Labshake search
Citations for Beckman :
801 - 850 of 1915 citations for Trisodium dichloroanthra 2 1 9 mna naphth 2 3 h acridine 5 10 15 triyl tris sulfate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... This crude mitochondrial pellet was further purified on a discontinuous percoll gradient (buffer composition 15/23/40% sucrose) by centrifugation (40,000 x g, 1hr, 4°C) in an SW41 rotor (Beckman Coulter) and enriched mitochondria were collected from the 23-40% interface (adapted from [76]) ...
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bioRxiv - Biophysics 2023Quote: ... Reactions were centrifuged at 400,000 g at 20 °C for 15 min in polycarbonate centrifuge tubes (Beckman Coulter Inc. USA). The pellets were resuspended in 40 μl reaction buffer ...
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bioRxiv - Genomics 2023Quote: ... The ribosomes were further purified by centrifugation at 59,727 ×g (15 hours, 4 °C, SW 32.1 Ti rotor, Optima™ L-100 XP, Beckman Coulter) in a linear (15 – 30 % ...
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bioRxiv - Neuroscience 2023Quote: ... the crude lysate was subjected to iodixanol gradient (15%, 25%, 40% and 60%) ultracentrifugation using a Type 70Ti rotor (Beckman) at 490000 × g ...
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bioRxiv - Cell Biology 2022Quote: ... 40 μl of the mixture was then transferred to an ultracentrifuge tube and centrifuged at around 100,000 g for 15 minutes at room temperature using an airfuge (Beckman Coulter). After the centrifugation ...
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Faa1 membrane binding drives positive feedback in autophagosome biogenesis via fatty acid activationbioRxiv - Biochemistry 2023Quote: ... The reaction was centrifuged at 68 000 rpm for 15 min at 22 °C (Beckman-Coulter Optima MAX, TLA100 rotor). The supernatant and pellet were loaded separately on an SDS/polyacrylamide gel in equal amounts ...
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bioRxiv - Neuroscience 2022Quote: ... AAV was purified from the resulting lysate using an iodixanol step gradient containing 15, 25, 40 and 60% iodixanol (Batool et al., 2021) in optiseal tubes (Beckman) followed by centrifugation at 350,000 xg using a Type 70 Ti ultracentrifuge rotor (Beckman) ...
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bioRxiv - Pathology 2023Quote: ... Ligated cDNAs were amplified following 15 PCR cycles and PCR products were purified using AMPure XP Beads (Beckman Coulter Genomics). Libraries were validated using a Bioanalyzer on a DNA1000 chip (Agilent ...
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bioRxiv - Microbiology 2023Quote: ... followed by downstream RNA extraction procedures as per manufacturer’s instructions including a DNase treatment step at 37°C for 15 min (Beckman Coulter).
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bioRxiv - Microbiology 2024Quote: ... About 0.7 ml of combined pellet suspension from all ultracentrifuge tubes were loaded on 15–50% linear gradient of sucrose made in PBS and spun in SW41 (Beckman) rotor at 80,000 × g for 1 h at 4 °C ...
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bioRxiv - Cell Biology 2024Quote: ... and the post-nuclear supernatant was then centrifuged at 49,000 RPM for 15 min in an TLA-55 ultracentrifuge (Beckman Coulter). The supernatant (cytosol fraction ...
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bioRxiv - Cell Biology 2024Quote: ... 0.5 ml of the dialysate was overlayed onto a 5-30% sucrose gradient made in dialysis buffer and resolved by centrifuging the gradients overnight (15-16h) at 168,000xg in a SW40 rotor (Beckman Coulter). The gradient was manually fractionated into 0.5 ml fractions and a small sample from each fraction was run on an SDS-PAGE gel and stained with Sypro Ruby to visualize protein bands ...
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bioRxiv - Cell Biology 2021Quote: ... final cell counts were measured using a Multisizer 3 Coulter Counter (Beckman Coulter). The following formula was used to calculate proliferation rate:
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bioRxiv - Cell Biology 2020Quote: ... cells were washed 3 times and analyzed with MoFlo (Beckman, Brea, CA, USA) and associated software ...
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bioRxiv - Cell Biology 2020Quote: Size of HSCs was determined using a Multisizer-3 Coulter Counter (Beckman Coulter) or microscopy ...
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bioRxiv - Immunology 2024Quote: ... and then ultracentrifuged for 3 hours with a SW32 Ti rotor (Beckman Coulter) at 100,000 x g at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... cells were counted with an electronic particle counter (Multisizer 3 counter; Beckman Coulter) and 250 mL of SM ...
