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Citations for Beckman :
951 - 1000 of 1082 citations for Mouse Muscleblind Like Protein 1 MBNL1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... 20 mL of supernatant was gently layered on top of 10mL of 20% sucrose dissolved in PBS (4°C, sterile filtered) in an ultra-clear 1×3.5′ centrifuge tube (Beckman 344058). The tubes were spun down in a SW28 rotor (Beckman ...
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bioRxiv - Biochemistry 2024Quote: ... and the solution was centrifuged for 45 min at 20,000g at 1 °C (Beckman Coulter J2-21, JA-20 rotor). The supernatant was then loaded onto a 5 ml HisTrap column (Cytiva ...
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bioRxiv - Plant Biology 2020Quote: MP of pollen protoplasts was assessed by staining with 1 µM DiBAC4(3) for 5 min (Breygina et al. 2010) on Gallios flow cytometer (Beckman Coulter), analysis was performed with FlowJo software (TreeStar) ...
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Phenotypic and molecular evolution across 10,000 generations in laboratory budding yeast populationsbioRxiv - Evolutionary Biology 2020Quote: ... We performed daily 1:210 dilutions of populations in YPD 30°C and SC 30°C and daily 1:28 dilutions of populations in SC 37°C using a BiomekFXp robot (Beckman Coulter). Before dilution ...
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bioRxiv - Developmental Biology 2020Quote: ... Subsequently libraries were subject to size selection to exclude DNA fragments larger than 1 kb using SPRIselect magnetic beads (Beckman Coulter). Samples were sequenced on a Illumina HiSeq 4000 platform to obtain 50 bp paired-end reads ...
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bioRxiv - Developmental Biology 2020Quote: ... incubated with fluorophore-conjugated antibodies for 30min (see Supplementary Table 1) and FACS-sorted using MoFlo Legacy or Astrios cell sorters (Beckman Coulter). Cells were sequentially gated based on size and granularity (forward scatter ...
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bioRxiv - Molecular Biology 2022Quote: ... PCR products were analysed by electrophoresis in a 1% (w/v) agarose gel and purified using magnetic AMPure XP beads (Beckman Coulter), using a ratio of beads:PCR product of 2:1 ...
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bioRxiv - Molecular Biology 2020Quote: ... and 1 mM TCEP added before use) and centrifuged for 24 h at 28,000 rpm in a 70Ti rotor (Beckman Coulter, Inc.). The pellets were suspended in washing buffer (20 mM Tris-HCl ...
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bioRxiv - Immunology 2021Quote: ... stained cells were resuspended in 1% paraformaldehyde (PFA) and acquired using Stained cells were acquired on Cytoflex (Beckman Coulter, Brea, CA) and analysed with FlowJo v10.0.8 (Tree Star ...
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bioRxiv - Microbiology 2019Quote: ... Then membranes were pelleted by ultracentrifugation at 49,000 x g for 1 hour using a TLA-100.3 rotor in an Optima TL Ultracentrifuge (Beckman Coulter, Indianapolis, IN), and the supernatant was collected for the soluble fraction ...
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bioRxiv - Cell Biology 2019Quote: The concentration of prey and THP-1 cells were measured prior to phagocytosis by flow cytometry (Accuri C6, BD or CytoFLEX, Beckman-Coulter), if needed ...
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bioRxiv - Biochemistry 2020Quote: ... to remove intact cells and the supernatant was centrifuged again at 25,000 g for 1 hour (Beckman Coulter JA- 20 rotor). The pellet was resuspended in lysis buffer and sodium cholate was added to a final concentration of 1% and stirred at 4°C for 1 hour ...
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bioRxiv - Microbiology 2021Quote: ... the cell suspensions were diluted in 1 mL and loaded onto a cytoflex-LX flow cytometer (Beckman Coulter, Brea, CA, USA) with a 375 nm laser ...
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bioRxiv - Molecular Biology 2020Quote: ... sucrose cushion prepared in PSB with 100 nM CaCl2 and spun for 1 hour at 55,000 rpm (TLS-55 rotor, Beckman Coulter, USA) to remove aggregates ...
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bioRxiv - Microbiology 2019Quote: ... Viruses were then concentrated 50-fold by ultracentrifugation over a sucrose cushion at 23,000RPM for 1 hour at 4C using a SW28 rotor (Beckman Coulter; 342207). Concentrated virus was re-suspended in D10 media ...
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bioRxiv - Cancer Biology 2020Quote: ... The emulsions were then broken using perfluoro-1-octanol and the aqueous fraction was diluted in water and collected for DNA purification with SPRI beads (Beckman Coulter). Sample indexes and Illumina adaptor sequences were then added via a 10 cycle PCR reaction and the amplified material was then SPRI purified a second time ...
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bioRxiv - Microbiology 2022Quote: ... and ½ of each plug was inserted into a well of a 1% LE agarose gel (Beckman Instruments, Inc., Palo Alto, CA) and electrophoresed on a CHEF Mapper® XA Pulsed-Field Gel Electrophoresis System (BioRad ...
