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Citations for Beckman :
51 - 100 of 422 citations for 6 Benzyloxy 1H indole 2 boronic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2024Quote: ... The second PCR contained 6 µL of purified PCR product (using SPRIselect beads; Beckman Coulter Life Sciences ...
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bioRxiv - Molecular Biology 2021Quote: ... triglycerides and non-esterified fatty acids using a serum analyzer (AU480 Chemistry analyzer Beckman Coulter). Plasma levels of leptin and insulin were assessed using ELISAs used according to the manufacturer’s instructions (R&D Systems ...
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bioRxiv - Immunology 2020Quote: ... nucleic acids were extracted from sorted single cell using RNAClean XP SPRI beads (Beckman Coulter), and RNA was hybridized first using RT primer (/5BiosG/AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT TVN ...
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bioRxiv - Cell Biology 2024Quote: ... 6-8 PCR reactions were pooled and purified with AMPure XP SPRI Reagent (Beckman Coulter), first at 0.6x and then at a 1x volumetric ratio ...
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bioRxiv - Cell Biology 2022Quote: ... Equal volumes were distributed into 6 ultra-clear thin wall tubes (Beckman Coulter Cat. 344058) and ultra-centrifuged at 50,000 G for 2 hours at 160C in a Beckman Coulter Optima XE ultracentrifuge to precipitate lentiviral particles ...
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bioRxiv - Neuroscience 2024Quote: ... 20.000 rpm for 2 hours (Beckman). Collected viral particles were then suspended in DMEM and stored to –80°C ...
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bioRxiv - Cell Biology 2022Quote: ... Dounced cells were centrifuged at 228 g (1,000 rpm, GH-3.8 rotor; Beckman Coulter GS-6) for 5 min at 4°C to pellet nuclei and other fragments ...
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bioRxiv - Microbiology 2023Quote: ... Plasma levels of non-esterified fatty acids were measured using a UniCel DxC600 clinical analyzer (Beckman Coulter).
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bioRxiv - Immunology 2020Quote: ... Access SARS-CoV-2 IgG (Beckman Coulter), and LIAISON SARS-CoV-2 S1/S2 IgG (DiaSorin) ...
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bioRxiv - Molecular Biology 2022Quote: ... 6 mM β-mercaptoethanol) and spun at 43,000 rpm (214,000 × g) in a Type 45Ti rotor (Beckman) for 19 hours at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... and the post-nuclear supernatant was loaded onto an 8-step discontinuous sucrose density gradient (HEPES-buffered 0.2–2 M sucrose) and centrifuged at 55,000 rpm for 2h at 4 °C using an MLS50 rotor (Beckman Coulter). Extracellular acidification rate (ECAR ...
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bioRxiv - Neuroscience 2021Quote: ... KG, Staufen, Germany) (position 6, 4 × 5-s bursts) and subsequently centrifuged at 48,000g at 4°C (Beckman Avanti J-251 Ultracentrifuge ...
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bioRxiv - Microbiology 2021Quote: ... lysates were placed on top of a 20-50% sucrose gradient and sedimented at 35,000 rpm (218,000x g) for 6 hours at 4°C on SW40Ti rotor (Beckman). For flotation ...
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bioRxiv - Cell Biology 2023Quote: ... 10% and 6% and ultracentrifuged at 187,000xg for 1:30h at 4°C in Sw32.1Ti rotor (Beckman Coulter). Afterwards ...
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bioRxiv - Genetics 2024Quote: 2 mL AMPure XP beads (Beckman Coulter, A63882) were mixed thoroughly by vortexing ...
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bioRxiv - Microbiology 2024Quote: ... the solution was neutralised by the dropwise addition of 1 M hydrochloric acid centrifuged at 34,000 g (JA-20 rotor, Beckman) at 20 °C for 30 minutes to yield P5 and S5 ...
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bioRxiv - Biochemistry 2021Quote: ... 2 mM DTT) and centrifuged for 2 hours and 15 minutes at 39000 rpm at 4°C (Beckman Coulter TLS55 rotor). Fractions corresponding to the large mitochondrial subunit were collected and subjected to buffer exchange (25 mM HEPES-KOH pH = 7.5 ...
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bioRxiv - Immunology 2022Quote: CD27+ IgM- B cells cross-binding to SARS-CoV-1 and SARS-CoV-2 RBD were sorted from SARS-CoV-2 vaccinated SARS convalescents with MoFlo Astrios EQ Cell Sorter (Beckman Coulter). Briefly ...
