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Citations for Beckman :
851 - 874 of 874 citations for 7 AMINO 1 3 NAPHTHALENEDISULFONIC ACID DISODIUM SALT since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... the extracts were added on top of layers in LCM buffer with 45% and 5% glycerol and ultracentrifuged at 77 000 x g for 1 h at +4°C (Beckman Coulter SW41Ti rotor). Viral nucleocapsids were recovered from the bottom of the ultracentrifuge tube ...
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bioRxiv - Biochemistry 2022Quote: ... The polymerized actin samples were pelleted down by ultracentrifugation at room temperature (45000 rpm for 1 hour, TLA-55 rotor, Beckman Coulter, Indianapolis, IN). The pellets were rinsed once with a 1 mL G-buffer and re-suspended into ice-cold 0.5 mL G-buffer ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Sequencing libraries for each replicate were generated via qPCR and sequencing libraries were purified and size selected using Ampure XP Beads at a 1.8:1 bead volume: library volume (Beckman Coulter, Brea, CA, USA). Library quality and transposition efficiency was accessed using a Fragment Analyzer and PROSize 2.0 (Agilent) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... the cell lysate was layered on top of a discontinuous OptiPrep® gradient (50 %, 35 %, 30 %, 20 %, and 15 %) and centrifuged at 135,000 g for 1 h at 4°C (Beckman L8-80M Ultracentrifuge, USA). The melanin pellet was recovered ...
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bioRxiv - Microbiology 2023Quote: ... The cells were then incubated for 30 minutes with the following antibodies: anti-HIV-1 core antigen (p24)-RD1 (Beckman Coulter catalog # 6604667), anti-TNFα PE/Dazzle 594 (BioLegend catalog # 502946) ...
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bioRxiv - Molecular Biology 2020Quote: ... bound antibodies were visualized using 100 µL of a 1:100 dilution of goat F(ab’)2 anti-human IgG coupled to phycoerythrin (Beckman Coulter, Brea, CA, USA) at 4°C for 30 min ...
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bioRxiv - Molecular Biology 2021Quote: The VHH-coding regions within parent libraries and 1-round target-enriched sublibraries were PCR amplified and purified using AMPure XP beads (Beckman Coulter, High Wycombe, UK). Then ...
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bioRxiv - Biochemistry 2020Quote: Sedimentation velocity experiments were performed using a ProteomeLab XL-1 analytical ultracentrifuge with the use of an AN60-Ti rotor and Epon-2 channel centerpieces (Beckman Coulter, Brea, CA, USA). Protein samples were diluted with SANS buffer to concentrations yielding an absorbance of 0.5 at 280 nm as measured with an 8454 UV–Vis spectrophotometer (Agilent ...
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bioRxiv - Microbiology 2022Quote: ... Reaction products were verified on a 1% agarose gel and purified using the AMPure XP magnetic Bead protocol (Beckman Coulter Inc., Brea, CA, USA). PCR2 was performed in a 25 μl reaction ...
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bioRxiv - Neuroscience 2019Quote: ... and 1□mM DTT) before a centrifugation process of 2 h 20 min at 30,000□× g (Beckman J-25 centrifuge with a J13.1 rotor). The supernatant was carefully aspirated and the extracted nuclei in a form of a pellet were resuspended in a nuclear storage buffer (15% sucrose ...
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bioRxiv - Systems Biology 2020Quote: ... and the resultant supernatant was ultracentrifuged at 100,000xg for 40 minutes at 4°C (rotor TLA 120.2; Beckman Coulter 343778 1 ml uncapped tubes).
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bioRxiv - Physiology 2021Quote: ... sEV were further isolated from lEV-depleted supernatants following a 100,000 xg ultracentrifugation step for 1 hour at 4°C (rotor MLA-50, Beckman Coulter Optima MAX-XP Ultracentrifuge) and two washes in NaCl before resuspension in NaCl.
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bioRxiv - Biochemistry 2024Quote: ... Triton X-100 was added to a 0.1% (v/v) and centrifuged for 45 minutes at 20,000 g at 1℃ (Beckman Coulter J2-21, JA 20 rotor). The supernatant was loaded onto a 5 ml HisTrap FF Crude column (Cytiva) ...
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bioRxiv - Neuroscience 2023Quote: ... PI (1 μl/100 μl) was added 5 minutes before starting the reading on the flow cytometer CytoFlex S (Beckman Coulter, Brea, CA, USA). Results were shown and analyzed through CytExpert flow cytometry analysis software.
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bioRxiv - Microbiology 2023Quote: The amplicons obtained with each of the strategies (using pools 1 and 2) were pooled in each group and purified with one volume of AMPure XP beads (Beckman Coulter, Brea, CA, USA). The beads were washed with 80% ethanol ...
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bioRxiv - Biophysics 2023Quote: ... Cell debris was removed by centrifugation at 20,000 rpm at 4°C for 1 h (Avanti® J-E centrifuge from Beckman Coulter, rotor: JLA 25.50). The supernatant was collected and loaded onto a pre-equilibrated 5 ml His-Trap FF crude column ...
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bioRxiv - Bioengineering 2023Quote: ... and a humidity of 85 % in 48-well Flowerplates containing 800 µL defined CGXII medium with 0.5 % (w/v) glucose inoculated to an OD600 of 1 by using the BioLector microbioreactor for 24 h (Beckman Coulter Life Sciences, Aachen, Germany). Increasing concentrations of ANT (final concentration of 0 ...
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bioRxiv - Neuroscience 2023Quote: ... The samples were spun for 1 hour at RT in a Beckman ultracentrifuge using SW 120 Ti rotor (Beckman Coulter, Inc., Brea, CA, USA) at 100,000xg ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were washed with 1 ml flow buffer once then resuspended in 200 μl flow buffer and measured using a CytoFLEX cytometer (Beckman Coulter, Jersey City, NJ).
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bioRxiv - Biochemistry 2024Quote: ... The samples were then subjected to high-speed ultracentrifugation (100000 g for 1 h) at 20 using an Optima TL-100 benchtop ultracentrifuge (Beckman Coulter, Indianapolis, IN, USA). AgADFs and α-actin alone were processed identically as a controls ...
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bioRxiv - Microbiology 2024Quote: ... was then added to the labelling reaction and incubated for additional 30 min at room temperature before labeled-virus purification through ultracentrifugation for 1 hour at 35,000 RPM using a rotor SW40Ti (Beckman Coulter Life Sciences, Brea, CA, USA), at 4 °C in a 6–30% OptiPrep (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2020Quote: ... Protein expression was induced with 0.4 mM Isopropyl β-D-1-thiogalactopyranoside and cells were grown at 37 °C overnight before harvesting by centrifugation at 4000 rpm (Beckman Coulter Avanti JXN-26; JLA8.1 rotor) at 10 °C for 10 min.
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bioRxiv - Biochemistry 2019Quote: ... Protein expression was induced with 0.4 mM Isopropyl β-D-1-thiogalactopyranoside and cells were grown at 25 °C overnight before harvesting by centrifugation at 4000 rpm (Beckman Coulter Avanti JXN-26; JLA8.1 rotor) at 4 °C for 15 min.
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bioRxiv - Microbiology 2024Quote: ... and stained intracellularly using PE-conjugated mouse anti-p24 mAb or using PE of FITC-conjugated mouse anti-p24 mAb (clone KC57; Beckman Coulter, Brea, CA, USA; 1:100 dilution). Cell-surface staining of infected primary MDMs cells was performed five days post-infection ...