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Citations for Beckman :
901 - 950 of 1603 citations for tert Butyl trans 17 bromo 4 7 10 13 tetraoxa 15 heptadecenoate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2024Quote: ... Lysis was accomplished via douncing and the lysate was clarified via centrifugation (183,960 x g, 88 min, 4°C) in a Type 70Ti rotor (Beckman). 2 mL of IgG Sepharose 6 Fast Flow beads (Cytiva ...
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bioRxiv - Cell Biology 2024Quote: ... and the lysate was cleared twice by centrifugation at 40,000 rcf for 20 min each at 4°C using a JA 25.50 rotor (Beckman Coulter). The cleared lysate was incubated with 2 mL Ni-NTA resin (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... 36 mL of media per condition was subject to ultracentrifugation at 100,000 x g for 6 hours at 4 °C in a SW-32-Ti swinging bucket rotor (Beckman). The media was decanted and 500 μL PBS was added to the pellet and left to resuspend overnight at 4 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... samples were loaded based on equal total RNA onto a 10%–50% sucrose gradient and subjected to ultracentrifugation (187,813 g for 1.5 hours at 4 °C) using a SW 41 Ti rotor (Beckman Coulter). Subsequently ...
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bioRxiv - Bioengineering 2024Quote: ... The virus was then pelleted by centrifugation at 4°C for 45 min at 25,000 rpm in a sw28 rotor (Beckman) and gently suspended in dilution buffer (150 mM NaCl ...
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bioRxiv - Cancer Biology 2024Quote: ... which was then centrifuged at 100,000 × g for 18 hours at 4°C (344060, SW 40 Ti, Beckman Coulter) for further purification ...
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bioRxiv - Cell Biology 2024Quote: ... All ultracentrifugation steps will be performed at 4°C using a SW41 Ti rotor (Beckman Coulter, Brea, CA, USA).
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bioRxiv - Cell Biology 2024Quote: ... The 100,000xg SEV-containing pellets were resuspended in 3 ml sterile cold PBS and repelleted at 100,000xg for 4 hrs in a TLA110 rotor (Beckman). SEVs were analyzed for size and number by nanoparticle tracking (ZetaView ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Ribosomes were pelleted by centrifugation at 27,000 rpm (57,000 xg) for 16 h at 4 °C in a Ti-45 rotor (Beckman-Coulter). Ribosomes pellets were then resuspended in dissociation buffer (20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Cell Biology 2024Quote: ... Lysates were clarified by centrifugation at 20,000 x g for 45 mins at 4°C in a JA25.50 rotor (Beckman Coulter). Clarified lysates were applied to a gravity flow column several times over 2 ml Ni-NTA resin (Sigma ...
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bioRxiv - Cancer Biology 2021Quote: ... Dead cells were excluded using the viability dye 7AAD (1:10; Beckman Coulter, A07704). Compensation was performed using mouse IgG CompBeads (BD Biosciences ...
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bioRxiv - Immunology 2021Quote: ... The levels of IL-10 were evaluated in NAVIOUS EX flow cytometer (Beckman Coulter), and the results were analyzed using Kaluza ...
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bioRxiv - Cell Biology 2020Quote: ... followed by serial centrifugation at 100’000 g for 120 min at 10 °C (Beckman fixed-angle TL110 rotor ...
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bioRxiv - Cancer Biology 2021Quote: ... They were then diluted 1:10 in Coulter Isoton II diluent (Beckman Coulter, 8546719) and cell diameters determined using a particle sizing and counting analyser (Multisizer 4 Coulter counter ...
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bioRxiv - Biochemistry 2022Quote: ... The cultures were harvested by centrifugation (JLA 8.1000 rotor, Beckman, 3000 rpm, 10 min), washed once with PBS ...
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bioRxiv - Microbiology 2021Quote: ... Cells were centrifuged (Avanti JXN-26 Beckman Coulter Centrifuge, Beckman Coulter JA-10 rotor) at 7,000 x g for 0.5 h at 4°C ...
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bioRxiv - Systems Biology 2022Quote: ... Flow cytometry data were acquired using a 10-color Navios flow cytometer (Beckman Coulter) and the Kaluza Flow Cytometry software (Beckman Coulter ...
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bioRxiv - Microbiology 2020Quote: ... the VLPs were concentrated by ultracentrifugation (40,000 rpm, 2h, 10°C, Beckman SW41 rotor), resuspended in buffer A and observed by TEM ...