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bioRxiv - Immunology 2021Quote: Cells were analyzed using 4-to 6-color direct immunofluorescence staining on a Cytoflex LX or S (Beckman Coulter) flow cytometer ...
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bioRxiv - Cancer Biology 2021Quote: ... Libraries were further amplified 4–6 PCR cycles as described [43] and purified using AMPure XP beads (Beckman Coulter). ATAC-seq libraries were subsequently sequenced 2 x 75 bp on an Illumina NextSeq500.
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bioRxiv - Microbiology 2020Quote: ... filtered through 45 μm-pore size filters and concentrated onto a 20% sucrose cushion by ultracentrifugation (24,000 rpm, 2h, 6°C) using a SW32 rotor (Beckman). HIV-1-based lentiviral particles were produced by co-transfecting 293T cells with packaging (psPAX2) ...
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bioRxiv - Genomics 2020Quote: ... eluted in 6 μl TE and size-selected using a 0.9X volume of Ampure XP Beads (Beckman Coulter A63882) to remove excess oligos.
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bioRxiv - Immunology 2021Quote: ... Soluble CuMV-CP-containing protein solution was separated from the cellular proteins by ultracentrifugation in a sucrose gradient (20%-60% sucrose; ultracentrifugation at 25,000 rpm for 6 hours at 5°C [SW28 rotor, Beckman]). After dialysis CuMVTT-containing gradient fractions ...
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bioRxiv - Microbiology 2022Quote: ... The gradient was spun at 45,000 rpm (~200,000 x g) for 6 hours at 4 °C in a SW60Ti rotor (Beckman Coulter). Successive 0.4 mL fractions were collected by pipetting and their densities measured ...
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bioRxiv - Cell Biology 2024Quote: ... 36 mL of media per condition was subject to ultracentrifugation at 100,000 x g for 6 hours at 4 °C in a SW-32-Ti swinging bucket rotor (Beckman). The media was decanted and 500 μL PBS was added to the pellet and left to resuspend overnight at 4 °C ...
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bioRxiv - Microbiology 2021Quote: ... calibrating with 2-micron latex bead standard (Beckman Coulter) diluted in 18% BSW as the electrolyte ...
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bioRxiv - Microbiology 2020Quote: ... for 5 minutes using a FIBERLite F10BCI-6×500y rotor (Piramoon Technologies) in an Avanti JXN-30 centrifuge (Beckman Coulter). The clarified cell lysate was further centrifuged in a JS-24.38 rotor (Beckman Coulter ...
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bioRxiv - Biophysics 2022Quote: SpeMreBs with a volume of 200 μL were polymerised for 1 to 6 h and centrifuged (100,000 rpm, 23°C, 120 min) in a TLA-100 rotor (Beckman Coulter). The pellet was resuspended in 200 μL of water ...
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bioRxiv - Biochemistry 2022Quote: ... The lysed cells were centrifuged at 41399 g and 6 °C and for 1 hour (Beckman Avanti J-25 centrifuge) to obtain the cytoplasmic protein extract.
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bioRxiv - Developmental Biology 2022Quote: ... Supernatant was then filtered using 0.22 µm filter unit and divided into 6 Ultra-Clear tubs (Beckman Coulter – Ref.344058). Tubes were ultra-centrifuged at 31000 G for 1h30 at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... were mixed 1:1 with liposomes (6 mg/ml) and floated for 2h at 55 000 rpm in a TLS-55 rotor (Beckman) at 25°C through a sucrose gradient ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were seeded into 6-well plates at a concentration of 4×105 cells per well and counted cumulatively with a Coulter counter (Beckman) every three days for two weeks ...
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bioRxiv - Biochemistry 2024Quote: ... The indicated cell lines were seeded into 6 well plates and then trypsinized and cell number counted using a Z Coulter Counter (Beckman). Approximately 200 cells were seeded into 60 mm dishes and exposed 25 ...
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bioRxiv - Plant Biology 2024Quote: ... The mixture was then centrifuged for 20 min at 9,700 g at 6°C using an Avanti J-E centrifuge and a JLA-16.250 rotor (Beckman Coulter, USA). The supernatant was separated and centrifuged for 2.5 h at 200,000 g at 6°C using an Optima XPN-90 ultracentrifuge and a Type 45 Ti rotor (Beckman Coulter ...
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bioRxiv - Plant Biology 2024Quote: ... The suspension was then centrifuged for 20 min at 9,700 g at 6°C using an Avanti J-E centrifuge and a JLA-16.250 rotor (Beckman Coulter, USA). The supernatant was separated and centrifuged for 3 h at 200,000 g at 6°C using an Optima XPN-90 ultracentrifuge with a Type 45 Ti rotor (Beckman Coulter ...
