Labshake search
Citations for Beckman :
51 - 100 of 162 citations for Hepatitis E Virus Capsid Protein ORF2 Mouse Fc Tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2020Quote: ... The percentage of GFP-positive cells was measured on a CXP cytometer (Cytomics FC 500, Beckman Coulter) using CXP Software version 1.0 according to the manufacturer’s instructions.
-
bioRxiv - Immunology 2022Quote: ... FCS files generated by the BD FACS DeVa software (Bd Bio Sciences) or CytExpert software (Beckman Coulter) were analyzed using FlowJo software V10 ...
-
bioRxiv - Immunology 2020Quote: ... normalization and FCS file generation the files were further analyzed in Cytobank (Beckman Coulter, Brea, CA, USA). Analysis of the cells was carried out on live singlets within the pan-hemocyte marker ...
-
bioRxiv - Systems Biology 2021Quote: HeLa Kyoto and R1/E mESCs (gifts from Hyman lab, Dresden) and HEK293 (gift from Beckman lab, Berlin), Ptk2 cell lines (gift from Simons lab ...
-
bioRxiv - Microbiology 2021Quote: 1 mL pseudoparticles were pelleted using a TLA-55 rotor with an Optima-MAX-E ultracentrifuge (Beckman Coulter) for 2 hours at 42,000 rpm at 4°C ...
-
bioRxiv - Molecular Biology 2022Quote: ... followed by centrifugation at 15,500 x g for 25 min in a JA 25.50 rotor in an Avanti J-E centrifuge (Beckman). The supernatant was transferred to a 50-mL conical tube ...
-
bioRxiv - Molecular Biology 2022Quote: ... Bacteria were harvested at 4,000 x g for 25 min in a JLA-8.1000 rotor in an Avanti J-E centrifuge (Beckman), and the pellet was transferred to a 50-mL conical tube (Sarstedt ...
-
bioRxiv - Microbiology 2022Quote: ... Supernatant was collected and virus pelleted through 30% (w/v) sucrose cushion at 151,000 × g (using a Beckman SW 32 Ti rotor) for 3.5 hours at 10°C ...
-
bioRxiv - Immunology 2022Quote: ... Individual and cleaned FCS files were exported for analysis and uploaded to the Cytobank software platform (Beckman Coulter) for subsequent analysis with CITRUS (cluster identification ...
-
bioRxiv - Microbiology 2023Quote: Gradient fractions of fluorescent LEVs and SEVs were analysed by FC on a Gallios Flow Cytometer (Beckman Coulter) using an optimised procedure ...
-
bioRxiv - Biochemistry 2021Quote: ... after which the lysate was clarified by centrifugation at 30,000 x g for 60 min at 4 °C in a JA 25.50 rotor in an Avanti J-E centrifuge (Beckman). Nucleic acids in the clarified lysate were precipitated by slowly adding polyethyleneimine with constant stirring in an ice bath to a final concentration of 0.4% ...
-
bioRxiv - Biochemistry 2021Quote: ... and the lysate was clarified by centrifugation at 30,000 x g for 60 min at 4 °C in a JA25.50 rotor in an Avanti J-E centrifuge (Beckman). The clarified lysate was then added to 10 mL of Ni-NTA Agarose beads (Invitrogen ...
-
bioRxiv - Microbiology 2023Quote: ... All centrifugation steps were performed at 4 °C in an Avanti J-E high-speed fixed-angle centrifuge (Beckman Coulter Life Sciences ...
-
bioRxiv - Biophysics 2020Quote: ... lysate was centrifuged at 5 °C with 13000 rpm for 15 min by Avanti J-E Ultra Centrifuge (Beckman Coulter). Supernatant from centrifugation was filtered with 0.45 μm SFCA syringe filters (Sigma Aldrich ...
-
bioRxiv - Microbiology 2021Quote: 3 mL of pseudoparticles were pelleted using a TLA-55 rotor with an Optima-MAX-E ultracentrifuge (Beckman Coulter, Inc.) for 2 hours at 42,000 rpm at 4°C ...
-
bioRxiv - Biochemistry 2019Quote: ... The supernatant from 2,000 × g was transferred to 30mL conical tube and then centrifuged at 10,000 × g for 10 min at 4°C (# Avanti J-E JA25-50 Beckman Coulter). The supernatant from this spin was filtered through a 0.22-μm mesh filter (# SLGP033RS EMD Millipore ...
-
bioRxiv - Synthetic Biology 2019Quote: ... then centrifuged at 5000 x g and 10°C for 10 minutes (Avanti□ J-E Centrifuge, Beckman Coulter, Indianapolis, IN). After disposing the supernatant ...