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bioRxiv - Immunology 2024Quote: Cell counting: single-cell suspensions were counted using a Multisizer 3 (Beckman Coulter) set to detect nuclei between 3.5-10um ...
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bioRxiv - Immunology 2021Quote: Immune cell types in Cohort 1 were measured from whole blood by 10-color flow cytometry with Navios flow cytometer (Beckman Coulter, CA, USA). Staining and gating strategies were previously described in detail by Aguirre-Gamboa et al ...
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bioRxiv - Biochemistry 2021Quote: ... 10 U/mL SuperASE-In and was ultracentrifuged at 100,000 × g for for 16 h at 4 °C in a SW Ti41 rotor (Beckman Coulter). After removing the supernatant ...
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bioRxiv - Biochemistry 2022Quote: ... Viral particles were pelleted at 30,000 rpm for 1.5 h at 25°C in an Optima XPN ultracentrifuge using an SW 32 Ti rotor (Beckman Coulter), resuspended in 500 μL DMEM supplemented with 10% FBS ...
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bioRxiv - Neuroscience 2021Quote: ... PMCA products were centrifuged at 45,000 rpm for 2.5 h at 4 °C (Optima™ Max Ultracentrifuge, Beckman Coulter, USA), the supernatants discarded and the pellets resuspended in an equal volume of PBS.
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bioRxiv - Molecular Biology 2021Quote: ... The samples were centrifuged at 34,000 rpm at 4°C for 16 h using an SW60 Ti rotor (Beckman Coulter). Subsequently ...
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bioRxiv - Molecular Biology 2020Quote: ... Gradients were centrifuged at 39 000 rpm for 2.5 h at 4°C in an Optima L-100XP ultracentrifuge (Beckman– Coulter) using the SW41Ti rotor with brake ...
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bioRxiv - Microbiology 2021Quote: ... Viral bands were pooled and re-precipitated by centrifugation in PBS(-) at 120k × g for 1.5 h in an SW55 rotor (Beckman Coulter) at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... the sample was centrifuged for 16 h at 4°C and 100,000 rcf using a type 70 Ti rotor (Beckman Coulter). The pellet was gently washed with 500 μl of storage buffer (lysis buffer C + 10% (v/v ...
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bioRxiv - Microbiology 2021Quote: ... The density gradient centrifugation was performed at 141,000 g and 4 °C for 20 h in a SW28 swing rotor (Beckman Coulter). A total of 18 fractions were collected starting from the top.
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bioRxiv - Cell Biology 2020Quote: ... The supernatant of MNase treated chromatin were overlaid onto the sucrose gradient and spun at 32000 rpm (124,436 rcf) for 13 h at 4 °C using SW55Ti rotor (Beckman Coulter) in Beckman Optima L80.
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bioRxiv - Cell Biology 2020Quote: ... 1 mM EGTA) and spun at 32000 rpm (124,436 rcf) and 4 °C for 13 h using SW55Ti rotor (Beckman Coulter) in Beckman Optima L80 ...
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bioRxiv - Molecular Biology 2021Quote: ... lysates were spun for 24 h at 4°C at 50000 rpm in a Type 70 Ti rotor (Beckman Coulter) through a 40 % (w/v ...
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bioRxiv - Immunology 2020Quote: ... all CCMV CP preparations were purified two times using an ultracentrifuge and 30% sucrose cushion – first with additional 0.5% Triton X-100 and the second time without Triton X-100 (4 h, 50 000 rmp, 4°C; Type 70Ti rotor, Beckman) and the pellet was then dissolved in buffer A ...
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bioRxiv - Biochemistry 2020Quote: ... incubated under constant shaking at 37°C taken at various time points (24, 72, 168 h) were centrifuged (TL-100, Beckman) at 50k RPM (100,000 ×g ...
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bioRxiv - Systems Biology 2023Quote: ... Gradients were centrifuged at 4°C for 17 h at 100,000 g using an L7 ultracentrifuge with an SW28 swing out rotor (Beckman Coulter). Fractions (F ...
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bioRxiv - Cell Biology 2022Quote: ... 4–40% sucrose gradient prepared in lysis buffer and centrifuged at 150,000 × g for 4 or 16 h at 4°C in a MLS-50 rotor (Beckman Coulter). Thereafter ...