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bioRxiv - Plant Biology 2022Quote: ... Thylakoid membranes were extracted via ultracentrifugation at 24,000 rpm for 1 h and 20 min (Beckman Coulter SW 32 Ti rotor). Thylakoids were collected from the step gradient interphases with a Pasteur pipet ...
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bioRxiv - Microbiology 2022Quote: ... The PCR templates were purified using Agencourt AMPure XP (PCR product: AMPure XP beads = 1:0.8; Beckman Coulter, Brea, California, USA) before the second PCR ...
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bioRxiv - Molecular Biology 2022Quote: ... 2D10 cells were washed twice with 1 x phosphate buffered saline (PBS) and analyzed by flow cytometer (Epics Altra, BECKMAN COULTER) for the percentages of cells expressing GFP.
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bioRxiv - Biophysics 2022Quote: ... and the supernatant was subjected to ultracentrifugation to separate the membrane fraction (100,000g, 1 hour, 4°C using an Optima XE-90, Beckman Coulter centrifuge). Membranes were resuspended in lysis buffer supplemented with cOmplete EDTA-free protease inhibitors (Roche) ...
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bioRxiv - Immunology 2022Quote: ... Samples are washed with 1X FACS buffer again to remove the PFA and finally re-suspended in 1 x FACS buffer for flow cytometry analysis using either Fortessa instrument (Becton Dickinson) or CytoFlex LX instrument (Beckman Coulter) fitted with an automated sampler accommodating 96-well plates ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were then enriched in DNA sizes between 200 and 600 bp (corresponding to 1 to 3 nucleosomes) using AMPure XP magnetic beads (Beckman-Coulter), following the manufacturer’s guidelines ...
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bioRxiv - Molecular Biology 2022Quote: Procyclic form cell lines for depletion of either TbPH1 or TbKifX2A were induced at a density of 2×106 cells/ml with 1 µg/ml doxycycline and growth monitored daily with a Z2 Coulter Counter Analyzer (Beckman Coulter). Cells were diluted back to the initial cell density every day ...
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bioRxiv - Cell Biology 2022Quote: ... A new supernatant was collected in a prechilled 1.5 ml ultracentrifuge tube and centrifuged at 100000 g for 1 h at 4°C in a TLA-55 rotor (Beckman Coulter). The resulting pellet was resuspended in the same isotonic buffer containing antiproteases and was frozen at -80°C together with the 100000 g supernatant ...
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bioRxiv - Cell Biology 2019Quote: ... apoptotic and necrotic cells with YO-PRO-1 / PI in parallel with a Annexin V / PI staining realized with the Annnexin-V-FITC when needed (Immunotech, Beckman-Coulter). All samples were measured and analyzed on a FACS Calibur 4C as previously described (23 ...
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bioRxiv - Genetics 2022Quote: ... A final DNase treatment was performed in 50 μL total volume using 1 μL of Turbo DNase incubated for 4 hours at 37°C followed by inactivation with 1 μL of 10% SDS and purification using RNA Ampure XP beads (Beckman Coulter).
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bioRxiv - Cell Biology 2022Quote: Live Jurkat E6.1 cells were stained with Laurdan dye as detailed above and analyzed by flow cytometry using a Cytoflex S (Beckman Coulter). Cells were resuspended in HBSS without Ca2+ and Mg2+ (Gibco ...
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bioRxiv - Cell Biology 2019Quote: ... apoptotic and necrotic cells with YO-PRO-1 / PI in parallel with a Annexin-V / PI staining realized with the Annnexin-V-FITC when needed (Immunotech, Beckman-Coulter). All samples were measured and analyzed on a FACS Calibur 4C as previously described (73 ...
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bioRxiv - Microbiology 2019Quote: ... The concentrated vesicles were washed twice with PBS by ultracentrifugation at 110,000xg at 4°C for 1 hour (Beckman Optma MAX-XP Ultracentrifuge, rotor TLA 120.2; Beckman Coulter Inc., USA). The final pellet (exosomes ...
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bioRxiv - Immunology 2019Quote: ... cells were washed twice with permeabilization buffer and dissolved in FACS buffer (PBS, 1% BSA, 2mM EDTA) for analysis using a CytoFLEX S flow cytometer (Beckman Coulter).
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bioRxiv - Developmental Biology 2020Quote: ... Exosome pellets were resuspended in phosphate-buffered saline (PBS) and centrifuged at 27300 rpm for 1 hour (SW60Ti rotor; Beckman Coulter). The exosome pellets were resuspended in PBS ...
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bioRxiv - Synthetic Biology 2021Quote: ... The complete NanoTrap was purified using rate-zonal centrifugation33 through a 1545% glycerol gradient in 1×TE + 10 mM MgCl2 in an SW 55 rotor (Beckman Coulter). Fractions were collected after a 1 hr centrifugation at 50 k rpm ...
