Labshake search
Citations for Beckman :
801 - 850 of 1121 citations for CTLA 4 Mouse HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... The diluted extract was then centrifuged at 31,200 rpm for 30 minutes at 4°C in a Type 70 Ti rotor (Beckman Coulter). The supernatant was filtered through a 0.45 µm syringe filter (Millex-GP PES membrane (Millipore SLHP033RS ...
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bioRxiv - Biochemistry 2023Quote: ... The lysate was centrifuged at 40,000 rpm for 60 min at 4°C (XPN-100 ultracentrifuge, Type 45 Ti rotor, Beckman, USA). The supernatant was filtered through a 0.45 μm filter (Pall ...
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bioRxiv - Bioengineering 2023Quote: ... For performing cryogenic transmission electron microscopic imaging EVs were centrifuged and washed in PBS at 100,000 x g for 3 hours at 4°C in a SW41 Ti rotor (Beckman Coulter).
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bioRxiv - Neuroscience 2023Quote: Monomeric wild-type α-syn was ultracentrifuged at 90,000 RCF for 1 hour at 4 °C to remove any amorphous aggregates (Beckman Coulter Optima Max-XP with MLA-130 rotor) ...
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bioRxiv - Microbiology 2023Quote: ... Media was cleared from EVs by ultracentrifugation at 100,000×g for 18 h at 4 °C in a LE-80 ultracentrifuge (Beckman Coulter) with a 60Ti rotor ...
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bioRxiv - Cell Biology 2023Quote: ... The concentrated CFS was then ultracentrifuged at 100,000 ˗ g and 4 °C for 3 h (Optima L-80 XP Ultracentrifuge with Type 70.1 Ti Fixed-Angle Titanium Rotor (Beckman Coulter)) ...
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bioRxiv - Cell Biology 2023Quote: ... the fifth virus was freeze-thawed three times and ultracentrifuged at 23 k rpm at 4 °C for 1.5 hours in the Super-centrifuge Optima XE-90 Ultracentrifuge (Beckman Coulter) using Ultra-Clear Centrifuge Tubes (Beckman Coulter) ...
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bioRxiv - Bioengineering 2023Quote: ... The final supernatant was centrifuged at 100,000g at 4°C for 70 min using an ultracentrifuge (Optima MAX-XP Tabletop Ultracentrifuge, Beckman Coulter). The pellet was either used immediately or stored dry at −80°C ...
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bioRxiv - Biochemistry 2023Quote: ... and the mixtures were then layered under a 20-40% iodixanol discontinuous gradient before to be centrifuged at 260,000 x g for 16h at 4°C using an SW41 Ti rotor (Beckman Coulter). After spinning ...
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bioRxiv - Biophysics 2023Quote: ... Centrifugation was performed at 154,300 × g for 24 h at 4◦C (SW 41 Ti swinging-bucket rotor, Beckman Coulter). The separated bands were collected dropwise from the bottom of the tube ...
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bioRxiv - Molecular Biology 2024Quote: ... Lysates were cleared by centrifugation for 1 h at 45,000 rpm at 4°C (Optima XPN-80 Ultracentrifuge with 70 Ti, Beckman Coulter). HTP-tagged proteins were immunoprecipitated with 600 µl pre-washed IgG-sepharose 6 fast flow affinity resin (GE Healthcare ...
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bioRxiv - Cell Biology 2024Quote: ... Gradients were centrifuged at 36,000 rpm for 2 h at 4 °C in an Optima L-100XP ultracentrifuge (Beckman–Coulter). Following centrifugation ...
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bioRxiv - Microbiology 2024Quote: ... The resulting gradient was then subjected to centrifugation for 16 hours at 150,000 x g and 4 °C (SW55 Ti rotor, Beckman-Coulter). Sample banding was visually observed at the interface of the 30% and 20% fractions (Supporting Information ...
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bioRxiv - Neuroscience 2024Quote: ... Digested samples were ultracentrifuged at 100,000x g for 1 h at 4 °C using a TLA-55 rotor (Beckman Coulter) to concentrate the insoluble ...
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bioRxiv - Cell Biology 2024Quote: ... The step gradients were centrifuged for 5 h at 132.000 x g at 4 °C (SW60 Beckman and Coulter, # 335649). Subsequently ...
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bioRxiv - Cell Biology 2024Quote: ... The same amounts of proteins were loaded on a double sucrose cushion (20% and 30% sucrose) and centrifuged at 190,000 g for 2 h at 4 °C in an Optima L-100XP ultracentrifuge (Beckman–Coulter). Extracts were incubated with pre-washed anti-Flag beads (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... The samples were sedimented by velocity centrifugation at 36,000 x rpm for 2 h at 4°C using SW40Ti rotor in Optima L-80XP ultracentrifuge (Beckman Coulter). Absorbance at 254 nm was measured from lower to higher sucrose gradients using an ISCO gradient fractionation system and the optical density at 254 nm was continuously recorded with a Foxy JR Fractionator (Teledyne ISCO).