-
bioRxiv - Biochemistry 2022Quote: ... A soluble fraction was obtained after centrifugation of the lysate at 19,000 rpm for 40 min at 4 °C performed using Avanti J-E with a rotor JA-20 (BECKMAN COULTER) to remove insoluble debris.
-
bioRxiv - Plant Biology 2024Quote: ... The mixture was then centrifuged for 20 min at 9,700 g at 6°C using an Avanti J-E centrifuge and a JLA-16.250 rotor (Beckman Coulter, USA). The supernatant was separated and centrifuged for 2.5 h at 200,000 g at 6°C using an Optima XPN-90 ultracentrifuge and a Type 45 Ti rotor (Beckman Coulter ...
-
bioRxiv - Plant Biology 2024Quote: ... The suspension was then centrifuged for 20 min at 9,700 g at 6°C using an Avanti J-E centrifuge and a JLA-16.250 rotor (Beckman Coulter, USA). The supernatant was separated and centrifuged for 3 h at 200,000 g at 6°C using an Optima XPN-90 ultracentrifuge with a Type 45 Ti rotor (Beckman Coulter ...
-
bioRxiv - Microbiology 2021Quote: ... Samples were diluted to a DNA concentration of 50 ng/µl followed by ligation of 454 sequencing adaptors and molecular identifier (MID) tags using the SPRIworks Fragment Library System II for Roche GS FLX+ DNA Sequencer (Beckman Coulter), excluding fragments smaller than 350 base pairs ...
-
bioRxiv - Immunology 2022Quote: ... Targeted amplification was performed for 13 cycles and the products were separated according to size into <400 bp (DNA barcode-tags) and >400 bp (the TCR sequences) using 0.6x SPRIselect beads (Beckman Coulter, B23318). Separate processing of the >400 bp bead-bound TCR sequences and the <400 bp in solution DNA barcodes was conducted according to manufacturer’s instruction and the TCR amplification products were sequenced on a NovaSeq running a 150 paired-end program ...
-
bioRxiv - Cancer Biology 2020Quote: ... samples were suspended in FACS buffer (PBS, 20 mM EDTA pH 8.0, 2% FCS) and acquired using a CyAn ADP9 flow cytometer (Beckman Coulter), FACS LSRII Fortessa or FACSymphony (both BD Biosciences) ...
-
bioRxiv - Developmental Biology 2021Quote: ... Cells were washed twice and resuspended in FC buffer containing 7AAD (LifeTechnologies, Musgrave, Australia) for acquisition on a Cytoflex flow cytometer (Beckman Coulter Life Sciences ...
-
bioRxiv - Immunology 2023Quote: ... Cells were washed twice and resuspended in FC buffer containing 7AAD (LifeTechnologies, Musgrave, Australia) for acquisition on either a Cytoflex (Beckman Coulter Life Sciences ...
-
bioRxiv - Microbiology 2022Quote: A 3 ml volume of pseudoparticles was pelleted using a TLA-55 rotor with an Optima-MAX-E ultracentrifuge (Beckman Coulter) for 2 hours at 42,000 rpm at 4°C ...
-
bioRxiv - Plant Biology 2019Quote: ... The corresponding TAG spots were scraped and quantified for radioactivity using a LS 6500 multi-purpose scintillation counter (Beckman-Coulter, Mississauga, Canada).
-
bioRxiv - Cancer Biology 2020Quote: ... the pellets were resuspended in 500 μl PBS and analyzed by flow cytometry using the FITC channel of a FC-500 flow cytometer (Beckman Coulter). All manipulations were performed in 1.7 ml Eppendorf tubes ...
-
bioRxiv - Cancer Biology 2021Quote: To evaluate the proportion of white blood cells from R26-GB2 mice expressing the GSDMB-HA-GFP transgenes we analyzed GFP emission by Flow Cytometry (Cytomics FC 500MPL, Beckman Coulter). Total leukocyte cells ...
-
bioRxiv - Cell Biology 2020Quote: FCS-EV depleted medium was first prepared by centrifuging DMEM with 20% FCS at 200Kg overnight with a 45Ti rotor (Beckman Coulter). Supernatant was then recovered by pipetting without taking the last 5mL to avoid disturbing the pellet ...
-
bioRxiv - Microbiology 2023Quote: ... Fluorescent LEVs and SEVs from the corresponding pellets were analysed by flow cytometry (FC) on a Gallios Flow Cytometer (Beckman Coulter) using an optimised procedure ...
