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Citations for Beckman :
851 - 900 of 1527 citations for G Protein Signaling Modulator 1 GPSM1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... supernatants were centrifuged at 10,000 x g for 30 min at 4°C and the supernatants were transferred to ultracentrifuge tubes (Beckman Coulter, Brea, CA) and diluted with phosphate-buffered saline (PBS) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Mitochondria were purified using a series of differential centrifugations: 20 min at 1500 g to pellet residual chloroplasts (8 °C, 3500 RPM, Beckman JA-12), 20 min at 20 000 g to pellet mitochondria (8 °C ...
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bioRxiv - Neuroscience 2020Quote: ... Homogenates were layered on top of 1 ml of 0.85 M sucrose in 2 mM EGTA and centrifuged at 75,000 g for 30 min in a Beckman FW-27 rotor (Beckman, Fullerton, CA, USA). This step was repeat twice ...
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bioRxiv - Microbiology 2021Quote: ... density gradient (35 to 0% in PBS) and ultracentrifuged at 200,000 × g for at least 12 hours at 4 °C (Beckman Optima XL-100K). The corresponding clear yellow band was collected ...
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bioRxiv - Cell Biology 2022Quote: ... The resulting supernatant was centrifuged at 10,000 x g for 30 minutes to remove any remaining debris and then ultra-centrifuged at 118,000 x g for 70 minutes at 4°C with a fixed-angle rotor (Ti-70, Beckman Coulter, Brea, CA). The pellet was washed with sterile 1X PBS and subjected to another cycle of ultracentrifugation at 118,000 x g for 70 minutes at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... The supernatants were further centrifuged at 10,000 × g for 30 min at 4°C to remove remaining cell debris and at 100,000 × g (Type 90 Ti Rotor, Beckman, Brea, CA, USA) overnight at 4°C to remove exosomes ...
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bioRxiv - Microbiology 2022Quote: ... Supernatant was collected and virus pelleted through 30% (w/v) sucrose cushion at 151,000 × g (using a Beckman SW 32 Ti rotor) for 3.5 hours at 10°C ...
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bioRxiv - Microbiology 2022Quote: ... The supernatant was collected and purified through 15-45% (w/v) sucrose density gradient by ultracentrifugation at 151,000 × g (using a 17 mL Beckman SW32.1 Ti rotor) for 3 hours at 10°C (18) ...
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bioRxiv - Microbiology 2022Quote: ... Supernatant was purified through 15-45% (w/v) sucrose density gradient by ultracentrifugation at 151,000 × g (using a Beckman SW 40 rotor) for 3 hours at 10°C (18).
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Isolation and characterization of a novel phage of Vibrio parahaemolyticus with biocontrol potentialbioRxiv - Microbiology 2022Quote: ... for purification and centrifuged at 200,000 × g at 4°C for 24 h using an Optima L-100 XP ultracentrifuge (Beckman Coulter, CA, USA). The purified phage particles were dialyzed through 30-kDa superfilters (Millipore ...
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bioRxiv - Neuroscience 2023Quote: ... re-suspend in the homogenization buffer and centrifuged at 100,000 x g for 40 min at 4°C (Beckman SWTi41 swing rotor). The pellet containing the growth cone were re-suspended in 50 μl of the homogenization buffer ...
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bioRxiv - Microbiology 2022Quote: ... Supernatant was purified through 15-45% (w/v) sucrose density gradient by ultracentrifugation at 151,000 × g (using a 17 mL Beckman SW32.1 Ti rotor) for 3 hours at 10°C (16) ...
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bioRxiv - Biochemistry 2022Quote: ... The supernatant was again centrifuged at 100000 g for 45 min at 4°C (Beckman Optima™ MAX-XP Rotor MLS-50). After filtering the supernatant through a 0.22 μm syringe filter ...
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bioRxiv - Cell Biology 2022Quote: ... One milliliter of concentrated conditioned medium was pipetted on top of the gradient and samples were centrifuged for 18 h at 100,000 ×g and 4 °C using a SW 32.1 Ti rotor (Beckman Coulter, Fullerton, California, USA). Fractions of 1 mL were collected from the top and EV-rich fractions 9 and 10 (corresponding to a density of 1.10-1.12 g/mL ...
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bioRxiv - Neuroscience 2023Quote: ... then spun at 100,000 x g for 30 min at 4°C in an ultracentrifuge (Beckman OPTIMA TLX with TLA-100.3 rotor). The supernatant was saved and the pellet went through a series of similar extractions using High-Salt Triton Buffer (High-Salt Buffer ...
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bioRxiv - Biochemistry 2022Quote: ... VLPs were concentrated over 500 μL of 20% sucrose in PBS at 50,000 x g for 45 min (Beckman Coulter Optima TLX). Infections were assessed by western blot using the following antibodies ...
