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Citations for Beckman :
901 - 950 of 959 citations for 7 16 DIHYDROBENZO A BENZO 5 6 QUINO 3 2 I ACRIDINE 9 18 DIONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... The gradient tubes were subjected to centrifugation at 135,000 × g for 2 h at 4 °C using a fixed-angle rotor (TLA-55, Beckman Coulter, USA). After DGUC ...
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bioRxiv - Bioengineering 2023Quote: ... The gradient tube was balanced and subjected to ultracentrifugation at 150,000 × g for 2 h at 4 °C using a swinging-bucket rotor (SW 41 Ti Beckman Coulter, USA).
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bioRxiv - Biochemistry 2024Quote: ... 50 mM Tris-HCl pH 7.4) and ultracentrifuged at 250 000 x g for 2 h (Beckman Optima TL 100 Ultracentrifuge). After ultracentrifugation ...
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bioRxiv - Genomics 2024Quote: ... The crude nuclei were stained with 4,6-diamidino-2-phenylindole (DAPI) and loaded into a flow cytometer (Beckman Coulter, MoFlo XDP). Nuclei were purified by flow sorting and washed as described previously [61] ...
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bioRxiv - Microbiology 2024Quote: Intracellular HIV-1 p24 staining was performed on fixed U1 and ACH-2 cells with KC57-FITC (Beckman Coulter, Cat#6604665). After allowing at least 30 minutes for fixation ...
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bioRxiv - Neuroscience 2022Quote: ... The samples were then spun at 31000 x g (18468 rpm) for 5 min at 4°C in a fixed-angle rotor (50.2 Ti rotor in Beckman Coulter Optima L-100 XP) with slow brake ...
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bioRxiv - Cell Biology 2023Quote: ... The filters were washed with 5 ml of cold wash buffer and then counted using a liquid scintillation counter (Beckman Coulter LS6500 scintillation counter).
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bioRxiv - Neuroscience 2023Quote: ... PI (1 μl/100 μl) was added 5 minutes before starting the reading on the flow cytometer CytoFlex S (Beckman Coulter, Brea, CA, USA). Results were shown and analyzed through CytExpert flow cytometry analysis software.
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bioRxiv - Immunology 2024Quote: ... Unsupervised clustering of reduced clinical data was performed by FlowSOM (hierarchical consensus clustering; 25 clusters, 5 metaclusters, 10 iterations).81 All analyses were performed using Cytobank (Beckman Coulter Inc., Brea, CA).
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bioRxiv - Immunology 2021Quote: ... The pelleted exosomes from above supernatants were further centrifuged at 100,000g (26,450rpm) for 2 h at 4 °C (Beckman Coulter, Optima XE-90) and resuspended in PBS ...
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bioRxiv - Microbiology 2021Quote: ... Samples were centrifuged at 130,000 × g for 2 h at 4°C in a TLA100.3 rotor (Beckman Optima TLX table top centrifuge). Following centrifugation ...
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bioRxiv - Neuroscience 2021Quote: ... The post nuclear supernatant was transferred to an ultracentrifuge tube and centrifuged for 2 h at 245,000 g at 4 °C in a Beckman L90 Ultracentrifuge (Beckman Coulter, Mississauga, Canada) to pellet remaining membrane.
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bioRxiv - Neuroscience 2020Quote: ... Final 15 mL of solution was mixed by inverting the tube 10x and carefully pipetted into 2 ultracentrifuge tubes (Beckman Coulter #344059) onto the isolation buffer cushion (5 mL ...
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bioRxiv - Biochemistry 2020Quote: ... to a final volume of 1 ml and then further centrifuged (120000 g, 4°C, 2 h) in a TLA-120.2 rotor (Beckman Coulter, Brea, CA). Pellets were washed twice with 4°C cold PBS and centrifuged (120000 g ...
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bioRxiv - Microbiology 2020Quote: ... were pelleted through a 20% (wt/vol) sucrose cushion at 25,000 rpm for 2 h in an SW41 rotor (Beckman Coulter, Tokyo, Japan). Then ...
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Micro RNAs are minor constituents of extracellular vesicles and are hardly delivered to target cellsbioRxiv - Cell Biology 2020Quote: ... Cat no UZ-PA-38.5-1) at 100,000 g at 4 °C for 2 h in a swinging-bucket rotor (Beckman Coulter; SW28 or SW32).
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bioRxiv - Neuroscience 2022Quote: ... which was then sealed and placed into a hematocrit spinner (2 minutes at 13, 700 g; StatSpin CritSpin Microhematocrit Centrifuge, Beckman Coulter, Inc). Measurements were taken to calculate percent (% ...
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bioRxiv - Cancer Biology 2022Quote: ... The cell pellet was disaggregated and incubated with 2 μL of anti-pan cytokeratin-FITC (Beckman Coulter, Cat. No. IM2356U, Clone J1B3). A separate tube containing cells unstained for surface E-Cadherin was incubated after permeabilization with E-Cadherin antibody- (R&D Cat ...
