Labshake search
Citations for Beckman :
51 - 100 of 1239 citations for 7 bromo 1 methyl 1 3 dihydro 2H benzo d imidazol 2 one since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Developmental Biology 2020Quote: ... products of one plate were pooled into one tube and cleaned up using AMPure XP beads (Beckman Coulter). After in vitro transcription and fragmentation ...
-
bioRxiv - Developmental Biology 2024Quote: Cleared S10 fractions were adjusted to 500 μL with Subunit Lysis Buffer and overlaid onto 2 mL of a 1 M sucrose cushion prepared in Subunit Buffer in a 15×51 mm 3 mL thickwall polycarbonate ultracentrifuge tube (Beckman Coulter, 349622) on ice ...
-
bioRxiv - Genomics 2020Quote: ... The PCR products were purified by using 1.3X Agencourt AMPure XP beads (2 or 3 cycles; Beckman Coulter) and evaluated by using an Agilent High Sensitivity DNA Kit on an Agilent 2100 Bioanalyzer (Agilent) ...
-
bioRxiv - Biochemistry 2021Quote: ... Lentivirus from the cell supernatant was collected 24h later by ultracentrifugation with a Sw28 rotor (Beckman Coulter; 22,000 rpm, 2h, 4°C). Finally ...
-
bioRxiv - Cancer Biology 2024Quote: ... The plates were then centrifuged at 2000 rpm (931 x g) for 2h at 30°C (Allegra® X-15R Centrifuge (Beckman Coulter), rotor SX4750A) ...
-
bioRxiv - Microbiology 2021Quote: ... incubated samples for 7 min, centrifuged (650 x g, 7 min, RT) and measured them by flow cytometry (Cytomics FC500, Beckman Coulter, USA) after resuspension in Tyrode’s buffer ...
-
bioRxiv - Biochemistry 2024Quote: In 7 x 20mm Thickwall Polycarbonate tubes (Beckman Coulter), paclitaxel-stabilized MTs were mixed at room temperature with Kif5b proteins in BRB80 plus indicated the nucleotides or metal salts ...
-
bioRxiv - Microbiology 2020Quote: ... by centrifugation in a Beckman L7-65 ultracentrifuge at 25,000 rpm for 2h at 4°C using a Beckman SW28 rotor (Beckman Coulter, Brea, CA, USA). Supernatants were aspirated and the pellets were re-suspended in PBS (pH 7.4) ...
-
bioRxiv - Neuroscience 2022Quote: ... DNase-treated RNA was subsequently purified using RNase-free Agencourt AMPure XP beads (Beckman Coulter; 2:1 volume of beads:sample).
-
bioRxiv - Molecular Biology 2024Quote: ... 14 µl and 7 µl of SPRIselect Beads (Beckman Coulter) were used in the first and second steps ...
-
bioRxiv - Molecular Biology 2024Quote: ... 14 µl and 7 µl of SPRIselect Beads (Beckman Coulter) were used in the first and second steps ...
-
bioRxiv - Biochemistry 2020Quote: ... Protein expression was induced with 0.4 mM Isopropyl β-D-1-thiogalactopyranoside and cells were grown at 37 °C overnight before harvesting by centrifugation at 4000 rpm (Beckman Coulter Avanti JXN-26; JLA8.1 rotor) at 10 °C for 10 min.
-
bioRxiv - Cancer Biology 2020Quote: ... CD14 BV605 (1:100) and CD11b PC5 (1:100, Beckman Coulter). Cells were analyzed on the FACSCelesta (BD).
-
bioRxiv - Systems Biology 2022Quote: ... and purification with Ampure XP beads (1:1 ratio, Beckman Coulter) produced a purified cDNA library ...
-
bioRxiv - Genomics 2020Quote: ... cleaned up with 1:1 AMPureXP beads (Beckman Coulter, Brea, CA). ...
-
bioRxiv - Plant Biology 2020Quote: ... purified with the double AMPure XP cleanup (1:1) (Beckman Coulter), and checked with a High sensitivity DNA chip (Agilent technologies) ...
-
bioRxiv - Molecular Biology 2022Quote: Procyclic form cell lines for depletion of either TbPH1 or TbKifX2A were induced at a density of 2×106 cells/ml with 1 µg/ml doxycycline and growth monitored daily with a Z2 Coulter Counter Analyzer (Beckman Coulter). Cells were diluted back to the initial cell density every day ...