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bioRxiv - Molecular Biology 2024Quote: ... rpm at 4 ºC for 40 min using a JS 5.3 rotor in an Avanti J-26S centrifuge (Beckman Coulter). Pellets containing fixed chromosomes were resuspended with 10 mL of buffer SC ...
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bioRxiv - Molecular Biology 2024Quote: ... rpm at 4 ºC for 40 min using a JS 5.3 rotor in an Avanti J-26S centrifuge (Beckman Coulter). The chromosomes were collected from the bottom of the centrifuge tube and resuspended with buffer SC ...
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bioRxiv - Molecular Biology 2024Quote: ... The insoluble pellets were washed by resuspending them in 50 ml of buffer E and re-collecting them through centrifugation at 20,000 rpm at 4 ºC for 30 min using a 45Ti rotor in Optima L80 (Beckman Coulter). The washed insoluble pellets were resuspended in 8 mL of 6 M guanidine HCl (pH 1.5 ...
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bioRxiv - Molecular Biology 2024Quote: ... The denatured proteins were collected by centrifugation at 20,000 rpm at 4 ºC for 30 min using a 45Ti rotor in Optima L80 (Beckman Coulter). Protein concentrations in soluble fractions were estimated based on the absorbance at 260 nm ...
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bioRxiv - Molecular Biology 2024Quote: ... the rest was loaded on top of sucrose gradient and ultracentrifuged (30000 rpm, 4°C, 18h, stop DECEL, slow, SW40/1Ti swinging bucket rotor, Optime L-90K, Beckman). 24 fractions were taken from top to bottom of the tube as 500 µL aliquots and precipitated by incubation with 50 µL of 0.15% sodium deoxycholate for 10 min at RT ...
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bioRxiv - Molecular Biology 2024Quote: ... aggregated proteins were removed by centrifugation at 20,000 rpm at 4 ºC for 30 min using a 45Ti rotor in Optima L80 (Beckman Coulter). The supernatant was mixed with 62.7 g of solid ammonium sulfate and stirred overnight at 4 ºC ...
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bioRxiv - Molecular Biology 2024Quote: ... the mixture was spun at 13,894 rpm (16,000 rcf) at 4 ºC for 10 min using the SX241.5 rotor in an Allegron X-30R centrifuge (Beckman Coulter). The beads that accumulated at the bottom of the tube were resuspended in 200 µL of EM buffer A ...
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bioRxiv - Molecular Biology 2024Quote: ... they were collected by centrifugation at 13,894 rpm (16,000 rcf) at 4 ºC for 20 min using the SX241.5 rotor in an Allegron X-30R centrifuge (Beckman Coulter). Subsequently ...
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bioRxiv - Molecular Biology 2024Quote: ... and spun at 32,000 rpm (max 124,436 rcf) and 4 ºC for 13 h using an SW55Ti rotor in an Optima L80 centrifuge (Beckman Coulter). The samples were fractionated from the top of the sucrose gradient ...
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bioRxiv - Molecular Biology 2024Quote: ... Insoluble fractions were collected by centrifugation at 20,000 rpm at 4 ºC for 30 min using a 45Ti rotor in Optima L80 (Beckman Coulter). The insoluble pellets were washed by resuspending them in 50 ml of buffer E and re-collecting them through centrifugation at 20,000 rpm at 4 ºC for 30 min using a 45Ti rotor in Optima L80 (Beckman Coulter) ...
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bioRxiv - Molecular Biology 2024Quote: ... The soluble fractions released by MNase were isolated by taking supernatants after centrifugation at 13,894 rpm (16,000 rcf) at 4 ºC for 30 min using an SX241.5 rotor in an Allegron X-30R centrifuge (Beckman Coulter). The supernatants were collected and layered onto a 10-22 % linear sucrose gradient solution with buffer SG (15 mM HEPES-KOH [pH 7.4] ...
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bioRxiv - Molecular Biology 2024Quote: ... Insolubilized proteins were removed with centrifugation at 20,000 rpm at 4 ºC for 30 min using a 45Ti rotor in Optima L80 (Beckman Coulter). The supernatant was loaded into the HisTrap HP column (Cytiva) ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were centrifuged at 30,000 xg for 45 min at 4 °C using a SW41 Ti swinging bucket rotor (Beckman Coulter) before the supernatant was decanted ...
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bioRxiv - Neuroscience 2024Quote: ... The homogenate was centrifuged (45 min, 16 000 rpm, 4°C, JLA 16.250 rotor, Beckman-Coulter Centrifuge Avanti J25-01) and the supernatant subjected to Ni-NTA affinity purification (HIS-Select Nickel Affinity Gel (Sigma Aldrich)) ...
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bioRxiv - Neuroscience 2024Quote: ... The supernatant was ultracentrifuged at 80,000 × g for 1 h at 4°C using a type 50 Ti rotor ultracentrifuge (Beckman Coulter). The supernatant ...
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bioRxiv - Biochemistry 2024Quote: ... The homogenate was clarified by centrifugation for 30 min at 70,560 × g at 4°C in an Optima XPN Ultracentrifuge (Beckman Coulter) using a Ti-45 rotor ...
