Labshake search
Citations for Beckman :
801 - 850 of 891 citations for hsa mir 940 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... the PCR products were pooled in a certain ratio and purified with 1x AMPure XP beads (Beckman Coulter A63881).
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bioRxiv - Cell Biology 2024Quote: ... The mutation was confirmed by genomic PCR followed by Sanger sequencing using CEQ8000 (Beckman Coulter Inc, Brea, CA, USA).
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bioRxiv - Genomics 2024Quote: ... Samples were then pooled to equimolar concentration and cleaned using the Agencourt AMPure XP PCR purification system (Beckman Coulter) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... according to the manufacturer’s protocol with 12 cycles of PCR followed by two rounds of clean up with 1.3X Agencourt AMPure XP beads (Beckman Coulter). The resulting double-stranded DNAs were fragmented by Covaris sonicator (S220 ...
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bioRxiv - Genomics 2019Quote: ... and the combined pool of PCR products was purified in a single tube using Ampure XP beads (Beckman-Coulter, A63880) at 1:1 (V/V ...
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bioRxiv - Microbiology 2022Quote: ... DNA library preparation was performed using the Illumina Nextera protocol and PCR clean up was performed using AMPure beads (Beckman). Multiplexed samples were then run on the MiSeq (Illumina) ...
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bioRxiv - Microbiology 2020Quote: ... and the resulting cDNA was PCR amplified (11 cycles) and purified using the Agencourt AMPure XP kit (Beckman Coulter Genomics). For Illumina NextSeq sequencing ...
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bioRxiv - Neuroscience 2020Quote: ... sgRNA amplification was performed using a two-step PCR strategy and purified using Agencourt AMPure XP (A63880; Beckman Coulter UK), Illumina sequencing (19-bp single-end sequencing with custom primers on the HiSeq2500 Rapid Run ...
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bioRxiv - Neuroscience 2020Quote: ... PCR products were then cleaned up by double-sized selection by using Ampure beads (Agencout AMPure XP, Beckman Coulter, #A63880) to remove large and small DNA fragments ...
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bioRxiv - Physiology 2019Quote: ... Agencourt AMPure XP PCR purification kit (5 ml Beckman Coulter Part No. A63880; 60 ml Beckman Coulter Part No. A63881) was used ...
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bioRxiv - Immunology 2021Quote: ... The first PCR reaction was purified using the AMPure Size Select Magnetic Bead Kit at a ratio of 0.6X (Beckman Coulter). Amplicon libraries were then prepared according to the Pacific Biosciences Multiplex SMRT Sequencing protocol and sequenced on a Pacific Biosciences Sequel platform (Pacific Biosciences ...
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bioRxiv - Microbiology 2022Quote: ... PCR products were confirmed through visualization in 1% agarose gel and purified using AMPure XP beads (Beckman Coulter, Indianapolis, U.S.A.). PCR libraries were pooled together and sent to the Centre d’expertise et de services Genome Québec (Montréal ...
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bioRxiv - Microbiology 2022Quote: ... PCR products from the same samples were then pooled and cleaned using AMPure XP beads (Beckman Coulter, Pasadena, CA, USA). Concentration of PCR products was assessed using Qubit technology (Life Technologies ...
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bioRxiv - Microbiology 2023Quote: ... PCR products were pooled and purified using size-selective SPRIselect magnetic beads (0.8 left-sized, Beckman Coulter, Brea, CA, USA). The library was then diluted to 6pM for sequencing ...
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bioRxiv - Microbiology 2023Quote: ... This PCR amplification was followed by another purification using the Agencourt® AMPure® XP beads (#A63881, Beckman Coulter, UK). A ratio of 6.5 of Solid Phase Reversible Immobilisation beads (SPRI ...
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bioRxiv - Genetics 2023Quote: ... Libraries were amplified using 5-7 cycles of PCR using KAPA HiFi DNA polymerase and purified with 1.1X SPRI (Beckman Coulter). Nucleosome profiles were manually inspected using a Tapestation with the D1000 Screen Tape system (Agilent) ...
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bioRxiv - Biochemistry 2023Quote: ... Eight 50 μL PCR reactions were combined in a 1.5 mL Eppendorf tube and mixed with 320 μL Ampure XP beads (Beckman Coulter) (0.8:1 ratio ...
