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Citations for Beckman :
51 - 100 of 139 citations for Strat M Membrane Transdermal Diffusion Test Model since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... Membrane fractions were collected by centrifugation with a TLA55 rotor (Beckman) at 42,000 rpm for 20 min ...
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bioRxiv - Biochemistry 2020Quote: ... Then the ribosomes were pelleted through a sucrose cushion (1.1 M sucrose in HEPES:Polymix buffer with 15 mM Mg2+) at 50,000 rpm for two hours (TLS-55, Beckman), the pellet was dissolved in HEPES:Polymix buffer (5 mM Mg(OAc)2) ...
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bioRxiv - Cell Biology 2020Quote: ... containing the phosphatases and proteases inhibitors and centrifuged at 100,000xg for 1 h through a 3 ml 1.4 M sucrose cushion using a TL100 centrifuge (Beckman). The crude heavy nuclear fraction and purified nuclei were resuspended in buffer A and extracted with 1% Triton X-100 for 5 min at 4°C (cNM ...
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bioRxiv - Biochemistry 2023Quote: ... A discontinuous sucrose gradient was formed by overlaying with 6 ml of 35% sucrose and 3.5 ml of 5% sucrose in the same buffer containing 0.25 M Na2CO3 and centrifuged at 38,000 rpm for 18 h in an SW41 rotor (Beckman). Twelve fractions were collected from top to bottom ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and the solution layered over 6.0 ml of 0.25 M sucrose in a 13.2 ml centrifuge tube (Beckman, 344059). After centrifugation at 100,000 g for 90 min ...
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bioRxiv - Biochemistry 2020Quote: ... tubes and membranes were pelleted by ultracentrifugation (Optima XE-90, Beckman, DE) at 186,000 RCF ...
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bioRxiv - Biochemistry 2020Quote: ... tubes and membranes were pelleted by ultracentrifugation (Optima XE-90, Beckman, DE) at 186,000 RCF ...
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bioRxiv - Biochemistry 2020Quote: ... tubes and membranes were pelleted by ultracentrifugation (Optima XE-90, Beckman, DE) at 186,000 RCF ...
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bioRxiv - Physiology 2021Quote: ... Membranes were collected by ultracentrifugation in a 50.2 Ti rotor (Beckman Instruments) at 45 000 rpm for 1 h ...
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bioRxiv - Neuroscience 2021Quote: ... layered on the top of a discontinuous sucrose density gradient (0.32/1.0/1.2 M) and subjected to ultracentrifugation at 86,000 rpm (Beckman SW-60ti rotor) for 2 h at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... myelin was layered onto 3 mL ice cold 0.85 M sucrose solution in a 10 mL ultracentrifuge tube (Beckman) before centrifugation at 75 000 x g for 30 min at 4 °C on a Beckman Ultracentrifuge with MLA-55 rotor (acceleration 7 ...
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bioRxiv - Molecular Biology 2024Quote: ... and 1 M sucrose) at 100,000 rpm (543,000 × g) and 4°C for 1 h with a TLA110 rotor (Beckman Coulter) and an Optima MAX-TL Ultracentrifuge (Beckman Coulter) ...
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bioRxiv - Biophysics 2023Quote: ... and 1.3 M sucrose and 0.5 mL of 1.8 M sucrose at the bottom) in ultracentrifuge tubes (14 × 89 mm, Beckman Coulter). The sucrose gradients contained 0.03% (w/v ...
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bioRxiv - Microbiology 2024Quote: ... the solution was neutralised by the dropwise addition of 1 M hydrochloric acid centrifuged at 34,000 g (JA-20 rotor, Beckman) at 20 °C for 30 minutes to yield P5 and S5 ...
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bioRxiv - Biochemistry 2023Quote: ... Solubilized membranes were ultracentrifuged at 181,078 × g for 70 min (Beckman L-90K) and the supernatant containing Ycf1p was filtered through a 0.45 μm syringe filter (Pall ...
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bioRxiv - Plant Biology 2021Quote: ... The resuspended polysome samples were then loaded on 15% to 60% sucrose gradients and centrifuged at 50 000 RPM in a SW55.1 rotor and L8-M ultracentrifuge (Beckman Coulter). The gradient samples were fractionated using an ISCO absorbance detector (model # UA-5 ...
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bioRxiv - Biochemistry 2022Quote: ... To that was dispensed TMPRSS2 (reconstituted in 50% glycerol at 8.75 μM, 50x) in assay buffer (50 mM Tris pH 8, 150 mM NaCl, 0.01% Tween20) using a BioRAPTR (Beckman Coulter) to give a total reaction volume of 5 μL ...
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bioRxiv - Biochemistry 2020Quote: ... After increasing the NH4Cl concentration of the supernatant to 1 M to dissociate bS1 from 70S ribosomes the sample was centrifuged in a Type 70 Ti rotor (Beckman) at 60,000 g for 2 hours ...
