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Citations for Beckman :
951 - 1000 of 2183 citations for 6H 1 3 Thiazin 6 one 2 ethoxy 4 hydroxy 5 nitro 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2020Quote: ... We carried out the first round of PCR for 3 cycles and purified the reactions with Ampure XP beads (Beckman-Coulter). We then carried out a second round of PCR with primers targeting the P5 and P7 sequences only ...
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bioRxiv - Bioengineering 2022Quote: ... Supernatant (33 mL) was placed on a 3 mL 25% (w/v) sucrose pad in SW28 tube (Beckman, Brea, CA, USA) and centrifuged at 24,000 rpm ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were harvested after 3 days and counted using a Z2 Coulter Particle Count and Size Analyzer (Beckman Coulter, Brea, CA).
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bioRxiv - Cancer Biology 2024Quote: ... The percentage of fluorescence-positive cells was determined initially (baseline reference, passage 0, 3 days after transduction) and all following passages using the CytoFLEX Flow Cytometer (Beckman Coulter). In each passage ...
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bioRxiv - Cell Biology 2023Quote: ... 30% Percoll (v/v)] followed by 3 mL of MRB in a 13 mL ultracentrifuge tube (Beckman Instruments, Inc, CA 331372) and spun at 95,000 x g for 45 min in a TH641 rotor ...
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bioRxiv - Genetics 2023Quote: ... the cell pellet was resuspended in PBS supplemented with 2 % FCS and nucleated cells were counted in methylene blue with 3 % acetic acid on a Vi-Cell XR cell viability counter (Beckman Coulter). 10 × 106 bone marrow cells were resuspended in 200 μl of PBS supplemented with 2 % FCS containing the following antibody solution ...
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bioRxiv - Cancer Biology 2023Quote: Counting of CB CD34+ cells after 3 days of ex vivo co-culture was accurately obtained with flow-count fluorosphere beads (Beckman Coulter), in combination with the following antibodies ...
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bioRxiv - Cell Biology 2021Quote: ... The seeds were sedimented by centrifugation at 90,000 rpm for 5 min at 25 °C (Beckman Coulter). The microtubule pellet was resuspended in warm BRB80 buffer and microtubule seeds were stored in the dark at room temperature.
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bioRxiv - Biochemistry 2022Quote: ... RNA was purified by denaturing gel electrophoresis (5% polyacrylamide) and by AMPure XP magnetic beads (Beckman Coulter). The purified RNA was buffer exchanged three times into RNase-free water using 30 kDa cutoff Amicon centrifugal filters (Millipore) ...
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bioRxiv - Cell Biology 2023Quote: ... The liposome solutions were transferred to 5 × 41 mm ultracentrifuge tubes (Beckman Coulter, CA, USA; Cat. #344090). Two millimolar liposomes were mixed with the indicated concentration of proteins to a total volume of 150 µL ...
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bioRxiv - Immunology 2024Quote: ... mApple-positive cells were sorted 5 days after infection on a Moflo XDP flow cytometer (Beckman Coulter) to obtain mApple-Mem-expressing Nalm6 cell lines.
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bioRxiv - Cell Biology 2024Quote: ... The sample was transferred to a 250 µL ultracentrifuge tube (5 x 20 mm Beckman-Coulter, #342630) and a 50 µL of 10% sucrose cushion was placed below the sample at the bottom of the tube ...
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bioRxiv - Biochemistry 2024Quote: ... samples were ultracentrifuged at 100,000 g for 5 minutes at 25°C (Beckman TL100, rotor TL-A100). Supernatants were recovered and the hexapeptide concentrations of were determined by UV absorption as previously described ...
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bioRxiv - Biophysics 2021Quote: ... Cell debris was removed by centrifugation (12,000x□g for 15□min at 4°C) and membranes were then pelleted at 45,000 rpm in a Type 45 Ti rotor (Beckman) for 3□h at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... Lysates were cleared by ultracentrifugation (40 000 rpm for 30 min at 4°C in a Beckman Ti45 rotor). Supernatant was filtered (0.45 μm ...
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bioRxiv - Biochemistry 2020Quote: ... Lysates were cleared by ultracentrifugation (25 000 rpm for 30 min at 4°C in a Beckman Ti45 rotor). Supernatant was incubated with GSH beads (GE Healthcare ...
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bioRxiv - Biochemistry 2021Quote: ... after which the lysate was clarified by centrifugation at 30,000 x g for 60 min at 4 °C in a JA 25.50 rotor in an Avanti J-E centrifuge (Beckman). Nucleic acids in the clarified lysate were precipitated by slowly adding polyethyleneimine with constant stirring in an ice bath to a final concentration of 0.4% ...