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bioRxiv - Immunology 2023Quote: ... the PCR plate was briefly centrifuged and 25 µl of room-temperature AMPure XP beads (1:1 ratio; Beckman Coulter) was added to each well and incubated for 8 minutes at room temperature ...
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bioRxiv - Evolutionary Biology 2023Quote: ... viral cDNA was purified either by standard PCR reaction desalting columns or with a 0.45X volume of AMPure XP beads (Beckman Coulter). Nextera XT libraries were generated with 1 ng of cDNA material ...
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bioRxiv - Plant Biology 2024Quote: The replicates of the second PCR were pooled for each sample and purified with Agencourt AMPure XP beads (Beckman Coulter) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: ... Amplicon PCR products were then purified by a 0.65X + 1.05X double size selection with AMPure XP Beads (Beckman Coulter, A63882). Purified amplicons were then pooled based on the concentrations measured by the Quant-iT™ dsDNA Assay HS Kit (Thermo Fisher ...
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bioRxiv - Molecular Biology 2024Quote: ... The initial PCR products of rbcL F3R3 were purified using an Agencourt AMPure XP kit (Beckman Coulter, Fullerton, CA, USA). To construct the DNA libraries for the second PCR ...
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bioRxiv - Genetics 2023Quote: ... each DNA sample was amplified in three reactions each containing 66 ng of DNA.1st stage PCR products for each sample were then pooled and purified using the Agencourt AMPure XP system (Beckman Coulter ...
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bioRxiv - Molecular Biology 2019Quote: ... Size selection purifications following the ligation and amplification PCR steps were performed with 1x and 0.9x reaction volumes of Agencourt AMPure XP beads (Beckman Coulter - A63880). Purified libraries were combined as a 12 sample equimolar pool containing the indexes 1-12 and sequenced on an Illumina NextSeq on a single high-output flow cell (single-end 75 bp reads).
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bioRxiv - Molecular Biology 2019Quote: ... Size selection purifications following the ligation and amplification PCR steps were performed with 1x and 0.9x reaction volumes of Agencourt AMPure XP beads (Beckman Coulter - A63880). Purified libraries were combined as an 8 sample equimolar pool containing the indexes 5-12 and sequenced on an Illumina NextSeq on a single high-output flow cell (paired-end 75 bp reads).
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bioRxiv - Neuroscience 2021Quote: ... 12 cycles of PCR were performed for library amplification and libraries were cleaned and size selected (Ampure XP beads; A63880, Beckman Coulter) as described (Sanz and Chédin ...
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bioRxiv - Cell Biology 2022Quote: ... The cDNA amplification from single cells was performed by PCR for 21 cycles and the amplified products were purified using AMPure XP paramagnetic beads (Beckman Coulter).
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bioRxiv - Genomics 2020Quote: ... Equal quantities of the four PCR products from each mosquito pool were mixed and purified using Agencourt AMPure XP (Beckman Coulter).
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bioRxiv - Neuroscience 2020Quote: ... A total of 25 μl of the PCR product was cleaned using 1X Agencourt AMPure XP beads (Beckman Coulter, Brea, USA) and eluted in 25 μl TE buffer ...
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bioRxiv - Cell Biology 2020Quote: ... The first PCR reaction was performed for 15 cycles with 60 °C annealing and 30 s elongation and then purified with AMPure XP PCR beads (Beckman Coulter). The second PCR was performed for 15 cycles for wild type-specific and for 21 cycles for tag-specific amplicons respectively using 60°C annealing and 30 s elongation ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were purified using 1.8X Agencourt AMPure XP PCR Purification beads according to the manufacturer’s instructions (Beckman Coulter, cat no A63881). The sheared DNA samples were quantified and qualified on a BioAnalyzer system using the DNA7500 assay kit (Agilent Technologies cat no ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were tagged using barcodes NB01 to NB05 of the Native Barcoding Expansion 1-12 PCR-free kit (EXP-NBD104, ONT) and purified using Agencourt AMPure XP beads (Beckman Coulter). The barcoded samples were quantified using a Qubit fluorometer and pooled equimolarly to reach 700 ng in 65 μL ...
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bioRxiv - Molecular Biology 2020Quote: ... The library was amplified with 9 PCR cycles and cleaned with 0.9x AMPure® XP beads (A63881, Beckman Coulter, CA, USA) to enrich for DNA fragments shorter than 50nt ...