-
bioRxiv - Microbiology 2020Quote: ... and the resultant lysate was transferred into SS34 tubes for further centrifugation at 18,000 rpm (JA-25.50 rotor, Beckman Coulter Avanti J-E) for 30 min ...
-
bioRxiv - Immunology 2020Quote: ... recombinant protein and CD14 antibody (Beckman) were used at 20 μg/ml ...
-
bioRxiv - Molecular Biology 2023Quote: Proteins were collected by centrifugation (Beckman-Coulter JA-14 rotor ...
-
bioRxiv - Cell Biology 2022Quote: ... pelleted and then re-suspended in ice-cold 0.2% FCS in DPBS for flow cytometry analysis using CytoFLEX S (Beckman Coulter, Bucks, UK). Cells were excited at 488 nm and the emission fluorescence was collected at 535 nm ...
-
bioRxiv - Cell Biology 2022Quote: ... The plate was incubated for 15 min in darkness and measured in the flow cytometer FC 500 (Beckman-Coulter, Brea, CA, USA). This experiment was repeated 3 times on different days.
-
bioRxiv - Immunology 2022Quote: ... and mRNA PCR products were separated from sample tag and AbSeq products with double-sided size selection using Agentcourt® AMPure® XP magnetic beads (Beckman Coulter, #A63880). mRNA and Sample Tag products were further amplified ...
-
bioRxiv - Cell Biology 2023Quote: ... IgG2a Mouse-PE (A09142, Beckman Coulter), IgG1κ Mouse-AF647 (565571 BD Biosciences) ...
-
bioRxiv - Biophysics 2023Quote: ... Cell debris was removed by centrifugation at 20,000 rpm at 4°C for 1 h (Avanti® J-E centrifuge from Beckman Coulter, rotor: JLA 25.50). The supernatant was collected and loaded onto a pre-equilibrated 5 ml His-Trap FF crude column ...
-
bioRxiv - Cell Biology 2020Quote: ... Protein aggregates were pelleted by ultracentrifugation of the proteins individually at 80,000 rpm in TLA 100 (Beckman Coulter) for 15 minutes ...
-
bioRxiv - Immunology 2021Quote: ... recombinant protein and anti-CD14 antibody (Beckman) were used at 20 μg/ml ...
-
bioRxiv - Physiology 2020Quote: ... one bird per pen in Experiment 2) and centrifuged for 5 min at 17013 × g using a Beckman microfuge (Model E 348720, Beckman Instruments, INC, Palo Alto, CA). Then the viscosity of ileal supernatant was measured using a Brookfield cone-plate digital viscometer (Model LVDV-Ⅲ ...
-
bioRxiv - Biophysics 2023Quote: ... Cells were harvested by centrifugation at 8000 g at 4°C for 20 min (Avanti® J-E centrifuge from Beckman Coulter, rotor: JLA 16.250).
-
bioRxiv - Biochemistry 2023Quote: ... Pellets were washed and gently resuspended in 1 ml of PBS and centrifuged at 50,000 × g for 1 h at 4°C (Beckman Optima MAX-E, TLA-55 rotor). The resulting pellets were resuspended in 500 μl of PBS to obtain concentrated lentiviral stocks that were stored at −80°C in aliquots ...
-
bioRxiv - Bioengineering 2023Quote: ... subtilis spores were collected from the culture by the centrifugation at 8000 rpm at 4 °C for 20 min (Avanti J-E, Beckman Coulter Life Science, IN, USA). Supernatant was removed and the spore pellets were resuspended in fresh 100 mM PBS pH 7.4 ...
-
bioRxiv - Biochemistry 2024Quote: ... Pellets were washed and gently resuspended in 1 mL of PBS and centrifuged at 50,000 x g for 1 h at 4°C (Beckman Optima MAX-E, TLA-55 rotor). Pellets were resuspended in 500 μl of PBS to obtain concentrated lentiviral stocks that were stored at −80°C in aliquots ...
-
bioRxiv - Systems Biology 2020Quote: ... tetramethylrhodamine (TRITC)-conjugated anti-mouse IgM (#731781, Beckman Coulter), and Alexa-594-conjugated anti-rabbit IgG (A11012 ...
-
bioRxiv - Immunology 2023Quote: ... for mouse samples or a CytoFLEX LX (Beckman Coulter) for human samples ...
-
bioRxiv - Microbiology 2020Quote: ... The proteins were sedimented in the SW40Ti rotor (Beckman Coulter) at 100,000 × g for 17 h at 4 °C ...
-
bioRxiv - Microbiology 2022Quote: Supernatant containing proteins and membrane were ultracentrifuged at 200,000xg (Beckman Coulter Ultracentrifuge ...