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bioRxiv - Genomics 2023Quote: ... 1x protease inhibitor cocktail EDTA-free) by spinning at 38,000 rpm at 4 °C in a SW-41 Ti rotor (Beckman Coulter). Monosome pellets were resuspended in 400 µL lysis buffer and rocked overnight ...
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bioRxiv - Biochemistry 2024Quote: ... The protein samples were carefully applied to the gradient and centrifuged for 16 h at 130,000 g at 4 °C (Optima XPN-80 Ultracentrifugation from Beckman Coulter). 200 µl fractions were taken from the top of the gradient and the glutaraldehyde quenched with 20 mM Tris pH 7.5 ...
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bioRxiv - Biochemistry 2024Quote: ... The homogenate was clarified by centrifugation for 30 min at 70,560 × g at 4°C in an Optima XPN Ultracentrifuge (Beckman Coulter) using a Ti-45 rotor ...
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bioRxiv - Biophysics 2024Quote: ... and subjected to ultracentrifugation at 384,492□g for 45□min at 4□°C (Beckman Coulter Optima MAX, TLA-100 rotor), stored at −80 °C after flash-frozen with liquid nitrogen for crystallization trials ...
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bioRxiv - Microbiology 2021Quote: ... and stained intracellularly using PE-conjugated mouse anti-p24 mAb (clone KC57; Beckman Coulter; 1:100 dilution). The percentage of transfected or infected cells (p24+ ...
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bioRxiv - Plant Biology 2021Quote: ... the samples were centrifuged at 35 000 RPM for 18 hours at 4°C in a 70Ti rotor and L8-M ultracentrifuge (Beckman Coulter). The ribosome pellets were then gently washed with RNase-free water and resuspended in 300 μl of resuspension buffer (100 mM Tris·HCl (pH 8.0) ...
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bioRxiv - Cell Biology 2019Quote: ... by ultracentrifugation at 150,000 × g for 12 h at 4°C using an Optima L-100XP centrifuge equipped with a SW55Ti rotor (Beckman Coulter). Eleven fractions were collected from the top of the sucrose gradient.
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bioRxiv - Evolutionary Biology 2021Quote: ... using a Beckman Coulter TLA100 rotor at 70,000 rpm at 4 C for 2 hours in a 13 mm x 51 mm polycarbonate ultracentrifuge tube (Beckman Coulter 349622). The sucrose solution was removed from the tube ...
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bioRxiv - Molecular Biology 2021Quote: ... The gradient was ultracentrifuged at 55,000 rpm for 55 min at 4 °C with max acceleration and slow deceleration using an Optima LE-80K Ultracentrifuge and SW55Ti rotor (Beckman Coulter). The sucrose gradient was fractionated according to the number of associated ribosomes as determined by the profile of the absorbance at 254 nm using a Density Gradient Fractionation System (Brandel ...
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bioRxiv - Neuroscience 2021Quote: ... This was incubated for 1 hour at room temperature while rotating before centrifugation at 100,000 x g for 90 minutes at 4°C using the Optima™ MAX-XP Ultracentrifuge (Beckman Coulter). The supernatant was then collected (sarkosyl-soluble fraction) ...
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bioRxiv - Molecular Biology 2021Quote: ... pombe cells were pelleted down for 10 min at 5000 rpm at 4°C (Rotor: F10-6X500, FiberLite Beckman J2-MC). The pellet was washed with buffer 1 (HEPES- 20 mM ...
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bioRxiv - Bioengineering 2022Quote: ... The 60-70 mL were then divided equally into two 50-mL centrifuge tubes and spun down outside the anaerobic chamber at 4 °C and 3500 rpm for 10 min using an Allegra 64R ultracentrifuge (Beckman Coulter). Next ...
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bioRxiv - Bioengineering 2022Quote: ... Each 250 mL culture was divided equally into six 50-mL centrifuge tubes and spun down at 4 °C and 3500 rpm for 10 min using an Allegra 64R centrifuge (Beckman Coulter). The supernatant was discarded and 6 mL of ice-cold 10% glycerol was used to wash and combine the six cell pellets into one suspension ...
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bioRxiv - Genomics 2020Quote: ... Lentiviral supernatant was collected at 48 and 72hrs post-transfection and subsequently concentrated by ultracentrifugation (24,000 rpm, 4°C, 2hrs) (Beckman Coulter SW32).
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bioRxiv - Biophysics 2019Quote: AUC-SV analyses were performed in XL-A analytical ultracentrifuge equipped with a 4-hole An-60 Ti rotor and 12-mm double-sector charcoal-filled epon centerpieces (Beckman Coulter). Sedimentation velocity measurements using absorbance optics of reference buffer and samples in 20 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Cell Biology 2019Quote: Viability of unlabeled and ferumoxytol-labeled murine MSCs (n=4) and human MSCs (n=3) was assessed by Trypan blue dye exclusion using ViCell (Beckman Coulter).
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bioRxiv - Molecular Biology 2019Quote: ... The cleared supernatant was layered over 15-40% sucrose gradients in disassociation buffer and spun 28000 rpm for 16 hours at 4°C in a SW-32 rotor (Beckman-Coulter). Gradients were fractionated with an ISCO fractionation system and 30S + 50S peaks were combined ...