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bioRxiv - Genomics 2019Quote: ... The resulting PCR reactions were pooled and purified (first using the minElute PCR purification kit of Qiagen, followed by an extra purification step using AMPureXP beads of Beckman Coulter), and the amplified cDNA quantified on a BioAnalyzer High Sensitivity Chip (Agilent) ...
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bioRxiv - Molecular Biology 2020Quote: ... The target library derived from the oocyte RNA polymerase II transcripts was PCR-amplified (15 cycles for P14 WT, 16 cycles for P14 Tbpl2−/− mutant) and purified using AMPure beads (Beckman Coulter) to remove short PCR artifacts (< 200bp ...
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bioRxiv - Molecular Biology 2021Quote: ... Indexing PCR amplicons were pooled (4◻μl of each) and purified using AMPure XP beads (Beckman Coulter, California, United States) according to manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: The obtained PCR products were purified and filtered by using AMPure XP magnetic beads with double size selection (Beckman Coulter, Inc.). In brief ...
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bioRxiv - Microbiology 2019Quote: ... PCR products were verified with a 2% agarose gel electrophoresis and purified using AMPure XP bead (Beckman Coulter, Brea, CA, USA). The purified amplicon library was quantified and sequenced on the MiSeq Platform (Illumina ...
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bioRxiv - Genetics 2020Quote: ... PCR was then analyzed on an agarose gel and DNA was cleaned up using Ampure XP beads (Beckman Coulter, cat. A63881). For this the four PCR reactions were pooled resulting in 100 μL ...
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bioRxiv - Immunology 2021Quote: ... The 1st-round PCR product was cleaned up and followed by a double size selection step using SPRIselect reagent (Beckman Coulter) to separate protein library from cDNA library.
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bioRxiv - Neuroscience 2021Quote: ... 50 μL of PCR product from the pre-amplification step were purified using 50 μL of Ampure XP beads (Beckman Coulter), resuspended ...
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bioRxiv - Developmental Biology 2021Quote: ... PCRs were monitored by qPCR to avoid over-ampliciation and following every PCR reaction the samples were purified using SPRI beads (Beckman Coulter) and libraries were sequenced at 1:10 proportion of the transcriptome library on Illumina’s NovaSeq.
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bioRxiv - Genomics 2020Quote: ... We carried out the first round of PCR for 3 cycles and purified the reactions with Ampure XP beads (Beckman-Coulter). We then carried out a second round of PCR with primers targeting the P5 and P7 sequences only ...
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bioRxiv - Microbiology 2021Quote: ... with a final extension at 72 °C for 10 min PCR products were purified using Agencourt AMPure XP beads (Beckman Coulter) ratio 0.6× ...
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bioRxiv - Microbiology 2020Quote: ... We pooled the triplicate PCR products and the 24 μL of final volume were purified by adding 44 μL of AMPure XP beads (Beckman Coulter), mixing with pipette followed by a 5 minute incubation ...
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bioRxiv - Genomics 2020Quote: ... These pooled single-cell libraries were used in an AMpure XP Bead-based Dual Bead Cleanup and Size Selection reaction (Agencourt AMPure XP PCR Purification modified Instructions for Use, Beckman Coulter). In both bead cleanup reactions ...
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bioRxiv - Molecular Biology 2023Quote: ... Size selection purifications following the ligation and amplification PCR steps were performed with 0.9× reaction volumes of Agencourt AMPure XP beads (Beckman Coulter A63880). Purified libraries were combined as an 8-sample equimolar pool containing a combination of indexes from 1–12 and sequenced on an Illumina NextSeq on an Illumina NextSeq 2000 on a P2 flow cell (paired-end 75-bp reads).
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bioRxiv - Genomics 2023Quote: ... The PCR amplification is followed by a bead clean up step with 1X sample-to-bead ratio AMPure XP beads (Beckman Coulter), binding at room temperature for 5 min ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The PCR amplicons of the samples were then pooled after a purification and concentration equalization process with the AMPureXP Kit (Beckman Coulter). The libraries were processed in an Illumina MiSeq v2 (500 cycles and paired-end).
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bioRxiv - Genomics 2023Quote: ... Full length transcripts were selected by PCR amplification using the LongAmp Taq Master Mix and the product was purified with AMPure XP beads (Beckman Coulter). An aliquot of 1 µL of Rapid Adapter was added to the amplified cDNA library ...