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Citations for Beckman :
51 - 100 of 172 citations for L Asparagine H2O 13C4; D3; 15N2 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... ultracentrifugation was performed at 100,000 g for 1 h (Optima L-90K ultracentrifuge; Beckman Coulter). The supernatant was stored at -20°C ...
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bioRxiv - Molecular Biology 2023Quote: ... 4 °C using the swing-out rotor SW32 on an Optima L-90K (Beckman Coulter) ultracentrifuge ...
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bioRxiv - Developmental Biology 2024Quote: ... Ultracentrifugation was carried out with a SW55Ti rotor and Optima L-90 Ultracentrifuge (Beckman Coulter). Ribosome profiles were recorded by ISCO UV absorbance reader ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were centrifuged in an SW.41 rotor (OptimaTM L-100 XP, Beckman Coulter, Brea, CA) at 39,000 rpm for 16 h at 4°C ...
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bioRxiv - Bioengineering 2021Quote: ... The supernatant was then ultracentrifuged for 2 hours using Optima™ L-80XP Ultracentrifuge (Beckman Coulter) at 100,000 x g at 4°C ...
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bioRxiv - Bioengineering 2021Quote: ... The supernatants were then centrifuged at 100,000 × g for 90 min (Beckman Coulter, Optima L-100XP) to obtain the exosomes ...
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bioRxiv - Cell Biology 2020Quote: ... and centrifuged for 90 min at 4 °C in an Optima L-90K ultracentrifuge (Beckman Coulter) at 28 000 rpm (~97 000 g ...
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bioRxiv - Microbiology 2021Quote: ... Viral stock was thawed and concentrated by ultracentrifugation (Beckman Coulter Optima L-100k; SW32 Ti rotor) at 25,000 x g for 2 h at 4°C on a 20% sucrose cushion (Sigma-Aldrich ...
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bioRxiv - Immunology 2021Quote: Blood cell counts were determined from EDTA blood using an HMX A/L analyzer (Beckman Coulter).
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bioRxiv - Immunology 2020Quote: ... haemoglobin and haematocrit were determined from EDTA blood using a HMX A/L analyzer (Beckman Coulter).
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bioRxiv - Pathology 2021Quote: ... the stock was thawed and concentrated by ultracentrifugation (Beckman Coulter Optima L-100k; SW32 Ti rotor) at 25,000 x g for 2 hours at 4 °C on a 20% sucrose cushion (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2024Quote: Bacteria were collected in 1 L buckets by centrifugation at 5000 rpm (JLA8.1 rotor, Beckman-Coulter) for 20 min ...
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bioRxiv - Biochemistry 2024Quote: ... Bacteria were collected in 1-L buckets by centrifugation at 5000 rpm (JLA8.1 rotor, Beckman-Coulter) for 20 minutes ...
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bioRxiv - Biophysics 2023Quote: ... The lysate was centrifuged at 25.000 x g (rotor 45 Ti, Optima L-90K Ultracentrifuge, Beckman Coulter International S.A. ...
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bioRxiv - Biochemistry 2022Quote: ... Insoluble material was removed by ultracentrifugation at 158,000 ×g for 1h (Beckman L-90K, Ti70 rotor), and membranes were filtered with a 0.45 μm syringe filter and applied to a 0.5 mL anti-FLAG M2 affinity gel in a column (Millipore Sigma ...
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bioRxiv - Cancer Biology 2023Quote: ... and ultracentrifuged at 100,000g for 90 min at 4°C (Optima L-90K Ultracentrifuge, Beckman Coulter). Pelleted EVs were resuspended in 200 μl PBS ...
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bioRxiv - Microbiology 2024Quote: ... after which virus was collected by ultracentrifugation using an Optima L-80 XP ultracentrifuge (Beckman Coulter) at 100,000 x g in a Sw 41 Ti rotor ...
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bioRxiv - Immunology 2023Quote: ... hemoglobin and hematocrit were determined from EDTA-treated blood using an HMX A/L analyzer (Beckman Coulter).
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bioRxiv - Cell Biology 2024Quote: ... 086 x g for 2.5 hours at 4°C using Optima L-100 XP ultracentrifuge (Beckman Coulter), and the pellet was resuspended in LHCN medium ...
