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Citations for Beckman :
901 - 950 of 1127 citations for Rat EGF Latrophilin And Seven Transmembrane Domain Containing Protein 1 ELTD1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2024Quote: ... They were transduced via spinfection at 1000 rpm for 1 hour at 30 °C (Beckman Coulter, Allegra X-14R), followed by overnight incubation ...
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Syntaxin-2 inhibits excessive phagocytic uptake but promotes phagolysosomal clearance in macrophagesbioRxiv - Cell Biology 2024Quote: ... The samples were then centrifuged at 100,000 g for 1 hr at 4°C using a SW40Ti rotor (Beckman). Phagosomes were recovered from the interface of 10 and 25% sucrose (Fig ...
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bioRxiv - Plant Biology 2024Quote: ... the supernatant after the 40,000 g spin was transferred to ultracentrifuge tubes and centrifuged at 100,000 g for 1 h at 4 °C (TLA100.3 rotor, Optima TLX Ultracentrifuge; Beckman Coulter). P40 and P100-P40 pellets were resuspended in cold and filtered VIB or 100 mM Tris pH 7.4 and either used immediately or stored at –80 °C until further use.
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bioRxiv - Plant Biology 2024Quote: ... tubes and centrifuged at 40,000 g for 1 h at 4 °C (TLA100.3 rotor, Optima TLX Ultracentrifuge; Beckman Coulter). Where indicated ...
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bioRxiv - Immunology 2024Quote: ... Cells were washed and resuspended in PBS 1% FBS 2mM EDTA and run on a Cytoflex LX (Beckman Coulter).
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bioRxiv - Microbiology 2024Quote: ... For p24 staining (RD1-conjugated mouse anti-p24 clone KC57, cat# 6604667, 1:100 dilution, Beckman Coulter, Brea, CA), cells were fixed and permeabilized using the Cytofix/Cytoperm kit (BD Biosciences ...
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bioRxiv - Microbiology 2024Quote: ... Amplicons were visualized on a 1% agarose gel and purified with Agencourt AMPure XP beads (Beckman Coulter, Brea, CA). The concentration of purified amplicons was quantified using the Qubit High Sensitivity dsDNA kit and a Qubit Fluorometer (Invitrogen) ...
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bioRxiv - Biophysics 2020Quote: ... Very small amounts of fibrillar species formed during this time were removed by ultracentrifugation for 1 h at 90000 rpm (Optima TLX Ultracentrifuge, Beckman Coulter ...
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bioRxiv - Cell Biology 2020Quote: ... Crude mitochondria were loaded on top of the chilled gradient and separated at 100,000 Å∼ g for 1 h at 4°C (SW41 rotor; Beckman). Intact purified mitochondria were recovered from a brown band at around 16% Histodenz™ concentration ...
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bioRxiv - Microbiology 2019Quote: ... and centrifuged at 40,008 × g for 1 hour at 4ºC in an Beckman L-100 XP ultracentrifuge with the SW 41 Ti swing rotor (Beckman Coulter). Following centrifugation ...
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bioRxiv - Biochemistry 2019Quote: ... the cells were dissolved in the same buffer as for TtBac-WT 1-123 and spun for 30 min at 9k rpm in a Ti45 rotor (Beckman). The pellet was dissolved in the same buffer and from here onwards the same protocol as for TtBac-WT 1-123 was used.
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bioRxiv - Molecular Biology 2020Quote: ... The total bacterial lysate was centrifuged at 48,384 x g at 4 °C for 1 hr (Beckman JA-25.50 rotor), and the supernatant was applied to a 5 mL HisPur Ni-NTA resin (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... actins were cleared by highspeed centrifugation at 316,613 x g for 1 hour at 4°C in a TLA100 rotor (Beckman Coulter). Only the top 80% of the supernatant was collected to perform TIRF microscopy ...
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bioRxiv - Bioengineering 2021Quote: ... Cultures were grown in a MTP-48-BOH 1 FlowerPlate in a BioLector Pro (both Beckman Coulter Life Sciences, USA) at 1400 rpm ...
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bioRxiv - Biochemistry 2020Quote: ... The eluted sample was collected by centrifugation at 2000 rpm for 1 minutes 4 °C in a F241.5P rotor using a 149 Microfuge 22R centrifuge (Beckman Coulter). One aliquot of the eluted sample was resolved on SDS-PAGE ...
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bioRxiv - Biochemistry 2020Quote: ... After increasing the NH4Cl concentration of the supernatant to 1 M to dissociate bS1 from 70S ribosomes the sample was centrifuged in a Type 70 Ti rotor (Beckman) at 60,000 g for 2 hours ...
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bioRxiv - Systems Biology 2020Quote: ... GFP productivity was determined by measuring the GFP signal (FL-1 channel) of cells by flow cytometry (Gallios, Beckman Coulter).