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bioRxiv - Microbiology 2020Quote: ... of 1.5 mL and then centrifuged 45 min at 100,000g at 4°C in a TLA-100 ultracentrifuge (Beckman Coulter). The supernatant contains the soluble fraction and the pellet corresponds to the total membranes ...
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bioRxiv - Cell Biology 2019Quote: ... The resulting gradients were subjected to ultracentrifugation at 4°C for 14 hrs at 41,000 rpm in an SW41 Ti rotor (Beckman), and then fractionated ...
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bioRxiv - Cell Biology 2020Quote: ... 0.5 mM DTT, 100 μg/ml cycloheximide) and centrifuged (160,000 g, 120 min, 4°C) in an SW41Ti rotor (Beckman). After centrifugation the sediment in the tube went through a UV detector at the wavelength of 254nm and simultaneously the required fractions (>=5 ribosomes ...
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bioRxiv - Biophysics 2022Quote: ... The sample was loaded on top of the glycerol gradient and centrifuged at 4°C for 18 h at a speed of 35,000 rpm in SW-41Ti rotor (Beckman). Fractions of 500 μL were collected ...
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bioRxiv - Microbiology 2020Quote: ... Phage were pelleted by centrifugation at 82705 xg (25,000 rpm) for 4 hours using an SW28Ti rotor (Beckman Coulter). Phage pellets were gently resuspended overnight in 1 mL of phage buffer at 4°C with shaking ...
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bioRxiv - Genomics 2021Quote: ... we filtered the virus-laden media through a 0.22μm PES filter and then concentrated viral particles by ultracentrifugation at 24,000 rpm for 1.5 hours (4°C) with an SW-32Ti swinging bucket rotor (Beckman Coulter). We resuspended the viral pellet in iPS-Brew at 1/200th the original supernatant volume and then stored 50-100 μl aliquots at −80°C.
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bioRxiv - Microbiology 2021Quote: ... 200mM NaCl was used to top up the 13mL polyethylene tube and the solutions were spun at 27,000×g for 18 hours at 4°C using SW45 Ti rotor (Beckman). 1mL of top fraction ...
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bioRxiv - Biochemistry 2020Quote: ... and were centrifuged for 4 min at 46 000 rpm at 4°C in a Optima MAX-XP Ultracentrifuge with a TLA 100.3 rotor (Beckman). The pellet was suspended in (20 µl ...
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bioRxiv - Biophysics 2021Quote: ... sucrose density gradients and centrifuged at 24,000 × rpm (102,170 × g) for 18 h at 4°C (Sw40Ti rotor, Beckman Coulter). The fractions of the sucrose gradient were applied to SDS-PAGE ...
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bioRxiv - Biochemistry 2020Quote: ... mixed for 10 minutes at 4°C and centrifuged at 25,000 rpm for 45 minutes (Beckman Ti-45 rotor). The supernatant was warmed to 37°C for few minutes until it turned cloudy following which it was centrifuged at 11,000 rpm at room temperature for 10 minutes (Beckman JA-20 rotor ...
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bioRxiv - Biochemistry 2020Quote: ... Cell debris was removed by centrifugation at 14,800 rpm for 20 minutes 4 °C in F241.5P rotor using 149 Microfuge 22R centrifuge (Beckman Coulter). The supernatant was combined with 100 μL of ANTI-FLAG M2 Affinity Gel (Sigma ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were collected by centrifugation (8 000 rpm for 10 min at 4 °C, JLA-16.25 Beckman Coulter rotor), pellets frozen in liquid nitrogen and stored at −80°C ...
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bioRxiv - Microbiology 2020Quote: ... supernatants were harvested and filtered through a 0.45μm filter and centrifuged at 27,000 rpm for 3h at 4°C with a SW41 rotor and Optima L-90 centrifuge (Beckman). Pellets were resuspended in PBS prior to use for western blot analysis.
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bioRxiv - Microbiology 2020Quote: ... The gradient was centrifuged for 17 h at 4°C and 100,000 rcf (23,700 rpm) using an SW 40 Ti rotor (Beckman Coulter), followed by manual fractionation into 20 590 μl fractions and measurement of the A260 nm of each fraction ...
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bioRxiv - Microbiology 2020Quote: ... the 100,000xg centrifugation was performed for 1h at 4°C using a SW41 TI swinging bucket rotor (Beckman Coulter). The pellet was then resuspended in 50 μL or 1500 μL of D-MEM for subsequent analysis.
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bioRxiv - Neuroscience 2019Quote: ... of the lysates were loaded on top of the gradients and spun at 36,000 rpm at 4°C for 2h with a SW41-Ti rotor (Beckman). Fractions from each sample were collected every 9 sec using a density gradient fractionation system (Teledyne Isco ...
