Labshake search
Citations for Beckman :
851 - 900 of 1643 citations for 7H Cyclopenta c pyridin 7 one 5 6 dihydro 3 methyl 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... Then the lysates were spin down at 67,000 rpm for 1 hour at 18 °C with the ultracentrifuge (Beckman). The AAV particles were collected by collecting the interface between 40 and 60% iodixanol with a syringe ...
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bioRxiv - Biochemistry 2023Quote: ... lysates were centrifuged at 60,000 x g (4 °C) for 45 min (Beckman Avanti JXN-26 (rotor: JA 25.5)) and immediately supplemented with 10 mL pre-washed Ni2+-NTA agarose (QIAGEN ...
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bioRxiv - Cancer Biology 2024Quote: ... 100 μg/mL cycloheximide) for 2 h at 40,000 g at 4 °C using an SW41 rotor (Beckman Coulter). Gradients were fractionated using Teledyne Isco Tris Peristaltic Pump and fractions were collected and pooled according to the UV trace ...
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bioRxiv - Molecular Biology 2024Quote: ... for ultracentrifugation at 100,000 × g for 1 hour at 4°C (JXN-30 centrifuge, JA-30.50 Ti Rotor, Beckman Coulter). The pellet was washed with PBS and centrifuged again at 100,000 × g for 1 hour at 4°C to remove soluble contaminant ...
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bioRxiv - Neuroscience 2024Quote: ... by centrifugation at 5,000 x g at 4°C for 30 minutes in a fixed angle rotor (Beckman Coulter). The qEV column washed with 1x PBS was then loaded with 500 µL of concentrated supernatant followed by 2.5 mL PBS to collect 3 mL void volume ...
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bioRxiv - Neuroscience 2024Quote: ... and PBS was added to reach a 4.5 mL tube filling and centrifuged at 120,000xg for 100 min at 4°C in the L-60 Optima Ultracentrifuge (Beckman Coulter ...
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bioRxiv - Neuroscience 2024Quote: ... Ultracentrifugation was performed for 2.5 h at 18 °C at 350,000 × g in a type 70 Ti rotor (Beckman Coulter). The interface between the 60 and 40% gradient was extracted along with the 40% layer ...
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bioRxiv - Cell Biology 2024Quote: ... samples were ultracentrifuged at 160,000 xg for 2 h at 4 °C in ultracentrifuge tubes (Beckman Coulter Cat #328874). The resulting pellet contained the insoluble AMB-1 cell fraction and the supernatant contained the soluble fraction ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were spun at 200,000 × g at 4°C for 4 h in an MLS-50 rotor (Beckman Coulter), and 60 µL of top layer was collected ...
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bioRxiv - Cell Biology 2024Quote: ... The supernatant was then subjected to ultracentrifugation at 100,000 x g for 2 h at 4 °C to sediment small EVs (Optima L-80K ultracentrifuge, Beckman Coulter ...
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bioRxiv - Molecular Biology 2024Quote: ... and 1 M sucrose) at 100,000 rpm (543,000 × g) and 4°C for 1 h with a TLA110 rotor (Beckman Coulter) and an Optima MAX-TL Ultracentrifuge (Beckman Coulter) ...
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bioRxiv - Developmental Biology 2023Quote: ... the supernatant was centrifuged in a TLA100.4 fixed angle rotor at 100000 g for 1h at 37°C in an Optima TL ultracentrifuge (Beckman). The supernatant containing globular actin was collected ...
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bioRxiv - Genetics 2023Quote: ... Fifteen A260 units of WCEs were layered on a pre-chilled 10-50% sucrose gradient and centrifuged at 40,000 rpm for 2 h at 4°C in a SW41Ti rotor (Beckman). Gradient fractions were continuously scanned at A260 using the BioComp Gradient Station and polysome to monosome (P/M ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were ultracentrifuged at 100,000 g for 30 minutes at 4°C in a Optima XPN-100 ultracentrifuge (Beckman). Supernatants were kept aside as “Supernatant” fraction ...
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bioRxiv - Microbiology 2023Quote: ... All centrifugation steps were performed at 4 °C in an Avanti J-E high-speed fixed-angle centrifuge (Beckman Coulter Life Sciences ...
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bioRxiv - Neuroscience 2023Quote: ... DTT-treated EVs were pelleted at 100,000 x g for 70 min at 4°C in a TLA100 rotor (Beckman). The supernatant ...
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bioRxiv - Neuroscience 2023Quote: ... The prepared gradients were centrifuged at 200,000 x g for 16 h at 4°C in an SW41Ti rotor (Beckman), with no braking during deceleration ...