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SLC15A4 favors inflammasome function via mTORC1 signaling and autophagy restraint in dendritic cellsbioRxiv - Immunology 2022Quote: ... Cells were finally washed in PBS with 0.5% BSA and 2 mM EDTA and analyzed by flow cytometry using a CytoFLEX (Beckman Coulter, Indianapolis, IN) and FlowJo software (BD Biosciences) ...
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bioRxiv - Immunology 2022Quote: ... Ultracentrifugation in a Beckman L7-65 ultracentrifuge at 25,000 rpm for 2 hours at 4°C using a Beckman SW28 rotor (Beckman Coulter, CA, USA) was performed to pellet the viruses through the sucrose cushion while soluble egg proteins were removed ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Clarified supernatants were layered over 2 ml of a 25% (w/v) sucrose solution in 13.2 ml Thinwall Polypropylene SW41 Ti ultracentrifuge tubes (Beckman-Coulter, Brea, USA) and made up to a total volume of 10 ml with PBS without calcium chloride or magnesium chloride (PBS[-]) ...
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bioRxiv - Microbiology 2019Quote: ... and viruses were pelleted via ultracentrifugation for 2 hours at 125,000 x g at 4°C using an SW55-Ti rotor (Beckman Coulter, Indianapolis, IN). Following centrifugation ...
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bioRxiv - Molecular Biology 2020Quote: ... was subjected to centrifugation at 100,000g for 2 h in an Optima MAX-XP benchtop ultracentrifuge with a TLA-100.3 rotor (Beckman Coulter, Inc., Brea, CA). Pellets were resuspended in 100 µl PBS and stored at −80 °C.
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bioRxiv - Neuroscience 2020Quote: ... Homogenates were layered on top of 1 ml of 0.85 M sucrose in 2 mM EGTA and centrifuged at 75,000 g for 30 min in a Beckman FW-27 rotor (Beckman, Fullerton, CA, USA). This step was repeat twice ...
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bioRxiv - Microbiology 2021Quote: ... soil was thoroughly mixed with 20 ml of 0.01 M CaCl2 for 30 min on a Elmi Intelli-Mixer RM-2 (Elmi Tech Ltd, Latvia) and subjected to centrifugation in an Allegra X-22R centrifuge (Beckman Coulter, USA) at 3900 rcf for 20 min and to remove inorganic S (Dedourge et al. ...
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bioRxiv - Microbiology 2023Quote: ... the prepared DNA library was size-selected on a 2% agarose gel (410–470 bp) using the Agencourt SPRI system to reduce mitochondrial contamination (Beckman Coulter, Inc.). After library prep ...
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bioRxiv - Cell Biology 2023Quote: ... cells were blocked in 2% human serum and PBS for 30 min at 4°C and stained with DC-SIGN (Beckman Coulter #A07407), CD80 (BioLegend #305214) ...
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bioRxiv - Plant Biology 2023Quote: ... 700 μl of obtained extract was applied to a chilled sucrose density gradient and centrifuged at 4 °C for 2 hours 45 minutes at 151,000 ×g using the SW 41 Ti rotor (Beckman Culture Life Sciences). After centrifugation ...
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bioRxiv - Molecular Biology 2023Quote: ... The lysates were loaded onto the sucrose gradients and centrifuged at 40’000 rpm for 2 h at 4°C using the SW 41 Ti rotor (Beckman Coulter, Cat# 331362). Subsequently ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were centrifuged at 100,000 × g for 2 hr at 4 °C (Type 50.2 Ti Fixed angle ultracentrifuge rotor, Beckman Coulter, Brea, CA, USA). The supernatants were aspirated ...
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bioRxiv - Plant Biology 2023Quote: ... DNA extracts were purified using a bead clean up method with a 2:1 ratio of Ampure XP beads (Beckman Coulter, USA) to DNA elution ...
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bioRxiv - Biophysics 2023Quote: ... The virions were concentrated by ultracentrifugation through a 20% (w/v) sucrose cushion (2 h at 28,000 rpm in a SW32 rotor [Beckman Coulter Life Sciences]). The pellet was resuspended in PBS ...
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bioRxiv - Genetics 2023Quote: ... in-house VLPs were produced using the same protocol followed by ultracentrifugation at 24,000 RPM for 2 hours (SW 28 Beckman swinging bucket rotor) to concentrate the VLPs and avoid diluting the HSPCs culture media ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were centrifuged at 60,000 rpm at 4°C for 2 h on an SW65 Ti ultracentrifuge rotor (Beckman Coulter, California, USA). The pellets were gently washed by dispensing and removing 500 µl of ice-cold RNase-free water ...