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bioRxiv - Immunology 2019Quote: ... CECs were pre-incubated with human recombinant CCL5 (1-1000 ng/ml, R&D) and/or CD35 blocking antibody (5-20 μg/ml, clone J3D3, Beckman Coulter) for an hour prior to infection.
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bioRxiv - Systems Biology 2020Quote: ... each resultant fraction underwent separate ultracentrifugation at 100,000xg for 40 minutes at 4°C (rotor TLA 120.2) in 1 ml 11×34 mm polycarbonate uncapped centrifuge tubes (Beckman Coulter 343778). In EV-enriched pooled fraction experiments ...
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bioRxiv - Neuroscience 2019Quote: ... Catalog L3224A) and lack of EtD-1 homodimer staining (L3224B) were selected and sorted by a MoFlo Astrios cell sorter (Beckman Coulter) into 500 µL filled into dissecting medium.
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bioRxiv - Synthetic Biology 2020Quote: ... Cells were then filtered through 40 μm Falcon™ Cell Strainers (Thermo Fischer Scientific, cat no. 08-771-1) and analyzed by flow cytometry (MACSQuant VYB, Miltenyi or CytoFLEX, Beckman Coulter). Matlab-based software for processing flow cytometry data was developed in-house by Yaron Antebi.
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bioRxiv - Cancer Biology 2021Quote: ... the 197 library compounds dissolved in DMSO or MilliQ (Supplementary Table 1) were added in duplicate using the Echo 550 dispenser (Beckman Coulter). Final drug concentrations ranged standard between 0.1 nM and 10 μM ...
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bioRxiv - Biochemistry 2021Quote: ... live cells were stained with Alexa 647-conjugated M1 anti-FLAG antibody (1:1000, 5 minutes) and analyzed on a Cytoflex flow cytometer (Beckman Coulter).
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bioRxiv - Biochemistry 2021Quote: ... to remove intact cells and the supernatant was centrifuged again at 25,000 RCF for 1 hour (Beckman Coulter JA-20 rotor). The pellet was resuspended in lysis buffer and sodium cholate was added to a final concentration of 1% and stirred at 4°C for 1 hour ...
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bioRxiv - Microbiology 2020Quote: ... Vesicles were obtained by centrifugation at 38,400 x g for 1 h at 4°C in an Avanti J-20XP centrifuge (Beckman Coulter, Inc). Pelleted vesicles were resuspended in 20 ml of 50 mM HEPES (pH 6.8 ...
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bioRxiv - Microbiology 2020Quote: ... cells were fixed with 4% FA in PBS for 90 min at room temperature and immunostained for intracellular HIV-1 CA for 30 min at 4°C using KC57-FITC antibody (Beckman Coulter) diluted in 0.1% Triton X-100 ...
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bioRxiv - Microbiology 2020Quote: ... the 4 tubes were centrifuged using JA-20 rotor at 43,000 × g for 1 hour and 30 minutes in Beckman coulter® Avanti j-26 xp (Beckman, France). The pellets of the 4 tubes were preserved at −80°C before transport and metagenome sequencing and analysis.
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bioRxiv - Immunology 2021Quote: ... The PBMC were resuspended in 1 mL cold PBS+BSA per 15 mL whole blood processed and counted with a ViCell (Beckman Coulter) using VersaLyse reagent (Beckman Coulter #A09777 ...
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bioRxiv - Immunology 2021Quote: ... 0.1 ng cDNA was used per sample and the libraries were cleaned up with 1.8x AMPure® XP beads (Beckman Coulter). Quality and integrity of nucleic acids were assessed using the Agilent Technologies 2100 Bioanalyzer (Agilent Technologies ...
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bioRxiv - Immunology 2021Quote: ... either 150 μl or a maximum of 1 × 106 viable lymphocytes were analyzed using a CytoFLEX LX Flow Cytometer (Beckman Coulter), and data was analyzed with FlowJo software (FlowJo LCC ...
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bioRxiv - Genetics 2019Quote: ... The PCR products were examined on either 3% agarose gel by electrophoresis at 130 V for 1 hour or using capillary electrophoresis on an automated CEQ2000 system (Beckman-Coulter) as described previously (Raman et al. ...
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bioRxiv - Biochemistry 2022Quote: ... to remove intact cells and the supernatant was centrifuged again at 25,000 RCF for 1 hour (Beckman Coulter JA-20 rotor). The pellet was resuspended in lysis buffer and sodium cholate was added to a final concentration of 1% and stirred at 4°C for 1 hour ...
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bioRxiv - Microbiology 2023Quote: ... and permeabilized in PBS/1% BSA/0.1% saponin before staining with a FITC-conjugated anti-Cap24 (KC57-FITC, Beckman Coulter, France) for 1h at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... 200 μl of the PCR reaction (roughly corresponding to 1 μg of amplicon) was then purified using a double-sided selection with AMpure XP beads (Beckman Coulter). First ...