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bioRxiv - Cell Biology 2023Quote: ... was next subjected to ultracentrifugation at 120,000 × g for 4 h in a SW 28 Ti Swinging-Bucket Rotor (Beckman Coulter, Fullerton, CA) to sediment small EVs ...
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bioRxiv - Plant Biology 2023Quote: ... 700 μl of obtained extract was applied to a chilled sucrose density gradient and centrifuged at 4 °C for 2 hours 45 minutes at 151,000 ×g using the SW 41 Ti rotor (Beckman Culture Life Sciences). After centrifugation ...
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bioRxiv - Cell Biology 2023Quote: ... EV–containing fractions were pooled (1.10–1.18 g/mL) and diluted by adding 9 ml PBS to SW40 tubes (Beckman–Coulter, Fullerton, CA, USA). EVs were pelleted at 200,000xg for 60 min at 4°C (Beckman–Coulter Optima™ L–90K or Optima™ XPN–80 centrifuge ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were centrifuged at 100,000 × g for 2 hr at 4 °C (Type 50.2 Ti Fixed angle ultracentrifuge rotor, Beckman Coulter, Brea, CA, USA). The supernatants were aspirated ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were incubated at RT for 30 minutes and centrifugated at 20,000 x g for 10 minutes (Avanti J-30I Centrifuge, Beckman Coulter, Brea, CA). The supernatant was collected and diluted 1:5 with incubation buffer (final concentration ∼204 cells mL-1) ...
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bioRxiv - Immunology 2023Quote: ... Bacteria cultures were collected and discarded by centrifugation for 60 min at 4,000-8,000 x g and the supernatants were subjected to high-speed centrifugation at 11,9000 x g for 2-3 h at 4°C (Beckman Coulter Optima Ultracentrifuge). The pellets containing the OMVs were washed with PBS ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were centrifuged at 250000 g for 60 minutes in a swinging bucket rotor of the ultracentrifuge (Beckman Coulter Optima L-60). Then ...
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bioRxiv - Biochemistry 2023Quote: ... by centrifugation at 80,000 × g for 30 min at 4°C using a TLS-55 rotor (Beckman Coulter Inc., Miami, FL, USA). Crista membranes were identified and removed based on fluorescence under a UV lamp ...
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bioRxiv - Cell Biology 2023Quote: ... Following centrifugation at 131,300 × g for 24 hr at 4 °C using a MLS-50 Swinging-Bucket rotor (Beckman Coulter, Brea, CA) to sediment protein complexes ...
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bioRxiv - Cancer Biology 2024Quote: ... The plates were then centrifuged at 2000 rpm (931 x g) for 2h at 30°C (Allegra® X-15R Centrifuge (Beckman Coulter), rotor SX4750A) ...
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bioRxiv - Microbiology 2024Quote: ... Saliva was collected by expectoration into tubes on ice followed by centrifugation for 10 minutes at 4°C at 4000 x g (Beckman SX4750 rotor) to remove large debris and bacterial aggregates ...
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bioRxiv - Biophysics 2024Quote: ... The gradients were then centrifuged at 187,000 × g for 4 h at 4°C in a SW 41 Ti rotor (Beckman Coulter, Brea, CA). Bands corresponding to purified virus particles (∼1.36 g/mL ...
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bioRxiv - Cell Biology 2024Quote: ... while the supernatant was further centrifuged at 118.000 × g (38,800 rpm) (Type Ti 45 fixed-angle rotor, Beckman Coulter, k-factor 178) for 2.5 hours at 4°C to isolate small EVs that were resuspended in PBS and stored at –80°C.
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bioRxiv - Cell Biology 2024Quote: ... and cells were isolated by centrifugation at 500 g for 5 min at 4°C followed by being treated with a red blood lysis buffer (Beckman Coulter, USA). After washing with PBS ...
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bioRxiv - Biochemistry 2023Quote: ... Cultures were then recovered by centrifugation (4,000 x g, 4 °C, 40 min in a Beckman Avanti JXN-26 (rotor: JLA 8.1)) and pellets resuspended in approximatively 30 mL of lysis buffer (20 mM HEPES ...
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bioRxiv - Molecular Biology 2024Quote: ... ADEV isolation was performed by ultracentrifugation at 100,000 × g for 14 h at 4 °C (swing bucket rotor on minimum acceleration/break setting, SW 32Ti Beckman Coulter, Brea, CA), and ADEVs were resuspended in 200μL PBS and stored at −20°C until used16,41 ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein complexes were then fractionated by centrifugation in an SW 41Ti rotor (Beckman) at 38,000 rpm ...
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bioRxiv - Biophysics 2021Quote: ... 1ml acceptor LUV was diluted with 10 mM tris 100 mM NaCl and centrifuged to remove CsCl or sucrose (188,965 g, 37,500 rpm, 45 min, Beckman L8-80M, SW-60 rotor). The acceptor LUV pellet was resuspended in 1ml 20 mM tris 100 mM NaCl pH 7.4 buffer ...