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bioRxiv - Neuroscience 2023Quote: ... The pellet was resuspended in 4.5 volumes of ice-cold extraction buffer and re- centrifuged at 11,300 RPM for 10 min at 4°C using a Ti45 rotor (Beckman). The supernatant was filtered through a 70 μm pore size cell-strainer and combined with the first supernatant ...
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bioRxiv - Neuroscience 2023Quote: ... The tube was topped up to 23 mL with PBS and centrifuged at 59,300 RPM for 30 min at 4°C using a Ti70 rotor (Beckman). The floating lipid layer and supernatant were discarded and the pellet was transferred to a 2 mL tube in 50 μL PBS per g initial grey matter ...
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bioRxiv - Neuroscience 2023Quote: ... The homogenate was centrifuged at 11,300 revolutions per minute (RPM) for 10 min at 4°C using a Ti45 rotor (Beckman). The supernatant was filtered through a 70 μm pore size cell-strainer and retained on ice ...
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bioRxiv - Neuroscience 2023Quote: ... The supernatant was transferred to fresh tubes and centrifuged at 100,000 x g for 70 min at 4°C using a Ti45 rotor (Beckman). The 100,000 x g pellets were resuspended in 1 mL ice-cold PBS and brought to 60 mL using PBS ...
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bioRxiv - Microbiology 2023Quote: ... The lysates containing matured PsVs were loaded on an OptiPrep gradient that had been stabilized at least 1 h and centrifuged at 50k x g for 3.5-4 h at 4°C in a SW-55Ti rotor (Beckman). Fractions were collected in siliconized tubes and subjected to SDS-PAGE followed by Coomassie blue staining to assess the abundance of L1 and L2 proteins ...
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bioRxiv - Microbiology 2022Quote: ... Cell debris was removed by centrifugation at 14,800 rpm for 20 minutes 4 °C in F241.5P rotor using 149 Microfuge 22R centrifuge (Beckman Coulter). The supernatant was combined with 100 µL of ANTI-FLAG M2 Affinity Gel (Sigma ...
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bioRxiv - Neuroscience 2023Quote: ... The lysate was centrifuged at 40,000 rpm for 50 min at 4°C (45 Ti fixed-angle rotor, Beckman) and the transporter-Fab complex was isolated by affinity chromatography using Strep-Tactin resin ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were lysed by extrusion using a Teflon Dounce homogenizer and clarified by centrifugation at 30000g for 40 minutes at 4°C using a Ja 25.50 rotor (Beckman). The clarified lysate was incubated with 2 ml of pre-equilibrated Protein C affinity resin (Roche ...
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bioRxiv - Biophysics 2023Quote: ... and the resulting lysate was cleared by centrifugation at 279,288 × g for 10 minutes at 4°C using a tabletop centrifuge unit from Beckman Coulter ...
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bioRxiv - Genomics 2023Quote: ... followed by centrifugation at 31,900 ×g (10 min, 4 °C, JA-20 rotor, Avanti J-26 XP, Beckman Coulter). Next ...
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bioRxiv - Biochemistry 2023Quote: ... binding reactions were centrifuged at 50,000 RPM for 30 min at 25 °C in a TLA-100 rotor (Beckman). Promptly after centrifugation ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were then centrifuged at 82,600×g at 4°C for 2 h (Beckman ultracentrifuge optima L-100 XP). After the first ultracentrifugation ...
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An adaptive biomolecular condensation response is conserved across environmentally divergent speciesbioRxiv - Cell Biology 2023Quote: ... 500 µL was transferred to a vacuum safe tube spun at 100,000 g for 20 minutes at 4°C (fixed-angle TLA-55 rotor in a Beckman Coulter Optimax tabletop ultracentrifuge) ...
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bioRxiv - Plant Biology 2023Quote: ... Approximately 250 µl of crude EV sample resuspended in 20 mM Tris-HCl pH 6.0 was slowly dispensed on top of the column and centrifuged at 100,000 x g for 17 h at 4 ⁰C (SW 41 Ti rotor, Beckman Coulter ...
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bioRxiv - Biochemistry 2023Quote: ... at 40% on a Branson Digital Sonifier and clarified by centrifugation for 30 minutes at 27,216 × g at 4°C in an SS-34 rotor (Avanti J-25 centrifuge, Beckman).
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bioRxiv - Plant Biology 2023Quote: ... The supernatant from this step was decanted into the new tubes and centrifuged at 39,800 x g for 2 h at 4 ⁰C (JA-17 rotor, Beckman Coulter ...