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bioRxiv - Microbiology 2021Quote: ... Filtered supernatants were pelleted at 60,000 g for 16 hours at 4 °C in a SW28 Ti rotor (Beckman), and the resulting pellet was resuspended in TNE buffer (50 mM Tris-HCl [pH 7.4] ...
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bioRxiv - Molecular Biology 2020Quote: ... The reactions were then spun for 20 min at 120,000 rpm at 4°C (TLA120.1 rotor, Beckman Coulter, USA) to remove aggregates and ribosomes ...
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bioRxiv - Microbiology 2020Quote: ... Supernatant was transferred to a fresh tube and further clarified by centrifugation at 18,000 x g for 10 min at 4°C in a microfuge (Beckman), and supernatant transferred to a fresh tube.
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bioRxiv - Microbiology 2020Quote: ... Cell lysate was then clarified 200 x g for 10 min at 4°C in an Allegra centrifuge (Beckman). Supernatant was transferred to a fresh tube and further clarified by centrifugation at 18,000 x g for 10 min at 4°C in a microfuge (Beckman) ...
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bioRxiv - Cell Biology 2022Quote: ... The lysate was clarified by centrifugation (183,960 x g, 88 min, 4°C) in a Type 70Ti rotor (Beckman). The supernatant was mixed with 2 ml of IgG Sepharose 6 Fast Flow beads (GE Healthcare Life Sciences ...
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bioRxiv - Cell Biology 2022Quote: ... The lysate was clarified via centrifugation (66,000 x g, 30 min, 4°C) in a Type 70 Ti rotor (Beckman). The supernatant was mixed with 1.5 ml of anti-FLAG M2 affinity gel (Sigma-Aldrich ...
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bioRxiv - Microbiology 2022Quote: ... sonicated using a sonicator (Smurt, Microtec) and ultracentrifuged at 100,000 x g for 60 minutes at 4°C (Optima XE-90, Beckman). Protein concentration of the bacterial membrane suspension was determined with Bio-Rad protein assay (BioRad).
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bioRxiv - Cell Biology 2022Quote: ... and centrifuged at 10,000g and 4°C for 30 minutes (SW41 rotor, Optima XPN-100 ultracentrifuge; Beckman Coulter, Inc.) to remove any potential large debris ...
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bioRxiv - Biochemistry 2022Quote: ... 5 mM MgCl 2 and 2 mM DTT and centrifuged at 70,000 x g for 20.5 h at 4 °C in an SW28 rotor (Beckman Coulter). Fractions corresponding to the 40S peak were collected ...
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bioRxiv - Biochemistry 2022Quote: ... Gradients were centrifuged at 42,800 x g for 15 h at 4 °C in an SW28 rotor (Beckman Coulter). Fractions of 1 mL were collected using an AKTA system (Cytiva) ...
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bioRxiv - Microbiology 2022Quote: ... at 4°C for 1.5-2h followed by 180,000 x g (Ti 70i rotor; Beckman Instruments, Inc., Fullerton, CA) at 4°C for 1.5-2h and resuspended in 1mL of phosphate buffered saline (PBS ...
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bioRxiv - Genetics 2022Quote: ... The lysate was centrifuged at 2000 rpm for 10 min at 4 °C in a JA-20 rotor (Beckman). The supernatant from the previous step was centrifuged in a Beckman Coulter high speed centrifuge equipped with a JA-20 rotor at 6000 rpm for 20 min at 4 °C ...
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bioRxiv - Neuroscience 2020Quote: ... The gradient was centrifuged at 200,000 × g for 20 h at 4°C (35,000 rpm with # Optima-XE SW41 Beckman Coulter). The gradient was collected in 2-mL fractions ...
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bioRxiv - Neuroscience 2020Quote: ... this second supernatant was centrifuged for 4 hrs at 100,000g (Optima MAX-XP ultracentrifuge, TLA-100.3 rotor, Beckman Coulter). Following ultracentrifugation ...
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bioRxiv - Plant Biology 2020Quote: ... and centrifuged at 131,500 × g for 30 min at 4 °C using a SW 32 Ti rotor (Beckman Coulter). Thylakoid membranes sedimented in 1.3 M sucrose cushion were collected and washed with 25 mM Tricine-KOH (pH 7.8) ...
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bioRxiv - Biochemistry 2022Quote: ... mixed for 10 minutes at 4°C and centrifuged at 25,000 rpm for 45 minutes (Beckman Ti-45 rotor). The supernatant was warmed to 37°C for few minutes until it turned cloudy following which it was centrifuged at 11,000 rpm at room temperature for 10 minutes (Beckman JA-20 rotor ...
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bioRxiv - Systems Biology 2020Quote: ... the 500xg supernatant from enzymatic digestion of cardiovascular tissues underwent further ultracentrifugation at 10,000xg for 10 minutes at 4°C (Beckman Coulter ...