Labshake search
Citations for Beckman :
851 - 900 of 1329 citations for 6 Hydroxymethyl 4 methoxy 5 methyl nicotinic acid methyl ester since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... The tubes were then centrifuged at 100,000 x g for 1 h at 4 ⁰C (TLA 55 rotor, Beckman Coulter; OptimaTM Max ultracentrifuge, Beckman Coulter). The pellets from the tubes were pooled together in 500 µl of 20 mM Tris-HCl pH 6.0 buffer and the volume was brought up to 1,500 µl using 20 mM Tris-HCl ...
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SARS-CoV2 associated secretion of nanoLuciferase reports on virus and Virus-Like Particle productionbioRxiv - Molecular Biology 2022Quote: ... the samples were pelleted through a 20% sucrose in 120 mM HEPES cushion at 100,000xg for 2hrs at 4 °C with 45 Ti or 70 Ti fixed-angle rotor (Beckman-Coulter) depending on the volume of the sample ...
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bioRxiv - Molecular Biology 2022Quote: ... The gradients were ultra-centrifuged for 3 h at 175,000g at 4°C in a SW41Ti rotor (Beckman-Coulter, USA) and analyzed by measuring the absorbance continuously at 260 nm using a piston gradient fractionator (Biocomp Instruments) ...
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bioRxiv - Molecular Biology 2022Quote: ... and spun at 40,000 rpm for 3 hours at 4°C using a SW 41 Ti Swinging-Bucket Rotor (Beckman Coulter). Sucrose gradient fractionation was performed by using a Brandel Density Gradient Fractionation System ...
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bioRxiv - Molecular Biology 2022Quote: ... and the sucrose cushion was ultra-centrifuged for 1.5 hours at 80000 rpm and 4°C in TLA-100 rotor (Beckman Coulter). The pellet was resuspended in 100 µL of buffer F (50 mM HEPES-KOH pH 7.4 ...
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bioRxiv - Cell Biology 2022Quote: ... and the post-nuclear supernatant was loaded onto an 8-step discontinuous sucrose density gradient (HEPES-buffered 0.2–2 M sucrose) and centrifuged at 55,000 rpm for 2h at 4 °C using an MLS50 rotor (Beckman Coulter). Extracellular acidification rate (ECAR ...
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bioRxiv - Molecular Biology 2024Quote: ... the rest was loaded on top of sucrose gradient and ultracentrifuged (30000 rpm, 4°C, 18h, stop DECEL, slow, SW40/1Ti swinging bucket rotor, Optime L-90K, Beckman). 24 fractions were taken from top to bottom of the tube as 500 µL aliquots and precipitated by incubation with 50 µL of 0.15% sodium deoxycholate for 10 min at RT ...
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bioRxiv - Genomics 2023Quote: ... 1x protease inhibitor cocktail EDTA-free) by spinning at 38,000 rpm at 4 °C in a SW-41 Ti rotor (Beckman Coulter). Monosome pellets were resuspended in 400 µL lysis buffer and rocked overnight ...
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bioRxiv - Biophysics 2024Quote: ... and subjected to ultracentrifugation at 384,492□g for 45□min at 4□°C (Beckman Coulter Optima MAX, TLA-100 rotor), stored at −80 °C after flash-frozen with liquid nitrogen for crystallization trials ...
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bioRxiv - Biochemistry 2024Quote: ... The protein samples were carefully applied to the gradient and centrifuged for 16 h at 130,000 g at 4 °C (Optima XPN-80 Ultracentrifugation from Beckman Coulter). 200 µl fractions were taken from the top of the gradient and the glutaraldehyde quenched with 20 mM Tris pH 7.5 ...
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bioRxiv - Biochemistry 2024Quote: ... The homogenate was clarified by centrifugation for 30 min at 70,560 × g at 4°C in an Optima XPN Ultracentrifuge (Beckman Coulter) using a Ti-45 rotor ...
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bioRxiv - Molecular Biology 2024Quote: ... Lysates were cleared by centrifugation for 1 h at 45,000 rpm at 4°C (Optima XPN-80 Ultracentrifuge with 70 Ti, Beckman Coulter). HTP-tagged proteins were immunoprecipitated with 600 µl pre-washed IgG-sepharose 6 fast flow affinity resin (GE Healthcare ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were centrifuged at 30,000 xg for 45 min at 4 °C using a SW41 Ti swinging bucket rotor (Beckman Coulter) before the supernatant was decanted ...
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bioRxiv - Neuroscience 2024Quote: ... The homogenate was centrifuged (45 min, 16 000 rpm, 4°C, JLA 16.250 rotor, Beckman-Coulter Centrifuge Avanti J25-01) and the supernatant subjected to Ni-NTA affinity purification (HIS-Select Nickel Affinity Gel (Sigma Aldrich)) ...
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bioRxiv - Cell Biology 2024Quote: ... Gradients were centrifuged at 36,000 rpm for 2 h at 4 °C in an Optima L-100XP ultracentrifuge (Beckman–Coulter). Following centrifugation ...
