Labshake search
Citations for Beckman :
951 - 1000 of 1005 citations for 6 Cyano imidazo 1 2 a pyridine 2 carboxylic acid ethyl ester since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biophysics 2020Quote: ... The lysate was then centrifuged at 4° C in the SW32Ti rotor with a speed of 30,000 rpm for 1 h to pellet the membrane fraction using an Optima L-90K Ultracentrifuge (Beckman Instruments, Brea, CA). The pellet was resuspended in HND buffer and bulk membrane proteins were extracted by adding 2% ANAPOE-X-100 (Anatrace ...
-
Hydrogen sulfide blocks HIV rebound by maintaining mitochondrial bioenergetics and redox homeostasisbioRxiv - Microbiology 2021Quote: ... Permeabilized cells were then incubated with 50 μl of 1:100 dilution of phycoerythrin (PE)-conjugated mouse anti-p24 monoclonal antibody (KC57-RD1; Beckman Coulter, Inc.) for 30 min at room temperature ...
-
bioRxiv - Cell Biology 2020Quote: ... anti-CD105 and anti-HLA-ABC antibodies (suppl. Table 1) and analysed by flow cytometry (Cytoflex; Software Cytexpert 2.3, Beckman-Coulter, Krefeld, Germany). Upon passage 3 ...
-
bioRxiv - Plant Biology 2020Quote: ... Double size selection was carried out by adding 50 μL of a 1:4 (0.5X) mixture of AMPure XP Beads (Beckman Coulter, Brea, USA) to PEG buffer (20% PEG w/v ...
-
bioRxiv - Plant Biology 2020Quote: ... Soil pH and EC were 8.3 and 12 dS m−1 that were measured by a pH meter (Beckman 45 Modal, US) and EC meter (VWR Conductivity Meter DIG2052 ...
-
bioRxiv - Microbiology 2019Quote: ... Permeabilized cells were then incubated with 100 μl of 1:100 dilution of phycoerythrin-conjugated mouse anti-p24 mAb (KC57-RD1; Beckman Coulter, Inc.) for 30 min at 4 °C with intermittent mixing ...
-
bioRxiv - Microbiology 2020Quote: ... Membrane-containing fractions were collected in 1 mL after ultracentrifugation at 35,000 rpm on an SW-40 rotor (Beckman, Brea, CA USA) for 6 hours at 4°C and stored at -80°C prior to analysis.
-
bioRxiv - Evolutionary Biology 2021Quote: ... with NEBNext Multiplex Oligos for Illumina® (Dual Index Primers Set 1) and AMPure XP magnetic beads (Beckman Coulter, Brea, CA, USA). DNA content and quality of indexed libraries were examined with a Qubit 4.0 using the high-sensitivity kit (ThermoFisher Scientific ...
-
bioRxiv - Microbiology 2019Quote: ... in order to achieve a starting density of 1.70 g mL-1 in a 3.3 mL polyallomer OptiSeal tubes (Beckman Coulter, Brea, CA, USA). After ultracentrifugation ...
-
bioRxiv - Microbiology 2022Quote: ... Cells were permeabilized in 0.5% Triton-X for 10 min and stained with 1:300 KC57-FITC (Beckman Coulter 6604665; RRID: AB_1575987) or 1:300 KC57-RD1 (numbers) ...
-
bioRxiv - Molecular Biology 2023Quote: ... The cell lysate was centrifuged at 4 °C at 35000 rpm for 1 hour with Beckman Optima™ L-80XP Ultracentrifuge equipped with Ti45 rotor (Beckman, USA). The pellet containing membranes and cell debris was discarded ...
