Labshake search
Citations for Beckman :
851 - 900 of 1839 citations for 1 2 4 4 4 Pentachloropentafluorobutane since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2022Quote: ... The lysate was dounced for 10 strokes on ice and cleared via centrifugation at 80K rpm for 10 minutes at 4°C using a TLA 110 rotor (Beckman) in a tabletop Beckm an ultracentrifuge ...
-
bioRxiv - Microbiology 2022Quote: ... purified by ultracentrifugation through a sucrose cushion (25% weight/volume in Tris-NaCl-EDTA buffer) for 75 min at 4°C and 28,000 rpm using a SW 32 TI rotor (Beckman Coulter), resuspended in serum-free DMEM medium and stored in small aliquots at −80°C ...
-
bioRxiv - Microbiology 2022Quote: ... Pseudotypes and VLPs were then concentrated by ultracentrifugation (20,000 RPM, 4 ºC, 1.5h, Beckman Coulter Optima XPN-100, SW32Ti rotor) through a 20% (w/v ...
-
bioRxiv - Microbiology 2022Quote: ... 20 mL of the filtrate was then ultracentrifuged at 150,000 × g at 4°C for 3 h (Beckman Coulter, USA) through a 20% (w/v ...
-
bioRxiv - Microbiology 2022Quote: ... at 20 KPSI at 4°C and then centrifuged at 19 000 RPM in a JA-25.50 rotor (Beckman Coulter) for 30 minutes at 4°C ...
-
bioRxiv - Molecular Biology 2023Quote: ... using 10-40 % sucrose gradients in buffer A after 3 hours of centrifugation at 35,000 rpm and 4 °C in SW41 rotor (Beckman Coulter). To isolate ribosomes for structural analysis ...
-
bioRxiv - Neuroscience 2023Quote: ... followed by ultracentrifugation at 100,000 x g for 60 minutes at 4°C (SW 41 Ti Rotor, Beckman Coulter Inc). For size-exclusion chromatography (SEC ...
-
bioRxiv - Neuroscience 2023Quote: ... The gradients were centrifuged for 18 h at 100,000 xg at 4 °C in a SW 40 rotor (Beckman Coulter). 500 µl fractions were collected from the top of the ultracentrifuge tubes and analyzed by WB analysis.
-
bioRxiv - Cell Biology 2022Quote: ... Lysates were cleared by centrifugation for 30 min at 4°C at 18,000 rpm (SS-34 rotor; Beckman-Coulter, USA).
-
bioRxiv - Cell Biology 2023Quote: ... down to 6 ml and ultracentrifuged at 10,000xg for 16 min at 4°C in MLA-80 rotor (Beckman Coulter). The pellet was resuspended and washed with PBS at the same conditions (volume ...
-
Comparative membrane proteomics reveals diverse cell regulators concentrated at the nuclear envelopebioRxiv - Cell Biology 2023Quote: ... The whole cell homogenate was layered on top of 2 ml shelf of 0.8 M sucrose in HB and centrifuged at 2000 rpm for 10 min at 4°C in a JS5.2 rotor (Beckman Coulter) to yield a low speed nuclear pellet and a postnuclear supernatant ...
-
bioRxiv - Bioengineering 2023Quote: ... The final supernatant was centrifuged at 100,000g at 4°C for 70 min using an ultracentrifuge (Optima MAX-XP Tabletop Ultracentrifuge, Beckman Coulter). The pellet was either used immediately or stored dry at −80°C.
-
bioRxiv - Biochemistry 2023Quote: ... The reaction mixture was applied onto the top of the gradient solution and was centrifuged at 41,000 rpm at 4 °C for 17 hrs using an SW41 Ti rotor (Beckman Coulter). The gradient was fractionated using a Piston Gradient Fractionator (BioComp) ...
-
bioRxiv - Cell Biology 2023Quote: ... and then centrifuged for 150 min at 39,000 rpm and 4°C in an Optima L-100XP Ultracentrifuge (Beckman-Coulter) using a SW41Ti rotor ...
-
bioRxiv - Biophysics 2023Quote: ... at 4°C until equilibrium was reached using an An Ti 60 rotor in an XL-I analytical ultracentrifuge (Beckman), run using ProteomeLab XL-A/XL-I (version 6.2) ...
-
bioRxiv - Plant Biology 2024Quote: ... and centrifuged at 10,000 x g for 30 min at 4°C using a TLA100.3 fixed angle rotor and an Optima TLX Ultracentrifuge (Beckman Coulter).
-
bioRxiv - Microbiology 2024Quote: ... sucrose gradients prepared with buffer C and centrifuged for 16 h at 71,935 g at 4 °C in a SW28 rotor (Beckman Coulter). Gradients were fractionated on a Biocomp Piston Gradient Fractionator (BioComp Instruments ...
