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Citations for Beckman :
901 - 950 of 1017 citations for Nipah Virus Glycoprotein G Recombinant Protein Mouse Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... supernatants were collected and centrifuged with 150.000 x g (at rav) for 30 min at 4 °C using an Optima™ MAX-XP Ultracentrifuge (Beckman Coulter; TLA120.2 rotor). Pellets were resuspended in PBS and 4 µg of primary antibody were added for 30 min ...
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bioRxiv - Biochemistry 2020Quote: ... The sample was centrifuged at 4°C for 1 hour and 40 minutes at 182,753 x g (38,000 rpm in a Beckman Coulter Type 42.2 Ti rotor).
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bioRxiv - Biochemistry 2020Quote: ... was then added to the product which passed microfluidizer and unbroken cells and large debris were eliminated by centrifugation at 4000 × g (Beckman Coulter™, USA). The supernatant containing the IMVs was collected and washed several times with resuspension buffer by centrifugation at approximately 310,000 × g at 4°C for 30 minutes (SORVALL® Centrifuges ...
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bioRxiv - Biophysics 2020Quote: ... 150 μl of the sample was ultracentrifuged at 22°C and 205000 × g for 19 min (TLA 100.3, Beckman Coulter Inc., Fullerton, California). The experiments were performed in HEPES buffer ...
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bioRxiv - Bioengineering 2021Quote: ... The samples were then centrifuged at 12,000 g for five minutes at 4°C to remove tissue debris with a Microfuge™ 22R centrifuge (Beckman Coulter, Brea, CA). The RNA was extracted using the Invitrogen PureLink™ RNA Mini Kit (Thermo Fisher ...
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bioRxiv - Bioengineering 2021Quote: ... The tubes were then centrifuged at 1000 g for 15 minutes at 4°C using a Microfuge™ 22R (Beckman Coulter, Brea, CA), and the serum was then stored at -80°C ...
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bioRxiv - Microbiology 2021Quote: ... and extracellular vesicles including exosomes and virions were pelleted by ultracentrifugation at 141,000 g at 4 °C for 2 h in a swinging-bucket rotor (SW28; Beckman Coulter, Brea, CA, USA). The pellet was resuspended in filtered PBS and centrifuged at 18,300 g and 4°C for 10 min to remove insoluble aggregates ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 mM ATP and 1 mM PMSF) and centrifuged for 15 h at 4°C and 74 400 x g (Beckman SW 32.1 Ti). From the gradient ...
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bioRxiv - Microbiology 2021Quote: ... resuspended in 150 μl 0.1 M HEPES/NAOH pH 7.0 and purified for 48 h (250,000 × g, 4 °C; Beckman Optima LE-80K, rotor SW60-Ti) using a CsCl gradient (0.45 g/ml ...
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bioRxiv - Microbiology 2019Quote: ... and the remaining 32 mL were centrifuged at 500 × g for 3 min at 4°C (Allegra X-15R, Beckman Coulter, Villepinte, France) to spin down the remaining meat fibers and debris ...
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bioRxiv - Cancer Biology 2021Quote: ... previously centrifuged for 16 h at 120,000 g to remove bovine exosomes using 40.1Ti rotor (L-80XP; Beckman Coulter®, Brea, CA, USA).) ...
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bioRxiv - Biophysics 2022Quote: ... Fractions of EV-specific density were then pooled together and subsequently concentrated via ultracentrifugation (100,000 × g, 4 h, 4 °C) through a 20% w/v sucrose cushion in a SW41 rotor (Beckman Coulter, Fullerton, CA, USA). The resulting supernatant was discarded ...
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bioRxiv - Biophysics 2022Quote: ... EVs were fractionated by OptiPrep density gradient ultracentrifugation (100,000 × g, 18 h, 4 °C) using a SW41 rotor (Beckman Coulter, Fullerton, CA, USA) through a continuous 5–40% OptiPrep (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2022Quote: ... the carboy was chilled in the cold room overnight (Figure S37D) and then harvested by centrifugation at 8,000 x g (Beckman Avanti HP-26 XPI) in 1 L bottles ...
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bioRxiv - Immunology 2022Quote: ... followed by ultracentrifugation at 110,000 × g for 90 min at 4°C on an Optima XPN -100 ultracentrifuge with SW32Ti rotor (Beckman Coulter, Brea, CA, USA). The isolated EVs was washed with PBS and followed with a secondary ultracentrifugation (110,000 × g for 90 min at 4°C) ...
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bioRxiv - Biophysics 2022Quote: ... The fractions containing monomeric peptide were pooled and centrifuged at 386000 g for 3 h in an Optima TLX ultracentrifuge equipped with a TLA 120.1 rotor (Beckman Coulter, Atlanta, GA, USA). Orthophosphoric acid or sodium phosphate were added to a final concentration of 20 mM to adjust the pH to 2.8 or 7.4 ...