-
bioRxiv - Neuroscience 2022Quote: ... The loaded gradient was ultracentrifuged at 69,150 x g for 1 h at 4 °C (SW 41 Ti Swinging-Bucket Rotor, Beckman Coulter, Cat. #331362) and the synaptosome fraction was harvested from the interface between the two sucrose layers ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... and libraries were purified and size selected using Ampure XP Beads at a 1.8:1 bead volume:library volume (Beckman Coulter, Brea, CA, USA). Library quality and transposition efficiency was accessed using a Fragment Analyzer and PROSize 2.0 (Agilent ...
-
bioRxiv - Microbiology 2024Quote: ... Liposomes and liposome-protein complexes were separated from unbound protein by ultracentrifugation of the reaction mixture in 5 mL Optiprep™ (Axis-Shield PoC AS) density gradients for 1 hour at 40,000 ×g (SW55Ti Beckman Coulter rotor). The liposome-protein mixture was adjusted to 34% Optiprep concentration in a volume of 300 μl ...
-
bioRxiv - Microbiology 2023Quote: ... Cell pellets were permeabilized for intracellular staining via resuspension in 0.5% Triton X-100 in PBS for 10 min and were subsequently resuspended in a 1:500 dilution of anti-p24-FITC KC57 antibody (Beckman Coulter #6604665) in 1% BSA in PBS for 1 h at room temperature in the dark ...
-
bioRxiv - Molecular Biology 2022Quote: ... The percentage of live/dead cells was determined within 1 hour of staining by flow cytometry (CytoFLEX, Beckman Coulter, Indianapolis, IN, USA). 10,000 events were collected per sample ...
-
bioRxiv - Cell Biology 2023Quote: ... the supernatants were transferred into SW60 tubes (in vivo studies 1–4) or SW40 tubes (donated patients) (Beckman Coulter Inc., Brea, USA) and centrifuged at 10,000xg for 35 minutes ...
-
bioRxiv - Cell Biology 2023Quote: ... Supernatants were loaded onto a 1 M sucrose cushion and then pelleted by ultracentrifugation by using a TLA100.3 rotor (Beckman Coulter, cat#349490) for 1 hour at 100,000 rpm at 4°C ...
-
bioRxiv - Physiology 2023Quote: ... via centrifugation in a SW41 rotor at 42,000 g at 4 °C for 1 hour in a Beckman Coulter Optima L-100 XP ultracentrifuge using 14 × 89 mm Ultra-Clear Centrifuge Tubes (Beckman Instruments Inc.). Mitochondria were harvested at the interphase between 19 and 40% Percoll ...
-
bioRxiv - Cancer Biology 2024Quote: ... The cells mixed with polybrene and lentivirus were then plated in 1 mL aliquots onto 12 well plates and centrifuged at 2000 rpm (931 x g) [Allegra® X-15R Centrifuge (Beckman Coulter), rotor SX4750A] for 2 hours at 30°C ...
-
bioRxiv - Neuroscience 2024Quote: ... The supernatant was segregated subsequent to centrifuging the brain tissue homogenate for 1 h at 100,000g and 4°C utilising an ultra-high-speed centrifuge (Beckman, Optima MAX-XP). The tissue residue was then reprocessed ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... Then 0.1 μL of library compound (1 mM) dissolved in DMSO was added using pintool integrated with Beckman NX (Beckman Coulter, Brea, CA). The final compound concentration was 20 μM and the final DMSO concentration was 2% ...
-
bioRxiv - Microbiology 2024Quote: ... and stained intracellularly using PE or FITC-conjugated mouse anti-p24 mAb (clone KC57; Beckman Coulter, Brea, CA, USA; 1:100 dilution) Productively-infected cells were identified based on p24 and CD4 detection as described above ...
-
bioRxiv - Microbiology 2024Quote: ... and stained intracellularly using PE or FITC-conjugated mouse anti-p24 mAb (clone KC57; Beckman Coulter, Brea, CA, USA; 1:100 dilution). The percentage of infected cells (p24+ ...