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bioRxiv - Plant Biology 2023Quote: ... Approximately 250 µl of crude EV sample resuspended in 20 mM Tris-HCl pH 6.0 was slowly dispensed on top of the column and centrifuged at 100,000 x g for 17 h at 4 ⁰C (SW 41 Ti rotor, Beckman Coulter; OptimaTM L-90K, Beckman Coulter). After the ultracentrifugation ...
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bioRxiv - Cell Biology 2022Quote: ... to obtain a high percentage of transfected cells 24 h post-transfection GFP expressing cells were FACS sorted by MoFlo Astrios (Beckman). Following sorting total RNA was prepared ...
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bioRxiv - Biochemistry 2024Quote: ... The protein samples were carefully applied to the gradient and centrifuged for 16 h at 130,000 g at 4 °C (Optima XPN-80 Ultracentrifugation from Beckman Coulter). 200 µl fractions were taken from the top of the gradient and the glutaraldehyde quenched with 20 mM Tris pH 7.5 ...
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bioRxiv - Cell Biology 2023Quote: ... The gradient was centrifuged at 4 °C and 100,000 ˗ g for 18 h (Optima L-80 XP Ultracentrifuge with Swing Rotor (Beckman Coulter)) ...
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bioRxiv - Microbiology 2023Quote: ... Medium was cleared from EVs by ultracentrifugation at 100,000 ×g for 18 h at 4°C in a LE-80 ultracentrifuge (Beckman Coulter) with a 60Ti rotor [13] ...
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bioRxiv - Neuroscience 2023Quote: ... the samples were filtered with a membrane pore size of 0.2 μm and centrifuged at 120,000 × g for 4 h at 4 °C in polycarbonate bottle assemblies (Beckman coulter, 355603) in a 70.1 Ti fixed angle rotor (Beckman coulter ...
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bioRxiv - Biophysics 2023Quote: ... Centrifugation was performed at 154,300 × g for 24 h at 4◦C (SW 41 Ti swinging-bucket rotor, Beckman Coulter). The separated bands were collected dropwise from the bottom of the tube ...
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bioRxiv - Neuroscience 2023Quote: ... The gradients were centrifuged for 18 h at 100,000 xg at 4 °C in a SW 40 rotor (Beckman Coulter). 500 µl fractions were collected from the top of the ultracentrifuge tubes and analyzed by WB analysis.
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bioRxiv - Biophysics 2023Quote: ... The isolated crude membrane extract was loaded on the continuous 20-70 % sucrose density gradient in 20 mM NaH2PO4/Na2HPO4 pH 7.4 and 100 mM NaCl prepared by the Gradient Station (Biocomp) and centrifuged for 16 h at 30.000 rpm (rotor SW 40 Ti, Beckman Coulter). The gradients were collected with the Gradient Station ...
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bioRxiv - Microbiology 2024Quote: ... sucrose gradients prepared with buffer C and centrifuged for 16 h at 71,935 g at 4 °C in a SW28 rotor (Beckman Coulter). Gradients were fractionated on a Biocomp Piston Gradient Fractionator (BioComp Instruments ...
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bioRxiv - Microbiology 2024Quote: ... Samples were added to the top of a 60%-30%-10% sucrose/PBS gradient and centrifuged at 16,000 x g for 4.5 h at 4°C with an SW32 Ti rotor (Beckman, Brea, CA). Virus bands at the 60%-30% sucrose interface were collected via tube side puncture ...
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bioRxiv - Microbiology 2024Quote: ... and fractionated by centrifugation at 32 000 rpm (130 000 × g) for 17 h using an SW-41 rotor (Beckman). The gradient was divided into 20 fractions and proteins from each fraction were resolved on SDS-PAGE and detected by western blotting.
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bioRxiv - Molecular Biology 2024Quote: ... Transfected cells recovered for 72 h before sorting for single cell clones on a MoFlo Astrios Cell Sorter (Beckman Coulter). Single cell clones were validated by genotyping and immunoblotting.
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bioRxiv - Cell Biology 2021Quote: ... for 5 minutes at 4°C with TLA100 rotor (Beckman). 100 µg cell lysate was brought to a final volume of 250 µl BRB80 supplemented with 1 mM GTP ...
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bioRxiv - Molecular Biology 2023Quote: ... 5 mM MgCl2) and spun in an SW41Ti rotor (Beckman) for 20 hours at 30,900 rpm ...
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bioRxiv - Neuroscience 2022Quote: ... 10-color Gallios flow cytometer (Beckman-Coulter).