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bioRxiv - Microbiology 2022Quote: ... for 90 min or for the 6-well format at 44,000 rpm using a Beckman TLA-55 fixed angle-rotor (Beckman Coulter) for 45 min at 4°C ...
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bioRxiv - Biophysics 2023Quote: SpeMreBs in buffer S polymerized at room temperature with a volume of 200 μL were polymerized for 1–6 h and centrifuged (100,000 rpm at 23 °C for 120 min) in a TLA-100 rotor (Beckman Coulter) (2) ...
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bioRxiv - Cell Biology 2023Quote: ... down to 6 ml and ultracentrifuged at 10,000xg for 16 min at 4°C in MLA-80 rotor (Beckman Coulter). The pellet was resuspended and washed with PBS at the same conditions (volume ...
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bioRxiv - Molecular Biology 2024Quote: ... The densest solution (3.6 ml) was first placed in a 38.5 ml ultracentrifuge tube (Open-Top Thinwall Polypropylene Tube, 25 × 89mm, Beckman Coulter) and frozen for 15 minutes at -80°C ...
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bioRxiv - Bioengineering 2024Quote: ... the caveosphere mixture was layered onto a 20%–80% discontinuous sucrose gradient and subjected to velocity gradient centrifugation (120,000g, 6 hours, SW 41 Ti Swinging-Bucket Rotor, Beckman Coulter). Twenty fractions were collected from the bottom of the tube ...
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bioRxiv - Molecular Biology 2021Quote: ... the resin was spun down by centrifugation and nucleic acids were purified from supernatant with AMPure XP magnetic beads (Beckman Coulter). As a control ...
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bioRxiv - Genetics 2023Quote: ... the cell pellet was resuspended in PBS supplemented with 2 % FCS and nucleated cells were counted in methylene blue with 3 % acetic acid on a Vi-Cell XR cell viability counter (Beckman Coulter). 10 × 106 bone marrow cells were resuspended in 200 μl of PBS supplemented with 2 % FCS containing the following antibody solution ...
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bioRxiv - Cancer Biology 2022Quote: ... Nucleic acid size selection to enrich for fragment sizes between 200-500bp was conducted using SPRIselect (Beckman Coulter Life Sciences, B23318). Samples were pooled and sequenced on NextSeq2000 using TruSeq ChIP and Swift Bioscience Accel-NGS 2S Plus DNA Library Prep Kits and paired-end sequencing ...
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bioRxiv - Developmental Biology 2021Quote: ... inhibin B and AMH/MIS by 2-site ELISA (Beckman Coulter and Beckman Coulter Gen II ...
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bioRxiv - Immunology 2022Quote: ... Tagmented DNA was isolated using 2× SPRI Beads (Beckman Coulter) and amplified via PCR ...
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bioRxiv - Cell Biology 2020Quote: Interstitial macrophages (CD45+CD64+Siglec-F−CD11bhigh) or alveolar macrophages (CD45+CD64+Siglec-F+CD11blow) were isolated from C57BL/6 by MOFLO system (Beckman Coulter). Isolated macrophages were cultured for 24 hrs in RPMI-1640 medium containing 10% FBS and 50μM 2-mercaptoethanol with or without GM-CSF (10 ng/ml).
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bioRxiv - Cancer Biology 2020Quote: ... cells were grown for 4-6 days and counted using an Incucyte (Essen Biosciences) or a Z2 Coulter Counter (Beckman Coulter). Small molecule dose-response curves were printed using an D300e Digital Dispenser (HP Specialty Printing Systems ...
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bioRxiv - Cell Biology 2022Quote: ... Resulting libraries were then pooled in sets of 6 (approximately 36,000 beads per pool) and products purified by Agencourt AMPure XP beads (Beckman Coulter, A63881) with a 0.6x volume wash followed by a 0.8x volume wash ...
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bioRxiv - Immunology 2023Quote: ... Resulting libraries were then pooled in sets of 6 (approximately 36,000 beads per pool) and products purified by Agencourt AMPure XP beads (Beckman Coulter, A63881) with a 0.6x volume wash followed by a 0.8x volume wash ...
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bioRxiv - Plant Biology 2023Quote: ... the vesicle containing fraction was dispersed in 350 µl fractions in 1.5 mL (9.5 x 38 mm) in 6 polypropylene microfuge tubes (Beckman Coulter, Cat. # 357448) and the volume in each tube was brought up to 1,500 µl using 20 mM Tris-HCl pH 6.0 ...