-
bioRxiv - Pathology 2019Quote: ... PCR products were verified using gel electrophoresis (1% agarose gel) and cleaned with AMPure XP beads (Agencourt, Beckman Coulter Inc., Pasadena, CA, USA). Amplicons were then indexed using the Nextera XT Index Kit V2 set A and set D (Illumina ...
-
bioRxiv - Immunology 2021Quote: Immune cell types in Cohort 1 were measured from whole blood by 10-color flow cytometry with Navios flow cytometer (Beckman Coulter, CA, USA). Staining and gating strategies were previously described in detail by Aguirre-Gamboa et al ...
-
bioRxiv - Biochemistry 2020Quote: ... The sample was centrifuged at 4°C for 1 hour and 40 minutes at 182,753 x g (38,000 rpm in a Beckman Coulter Type 42.2 Ti rotor).
-
bioRxiv - Biochemistry 2020Quote: ... The labeled and unlabeled polymerized actin samples were pelleted down by ultracentrifugation at room temperature (45000 rpm for 1 hour, Beckman TLA-55 rotor). The pellets were rinsed once with 1 mL G-buffer and re-suspended into ice cold 0.5 mL G-buffer ...
-
bioRxiv - Molecular Biology 2021Quote: ... 1 mM ATP and 1 mM PMSF) and centrifuged for 15 h at 4°C and 74 400 x g (Beckman SW 32.1 Ti). From the gradient ...
-
bioRxiv - Microbiology 2020Quote: ... samples absorbance at 485 nm was measured in a 1 cm quartz cuvette using a DUTM 730 spectrophotometer (Beckman Coulter, Brea, CA, USA). 155 ...
-
bioRxiv - Cancer Biology 2022Quote: ... cells were incubated with an additional 400 µl 1× Binding Buffer and analyzed within 20 min by CytoFLEX S (Beckman Coulter Life Science). At least 1 × 104 cells were analyzed to determine the percentage of apoptotic cells ...
-
bioRxiv - Developmental Biology 2019Quote: ... washed, and fixed with 1% PFA (EM Sciences, Ft. Washington, PA) before analysis on a CyAn ADP Flow Cytometer (Beckman Coulter, Miami, FL). Data were collected on using a four-decade log amplifier and were stored in list mode for subsequent analysis using FlowJo Software.
-
bioRxiv - Immunology 2019Quote: 100 ul of cell suspension for each of the dilutions including undiluted (1:0) was mixed with 400 ul of 1X PBS in the ViCell cuvette (Beckman Coulter, Part # 723908). Cell counting was performed based on manufacturer’s instructions (Beckman Coulter Vi-cell XR – 490992 ...
-
bioRxiv - Pharmacology and Toxicology 2019Quote: ... for 10 m at 4°C and pelleted the lysates at 50,000×g for 1 h at 4°C using an Optima MAX-XP ultracentrifuge (Beckman Coulter, Brea, CA, USA). We resuspended cell fractions in 500 μL water and stored the samples at 4°C until analysis ...
-
bioRxiv - Microbiology 2019Quote: ... the extracts were added on top of layers in LCM buffer with 45% and 5% glycerol and ultracentrifuged at 77 000 x g for 1 h at +4°C (Beckman Coulter SW41Ti rotor). Viral nucleocapsids were recovered from the bottom of the ultracentrifuge tube ...
-
bioRxiv - Biochemistry 2022Quote: ... The polymerized actin samples were pelleted down by ultracentrifugation at room temperature (45000 rpm for 1 hour, TLA-55 rotor, Beckman Coulter, Indianapolis, IN). The pellets were rinsed once with a 1 mL G-buffer and re-suspended into ice-cold 0.5 mL G-buffer ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... Sequencing libraries for each replicate were generated via qPCR and sequencing libraries were purified and size selected using Ampure XP Beads at a 1.8:1 bead volume: library volume (Beckman Coulter, Brea, CA, USA). Library quality and transposition efficiency was accessed using a Fragment Analyzer and PROSize 2.0 (Agilent) ...