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A Drug-Free Pathogen Capture and Neutralizing Nasal Spray to Prevent Emerging Respiratory InfectionsbioRxiv - Bioengineering 2023Quote: ... The viral stock was thawed and concentrated by ultracentrifugation (Beckman Coulter Optima L-100k; SW32 Ti rotor) on a 20% sucrose cushion (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2024Quote: ... before pelleting cell debris by ultracentrifugation at 40,000 rpm (Beckman Coulter Optima L-90K, 70 Ti rotor) at 4 °C for 30 min.
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bioRxiv - Microbiology 2024Quote: ... The filtrate was ultracentrifuged at 200,000 xg for 2 hr (Optima L-100 XP ultracentrifuge; Beckman Coulter). Supernatants were discarded ...
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bioRxiv - Biophysics 2024Quote: ... Cells were pelleted from 2 L cultures by centrifugation at 4,785g (5,000 rpm in Beckman JLA-8.100) for 20 min ...
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bioRxiv - Molecular Biology 2024Quote: ... in an ultracentrifuge Optima L-100 XP with a fixed angle rotor Type 70Ti (Beckman Coulter, USA) at 50,000 rpm (183,960 × g ...
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bioRxiv - Cell Biology 2021Quote: ... 4 °C for 2 h in an Optima L-100XP ultracentrifuge (with SW 55 Ti rotor, Beckman Coulter). Fractionation was done manually by collecting every 200 μl volume from the top till the bottom of each centrifuge tube ...
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bioRxiv - Microbiology 2023Quote: ... The filtrate was subjected to ultracentrifugation at 200,000xg for 2 h (Optima L-100 XP ultracentrifuge; Beckman Coulter). Supernatants were discarded ...
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bioRxiv - Microbiology 2024Quote: ... (The filtrate was subjected to ultracentrifugation at 100,000g for 2 h (Optima L-90 K ultracentrifuge; Beckman Coulter). The supernatant was discarded and the pellet was washed with sterile PBS and filtered through a sterile 0.2 μm-pore polyvinylidene difluoride (PVDF ...
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bioRxiv - Microbiology 2024Quote: ... The eluent was ultracentrifuged (100,000 ×g, 4°C, 16 h) using Optima L-100 XP Ultracentrifuge (Beckman Coulter) to sediment ribosomes.
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bioRxiv - Cell Biology 2022Quote: ... Lentiviral particles were then concentrated by centrifugation for 2 hours at 20,000 rcf (Beckman Coulter, Optima L-100K) in round bottom polypropylene tubes (Beckman Coulter ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were centrifuged for 20 h at 190,000 x g in a SW-40 Rotor (Beckman Optima L-70 or Beckman Optima L-80 XP ...
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bioRxiv - Molecular Biology 2020Quote: ... followed by ultracentrifugation at 200000 g for 120 minutes (Type 70 Ti rotor, Optima L-80 XP, Beckman Coulter). The pellet was washed in PBS and collected by ultracentrifugation at 200000 g for 120 minutes [17] ...
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bioRxiv - Microbiology 2020Quote: ... supernatants were harvested and filtered through a 0.45μm filter and centrifuged at 27,000 rpm for 3h at 4°C with a SW41 rotor and Optima L-90 centrifuge (Beckman). Pellets were resuspended in PBS prior to use for western blot analysis.
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bioRxiv - Microbiology 2021Quote: ... and 500 μl of a 10 ml resuspension of cells from each flask diluted with 19.5 ml Isoton II Diluent (Beckman). PD time ...
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bioRxiv - Microbiology 2020Quote: ... density gradient centrifugation was performed in 5,1 ml quick-seal tubes in a NVT 65.2 rotor (near vertical) using Optima L-90K centrifuge (Beckman coulter). CsCl medium having an average density of 1,72g/ml was loaded with 6μg of total extracted DNA ...
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bioRxiv - Microbiology 2023Quote: ... The filtrate was subjected to ultracentrifugation at 200,000 xg for 2 h (Optima L-100 XP ultracentrifuge; Beckman Coulter). Supernatants were discarded ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were then centrifuged at 82,600×g at 4°C for 2 h (Beckman ultracentrifuge optima L-100 XP). After the first ultracentrifugation ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples were sedimented onto poly-L-lysine coated coverslips at 12,000 rpm (22,579 x g) in an Avanti J-25 centrifuge (Beckman Coulter) for 1 hr and post-fixed with methanol for 5 min at −20 °C ...
