification separated the sample as follows: We performed a 0.6X left-sided cleanup reaction with SPRIselect (Beckman Coulter) and collected both the beads (which carry material for preparing the gene expression library ...
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bioRxiv - Plant Biology 2024Quote: ... using a standard protocol but with all reaction volumes reduced by 1/10 to accommodate the automation of the prep on the Echo LabCyte liquid handler (Beckman). Libraries were purified using Agencourt Ampure XP beads (Beckman Coulter) ...
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bioRxiv - Genetics 2023Quote: ... Sub-reactions were mixed and purification was performed using 30 μL AMPure XP magnetic beads (Beckman Coulter; cat. no. A63880) according to manufacturer’s instructions and eluted in 21 μL H2O ...
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bioRxiv - Biochemistry 2023Quote: ... Eight 50 μL PCR reactions were combined in a 1.5 mL Eppendorf tube and mixed with 320 μL Ampure XP beads (Beckman Coulter) (0.8:1 ratio ...
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bioRxiv - Biophysics 2023Quote: ... Reactions were centrifuged at 400,000 g at 20 °C for 15 min in polycarbonate centrifuge tubes (Beckman Coulter Inc. USA). The pellets were resuspended in 40 μl reaction buffer ...
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Faa1 membrane binding drives positive feedback in autophagosome biogenesis via fatty acid activationbioRxiv - Biochemistry 2023Quote: ... The reaction was centrifuged at 68 000 rpm for 15 min at 22 °C (Beckman-Coulter Optima MAX, TLA100 rotor). The supernatant and pellet were loaded separately on an SDS/polyacrylamide gel in equal amounts ...
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bioRxiv - Evolutionary Biology 2023Quote: ... viral cDNA was purified either by standard PCR reaction desalting columns or with a 0.45X volume of AMPure XP beads (Beckman Coulter). Nextera XT libraries were generated with 1 ng of cDNA material ...
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bioRxiv - Microbiology 2024Quote: ... viral cDNA was purified either by standard PCR reaction desalting columns or with a 0.45X volume of AMPure XP beads (Beckman Coulter). Libraries were generated either by Nextera XT with 1 ng of cDNA material ...
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bioRxiv - Biochemistry 2024Quote: ... Reaction mixtures were incubated for 20 minutes at 25 °C then centrifuged at 155,000 x g (TLA-100; Beckman Coulter) for 25 minutes at 25 °C ...
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bioRxiv - Biochemistry 2020Quote: ... was then added to the product which passed microfluidizer and unbroken cells and large debris were eliminated by centrifugation at 4000 × g (Beckman Coulter™, USA). The supernatant containing the IMVs was collected and washed several times with resuspension buffer by centrifugation at approximately 310,000 × g at 4°C for 30 minutes (SORVALL® Centrifuges ...
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bioRxiv - Microbiology 2021Quote: ... Equimolar concentrations of PCR products were purified and pooled using a 0.8 volume of AMPure XP beads (Beckman Coulter Genomics, Danvers, MA, USA) and eluted with molecular water ...
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bioRxiv - Immunology 2021Quote: ... as well as the mRNA PCR product were separated and purified by double-sided size selection using AMPure XP magnetic beads (Cat. No. A63880; Beckman Coulter, Krefeld, Germany). A fraction of the Abseq/multiplex sample tag PCR 1 product ...
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bioRxiv - Physiology 2022Quote: ... the library preparation step followed the CEL-Seq 2 protocol85: the RT product was digested by ExoI and HinFI and purified using AMpure XP beads (Beckman Coulter™, USA). Then ...
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bioRxiv - Genetics 2023Quote: ... and then pooled with 2µL of PCR1 product and reverse indexes using the Biomek I5 liquid handling system (Beckman Coulter, Indianapolis, IN, USA). After pooling of the indexed amplicons on the Biomek ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Junction PCR products were purified with a 0.8x bead to sample volume ratio with AMPure XP Reagent beads (Beckman Coulter cat. no. A63881). Either 10 ng plasmid DNA (input ...
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bioRxiv - Molecular Biology 2020Quote: ... The reaction product (in the form of a cDNA:RNA hybrid) was purified with a 1.5X reaction volume of AMPure XP beads (Beckman Coulter, Cat #A63882) and eluted in 13.5 μl TE buffer ...
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bioRxiv - Biochemistry 2020Quote: ... The reaction volume was adjusted with H2O to 40 μL and then purified with 72 μL AMPure XP beads (Beckman Coulter), eluting into 20 μL of H2O.
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bioRxiv - Immunology 2021Quote: ... GeneDireX 1Kb plus DNA ladder RTU or 100bp DNA ladder H3 RTU) and total reactions were purified using AMPure XP beads (Beckman Coulter) at a 1:1 ratio ...
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bioRxiv - Genetics 2020Quote: ... Reverse crosslinking reactions were incubated 2 h at 65°C and cleaned using Agencourt Ampure XP SPRI beads (Cat #A63880, Beckman Coulter) with a 2x bead:sample ratio ...
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bioRxiv - Developmental Biology 2021Quote: ... PCRs were monitored by qPCR to avoid over-ampliciation and following every PCR reaction the samples were purified using SPRI beads (Beckman Coulter) and libraries were sequenced at 1:10 proportion of the transcriptome library on Illumina’s NovaSeq.