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bioRxiv - Immunology 2020Quote: ... Whole milk was centrifuged at 22°C for 10 minutes at 3,000 g (Beckman Coulter Allegra X-12R ...
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bioRxiv - Immunology 2021Quote: ... The 10 cDNA plates were cleaned-up using AMPure XP beads (Beckman Coulter #A63881), deep 96 well plates (Abgene #AB-0765 ...
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bioRxiv - Immunology 2024Quote: ... Flow cytometry was performed on a 10-color flow cytometer Gallios™ (Beckman Coulter) and FACS was done on MofloTM (Beckmann Coulter ...
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bioRxiv - Biochemistry 2023Quote: ... Liposomes were sedimented via ultracentrifugation for 10 min at 70,000 rpm (TLA-100, Beckman) and 20°C ...
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bioRxiv - Physiology 2024Quote: ... Chylomicrons were floated by centrifugation (SW 32Ti, Beckman, 20K rpm, 30 min, 10° C) and removed by aspiration ...
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bioRxiv - Developmental Biology 2024Quote: ... after which bacteria were pelleted (Beckman Coulter JS-4.2 rotor, 10 min, 4000 rpm) and diluted in a solution of 5% sucrose to reach OD600=25.
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bioRxiv - Biochemistry 2021Quote: ... 10 U/mL SuperASE-In and was ultracentrifuged at 100,000 × g for for 16 h at 4 °C in a SW Ti41 rotor (Beckman Coulter). After removing the supernatant ...
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bioRxiv - Biochemistry 2021Quote: ... The lysate was clarified by ultracentrifugation at 30,000 × g 30 min at 4°C using a Ti45 rotor (Beckman Coulter) twice ...
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bioRxiv - Biophysics 2020Quote: ... Escherichia coli BL21 cells overexpressing α-synuclein were collected by centrifugation (20 min, 4000 rpm, 4 °C) in a JLA-8.1000 rotor in a Beckman Avanti J25 centrifuge (Beckman Coulter), resuspended in lysis buffer (10 mM Tris ...
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bioRxiv - Biophysics 2020Quote: ... and centrifuged at 45000 x g for 60 min at 4 °C in the Avanti J-26S ultracentrifuge (JA-30.50Ti rotor, Beckman Coulter). The cleared cell lysate was incubated in a lysis buffer-equilibrated Ni-NTA column (HisPur Ni-NTA Superflow Agarose ...
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bioRxiv - Cell Biology 2020Quote: ... Crude mitochondria were loaded on top of the chilled gradient and separated at 100,000 Å∼ g for 1 h at 4°C (SW41 rotor; Beckman). Intact purified mitochondria were recovered from a brown band at around 16% Histodenz™ concentration ...
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bioRxiv - Cell Biology 2020Quote: ... and the gradient was centrifuged for 60 min at 24,700 × g at 4 °C using a TLS-55 swinging bucket rotor (Beckman Coulter). Purified mouse POS were resuspended in DMEM/Ham’s F-12 containing 2.5% sucrose ...
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bioRxiv - Cell Biology 2020Quote: ... lysed by sonication (UD-201 ultrasonic disrupter, Tomy Seiko), and centrifuged at 50,000 rpm (70 min, 4°C) with a 70 Ti rotor (Beckman Coulter). GST-His6-tagged LC3B-His12 and GATE-16-His12 proteins in the supernatants obtained were isolated by mixing with COSMOGEL GST-Accept beads (50% slurry ...
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bioRxiv - Immunology 2021Quote: ... the culture medium containing the lentiviral particles was collected and ultracentrifuged at 25,000 rpm for 90 min at 4 °C using a Beckman SW28 ultracentrifuge rotor (Beckman Coulter) for the obtention of concentrated virus ...
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bioRxiv - Neuroscience 2021Quote: ... PMCA products were centrifuged at 45,000 rpm for 2.5 h at 4 °C (Optima™ Max Ultracentrifuge, Beckman Coulter, USA), the supernatants discarded and the pellets resuspended in an equal volume of PBS.
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bioRxiv - Molecular Biology 2021Quote: ... 2 mM DTT and centrifuged for 16 h at 34,000 rpm at 4°C for 16 h using an SW60 Ti rotor (Beckman Coulter). The fractions were subjected to SDS-PAGE and Coomassie blue staining.
