Labshake search
Citations for Beckman :
801 - 850 of 1322 citations for 2 4 Dimethyl 4' 4 methylpiperazinomethyl benzophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... at 4°C until equilibrium was reached using an An Ti 60 rotor in an XL-I analytical ultracentrifuge (Beckman), run using ProteomeLab XL-A/XL-I (version 6.2) ...
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bioRxiv - Molecular Biology 2023Quote: ... for 45[min at 25°C and then subjected to a sucrose cushion ultracentrifugation at 100,000[rpm for 1[h at 4°C with Optima MAX-TL ultracentrifuge and TLA-110 rotor (Beckman Coulter). The RNA fragments in the resulting pellet were recovered using TRIzol reagent (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... and centrifuged at 100,000 × g for 1 h at 4 °C using a TLA-55 rotor in an Optima TLX Ultracentrifuge (Beckman Coulter). The first supernatant ...
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bioRxiv - Genomics 2023Quote: ... the supernatant was cleared by two rounds of centrifugation at 20,000 ×g (10 min, 4 °C, JA-20 rotor, Avanti J-26 XP, Beckman Coulter) followed by centrifugation at 31,900 ×g (10 min ...
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bioRxiv - Genomics 2023Quote: ... Precipitated ribosomes were pelleted at 20,000 ×g (10 min, 4 °C, JA-20 rotor, Avanti J-26 XP, Beckman Coulter). To remove residual supernatant the pellet was re-centrifuged for 5 min at 20,000 ×g ...
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bioRxiv - Genomics 2023Quote: ... The suspension was incubated on ice for 5 min and centrifuged at 20,000 ×g (10 min, 4 °C, JA-20 rotor, Avanti J-26 XP, Beckman Coulter). The pellet was re-centrifuged for 5 min and both supernatants were combined into a new tube ...
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bioRxiv - Cancer Biology 2023Quote: ... clarified by centrifugation at 20,000 rpm (48,385 x g) for 30 min in a JA25.50 rotor at 4 °C (Beckman AvantiTM J-25), filtered through a 0.8/0.2 AcroPak Filter Unit (Pall) ...
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bioRxiv - Genomics 2023Quote: ... The ribosomes were further purified by centrifugation at 59,727 ×g (15 hours, 4 °C, SW 32.1 Ti rotor, Optima™ L-100 XP, Beckman Coulter) in a linear (15 – 30 % ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were seeded into 6-well plates at a concentration of 4×105 cells per well and counted cumulatively with a Coulter counter (Beckman) every three days for two weeks ...
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bioRxiv - Plant Biology 2023Quote: ... The cAW was collected by spinning at 700 x g for 20 min at 4 ⁰C (JA-14 rotor, Beckman Coulter ...
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bioRxiv - Biochemistry 2023Quote: ... The gradient was centrifuged for 2.30 hours at 4 °C and 111,000 ×g in a swing-out rotor (Beckman- Coulter, SW41). The brownish lysosome-enriched fraction (∼ 1ml ...
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bioRxiv - Cancer Biology 2023Quote: ... Filtered supernatants were concentrated by ultracentrifugation at 22.000 rpm for 2h at 4°C with OptimaTM L-90K ultracentrifuge (Beckman Coulter) and stored at −80°C (never refrozen).
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bioRxiv - Cell Biology 2023Quote: ... The gradient was centrifuged at 4 °C and 100,000 ˗ g for 18 h (Optima L-80 XP Ultracentrifuge with Swing Rotor (Beckman Coulter)) ...
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bioRxiv - Cell Biology 2023Quote: ... Equilibrated tubes were ultra-centrifuged at 100,000xg for 1h at 4°C using a swinging-bucket rotor (SW 32Ti, Beckman Coulter, 369650; Optima XE, Beckman Coulter). Phagosome-containing beads float at the interface between the 10% and 25% layers ...
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bioRxiv - Biophysics 2023Quote: ... The lysate was clarified by centrifugation at 40,000 rpm for 35 min at 4°C (Type 45 Ti Rotor, Beckman Coulter). The resulting supernatant was mixed with 1ml pre-washed IgG Sepharose 6 FastFlow beads (Cytiva ...