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bioRxiv - Neuroscience 2024Quote: ... The supernatant was ultracentrifuged at 80,000 × g for 1 h at 4°C using a type 50 Ti rotor ultracentrifuge (Beckman Coulter). The supernatant ...
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bioRxiv - Biochemistry 2023Quote: ... The gradient was centrifuged for 2.30 hours at 4 °C and 111,000 ×g in a swing-out rotor (Beckman- Coulter, SW41). The brownish lysosome-enriched fraction (∼ 1ml ...
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bioRxiv - Cancer Biology 2023Quote: ... The gradients were ultracentrifuged at 35,000 rpm for 2 hrs at 4°C using an SW41 Ti rotor (Beckman Coulter). The gradients were then displaced into a UA-6 continuous UV detector (Teledyne ISCO ...
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bioRxiv - Cell Biology 2023Quote: ... Small EVs were isolated by ultracentrifugation at 120,000 x g and 4°C for 120 min using Optima XE-90 equipment with a SW32Ti rotor (Beckman Coulter). Supernatants were discarded using a pipette ...
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Centriole and transition zone structures in photoreceptor cilia revealed by cryo-electron tomographybioRxiv - Cell Biology 2023Quote: ... OptiPrep step-gradient and centrifuged for 60 min at 19,210 × g at 4 °C in a TLS-55 rotor (Beckman Coulter). The ROS band was collected with an 18G needle ...
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bioRxiv - Cell Biology 2023Quote: ... The gradient was centrifuged at 4 °C and 100,000 ˗ g for 18 h (Optima L-80 XP Ultracentrifuge with Swing Rotor (Beckman Coulter)) ...
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bioRxiv - Developmental Biology 2023Quote: ... The resulting supernatant was subjected to ultracentrifugation at 120,000 × g at 4°C for 2 h (Optima Max TL, Beckman Coulter). Pellets were resuspended in 1 ml cold PBS and centrifuged at 120,000 × g at 4°C for 2 h ...
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bioRxiv - Immunology 2023Quote: ... and purified and concentrated by ultracentrifugation on a 20% sucrose cushion (24,000 rpm and 4°C for 2 hours with a SW32Ti or SW28 rotor (Beckman Coulter)) ...
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bioRxiv - Neuroscience 2023Quote: ... and samples were centrifuged at 24,000 rpm at 4°C for 1h in a SW41Ti Swinging-Bucket Rotor (Beckman Coulter). After discarding the supernatant ...
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bioRxiv - Cell Biology 2023Quote: ... The samples were sedimented by velocity centrifugation at 36,000 x rpm for 2 h at 4°C using SW40Ti rotor in Optima L-80XP ultracentrifuge (Beckman Coulter). Absorbance at 254 nm was measured from lower to higher sucrose gradients using an ISCO gradient fractionation system and the optical density at 254 nm was continuously recorded with a Foxy JR Fractionator (Teledyne ISCO).
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bioRxiv - Cell Biology 2024Quote: ... The same amounts of proteins were loaded on a double sucrose cushion (20% and 30% sucrose) and centrifuged at 190,000 g for 2 h at 4 °C in an Optima L-100XP ultracentrifuge (Beckman–Coulter). Extracts were incubated with pre-washed anti-Flag beads (Sigma ...
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bioRxiv - Neuroscience 2024Quote: ... Digested samples were ultracentrifuged at 100,000x g for 1 h at 4 °C using a TLA-55 rotor (Beckman Coulter) to concentrate the insoluble ...
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bioRxiv - Immunology 2023Quote: ... virus solutions were clarified of debris by centrifugation at 10,000 rpm at 4°C for 30 minutes in a chilled SW32Ti rotor (Beckman Coulter), followed by pelleting through a 30% cold sucrose solution at 25,000 rpm for 90 minutes ...
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bioRxiv - Microbiology 2023Quote: ... Medium was cleared from EVs by ultracentrifugation at 100,000 ×g for 18 h at 4°C in a LE-80 ultracentrifuge (Beckman Coulter) with a 60Ti rotor [13] ...
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bioRxiv - Microbiology 2023Quote: ... Cells were washed twice with 200µl BD 1X Perm/Wash solution and stained 1h at 4°C with anti-HIV-1 p24 (RD1/PE, Beckman Coulter #6604667 ...
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bioRxiv - Biochemistry 2023Quote: ... The sucrose density gradient was centrifuged at 50,000 rpm for 3 hours at 4°C in a TLS-55 swinging bucket rotor (Beckman Coulter) with minimum acceleration up to 5,000 RPM and deceleration with no break ...
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bioRxiv - Biochemistry 2023Quote: ... The diluted extract was then centrifuged at 31,200 rpm for 30 minutes at 4°C in a Type 70 Ti rotor (Beckman Coulter). The supernatant was filtered through a 0.45 µm syringe filter (Millex-GP PES membrane (Millipore SLHP033RS ...