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bioRxiv - Neuroscience 2024Quote: ... and PBS was added to reach a 4.5 mL tube filling and centrifuged at 120,000xg for 100 min at 4°C in the L-60 Optima Ultracentrifuge (Beckman Coulter ...
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bioRxiv - Cell Biology 2024Quote: ... The supernatant was then subjected to ultracentrifugation at 100,000 x g for 2 h at 4 °C to sediment small EVs (Optima L-80K ultracentrifuge, Beckman Coulter ...
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bioRxiv - Pathology 2023Quote: ... and culture supernatant was concentrated by ultracentrifugation (2h at 26,000rpm; Ultraclear Tubes; SW28 rotor and Optima L-100 XP Ultracentrifuge; Beckman). Viruses were suspended in cold sterile PBS and titrated by transduction of Jurkat cells for 48h ...
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bioRxiv - Immunology 2023Quote: ... and then centrifuged at 260,800 x g for 2.5 h at 4°C in an Optima L-100XP ultracentrifuge (Beckman–Coulter) using the SW41Ti rotor with brake ...
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bioRxiv - Synthetic Biology 2024Quote: ... it was placed in a SW28.1 rotor and centrifuged in a Beckman L-90K ultracentrifuge (Beckman Coulter, Pasadena, U.S.A) at 125,000 g for 20 hours at 4 °C ...
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bioRxiv - Molecular Biology 2024Quote: ... were generated by transient calcium phosphate transfection of HEK-293T cells and concentrated from culture supernatant by ultracentrifugation (2 hours at 128,000xg; Ultraclear Tubes; SW28 rotor and Optima L-100 XP Ultracentrifuge; Beckman). The viruses were suspended in cold sterile PBS and titrated by transduction of Jurkat cells for 48 hours ...
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Enhanced kinase translocation reporters for simultaneous real-time measurement of PKA, ERK, and Ca2+bioRxiv - Bioengineering 2024Quote: ... Lentivirus particles were pelleted from these samples by ultracentrifugation at 100,000x g for 120 minutes (Optima L-90K, using a SW-32 Ti rotor, Beckman), and the pellets were resuspended in either 1mL of complete medium or neuron culture media.
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bioRxiv - Biochemistry 2024Quote: ... and membranes were removed by ultracentrifugation at 396,000 ×g for 1.5 h (55,000 rpm, Beckman L-90K, Ti70 rotor). The supernatant was filtered with a 0.45 μm syringe filter and applied to 1.5 mL anti-FLAG M2 affinity gel (Millipore Sigma ...
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bioRxiv - Molecular Biology 2020Quote: ... Gradients were centrifuged at 39 000 rpm for 2.5 h at 4°C in an Optima L-100XP ultracentrifuge (Beckman– Coulter) using the SW41Ti rotor with brake ...
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bioRxiv - Cell Biology 2021Quote: ... The post-nuclear supernatants were ultracentrifuged using SW41Ti rotor at 100,000 g for 3 h at 4 °C on Optima L-90K (Beckman Coulter). The fraction between 0.4–0.5 M sucrose was collected ...
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bioRxiv - Developmental Biology 2020Quote: ... Virus-containing supernatant was collected for two consecutive days and concentrated by ultracentrifugation for 2 hours at 19,400 rpm using an Optima L-80 XP Ultracentrifuge (Beckman Coulter).
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bioRxiv - Microbiology 2022Quote: ... was added before ultracentrifugation at 20,000 × g for 2 hours at 10°C in an Optima L-90K Ultracentrifuge with the SW32Ti rotor (Beckman Coulter). Supernatants were carefully discarded by aspiration and the 6 phage pellets were resuspended with 0.1 ml of TM buffer (50 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2021Quote: ... and HDL (1.063-1.021g/L) were isolated by sequential DGUC using an Optima XPN-80 Ultracentrifuge with SW32Ti or SW41Ti rotors (Beckman-Coulter). Lipoproteins were dialyzed in 1X PBS (4L ...