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bioRxiv - Cell Biology 2023Quote: ... and pelleted at 100,000xg for 20 min at 4 °C using fixed angle rotor (TLA-100 rotor, Beckman Coulter, 349481) in a benchtop ultracentrifuge (OptimaTL ...
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bioRxiv - Neuroscience 2023Quote: ... and samples were centrifuged at 24,000 rpm at 4°C for 1h in a SW41Ti Swinging-Bucket Rotor (Beckman Coulter). After discarding the supernatant ...
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bioRxiv - Immunology 2023Quote: ... virus solutions were clarified of debris by centrifugation at 10,000 rpm at 4°C for 30 minutes in a chilled SW32Ti rotor (Beckman Coulter), followed by pelleting through a 30% cold sucrose solution at 25,000 rpm for 90 minutes ...
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bioRxiv - Microbiology 2023Quote: ... Cells were washed twice with 200µl BD 1X Perm/Wash solution and stained 1h at 4°C with anti-HIV-1 p24 (RD1/PE, Beckman Coulter #6604667 ...
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bioRxiv - Microbiology 2023Quote: ... Medium was cleared from EVs by ultracentrifugation at 100,000 ×g for 18 h at 4°C in a LE-80 ultracentrifuge (Beckman Coulter) with a 60Ti rotor [13] ...
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Centriole and transition zone structures in photoreceptor cilia revealed by cryo-electron tomographybioRxiv - Cell Biology 2023Quote: ... OptiPrep step-gradient and centrifuged for 60 min at 19,210 × g at 4 °C in a TLS-55 rotor (Beckman Coulter). The ROS band was collected with an 18G needle ...
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bioRxiv - Cell Biology 2023Quote: ... Small EVs were isolated by ultracentrifugation at 120,000 x g and 4°C for 120 min using Optima XE-90 equipment with a SW32Ti rotor (Beckman Coulter). Supernatants were discarded using a pipette ...
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bioRxiv - Biochemistry 2023Quote: ... The sucrose density gradient was centrifuged at 50,000 rpm for 3 hours at 4°C in a TLS-55 swinging bucket rotor (Beckman Coulter) with minimum acceleration up to 5,000 RPM and deceleration with no break ...
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bioRxiv - Biochemistry 2023Quote: ... The diluted extract was then centrifuged at 31,200 rpm for 30 minutes at 4°C in a Type 70 Ti rotor (Beckman Coulter). The supernatant was filtered through a 0.45 µm syringe filter (Millex-GP PES membrane (Millipore SLHP033RS ...
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bioRxiv - Biochemistry 2023Quote: ... The lysate was centrifuged at 40,000 rpm for 60 min at 4°C (XPN-100 ultracentrifuge, Type 45 Ti rotor, Beckman, USA). The supernatant was filtered through a 0.45 μm filter (Pall ...
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bioRxiv - Bioengineering 2023Quote: ... For performing cryogenic transmission electron microscopic imaging EVs were centrifuged and washed in PBS at 100,000 x g for 3 hours at 4°C in a SW41 Ti rotor (Beckman Coulter).
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bioRxiv - Neuroscience 2023Quote: Monomeric wild-type α-syn was ultracentrifuged at 90,000 RCF for 1 hour at 4 °C to remove any amorphous aggregates (Beckman Coulter Optima Max-XP with MLA-130 rotor) ...
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bioRxiv - Cell Biology 2023Quote: ... The concentrated CFS was then ultracentrifuged at 100,000 ˗ g and 4 °C for 3 h (Optima L-80 XP Ultracentrifuge with Type 70.1 Ti Fixed-Angle Titanium Rotor (Beckman Coulter)) ...
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bioRxiv - Neuroscience 2024Quote: ... and samples were centrifuged at 24,000 rpm at 4°C for 1h in a SW41Ti Swinging-Bucket Rotor (Beckman Coulter). After discarding the supernatant ...
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bioRxiv - Microbiology 2024Quote: ... 20 mL of supernatant was gently layered on top of 10mL of 20% sucrose dissolved in PBS (4°C, sterile filtered) in an ultra-clear 1×3.5′ centrifuge tube (Beckman 344058). The tubes were spun down in a SW28 rotor (Beckman ...