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bioRxiv - Microbiology 2021Quote: ... and stained intracellularly using PE-conjugated mouse anti-p24 mAb (clone KC57; Beckman Coulter, Brea, CA, USA; 1:100 dilution) in combination with Nef or Vpu rabbit antisera (1:1000 dilution) ...
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bioRxiv - Genomics 2021Quote: ... 1 mM EDTA (corresponding to a refractive index of 1.3700) and loaded into OptiSeal polypropylene centrifuge tubes (Beckman Coulter, 362185). Centrifugation was performed in a near-vertical NVT-90 rotor (Beckman Coulter ...
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kinesin recruitment by adapter SKIP on melanosomes is dynamically controlled by LC3B phosphorylationbioRxiv - Cell Biology 2021Quote: ... The post nuclear supernatant was collected and separated on a stepwise sucrose density gradient and centrifuged at 100,000 g for 1 hour at 4°C in a swing-out rotor (Beckman coulter). The sucrose density gradients comprised 0.25 ...
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bioRxiv - Biophysics 2022Quote: SpeMreBs with a volume of 200 μL were polymerised for 1 to 6 h and centrifuged (100,000 rpm, 23°C, 120 min) in a TLA-100 rotor (Beckman Coulter). The pellet was resuspended in 200 μL of water ...
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bioRxiv - Molecular Biology 2022Quote: ... Stained cells were resuspended in sort buffer at 1 × 106/ml for fluorescence analysis on a Cytoflex S cell analyser (Beckman). SARS-CoV-2 Spike variants were generated by site-directed mutagenesis with Q5 polymerase (New England Biolabs ...
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bioRxiv - Biochemistry 2022Quote: ... and the debris and membranes were removed by ultracentrifugation at 205,000 g for 1 hr at 4 °C (rotor 45 Ti, Beckman Coulter). The supernatants were applied to IMAC with Ni2+-NTA-agarose resin (Qiagen ...
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bioRxiv - Molecular Biology 2022Quote: ... The sucrose gradient was centrifuged at 24,400 rcf for 1 h at 4°C in a SW41Ti Swinging-Bucket Rotor (Beckman Coulter), and nuclei at the bottom of the gradient were resuspended in PBS ...
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bioRxiv - Biochemistry 2022Quote: ... The lysed cells were centrifuged at 41399 g and 6 °C and for 1 hour (Beckman Avanti J-25 centrifuge) to obtain the cytoplasmic protein extract.
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bioRxiv - Cell Biology 2022Quote: ... were mixed 1:1 with liposomes (6 mg/ml) and floated for 2h at 55 000 rpm in a TLS-55 rotor (Beckman) at 25°C through a sucrose gradient ...
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bioRxiv - Plant Biology 2021Quote: ... The thylakoid membranes with different densities were analytically separated at 125,000 × g and 4°C for 1 h (SW 41 Ti, Beckman Coulter).
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bioRxiv - Microbiology 2021Quote: ... culture samples were diluted (1:100 or 1:1000) with 0.2-µm-filtered 18% BSW and were analysed with a Multisizer M4 Coulter cytometer (Beckman-Coulter) equipped with a 20 or 30 µm aperture tube ...
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bioRxiv - Microbiology 2020Quote: ... OBs were loaded on to a discontinuous 35–60% (w/w) sucrose gradient and centrifuged at 20000 rpm and 4°C for 1 hour in a SW41 rotor (Beckman). The band containing the OBs was removed ...
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bioRxiv - Cell Biology 2020Quote: ... After purification of the PCR product with a 1: 0.6 ratio of PCR product to AMPure XP beads (Beckman Coulter), the success of cDNA preparation was confirmed using a 2100 Bioanalyzer with High-sensitivity DNA chip (Agilent) ...
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bioRxiv - Neuroscience 2020Quote: ... The remaining supernatant (conditioned medium) was subjected to ultracentrifugation (UC) (100,000 x g 1 h) using rotors Ti45 or SW32Ti (Beckman Coulter). The pellet was rinsed with PBS and spun again using rotor SW55Ti (100,000 x g 1 h).
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bioRxiv - Neuroscience 2022Quote: ... The remaining supernatant (conditioned medium) was subjected to ultracentrifugation (UC) (100,000 x g, 1 h) using rotors Ti45 or SW32Ti (Beckman Coulter). The pellet was rinsed with PBS and spun again using rotor SW55Ti (100,000 x g ...
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bioRxiv - Neuroscience 2022Quote: ... 10 mM Tris-HCl) and centrifugated at 24,000 rpm for 1 h at 4°C in a SW41Ti Swinging-Bucket Rotor (Beckman Coulter). Supernatant was discarded and nuclei pellets were re-suspended in 800 µL of PBS ...