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bioRxiv - Cell Biology 2022Quote: ... 1 M sucrose) and centrifuged at 100,000 RPM for 60 minutes at 4°C in a TLA100.3 rotor (Beckman Coulter, 349490) with 3.5 mL polycarbonate tubes (Beckman Coulter ...
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bioRxiv - Developmental Biology 2022Quote: ... the whole embryos were individually mounted on glass slides with spacers of approximately 100–500 µm, which were adjusted depending on the sample using sticky tape (CT-18S, Nichiban) and silicone vacuum grease (335148, Beckman). To view different sides of a single embryo ...
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bioRxiv - Cell Biology 2022Quote: ... and the post-nuclear supernatant was loaded onto an 8-step discontinuous sucrose density gradient (HEPES-buffered 0.2–2 M sucrose) and centrifuged at 55,000 rpm for 2h at 4 °C using an MLS50 rotor (Beckman Coulter). Extracellular acidification rate (ECAR ...
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bioRxiv - Cell Biology 2023Quote: ... loaded onto a discontinuous 0.75/1.0/2.33 M sucrose gradient and spin at 242,000 g for 1 h at 4 °C in a SW32Ti rotor (Beckman & Coutler). Growth cone fractions were collected at the interface load/0.75 M sucrose and non-growth cone fractions collected between 0.75/1.0 M sucrose and then analyzed by Western Blot.
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bioRxiv - Biophysics 2023Quote: ... followed by layering the cleared lysate onto 40 mL of 2 M sucrose cushion and ultracentrifugation in Ti-45 rotor (Beckman) at 42,000 rpm for 2 hours ...
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bioRxiv - Biochemistry 2022Quote: ... The membranes were pelleted at 75,000 x g using an MLA80 rotor (Beckman Coulter) for 1 h ...
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bioRxiv - Immunology 2020Quote: ... and antibodies against membrane markers were added (CD45, CD14, HLA-DR from Beckman Coulter). Intracellular staining was performed using the IntraPrep fixation permeabilization kit (Beckman Coulter ...
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bioRxiv - Biochemistry 2022Quote: ... membranes were pelleted by ultracentrifugation for 1.5 h (125,000 g, Beckman Type 45 Ti). The membrane pellet was resuspended in ∼10 pellet volumes of lysis buffer supplemented with 5 μM cotransin ...
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bioRxiv - Microbiology 2023Quote: ... Cytosolic and membranes components were separated by ultracentrifugation at 200,000 xg (Rotor TLA120 Beckman) for 45 min at 4°C ...
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bioRxiv - Biophysics 2024Quote: ... Total membranes were collected by centrifugation at 40,000 rpm (Ti-45 rotor, Beckman Coulter) for 1 hour at 4°C ...
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bioRxiv - Plant Biology 2021Quote: ... the samples were centrifuged at 35 000 RPM for 18 hours at 4°C in a 70Ti rotor and L8-M ultracentrifuge (Beckman Coulter). The ribosome pellets were then gently washed with RNase-free water and resuspended in 300 μl of resuspension buffer (100 mM Tris·HCl (pH 8.0) ...
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bioRxiv - Neuroscience 2020Quote: ... Each pool of samples was then purified using 79 μL beads buffer (20% PEG-8000 in 2.5 M NaCL, final concentrations) and 50 μL Ampure XP Beads (Beckman Coulter, A63881), and eluted in 7 μL NF H2O.
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bioRxiv - Cell Biology 2023Quote: ... ice-cold 5 mL 2.5 M sucrose was added to the bottom of an open-top plastic tube (Beckman-Coulter 344058), carefully topped up with ice-cold 5 mL 1.875 M sucrose ...
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bioRxiv - Neuroscience 2023Quote: ... Supernatant were loaded on top of a discontinuous 0.75/1.0/2.33 M sucrose density gradient and centrifuge at 242,000 x g for 60 min at 4°C (Beckman SWTi41 swing rotor). The growth cone depleted fraction was collected between 0.75 M and 1.0 M sucrose ...
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bioRxiv - Biochemistry 2024Quote: ... 1 mL reaction buffer containing substrates (25 μM-0.16 mM NADH and 2 mM pyruvate) was added to the cuvette of a spectrophotometer (DU® 700, Beckman Coulter). The reaction was started by adding cell lysate ...
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bioRxiv - Biochemistry 2024Quote: ... Non-solubilized materials were removed by centrifuging the sample at 41,000 r.p.m for 1 h at 4 °C using rotor type 45 Ti in the OptimaTM L-100 XP Ultracentrifuge (BECKMAN COULTER). The resulting supernatant containing the solubilized LysP-GFP was incubated with Ni2+-NTA resin (Qiagen ...