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bioRxiv - Genomics 2020Quote: ... We carried out the first round of PCR for 3 cycles and purified the reactions with Ampure XP beads (Beckman-Coulter). We then carried out a second round of PCR with primers targeting the P5 and P7 sequences only ...
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bioRxiv - Genomics 2020Quote: ... These pooled single-cell libraries were used in an AMpure XP Bead-based Dual Bead Cleanup and Size Selection reaction (Agencourt AMPure XP PCR Purification modified Instructions for Use, Beckman Coulter). In both bead cleanup reactions ...
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bioRxiv - Cancer Biology 2022Quote: ... 50uL Qiagen buffer PB was added to each sample to stop the reaction and DNA was isolated with AMPure XP beads (Beckman Coulter). 15 cycles of PCR were performed with transposed DNA using the dual index primers and NEBNext PCR Master Mix ...
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bioRxiv - Molecular Biology 2023Quote: ... Size selection purifications following the ligation and amplification PCR steps were performed with 0.9× reaction volumes of Agencourt AMPure XP beads (Beckman Coulter A63880). Purified libraries were combined as an 8-sample equimolar pool containing a combination of indexes from 1–12 and sequenced on an Illumina NextSeq on an Illumina NextSeq 2000 on a P2 flow cell (paired-end 75-bp reads).
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bioRxiv - Plant Biology 2024Quote: ... Then the reaction was started by adding 150 µL of a 1 M proline solution and the decrease in the OD600 monitored until at least 1 min of linear reaction was observed (DU-800 Spectrophotometer, Beckman Coulter). Reaction rates were calculated using the 600 nm absorbance coefficient of DCPIP (19.1 mM-1 cm-1 ...
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bioRxiv - Immunology 2024Quote: ... The product of the reverse transcription reaction was purified with AMPure XP beads at a 1:1 bead to sample ratio (Beckman Coulter). Purified cDNA was split in half to amplify the heavy and light chains in separate reactions ...
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bioRxiv - Systems Biology 2023Quote: ... We performed double-sided library purifications on the pooled samples using first 0.6x (30 μL x the number of pooled reactions) AMPure XP magnetic beads (Beckman Coulter, A63881) and keeping the supernatant ...
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bioRxiv - Biophysics 2023Quote: ... The labeling reaction mixture was separated using on a C-18 reverse phase HPLC Column (Beckman Ultrasphere C-18, 4.6 25cm) that was pre-equilibrated with Buffer 1(filtered MilliQ with 0.1% Trifluoroacetic acid (Sigma-Aldrich)) ...
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bioRxiv - Microbiology 2023Quote: ... The remaining PCR reaction of samples treated with a single gRNA was subjected to purification with AMPure XP reagent (Beckman Coulter) according to the manufacturer’s manual using AMPure beads ...
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bioRxiv - Microbiology 2024Quote: ... following a modification of the manufacturer’s protocol: DNA was purified between enzymatic reactions and library size selection was performed with AMPure XP beads (Beckman Coulter Genomics). The library concentrations were measured using the Qubit® dsDNA HS Assay Kit from Molecular Probes (Invitrogen ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Barcoded PCR products (234 bp) were pooled from triplicate tubes and purified by double-sided size selection using Ampure beads (Beckman Coulter, Pasadena, CA, USA). In brief ...
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bioRxiv - Immunology 2022Quote: ... and mRNA PCR products were separated from sample tag and AbSeq products with double-sided size selection using Agentcourt® AMPure® XP magnetic beads (Beckman Coulter, #A63880). mRNA and Sample Tag products were further amplified ...
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bioRxiv - Microbiology 2022Quote: ... The success of the amplifications was checked in a 2% agarose gel and PCR products were subsequently purified using Agencourt AMPure XP magnetic beads kit (Beckman Coulter, Brea, CA, USA), quantified with the QuantiFluor™ dsDNA System (Promega ...
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bioRxiv - Microbiology 2022Quote: ... and (III) the PCR products as the final construction of the libraries were purified with AMPure XP system (Beckman Coulter Inc., Indianapolis, IN, USA). Sequencing library size distribution quality control was performed with an Agilent 2100 Bioanalyzer (Agilent Technologies ...
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bioRxiv - Molecular Biology 2021Quote: ... the resin was spun down by centrifugation and nucleic acids were purified from supernatant with AMPure XP magnetic beads (Beckman Coulter). As a control ...
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bioRxiv - Cancer Biology 2022Quote: ... Nucleic acid size selection to enrich for fragment sizes between 200-500bp was conducted using SPRIselect (Beckman Coulter Life Sciences, B23318). Samples were pooled and sequenced on NextSeq2000 using TruSeq ChIP and Swift Bioscience Accel-NGS 2S Plus DNA Library Prep Kits and paired-end sequencing ...
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bioRxiv - Genetics 2023Quote: ... the cell pellet was resuspended in PBS supplemented with 2 % FCS and nucleated cells were counted in methylene blue with 3 % acetic acid on a Vi-Cell XR cell viability counter (Beckman Coulter). 10 × 106 bone marrow cells were resuspended in 200 μl of PBS supplemented with 2 % FCS containing the following antibody solution ...
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bioRxiv - Biochemistry 2021Quote: ... Binding reactions were spun at 300,000 g for 30 min at 25 °C using Optima MAX-TL ultracentrifuge (Beckman Coulter, Brea, CA). Bundling reactions were spun at 17,000 g for 15 min at 25 °C ...