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bioRxiv - Biochemistry 2019Quote: ... 10,000 events were then immediately analyzed from 1 of the 4 150 µL aliquots using a CytoFLEX benchtop cytometer (Beckman). All subsequent chase time points were collected using this initial analysis as a reference ...
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bioRxiv - Biochemistry 2019Quote: ... The supernatant was then slowly mixed with an equal volume of 4 M ammonium sulphate (2 M final concentration) at 4°C for 1 hour and spun at 10 K rpm using a JA-17 rotor (Beckman). Pellets containing RAD51 were suspended in 25 ml of resuspension buffer (0.5 M KCl ...
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bioRxiv - Biophysics 2021Quote: ... Cells were lysed and the lysate was clarified by centrifugation for 1 h at 100,000g (45 Ti rotor, Beckman Coulter). The clarified lysate was incubated with Ni2+-NTA agarose resin for 1 h at 4 °C ...
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bioRxiv - Synthetic Biology 2021Quote: ... Libraries corresponding to samples to be directly compared to each other (e.g. DMSO vs UNC7040) were pooled together and purified using 1 volume of Agencourt AMPure XP (Beckman Coulter). The pooled libraries were eluted with 23 µL of elution buffer (NEXTflex ChIP-Seq kit ...
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bioRxiv - Immunology 2020Quote: ... Then the concentrated supernatant was ultracentrifuged at 110,000 g for 1 hour at 4°C (Beckman Coulter L100k, company name) to sediment the vesicular fraction into a pellet ...
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bioRxiv - Microbiology 2020Quote: ... The cell lysate was collected and centrifuged at 100,000 x g for 1 h in an ultracentrifuge (Model XL-90, Beckman Coulter). The supernatant was collected ...
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bioRxiv - Cancer Biology 2019Quote: ... All 384 single-cell libraries in each batch were pooled and cleaned up with 0.6:1 Ampure XP beads (Beckman Coulter). The multiplexed RNA-seq library was normalized to 4nM with Suspension Buffer (provided in Nextera XT kit ...
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Heatrich-BS enables efficient CpG enrichment and highly scalable cell-free DNA methylation profilingbioRxiv - Genomics 2021Quote: ... the sample was incubated at 25°C for 1 hour and then cleaned up by performing two rounds of 1.2x SPRI Select (Beckman Coulter). The sample was then subject to bisulfite conversion following the recommended protocol of Zymo EZ DNA Methylation kit (Zymo Research) ...
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bioRxiv - Genomics 2021Quote: ... The cDNA was purified using RNA XP beads purification with 1:1.8x ratio of sample to beads (Beckman Coulter, A63987) and eluted in 24.5 µl water ...
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bioRxiv - Neuroscience 2021Quote: ... and centrifuged at 250,000 g for 1 hour at room temperature with a TLA 120.2 rotor using an Optima XP benchtop ultracentrifuge (Beckman Coulter). Supernatant were collected by pipetting ...
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bioRxiv - Microbiology 2021Quote: ... bacterial cells were washed in PBS and diluted 1:5 before acquisition on a CytoFLEX benchtop flow cytometer (Beckman Coulter). For each sample ...
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bioRxiv - Genomics 2020Quote: ... This sequencing-ready NGS library was purified using 1 round of a 1X proportion of AMPure XP beads (Beckman Coulter). The final elution volume was 17 µl in Elution Buffer.
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bioRxiv - Microbiology 2021Quote: ... followed by purification with a 2.2:1 volumetric ratio of RNAClean XP solid phase reverse immobilization (SPRI) beads (A63987, Beckman Coulter). Copy numbers of resulting cDNAs were determined by limiting-dilution PCR using fluorescence-assisted clonal amplification (FCA ...
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bioRxiv - Microbiology 2020Quote: ... through a 1 ml 30% sucrose cushion (in phosphate-buffered saline, PBS, pH 7.4) in a SW41 rotor (Beckman coulter). The pelleted virus material was re-solubilized in PBS and analyzed by sodium docecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE ...
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bioRxiv - Immunology 2021Quote: ... Cells were stained with following antibody mix for 30 minutes on ice: pan-γδTCR-PE (1:10; IMMU510; Beckman Coultier) and CD69-APC (1:25 ...
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bioRxiv - Immunology 2021Quote: ... 1×106 thawed PBMCs were centrifuged (750×g for 5 minutes at 4°C) using a swinging bucket rotor (Beckman Coulter Avanti J-15RIVD with JS4.750 swinging bucket ...
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bioRxiv - Microbiology 2022Quote: ... PCR products were confirmed through visualization in 1% agarose gel and purified using AMPure XP beads (Beckman Coulter, Indianapolis, U.S.A.). PCR libraries were pooled together and sent to the Centre d’expertise et de services Genome Québec (Montréal ...