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bioRxiv - Microbiology 2020Quote: ... The clarified supernatant was overlaid onto a 20% (w/v) sucrose cushion in Tris-NaCl-EDTA buffer and centrifuged at 134,000 g for 2 hours (Beckman Coulter 30 Ti rotor). The resulting viral pellet was suspended in VCM by repeat pipetting and aliquots stored in cryovials at minus 70°C until needed ...
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bioRxiv - Microbiology 2020Quote: ... EMVs were obtained by ultracentrifugation from 4 mL of filtrate at 100,000 × g (average centrifugal force) and 4°C for 2 h with an OptimaX centrifuge (Beckman Coulter, Brea, CA, USA). The pellets were resuspended in 400 μL of Dulbecco’s phosphate-buffered saline (DPBS ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... for 10 m at 4°C and pelleted the lysates at 50,000×g for 1 h at 4°C using an Optima MAX-XP ultracentrifuge (Beckman Coulter, Brea, CA, USA). We resuspended cell fractions in 500 μL water and stored the samples at 4°C until analysis ...
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bioRxiv - Microbiology 2021Quote: ... Ten milliliters of allantoic or amniotic fluid was centrifuged at maximum speed (16,000 × g) for 30 min at 4 °C using a floor ultra centrifuge (Beckman Optima L-60 Ultracentrifuge). After removal of the supernatant ...
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bioRxiv - Microbiology 2019Quote: ... the extracts were added on top of layers in LCM buffer with 45% and 5% glycerol and ultracentrifuged at 77 000 x g for 1 h at +4°C (Beckman Coulter SW41Ti rotor). Viral nucleocapsids were recovered from the bottom of the ultracentrifuge tube ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Results in LIFE PERSUADED project were reported un-adjust (μg/L) and adjusted (μg/g crea) to creatinine concentrations measured using the Jaffe method (Beckman Coulter, Brea, CA) [95 ...
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bioRxiv - Cell Biology 2020Quote: ... followed by serial centrifugation at 100’000 g for 120 min at 10 °C (Beckman fixed-angle TL110 rotor; Beckman Optima Max-TL ultracentrifuge). The so obtained P100 pellets ...
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bioRxiv - Immunology 2020Quote: ... 110 mM KCl with freshly added DTT) at 2,000 x g in a swinging bucket rotor (e.g. 4,000 rpm in a Beckman Coulter J6-MC floor model centrifuge) for 20 min at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... The supernatant fractions were collected and centrifuged at 75,600 x g for 90 min at 4 °C (Beckman JA25.50 rotor, Beckman Avanati J-25, Beckman Coulter). The resulting pellets were washed again and resuspended in 500 μl potassium phosphate buffer ...
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bioRxiv - Microbiology 2022Quote: ... 100 ml cell cultures were grown over night and harvested by centrifugation at 15,000 x g for 15 min at 4 °C (Beckman JLA-16.250 rotor, Beckman Avanti J-25, Beckman Coulter). Pellets were washed with 20 mM potassium phosphate (pH 7.5 ...
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bioRxiv - Microbiology 2022Quote: ... The clarified supernatant was collected and pelleted through a 30% (w/v) sucrose cushion at 151,000 × g (using a Beckman SW 32 Ti rotor) for 3.5 hours at 10°C ...
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bioRxiv - Microbiology 2022Quote: ... The supernatant was collected and pelleted through a 30% (w/v) sucrose cushion at 151,000 × g (using a Beckman SW 32 Ti rotor) for 3.5 hours at 10°C ...
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bioRxiv - Microbiology 2023Quote: ... cells were pelleted by centrifugation at 6,000 ×g at 4 °C for 15 min with ALLEGRA X-30R (Beckman Coulter, Brea, CA, USA). The supernatant was centrifuged at 15,000 ×g at 4 °C for 15 min with Sorvall LYNX4000 (Thermo Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... 5 ml of complete Neurobasal medium were added and centrifuged for 5 min at 335 x g (Beckman Coulter Allegra X-12R centrifuge). The complete Neurobasal medium consisted of ...
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bioRxiv - Cell Biology 2023Quote: ... supernatants were centrifuged at 100 000 x g for 70 min in ultracentrifugation using type 70 Ti rotor (Oprima XL-80K, Beckman, Brea, CA, USA) to pellet the EVs ...
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bioRxiv - Molecular Biology 2023Quote: ... aggregates and debris were removed by centrifuging at 1,000 ×g for 10 minutes at RT (Avanti J–15R; Beckman Coulter Inc., Brea, USA). Next ...
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bioRxiv - Cancer Biology 2022Quote: ... the sample was centrifuged at 268,000 ×g at 4°C for 38 min with an MLA-50 rotor (Beckman Coulter, Brea, CA, USA). The top layer and subsequent 10 fractions (fractions 1–10 ...