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bioRxiv - Biochemistry 2023Quote: ... The lysate was centrifuged at 40,000 rpm for 60 min at 4°C (XPN-100 ultracentrifuge, Type 45 Ti rotor, Beckman, USA). The supernatant was filtered through a 0.45 μm filter (Pall ...
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bioRxiv - Bioengineering 2023Quote: ... For performing cryogenic transmission electron microscopic imaging EVs were centrifuged and washed in PBS at 100,000 x g for 3 hours at 4°C in a SW41 Ti rotor (Beckman Coulter).
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bioRxiv - Bioengineering 2023Quote: ... The final supernatant was centrifuged at 100,000g at 4°C for 70 min using an ultracentrifuge (Optima MAX-XP Tabletop Ultracentrifuge, Beckman Coulter). The pellet was either used immediately or stored dry at −80°C ...
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bioRxiv - Biochemistry 2023Quote: ... and the mixtures were then layered under a 20-40% iodixanol discontinuous gradient before to be centrifuged at 260,000 x g for 16h at 4°C using an SW41 Ti rotor (Beckman Coulter). After spinning ...
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bioRxiv - Cancer Biology 2023Quote: ... Filtered supernatants were concentrated by ultracentrifugation at 22.000 rpm for 2h at 4°C with OptimaTM L-90K ultracentrifuge (Beckman Coulter) and stored at −80°C (never refrozen).
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bioRxiv - Cell Biology 2023Quote: ... and pelleted at 100,000xg for 20 min at 4 °C using fixed angle rotor (TLA-100 rotor, Beckman Coulter, 349481) in a benchtop ultracentrifuge (OptimaTL ...
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bioRxiv - Cell Biology 2023Quote: ... Equilibrated tubes were ultra-centrifuged at 100,000xg for 1h at 4°C using a swinging-bucket rotor (SW 32Ti, Beckman Coulter, 369650; Optima XE, Beckman Coulter). Phagosome-containing beads float at the interface between the 10% and 25% layers ...
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bioRxiv - Biophysics 2023Quote: ... The lysate was clarified by centrifugation at 40,000 rpm for 35 min at 4°C (Type 45 Ti Rotor, Beckman Coulter). The resulting supernatant was mixed with 1ml pre-washed IgG Sepharose 6 FastFlow beads (Cytiva ...
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bioRxiv - Biophysics 2023Quote: ... Centrifugation was performed at 154,300 × g for 24 h at 4◦C (SW 41 Ti swinging-bucket rotor, Beckman Coulter). The separated bands were collected dropwise from the bottom of the tube ...
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bioRxiv - Cell Biology 2023Quote: ... The concentrated CFS was then ultracentrifuged at 100,000 ˗ g and 4 °C for 3 h (Optima L-80 XP Ultracentrifuge with Type 70.1 Ti Fixed-Angle Titanium Rotor (Beckman Coulter)) ...
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bioRxiv - Biochemistry 2024Quote: ... The homogenate was clarified by centrifugation for 30 min at 70,560 × g at 4°C in an Optima XPN Ultracentrifuge (Beckman Coulter) using a Ti-45 rotor ...
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bioRxiv - Microbiology 2022Quote: ... clarified using 0.2 µm filter and pelleted at 54,000 rpm (180,000 ×g) for 2 hours at 4 °C in a TLA-55 rotor in an Optima Max-XP tabletop ultracentrifuge (Beckman). The supernatant was removed ...
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bioRxiv - Biochemistry 2022Quote: ... A soluble fraction was obtained after centrifugation of the lysate at 19,000 rpm for 40 min at 4 °C performed using Avanti J-E with a rotor JA-20 (BECKMAN COULTER) to remove insoluble debris.
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Dynamic states of eIF6 and SDS variants modulate interactions with uL14 of the 60S ribosomal subunitbioRxiv - Biochemistry 2022Quote: ... Ribosomes were pelleted by centrifugation at 63,000g for 16–19 hrs at 4°C using a Type 80 Ti rotor (Beckman Coulter). For all ultracentrifugation steps indicated here ...
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Dynamic states of eIF6 and SDS variants modulate interactions with uL14 of the 60S ribosomal subunitbioRxiv - Biochemistry 2022Quote: ... were pelleted by centrifugation at 63,000g for 20 hrs at 4°C in a Type 80 Ti rotor (Beckman Coulter) as described above ...
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bioRxiv - Cell Biology 2022Quote: ... OptiPrep step-gradient and centrifuged for 60 min at 19,210 × g at 4 °C in a TLS-55 rotor (Beckman Coulter). The ROS band was collected with a 18G needle ...
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bioRxiv - Cell Biology 2022Quote: ... The lysate was dounced for 10 strokes on ice and cleared via centrifugation at 80K rpm for 10 minutes at 4°C using a TLA 110 rotor (Beckman) in a tabletop Beckm an ultracentrifuge ...
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bioRxiv - Plant Biology 2022Quote: ... in a 12.5 mL ultracentrifuge tube and centrifuged for 1 h at 4°C at 200,000 × g in a swinging bucket rotor (Sorval SW-41 Ti; Beckman Coulter), with moderate acceleration and deceleration ...