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bioRxiv - Biophysics 2023Quote: ... The cell suspension was boiled for 20 min at 85 °C and centrifuged at 18,000 rpm with a JA-25.5 rotor (Beckman Coulter). Streptomycin sulfate was added to the supernatant to a final concentration of 10 mg/mL and the mixture was stirred for 15 min at 4 °C ...
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bioRxiv - Biophysics 2023Quote: ... placed in an An50Ti rotor and centrifugated 50,000 rpm at 4 °C using an Optima XL-I analytical ultracentrifuge (Beckman). The data were analysed in SEDFIT-16.1c 53 using a c(s ...
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bioRxiv - Cell Biology 2023Quote: ... and 1 mM PMSF) and centrifuged for 15 hours at 4°C with 74,400 xg (SW 32.1 Ti; Beckman Coulter). 24 equal volume fractions were collected from the top and subjected to TCA precipitation ...
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bioRxiv - Cancer Biology 2023Quote: ... and centrifuged (33.500 rpm/4°C/3h, brake off) in a Optima XPN-80 ultracentrifuge (Beckman Coulter, Krefeld, Germany). Sucrose gradients were eluted into a BR-188 density gradient fractionation system (Brandel Inc ...
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bioRxiv - Molecular Biology 2023Quote: ... 1-1.5 μg of total protein was loaded onto a 10% to 50% sucrose gradient and centrifuged at 36,000 rpm for 2 hours at 4°C using an SW41 rotor (Beckman). Polysome profiles were visualized ...
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bioRxiv - Plant Biology 2023Quote: ... It was then centrifuged again at 100,000 x g for 1 h at 4 ⁰C (TLA 55 rotor, Beckman Coulter ...
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bioRxiv - Biophysics 2023Quote: ... The supernatant was subjected to a second round of ultracentrifugation at 100,000 x g for 1 hour at 4°C in a Type 45Ti rotor (Beckman) to pellet cell membranes ...
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bioRxiv - Cell Biology 2023Quote: ... was collected before ultracentrifugation at 100,000 g for 30 min at 23°C in a TLA100.3 rotors (Beckman & Coutler). Supernatant was discarded and pellet fractions were obtained by resuspending pellet in Laemmli buffer 1X with volume equal to the initial volume ...
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bioRxiv - Cell Biology 2023Quote: ... was collected before ultracentrifugation at 100,000 g for 30 min at 23°C in a TLA100.3 rotors (Beckman & Coutler). Supernatant was discarded and pellet fractions were obtained by resuspending pellet in Laemmli buffer 1X with volume equal to the initial volume ...
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bioRxiv - Cell Biology 2023Quote: ... 20µM ATP and 1 mM DTT and subsequently sedimented (100,000 x g, 15 min, 4°C, TLA-100 rotor) in an Ultima MAX-XP ultracentrifuge (Beckman). For competition assays ...
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bioRxiv - Plant Biology 2023Quote: ... The tubes were then centrifuged at 100,000 x g for 1 h at 4 ⁰C (TLA 55 rotor, Beckman Coulter ...
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bioRxiv - Biochemistry 2023Quote: ... The lysate was clarified by centrifugation (183,960 x g, 88 min, 4°C) in a Type 70Ti rotor (Beckman). The supernatant was mixed with 2 mL of IgG Sepharose 6 Fast Flow beads (Cytiva ...
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bioRxiv - Biochemistry 2023Quote: ... The lysate was clarified via centrifugation (66,000 x g, 30 min, 4°C) in a Type 70 Ti rotor (Beckman). The supernatant was mixed with 1.5 mL of anti-FLAG M2 affinity gel (Sigma-Aldrich ...
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bioRxiv - Immunology 2022Quote: ... then samples were centrifuged at 2,700 RPM for 10 minutes at 4°C in a tabletop centrifuge (Beckman Coulter). The supernatant (serum ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatant was centrifuged at 200,000 x g for 2.5 h (high g) at 4°C in a TLA-100.3 fixed angle rotor (k-factor 14) (Beckman Coulter) and the pellet was resuspended in 25 ∝l lysis buffer ...
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bioRxiv - Cell Biology 2023Quote: ... Gradients were spun at 45,000 RPM for 16 hours at 4°C in SW55Ti rotor using an ultracentrifuge (Beckman). After centrifugation ...
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Comparative membrane proteomics reveals diverse cell regulators concentrated at the nuclear envelopebioRxiv - Cell Biology 2023Quote: ... The gradients then were centrifuged at 35,000 rpm (210,000g) for 1 h at 4°C with no brake in an SW41Ti rotor (Beckman Coulter). Two samples were collected from the nucleus gradient ...