-
bioRxiv - Neuroscience 2024Quote: ... The discontinuous sucrose gradient was centrifuged at 242,000 g for 47 min at 4 °C in a vertical rotor (VTi50 rotor, Beckman Coulter). The bulk GC fraction (GCF ...
-
bioRxiv - Microbiology 2024Quote: ... Samples were added to the top of a 60%-30%-10% sucrose/PBS gradient and centrifuged at 16,000 x g for 4.5 h at 4°C with an SW32 Ti rotor (Beckman, Brea, CA). Virus bands at the 60%-30% sucrose interface were collected via tube side puncture ...
-
bioRxiv - Microbiology 2024Quote: ... tepidum culture was centrifuged at 10,000 x g for 10 min at 4°C in a JA-14 rotor (Beckman Coulter), and the supernatant was centrifuged again at 12,000 x g to remove cells and large debris ...
-
bioRxiv - Molecular Biology 2024Quote: ... The gradient was centrifuged at 54,000 rpm for 3 h at 4 °C using a SW55 Ti rotor (Beckman Coulter). After centrifugation ...
-
bioRxiv - Neuroscience 2024Quote: ... and samples were centrifuged at 24,000 rpm at 4°C for 1h in a SW41Ti Swinging-Bucket Rotor (Beckman Coulter). After discarding the supernatant ...
-
bioRxiv - Molecular Biology 2024Quote: ... and EVs were pelleted by ultracentrifugation for 60 minutes at 100,000 x g at 4 ° C in an SW40.Ti rotor (Beckman Coulter). After centrifugation ...
-
bioRxiv - Biochemistry 2024Quote: ... Lysates were clarified by ultracentrifugation at 4℃ at 184,000 x (g) in a 70Ti rotor for 50 minutes (Beckman Coulter). After affinity chromatography with StrepTactin SuperFlow resin (IBA) ...
-
bioRxiv - Biochemistry 2024Quote: ... A crude ribosome pellet was obtained by centrifugation for 2h at 200,000 × g at 4 °C (70Ti rotor, Beckman Coulter) and solubilized in HMA6/30 for 16h at 4°C ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... EVs were purified from concentrated HES by ultracentrifugation at 100,000 x g for 90 mins at 4 °C in polyallomer tubes (Beckman Coulter) in a SW40 rotor (Beckman Coulter) ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... and pelleted each time by ultracentrifugation at 100,000 x g for 70 mins at 4 °C in polyallomer tubes (Beckman Coulter) in a SW40 rotor (Beckman Coulter) ...
-
bioRxiv - Biochemistry 2024Quote: ... Lysates were clarified by ultracentrifugation for 35 minutes at 4℃ at 70,000 x (g) in a 45Ti rotor (Beckman Coulter). Constructs containing N-terminal TOG domains (TOG1 ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... containing cell nucleus and other heavy organelles was discarded and supernatant was centrifuged for 30 min at 48,000 × g at 4 °C (Beckman Avanti J-251 Ultra-centrifuge; Beckman Coulter). The supernatant was discarded ...
-
bioRxiv - Biochemistry 2024Quote: ... Equivalent amounts of cell lysates prepared as described above were loaded onto the sucrose gradients and spun at 35,000 g for 3 hours at 4°C in a SW41 rotor (Beckman Coulter). After centrifugation the polysomes were fractionated and absorbance was monitored at 254 nm for RNA and 680 nm for GFP using the TriaxTM Flowcell (BioComp Instruments) ...
-
bioRxiv - Immunology 2024Quote: ... and centrifuged at 100,000 x g at 4°C for 90 min using the Optima™ XPN-80 Ultracentrifuge from Beckman Coulter ...
-
bioRxiv - Biophysics 2024Quote: ... the sample was separated by sucrose gradient ultracentrifugation with the gradients of 10 to 30 % sucrose and 0 to 0.2 % glutaraldehyde at 97,311 g at 4°C for 18hrs using an Optima™ XL-100K ultracentrifuge (Beckman Coulter). The fractions containing the complex were pooled and the sucrose was removed by using Amicon Ultra Centrifugal Filter ...
-
bioRxiv - Cancer Biology 2024Quote: ... Samples were subjected to ultracentrifugation of 260,000 x g for 16 hours at 4°C using the Optima XPN-80 and SW40Ti rotor (Beckman Coulter). Twelve 1-mL fractions were collected from the top and subjected to immunoblotting.
-
bioRxiv - Cell Biology 2024Quote: ... The lysate was clarified at 150,000 rcf for 30 min at 4°C using a Type 45 Ti rotor (Beckman Coulter). The supernatant was incubated with 3 mL IgG Sepharose 6 Fast Flow beads (Cytiva ...
-
bioRxiv - Cell Biology 2024Quote: ... The lysate was clarified at 503,000 rcf for 45 min at 4°C using a Type 70 Ti rotor (Beckman Coulter). The supernatant was incubated with 3 mL IgG Sepharose 6 Fast Flow beads (Cytiva ...