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bioRxiv - Cell Biology 2020Quote: ... and the final supernatant was centrifuged at 100,000 × g for 90 min in an SWT32 swinging bucket rotor (Beckman Coulter, Brea, CA, USA) at 4 °C to precipitate the EVs ...
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bioRxiv - Molecular Biology 2021Quote: ... The centrifugation is performed on a SW60Ti rotor at 40 000 g for 22 minutes at 4°C (Beckman Optima L-90K Ultracentrifuge). The mitochondrial fraction (400µL ...
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bioRxiv - Molecular Biology 2020Quote: ... and plasma was separated by centrifugation (2,000 × g, 20 min, 4 °C) in a Microfuge 20R (cat. no. B30149, Beckman Coulter, Brea, CA, USA). Plasma was transferred to new 1.5 mL microcentrifuge tubes and further clarified by centrifugation (10,000 × g ...
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bioRxiv - Biophysics 2022Quote: ... shortly sonified and centrifuged for 30 min at 100 000 g at 4°C (Centrifuge Beckman Coulter Optima XPN-80, Rotor type 70.1).) ...
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bioRxiv - Biophysics 2022Quote: ... The samples were centrifuged for 30 min at 100 000 g at 4°C (Centrifuge Beckman Coulter Optima XPN-80, Rotor type 70.1). The supernatant ...
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bioRxiv - Microbiology 2020Quote: ... CLP assembly reactions were incubated at 25°C for 30 min before loading onto 15-30% (wt/wt) sucrose gradients and spinning at 159,599 X g for 43 min at 4°C (Beckman Coulter TLS 55 rotor). Gradients were immediately fractionated and aliquots of each fraction were denatured by boiling in 1% SDS to ensure antibody access ...
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bioRxiv - Neuroscience 2019Quote: ... The homogenates were then centrifuged for 15 minutes at 1500 × g (5000 rpm) in a rotor (Beckman Coulter Ti50, USA, CA, Palo Alto). The supernatant was analyzed using a Fish Glutamine Synthetase ELISA Kit (MBS ...
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bioRxiv - Bioengineering 2020Quote: ... and the supernatant was centrifuged again at 26000 × g at 4 °C for 15 min using an Optima L-90K (Beckman Coulter, Brea, CA) ultracentrifuge with a 70-Ti fixed angle rotor (Beckman Coulter) ...
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bioRxiv - Biochemistry 2021Quote: ... supernatants were collected and centrifuged with 150.000 x g (at rav) for 30 min at 4 °C using an Optima™ MAX-XP Ultracentrifuge (Beckman Coulter; TLA120.2 rotor). Pellets were resuspended in PBS and 4 µg of primary antibody were added for 30 min ...
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bioRxiv - Biochemistry 2020Quote: ... was then added to the product which passed microfluidizer and unbroken cells and large debris were eliminated by centrifugation at 4000 × g (Beckman Coulter™, USA). The supernatant containing the IMVs was collected and washed several times with resuspension buffer by centrifugation at approximately 310,000 × g at 4°C for 30 minutes (SORVALL® Centrifuges ...
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bioRxiv - Biophysics 2020Quote: ... 150 μl of the sample was ultracentrifuged at 22°C and 205000 × g for 19 min (TLA 100.3, Beckman Coulter Inc., Fullerton, California). The experiments were performed in HEPES buffer ...
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bioRxiv - Bioengineering 2021Quote: ... The samples were then centrifuged at 12,000 g for five minutes at 4°C to remove tissue debris with a Microfuge™ 22R centrifuge (Beckman Coulter, Brea, CA). The RNA was extracted using the Invitrogen PureLink™ RNA Mini Kit (Thermo Fisher ...
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bioRxiv - Bioengineering 2021Quote: ... The tubes were then centrifuged at 1000 g for 15 minutes at 4°C using a Microfuge™ 22R (Beckman Coulter, Brea, CA), and the serum was then stored at -80°C ...
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bioRxiv - Microbiology 2021Quote: ... and extracellular vesicles including exosomes and virions were pelleted by ultracentrifugation at 141,000 g at 4 °C for 2 h in a swinging-bucket rotor (SW28; Beckman Coulter, Brea, CA, USA). The pellet was resuspended in filtered PBS and centrifuged at 18,300 g and 4°C for 10 min to remove insoluble aggregates ...
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bioRxiv - Microbiology 2021Quote: ... resuspended in 150 μl 0.1 M HEPES/NAOH pH 7.0 and purified for 48 h (250,000 × g, 4 °C; Beckman Optima LE-80K, rotor SW60-Ti) using a CsCl gradient (0.45 g/ml ...
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bioRxiv - Microbiology 2019Quote: ... and the remaining 32 mL were centrifuged at 500 × g for 3 min at 4°C (Allegra X-15R, Beckman Coulter, Villepinte, France) to spin down the remaining meat fibers and debris ...