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bioRxiv - Molecular Biology 2021Quote: ... The samples were centrifuged at 34,000 rpm at 4°C for 16 h using an SW60 Ti rotor (Beckman Coulter). Subsequently ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 mM DTT and centrifuged for 16 h at 34,000 rpm at 4°C for 16 h using an SW60 Ti rotor (Beckman Coulter). The fractions ...
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bioRxiv - Molecular Biology 2020Quote: ... Gradients were centrifuged at 39 000 rpm for 2.5 h at 4°C in an Optima L-100XP ultracentrifuge (Beckman– Coulter) using the SW41Ti rotor with brake ...
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bioRxiv - Cell Biology 2020Quote: ... and centrifuged at 45000 x g for 60 min at 4 °C in the Avanti J-26S ultracentrifuge (JA-30.50Ti rotor, Beckman Coulter). The cleared cell lysate was incubated in a lysis buffer-equilibrated Ni-NTA column (HisPur Ni-NTA Superflow Agarose ...
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bioRxiv - Molecular Biology 2020Quote: ... The total bacterial lysate was centrifuged at 48,384 x g at 4 °C for 1 hr (Beckman JA-25.50 rotor), and the supernatant was applied to a 5 mL HisPur Ni-NTA resin (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2021Quote: ... The unbound cyt c was separated from liposome-bound cyt c by ultracentrifugation at 435,000 g for 2 hours at 4 °C in an Optima TLX ultracentrifuge with a TLA-100 rotor (Beckman Coulter). Immediately after the ultracentrifugation ...
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bioRxiv - Biochemistry 2020Quote: ... actins were cleared by highspeed centrifugation at 316,613 x g for 1 hour at 4°C in a TLA100 rotor (Beckman Coulter). Only the top 80% of the supernatant was collected to perform TIRF microscopy ...
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bioRxiv - Developmental Biology 2020Quote: ... Separation of testis cell populations was performed by centrifugal elutriation using a JE-5.0 elutriation system and a 4-ml standard elutriation chamber (Beckman Coulter). The assembly of the system followed the manufacturer’s instruction ...
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bioRxiv - Bioengineering 2020Quote: ... the EV pellets were resuspended in 3 ml 0.32 M sucrose (diluted in PBS) solution and centrifuged again at 100,000xg for 60 minutes at 4°C (SW60 rotor, Beckman Coulter). Sucrose supernatant was removed and pellet was resuspended in 500 μl phosphate-buffered saline containing 0.01% bovine serum albumin (0.2 μm filtered ...
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bioRxiv - Biophysics 2021Quote: Pooled seminal plasma from stallions was centrifuged for 30 min at 10,000g and 4°C (Beckman Coulter, BC - rotor SW32Ti) to pellet cell debris and larger molecular complexes ...
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bioRxiv - Cancer Biology 2021Quote: ... Tubes were then spun at 10,000 x g for 20 minutes at 4 °C in an ultracentrifuge (Beckman, L8-70M). The supernatant was filtered using 0.2 μM syringe filters (Millipore Sigma ...
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bioRxiv - Cancer Biology 2021Quote: ... The pellet was washed with PBS and ultracentrifuged at 100,000 × g 1h at 4°C to obtain small vesicles (TL-100 Ultracentrifuge, Beckman Coulter). The final pellet was resuspended in PBS for subsequent experiments ...
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bioRxiv - Biochemistry 2020Quote: ... The eluted sample was collected by centrifugation at 2000 rpm for 1 minutes 4 °C in a F241.5P rotor using a 149 Microfuge 22R centrifuge (Beckman Coulter). One aliquot of the eluted sample was resolved on SDS-PAGE ...
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bioRxiv - Biochemistry 2020Quote: ... 20% FBS containing RPMI was ultracentrifuged for 18□hours at 100,000□g at 4□°C in a 45Ti fixed angle rotor using polycarbonate tubes (both from Beckman Coulter). After ultracentrifugation ...
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bioRxiv - Microbiology 2020Quote: ... the sample was centrifuged for 16 h at 4°C and 100,000 rcf using a type 70 Ti rotor (Beckman Coulter). The pellet was gently washed with 500 μl of storage buffer (lysis buffer C + 10% (v/v ...
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bioRxiv - Microbiology 2021Quote: ... The density gradient centrifugation was performed at 141,000 g and 4 °C for 20 h in a SW28 swing rotor (Beckman Coulter). A total of 18 fractions were collected starting from the top.