-
bioRxiv - Microbiology 2023Quote: ... The cells were then incubated for 30 minutes with the following antibodies: anti-HIV-1 core antigen (p24)-RD1 (Beckman Coulter catalog # 6604667), anti-TNFα PE/Dazzle 594 (BioLegend catalog # 502946) ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... the cell lysate was layered on top of a discontinuous OptiPrep® gradient (50 %, 35 %, 30 %, 20 %, and 15 %) and centrifuged at 135,000 g for 1 h at 4°C (Beckman L8-80M Ultracentrifuge, USA). The melanin pellet was recovered ...
-
bioRxiv - Cell Biology 2023Quote: ... 2.5 M sucrose in HK was added to the reaction to a final concentration of 1 M (“Input” sample) and transferred to 7 × 20 mm polycarbonate centrifuge tubes (Beckman Coulter cat #343775). HK buffer with 0.75 M sucrose was gently laid on top of the reaction mixture followed by a layer of HK ...
-
bioRxiv - Molecular Biology 2021Quote: The VHH-coding regions within parent libraries and 1-round target-enriched sublibraries were PCR amplified and purified using AMPure XP beads (Beckman Coulter, High Wycombe, UK). Then ...
-
bioRxiv - Microbiology 2022Quote: ... Reaction products were verified on a 1% agarose gel and purified using the AMPure XP magnetic Bead protocol (Beckman Coulter Inc., Brea, CA, USA). PCR2 was performed in a 25 μl reaction ...
-
bioRxiv - Systems Biology 2020Quote: ... and the resultant supernatant was ultracentrifuged at 100,000xg for 40 minutes at 4°C (rotor TLA 120.2; Beckman Coulter 343778 1 ml uncapped tubes).
-
bioRxiv - Physiology 2021Quote: ... sEV were further isolated from lEV-depleted supernatants following a 100,000 xg ultracentrifugation step for 1 hour at 4°C (rotor MLA-50, Beckman Coulter Optima MAX-XP Ultracentrifuge) and two washes in NaCl before resuspension in NaCl.
-
bioRxiv - Biochemistry 2024Quote: ... Triton X-100 was added to a 0.1% (v/v) and centrifuged for 45 minutes at 20,000 g at 1℃ (Beckman Coulter J2-21, JA 20 rotor). The supernatant was loaded onto a 5 ml HisTrap FF Crude column (Cytiva) ...
-
bioRxiv - Bioengineering 2023Quote: ... and a humidity of 85 % in 48-well Flowerplates containing 800 µL defined CGXII medium with 0.5 % (w/v) glucose inoculated to an OD600 of 1 by using the BioLector microbioreactor for 24 h (Beckman Coulter Life Sciences, Aachen, Germany). Increasing concentrations of ANT (final concentration of 0 ...
-
bioRxiv - Neuroscience 2023Quote: ... PI (1 μl/100 μl) was added 5 minutes before starting the reading on the flow cytometer CytoFlex S (Beckman Coulter, Brea, CA, USA). Results were shown and analyzed through CytExpert flow cytometry analysis software.
-
bioRxiv - Biophysics 2023Quote: ... Cell debris was removed by centrifugation at 20,000 rpm at 4°C for 1 h (Avanti® J-E centrifuge from Beckman Coulter, rotor: JLA 25.50). The supernatant was collected and loaded onto a pre-equilibrated 5 ml His-Trap FF crude column ...
-
bioRxiv - Cancer Biology 2023Quote: ... cells were washed with 1 ml flow buffer once then resuspended in 200 μl flow buffer and measured using a CytoFLEX cytometer (Beckman Coulter, Jersey City, NJ).
-
bioRxiv - Neuroscience 2023Quote: ... The samples were spun for 1 hour at RT in a Beckman ultracentrifuge using SW 120 Ti rotor (Beckman Coulter, Inc., Brea, CA, USA) at 100,000xg ...