-
bioRxiv - Plant Biology 2021Quote: ... the samples were centrifuged at 35 000 RPM for 18 hours at 4°C in a 70Ti rotor and L8-M ultracentrifuge (Beckman Coulter). The ribosome pellets were then gently washed with RNase-free water and resuspended in 300 μl of resuspension buffer (100 mM Tris·HCl (pH 8.0) ...
-
bioRxiv - Molecular Biology 2021Quote: ... The gradient was ultracentrifuged at 55,000 rpm for 55 min at 4 °C with max acceleration and slow deceleration using an Optima LE-80K Ultracentrifuge and SW55Ti rotor (Beckman Coulter). The sucrose gradient was fractionated according to the number of associated ribosomes as determined by the profile of the absorbance at 254 nm using a Density Gradient Fractionation System (Brandel ...
-
bioRxiv - Neuroscience 2021Quote: ... This was incubated for 1 hour at room temperature while rotating before centrifugation at 100,000 x g for 90 minutes at 4°C using the Optima™ MAX-XP Ultracentrifuge (Beckman Coulter). The supernatant was then collected (sarkosyl-soluble fraction) ...
-
bioRxiv - Molecular Biology 2021Quote: ... pombe cells were pelleted down for 10 min at 5000 rpm at 4°C (Rotor: F10-6X500, FiberLite Beckman J2-MC). The pellet was washed with buffer 1 (HEPES- 20 mM ...
-
bioRxiv - Bioengineering 2022Quote: ... The 60-70 mL were then divided equally into two 50-mL centrifuge tubes and spun down outside the anaerobic chamber at 4 °C and 3500 rpm for 10 min using an Allegra 64R ultracentrifuge (Beckman Coulter). Next ...
-
bioRxiv - Bioengineering 2022Quote: ... Each 250 mL culture was divided equally into six 50-mL centrifuge tubes and spun down at 4 °C and 3500 rpm for 10 min using an Allegra 64R centrifuge (Beckman Coulter). The supernatant was discarded and 6 mL of ice-cold 10% glycerol was used to wash and combine the six cell pellets into one suspension ...
-
bioRxiv - Genomics 2020Quote: ... Lentiviral supernatant was collected at 48 and 72hrs post-transfection and subsequently concentrated by ultracentrifugation (24,000 rpm, 4°C, 2hrs) (Beckman Coulter SW32).
-
bioRxiv - Genetics 2020Quote: ... The homogenates were incubated on ice for 15–20 min and then centrifuged at 3000xg for 10 min at 4°C in a GPR Data Settop centrifuge (Beckman, Inc.). The supernatant solutions were centrifuged at 15,800xg for 15 min at 4°C in a microcentrifuge (Model 5042 ...
-
bioRxiv - Plant Biology 2020Quote: ... The loaded density gradients were centrifuged at 33,000 g for 14.5 h at 4°C using a six-position SW41 Ti rotor (Beckman Coulter, Kerfeld, Germany). The positions of ribosome complexes varied slightly with each centrifugation run that comprised a set of six samples ...
-
bioRxiv - Bioengineering 2020Quote: ... Ultracentrifugation was performed at 100,000 x g at 4 C for 45 min in an Optima MAX-XP ultracentrifuge (Beckman Coulter, Inc.). Supernatant was discarded ...
-
bioRxiv - Biochemistry 2020Quote: ... in BC300N buffer and centrifuged for 4hr at 55,000rpm at 4°C in a TLS-55 rotor (with Beckman 356860 adaptors). 40µl fractions were collected by pipetting from the top ...
-
bioRxiv - Biophysics 2021Quote: Purified 3C DNA from each sample was sonicated to 200-400 bp with a Soniprep 150 probe sonicator at 4°C and purified with a standard Ampure XP Bead protocol (Beckman Coulter) using a 1/1.5 DNA to bead ratio ...
-
bioRxiv - Neuroscience 2020Quote: ... The supernatant was then collected and subjected to ultracentrifugation at 100,000 x g for 70 min at 4°C using an Optima XPN-80 ultracentrifuge with SW 32 Ti swinging rotor (Beckman Coulter). Pellets were washed in PBS 1X and EVs were subjected to an additional centrifugation at 100,000 x g for 30 min at 4°C using an Optima MAX ultracentrifuge ...
-
bioRxiv - Neuroscience 2020Quote: ... centrifuged at 100,000 g for 70 min at 4°C using an Optima XPN-80 ultracentrifuge with SW 32 Ti swinging rotor (Beckman Coulter) and then processed for further analyses.
-
bioRxiv - Plant Biology 2021Quote: ... Syringes were placed inside 50-mL tubes and centrifuged for 20 min at 700g with slow acceleration (4°C, JA-14 rotor, Avanti J-20 XP Centrifuge; Beckman Coulter). The apoplastic wash fluid was then filtered through a 0.22 μm membrane and centrifuged at 10,000g for 30 minutes to remove any remaining large particles ...