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bioRxiv - Plant Biology 2021Quote: ... cesium chloride continuous density gradient in resuspension buffer and centrifuged at 34,000 m for 5 hours at 20 °C using an SW41 Ti rotor (Beckman Coulter, Brea, CA). The virus particles were observed in the lower of two pale white bands in the gradient ...
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bioRxiv - Biochemistry 2020Quote: ... pH 7.4 containing 0.25 M sucrose and centrifuged at 53,000 rpm (150,000 g) in an Optima TLX ultracentrifuge (Beckman Coulter Inc., Brea, CA, USA) with a TLA100.3 rotor for 90 min at 4 °C ...
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bioRxiv - Plant Biology 2020Quote: ... Soil pH and EC were 8.3 and 12 dS m−1 that were measured by a pH meter (Beckman 45 Modal, US) and EC meter (VWR Conductivity Meter DIG2052 ...
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bioRxiv - Immunology 2021Quote: ... was loaded onto the top of 6–8 M GuHCl gradients and centrifuged in a Beckman Optima L-90K Ultracentrifuge (Beckman SW40 rotor) at 40,000 rpm for 2.75 h 15°C ...
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bioRxiv - Bioengineering 2022Quote: ... The supernatant was transferred into polycarbonate tubes and centrifuged at 20,000 ×g for 45 min at 4°C to pellet down m/lEVs using an Optima XE-90 ultracentrifuge equipped with a 50.2 Ti rotor (Beckman Coulter, Indianapolis, IN). Next ...
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bioRxiv - Neuroscience 2022Quote: ... was loaded onto a sucrose gradient made up of 5 ml 1.2 M sucrose on the bottom and 5 ml of 0.8 M sucrose at the top in thin wall ultracentrifugation tubes (Beckman Coulter, Cat # 344059). The loaded gradient was ultracentrifuged at 69,150 x g for 1 h at 4 °C (SW 41 Ti Swinging-Bucket Rotor ...
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bioRxiv - Synthetic Biology 2024Quote: ... 200 μL of supernatant was loaded onto sucrose gradients (10–50% sucrose in 0.6 M KP buffer) prepared in tubes (14 × 89 mm, Open-Top Thinwall Ultraclear tubes [Beckman Coulter, CA, USA]) using a Gradient Master (Beckman Coulter) ...
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bioRxiv - Cell Biology 2023Quote: ... Supernatants were loaded onto a 1 M sucrose cushion and then pelleted by ultracentrifugation by using a TLA100.3 rotor (Beckman Coulter, cat#349490) for 1 hour at 100,000 rpm at 4°C ...
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bioRxiv - Bioengineering 2024Quote: ... The supernatant was then transferred into polycarbonate tubes and centrifuged at 20,000 ×g for 45 min at 4°C to pellet down m/lEVs using an Optima XE-90 ultracentrifuge fitted with a 50.2 Ti rotor (Beckman Coulter, Indianapolis, IN). Following that ...
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bioRxiv - Developmental Biology 2024Quote: Cleared S10 fractions were adjusted to 500 μL with Subunit Lysis Buffer and overlaid onto 2 mL of a 1 M sucrose cushion prepared in Subunit Buffer in a 15×51 mm 3 mL thickwall polycarbonate ultracentrifuge tube (Beckman Coulter, 349622) on ice ...
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bioRxiv - Molecular Biology 2022Quote: ... Membranes were harvested by ultracentrifugation at 240,000 g in a 50.2 Ti rotor (Beckman Coulter) at 4 °C for 2 h ...
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bioRxiv - Biochemistry 2022Quote: ... Membrane vesicles were removed by centrifugation at 285,000 x g 1 hr (Beckman Optima L100XP). The clarified lysate was then fractionated by ammonium sulfate precipitation with fractions pelleted at 10,000 x g for 10 min and the 40-70% saturating fraction was retained and exchanged via centrifugal concentrator (Amicon 30 kDA molecular weight cutoff ...
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bioRxiv - Biophysics 2020Quote: ... DDM solubilized membranes were cleared by centrifugation at 45,000 rpm in 50.2Ti rotor (Beckman Coulter) for 45 min and the supernatant was purified using affinity chromatography with a Ni-NTA column (Qiagen) ...
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bioRxiv - Microbiology 2021Quote: ... The soluble fraction was separated from membranes and cell debris by ultracentrifugation (Beckman, Ti 45) at 22,000 rpm for 40 minutes ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were isolated by ultracentrifugation for 45 min at 42,000 rpm (45 Ti rotor, Beckman), 4°C ...
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bioRxiv - Microbiology 2021Quote: ... resuspended in 150 μl 0.1 M HEPES/NAOH pH 7.0 and purified for 48 h (250,000 × g, 4 °C; Beckman Optima LE-80K, rotor SW60-Ti) using a CsCl gradient (0.45 g/ml ...