-
bioRxiv - Microbiology 2024Quote: Intracellular HIV-1 p24 staining was performed on fixed U1 and ACH-2 cells with KC57-FITC (Beckman Coulter, Cat#6604665). After allowing at least 30 minutes for fixation ...
-
bioRxiv - Microbiology 2024Quote: ... Four working concentrations were achieved through 1:2 serial dilutions using a Biomek i7 liquid handler (Beckman Coulter, ED. Fig. 1a). Precultures were grown overnight and diluted to an OD600 nm of 1 (WT ...
-
bioRxiv - Neuroscience 2022Quote: ... The material in the flow-through of the washing steps was pelleted by ultracentrifugation (at 100,000g for 2h; Optima MAX-XP Ultracentrifuge from Beckman Coulter equipped with a TLA55 rotor) before lysis as described (Théry et al. ...
-
bioRxiv - Cancer Biology 2022Quote: ... 10 nM leptomycin b, vehicle control or library compounds (Supplementary Tables 2, 3) were added using an Echo liquid handler (Labcyte/Beckman Coulter). Cells were incubated for a further 24 h then fixed in 4% paraformaldehyde and stained with DAPI and Alexa Fluor 488 Phalloidin (ThermoFisher) ...
-
bioRxiv - Genetics 2020Quote: ... Timepoints were measured every 2-3 days by flow cytometry analysis of >7,000 cells (either a BD Accuri C6 or Beckman Coulter CytoFLEX). Data was analyzed using Cytoflow (https://github.com/bpteague/cytoflow ...
-
bioRxiv - Genomics 2021Quote: ... samples were purified with 45 µl of Agencourt AMPure XP magnetic beads with a sample to beads ratio of 3:2 (Beckman Coulter), normalized by Qubit quantification and pooled to generate a 4 nM library ...
-
bioRxiv - Systems Biology 2024Quote: ... After cleanup with AMPure XP Reagent (Beckman Coulter, 1:1 ratio beads:PCR), iPCR amplification was carried out with KAPA HiFi HotStart ReadyMix (Roche) ...
-
bioRxiv - Molecular Biology 2023Quote: ... and purified using 1:1 (vol: vol) AMPure XL beads (Beckman Coulter). cDNA prepared as above was end-prepped using NEBNext Companion Module for Oxford Nanopore Technologies Ligation Sequencing (NEB ...
-
bioRxiv - Neuroscience 2023Quote: ... 7-color cell sorter using Summit software (Beckman Coulter Life Sciences). The sorted RFP+ only and RFP+/GFP+ cell populations were collected and replated on coverslips with pre-seeded HA cells in NDM ...
-
bioRxiv - Biochemistry 2020Quote: ... to a final volume of 1 ml and then further centrifuged (120000 g, 4°C, 2 h) in a TLA-120.2 rotor (Beckman Coulter, Brea, CA). Pellets were washed twice with 4°C cold PBS and centrifuged (120000 g ...
-
Micro RNAs are minor constituents of extracellular vesicles and are hardly delivered to target cellsbioRxiv - Cell Biology 2020Quote: ... Cat no UZ-PA-38.5-1) at 100,000 g at 4 °C for 2 h in a swinging-bucket rotor (Beckman Coulter; SW28 or SW32).
-
bioRxiv - Plant Biology 2023Quote: ... DNA extracts were purified using a bead clean up method with a 2:1 ratio of Ampure XP beads (Beckman Coulter, USA) to DNA elution ...
-
bioRxiv - Genomics 2021Quote: ... then re-purified with SPRI beads with 1:1 ratio (Beckman Coulter, B23317), and eluted in 25 µl of water (Invitrogen ...
-
bioRxiv - Immunology 2023Quote: ... Bacteria cultures were collected and discarded by centrifugation for 60 min at 4,000-8,000 x g and the supernatants were subjected to high-speed centrifugation at 11,9000 x g for 2-3 h at 4°C (Beckman Coulter Optima Ultracentrifuge). The pellets containing the OMVs were washed with PBS ...