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bioRxiv - Plant Biology 2023Quote: ... The disrupted cells were subjected to ultracentrifugation at 4°C and 235000 g for 1h (Beckman Optima™ L-80 XP, rotor Ti45). The resulting supernatant was loaded onto an Äkta purifier system (GE HealthCare ...
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bioRxiv - Cancer Biology 2023Quote: ... the supernatant was ultracentrifuged at 100,000 × g at 4°C for 90 min using Beckman Coulter Optima™ XE-90 Ultracentrifuge (Beckman Colter, Brea, CA) equipped with Type 45 Ti rotor ...
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bioRxiv - Cell Biology 2023Quote: ... the resulting G-actin mix was ultracentrifuged at 90K rpm for 30 minutes at 4°C in an ultracentrifuge (Beckman, Optima TL 100) to remove actin oligomers and/or aggregates.
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bioRxiv - Bioengineering 2023Quote: ... The diluted sample was then centrifuged using a Beckman ultracentrifuge (Optima L90K) with two cycles of 1h10 at 110,000 x g at 4°C (rotor type 70Ti, Beckman Floor Ultracentrifuge Optima L90K). Between them ...
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bioRxiv - Biophysics 2023Quote: ... Virions were banded by density gradient centrifugation at 152,000 x g for 16 h at 4°C in a SW41 rotor (Beckman Coulter SRL, Milan, Italy). The gradient was then fractionated ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... the cell lysate was layered on top of a discontinuous OptiPrep® gradient (50 %, 35 %, 30 %, 20 %, and 15 %) and centrifuged at 135,000 g for 1 h at 4°C (Beckman L8-80M Ultracentrifuge, USA). The melanin pellet was recovered ...
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bioRxiv - Cell Biology 2024Quote: ... and centrifuged at 16,500 × g (14,500 rpm) at 4°C for 20 min using a Type Ti 45 fixed-angle rotor (Beckman Coulter, k-factor 1.275) to isolate large EVs ...
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bioRxiv - Cell Biology 2019Quote: ... The protein solution was then centrifuged at 100,000 rpm in an Airfuge (Beckman Coulter) to remove aggregates ...
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bioRxiv - Microbiology 2019Quote: ... RNA was density separated by centrifugation at 124 000 g at 20 °C for 65 h using an Optima L-90 XP ultracentrifuge (Beckman Coulter, Brea, California, USA). 13 fractions were obtained from each sample ...
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bioRxiv - Cancer Biology 2019Quote: ... 10,000 × g (30 minutes at 4°C) and 100,000 × g (120 minutes at 4°C) by using a Beckman ultracentrifuge (AvantiJ-30I; Beckman Coulter, Inc., Brea, CA, USA). The final sediment was resuspended in the ExoQuick-TC exosome precipitation solution (System Biosciences ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and supernatants were ultracentrifuged at 37 000 rpm (~100 000 g) and 10°C for 1h (Beckman Optima LE-80 K; rotor 70.1.Ti). Pellets were resuspended in 100μL of buffer (10 mM Tris-HCL ...
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bioRxiv - Biophysics 2021Quote: ... Following incubation (37 °C, 20-24 h), the samples were ultracentrifuged (1h, 288’000 x g) (Optima TLX Ultracentrifuge, Beckman Coulter, TLA-120.2 Beckman rotor) to remove large aggregates ...
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bioRxiv - Neuroscience 2019Quote: ... and 1□mM DTT) before a centrifugation process of 2 h 20 min at 30,000□× g (Beckman J-25 centrifuge with a J13.1 rotor). The supernatant was carefully aspirated and the extracted nuclei in a form of a pellet were resuspended in a nuclear storage buffer (15% sucrose ...
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bioRxiv - Microbiology 2020Quote: ... and layered on top of a two-step sucrose gradient (20%/50% w/v in HNE) and centrifuged for 2 h at 154 000 x g at 4 °C in a SW41 rotor (Beckman Coulter, Woerden, the Netherlands) to clear from cell debris ...
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bioRxiv - Neuroscience 2022Quote: ... The samples were then spun at 31000 x g (18468 rpm) for 5 min at 4°C in a fixed-angle rotor (50.2 Ti rotor in Beckman Coulter Optima L-100 XP) with slow brake ...
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bioRxiv - Molecular Biology 2023Quote: ... and were centrifuged at 120,000 g for 120 minutes at 4°C with a MLA55 fixed-angle rotor using an Optima MAX-XP ultracentrifuge (Beckman Coulter, California, United States). The pelleted hVSMCs EVs were resuspended in 100 μL PBS and washed in 13.2 mL PBS by ultracentrifugation at 120,000 g for 60 minutes at 4°C ...
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bioRxiv - Biochemistry 2024Quote: ... Triton X-100 was added to a 0.1% (v/v) and centrifuged for 45 minutes at 20,000 g at 1℃ (Beckman Coulter J2-21, JA 20 rotor). The supernatant was loaded onto a 5 ml HisTrap FF Crude column (Cytiva) ...