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bioRxiv - Biophysics 2021Quote: ... The top AAF-modified oligonucleotides were separated from unmodified ones by C18-reversed phase high-performance liquid chromatography (Beckman Ultrasphere 5 μm; φ4.6 × 250 mm). The column was equilibrated sample loaded with a mixture of 100 mM triethylamine acetate (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... 2.5 ml of each solution were used to form the gradient in a 14 ml Ultra-Clear™ centrifuge tube (Beckman Coulter, Brea, CA, USA), with the highest sucrose concentration at the bottom of the tube ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Blood samples were taken from animals and analyzed to get complete blood count (in terms of RBSs, WBCs, platelets, and hemoglobin) using automated electronic counter (Hematology auto-analyzer - BECKMAN COULTER, ACT. 5 diff. USA).
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bioRxiv - Cancer Biology 2020Quote: ... and NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 1 E7335S, Index Primers Set 2, E7500S) with Agencourt AMPure XP magnetic beads (Beckman coulter A63881) exactly as per manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... and extracellular vesicles including exosomes and virions were pelleted by ultracentrifugation at 141,000 g at 4 °C for 2 h in a swinging-bucket rotor (SW28; Beckman Coulter, Brea, CA, USA). The pellet was resuspended in filtered PBS and centrifuged at 18,300 g and 4°C for 10 min to remove insoluble aggregates ...
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bioRxiv - Microbiology 2020Quote: ... The clarified supernatant was overlaid onto a 20% (w/v) sucrose cushion in Tris-NaCl-EDTA buffer and centrifuged at 134,000 g for 2 hours (Beckman Coulter 30 Ti rotor). The resulting viral pellet was suspended in VCM by repeat pipetting and aliquots stored in cryovials at minus 70°C until needed ...
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bioRxiv - Genomics 2020Quote: ... were layered onto a 1.8M sucrose cushion and ultra-centrifuged at 24,400 rpm at 4°C for 2 hours using a SW28 swinging bucket rotor (Beckman Coulter Optimal centrifuge #L90K). The nuclear pellet was re-suspended in 2% BSA in 1X PBS ...
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bioRxiv - Microbiology 2020Quote: ... EMVs were obtained by ultracentrifugation from 4 mL of filtrate at 100,000 × g (average centrifugal force) and 4°C for 2 h with an OptimaX centrifuge (Beckman Coulter, Brea, CA, USA). The pellets were resuspended in 400 μL of Dulbecco’s phosphate-buffered saline (DPBS ...
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bioRxiv - Neuroscience 2022Quote: ... The tubes were sealed and centrifuged at 50,000 rpm at 4°C for 2 h in an OptimaTM L-90K ultracentrifuge using the 70.1Ti rotor (Beckman Coulter, Brea, CA, USA). After the centrifugation ...
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bioRxiv - Cell Biology 2020Quote: ... 3 μM or 10 μM from a 2 mM or 10 mM stock solution using a Biomek FX with 384-well head (Beckman Coulter, Indianapolis, IN). The DMSO concentration was kept below or at 0.5% v/v ...
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bioRxiv - Physiology 2022Quote: ... the library preparation step followed the CEL-Seq 2 protocol85: the RT product was digested by ExoI and HinFI and purified using AMpure XP beads (Beckman Coulter™, USA). Then ...
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bioRxiv - Microbiology 2023Quote: ... then the colonies were diluted and inoculated into the Neg MIC EN-2J panel containing β-lactams listed in Table 2 and incubated in MicroScan Walkway40 Plus (Beckman Coulter Inc.). Breakpoint criteria for susceptible (S) ...
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bioRxiv - Neuroscience 2020Quote: ... for further depletion of cell debris and spun at 10,000 × g for 30 min at 4°C (AH-650 rotor, Beckman Ultra-Clear Tube with 5 ml capacity). The pellet was resuspended in 100 μl PBS and considered to be the “10K pellet” (10K or 10K fraction) ...
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bioRxiv - Molecular Biology 2020Quote: ... bound antibodies were visualized using 100 µL of a 1:100 dilution of goat F(ab’)2 anti-human IgG coupled to phycoerythrin (Beckman Coulter, Brea, CA, USA) at 4°C for 30 min ...
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bioRxiv - Biochemistry 2020Quote: Sedimentation velocity experiments were performed using a ProteomeLab XL-1 analytical ultracentrifuge with the use of an AN60-Ti rotor and Epon-2 channel centerpieces (Beckman Coulter, Brea, CA, USA). Protein samples were diluted with SANS buffer to concentrations yielding an absorbance of 0.5 at 280 nm as measured with an 8454 UV–Vis spectrophotometer (Agilent ...
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bioRxiv - Microbiology 2022Quote: ... The success of the amplifications was checked in a 2% agarose gel and PCR products were subsequently purified using Agencourt AMPure XP magnetic beads kit (Beckman Coulter, Brea, CA, USA), quantified with the QuantiFluor™ dsDNA System (Promega ...