-
bioRxiv - Cancer Biology 2020Quote: ... and NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 1 E7335S, Index Primers Set 2, E7500S) with Agencourt AMPure XP magnetic beads (Beckman coulter A63881) exactly as per manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2021Quote: ... Apoptosis was analyzed using Annexin V-FITC / 7-AAD kit (Beckman Coulter) according to the manufacturer’s protocol ...
-
bioRxiv - Genomics 2022Quote: ... HIV-1 p24 was stained with Anti-HIV-1 p24-PE (KC57) (Beckman Coulter) for 30 min on ice and washed again 3 times before measuring on the BD FACSCelesta™.
-
bioRxiv - Microbiology 2021Quote: ... using anti-HIV-1 p24-PE antibody (#6604667, 1:1000 dilution) purchased from Beckman Coulter ...
-
bioRxiv - Developmental Biology 2021Quote: ... cDNA was purified using a 1:1 volumetric ratio of Ampure Beads (Beckman Coulter) and eluted in 20 ml of water ...
-
bioRxiv - Developmental Biology 2023Quote: ... cDNA was purified using a 1:1 volumetric ratio of Ampure Beads (Beckman Coulter) and eluted in 20 ml of water ...
-
bioRxiv - Genomics 2023Quote: ... The samples were further purified using Ampure XP (1:1) (A63882, Beckman Coulter, US) and diluted in 10 µl elution buffer ...
-
bioRxiv - Cancer Biology 2023Quote: ... 7AAD (1:100; Beckman Coulter) was used for dead cell exclusion ...
-
bioRxiv - Cancer Biology 2023Quote: ... 7AAD (1:100; Beckman Coulter) was used ...
-
bioRxiv - Molecular Biology 2020Quote: ... bound antibodies were visualized using 100 µL of a 1:100 dilution of goat F(ab’)2 anti-human IgG coupled to phycoerythrin (Beckman Coulter, Brea, CA, USA) at 4°C for 30 min ...
-
bioRxiv - Biochemistry 2020Quote: Sedimentation velocity experiments were performed using a ProteomeLab XL-1 analytical ultracentrifuge with the use of an AN60-Ti rotor and Epon-2 channel centerpieces (Beckman Coulter, Brea, CA, USA). Protein samples were diluted with SANS buffer to concentrations yielding an absorbance of 0.5 at 280 nm as measured with an 8454 UV–Vis spectrophotometer (Agilent ...
-
bioRxiv - Cell Biology 2023Quote: ... where OD550 = 1 corresponds to ∼ 1 × 106 cells ml -1 (David L. Spector, 1998) or a Z1 Coulter Counter (Beckman Coulter Inc., Indianapolis, Indiana). Cultures were generally used during the log phase of cell growth (cell density 2-4 × 105 cells ml-1).
-
bioRxiv - Cell Biology 2023Quote: ... where OD550 = 1 corresponds to ∼ 1 × 106 cells ml -1 (David L. Spector, 1998) or a Z1 Coulter Counter (Beckman Coulter Inc., Indianapolis, Indiana). Cultures were generally used during the log phase of cell growth (cell density 2-4 × 105 cells ml-1) ...
-
bioRxiv - Microbiology 2023Quote: ... Extracellular vesicles were isolated by ultracentrifugation (one hour at 100,000xg, Beckman Coulter The Optima™ MAX-XP ...
-
bioRxiv - Neuroscience 2021Quote: ... and reactions were cleaned with Agencourt AMPure XP beads (1:1 volume; Beckman Coulter, A63881) retaining first-strand cDNA ...
-
bioRxiv - Cancer Biology 2020Quote: ... The libraries were purified in a 1:1 volumetric ratio of Ampure beads (Beckman Coulter), Pippin size selection was performed between 200-1000bp (Sage Science) ...
-
bioRxiv - Cell Biology 2021Quote: 1 mL samples of culture were diluted 1:100 with Isoton II Diluent (Beckman Coulter) and measured on a Beckman Multisizer 3 Coulter Counter to produce a histogram of the population’s cell volumes ...
-
bioRxiv - Cancer Biology 2020Quote: ... Excess adapter was removed using a 1:1 volumetric ratio of Ampure beads (Beckman Coulter). Bisulfite conversion was performed using the EZ-DNA methylation kit (Zymo ...
-
bioRxiv - Neuroscience 2023Quote: ... The scWGA products were then cleaned with AMPure XP beads (Beckman Coulter; 1:1 ratio).