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bioRxiv - Biochemistry 2024Quote: ... Lysates were clarified by ultracentrifugation at 4℃ at 184,000 x (g) in a 70Ti rotor for 50 minutes (Beckman Coulter). After affinity chromatography with StrepTactin SuperFlow resin (IBA) ...
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bioRxiv - Molecular Biology 2024Quote: ... and EVs were pelleted by ultracentrifugation for 60 minutes at 100,000 x g at 4 ° C in an SW40.Ti rotor (Beckman Coulter). After centrifugation ...
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bioRxiv - Biochemistry 2024Quote: ... A crude ribosome pellet was obtained by centrifugation for 2h at 200,000 × g at 4 °C (70Ti rotor, Beckman Coulter) and solubilized in HMA6/30 for 16h at 4°C ...
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bioRxiv - Evolutionary Biology 2024Quote: ... EVs were purified from concentrated HES by ultracentrifugation at 100,000 x g for 90 mins at 4 °C in polyallomer tubes (Beckman Coulter) in a SW40 rotor (Beckman Coulter) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and pelleted each time by ultracentrifugation at 100,000 x g for 70 mins at 4 °C in polyallomer tubes (Beckman Coulter) in a SW40 rotor (Beckman Coulter) ...
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bioRxiv - Plant Biology 2024Quote: ... and centrifuged at 10,000 x g for 30 min at 4°C using a TLA100.3 fixed angle rotor and an Optima TLX Ultracentrifuge (Beckman Coulter).
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bioRxiv - Cell Biology 2024Quote: ... The samples were then centrifuged for 1 h at 100,000 g and 4°C in a TLS 55 rotor (Beckman-Coulter) with the appropriate adapters (#358614) ...
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bioRxiv - Microbiology 2024Quote: ... Samples were added to the top of a 60%-30%-10% sucrose/PBS gradient and centrifuged at 16,000 x g for 4.5 h at 4°C with an SW32 Ti rotor (Beckman, Brea, CA). Virus bands at the 60%-30% sucrose interface were collected via tube side puncture ...
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bioRxiv - Microbiology 2024Quote: ... tepidum culture was centrifuged at 10,000 x g for 10 min at 4°C in a JA-14 rotor (Beckman Coulter), and the supernatant was centrifuged again at 12,000 x g to remove cells and large debris ...
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bioRxiv - Molecular Biology 2024Quote: ... The gradient was centrifuged at 54,000 rpm for 3 h at 4 °C using a SW55 Ti rotor (Beckman Coulter). After centrifugation ...
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bioRxiv - Biochemistry 2024Quote: ... Lysates were clarified by ultracentrifugation for 35 minutes at 4℃ at 70,000 x (g) in a 45Ti rotor (Beckman Coulter). Constructs containing N-terminal TOG domains (TOG1 ...
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bioRxiv - Microbiology 2024Quote: ... sucrose gradients prepared with buffer C and centrifuged for 16 h at 71,935 g at 4 °C in a SW28 rotor (Beckman Coulter). Gradients were fractionated on a Biocomp Piston Gradient Fractionator (BioComp Instruments ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... containing cell nucleus and other heavy organelles was discarded and supernatant was centrifuged for 30 min at 48,000 × g at 4 °C (Beckman Avanti J-251 Ultra-centrifuge; Beckman Coulter). The supernatant was discarded ...
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bioRxiv - Neuroscience 2024Quote: ... The discontinuous sucrose gradient was centrifuged at 242,000 g for 47 min at 4 °C in a vertical rotor (VTi50 rotor, Beckman Coulter). The bulk GC fraction (GCF ...
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bioRxiv - Microbiology 2024Quote: ... Ribosomes were isolated by sucrose cushion ultracentrifuged at 100,000 rpm (543,000 × g) for 1 h at 4°C with a TLA110 rotor and an Optima MAX-TL ultracentrifuge (Beckman Coulter). Subsequently ...
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bioRxiv - Biophysics 2024Quote: ... the clarified lysate underwent a further centrifugation step at 205,000xg for 1 hour at 4 °C (60Ti rotor, Beckman Coulter). The membrane pellet was then resuspended in the resuspension buffer ...
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bioRxiv - Immunology 2024Quote: ... and centrifuged at 100,000 x g at 4°C for 90 min using the Optima™ XPN-80 Ultracentrifuge from Beckman Coulter ...
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bioRxiv - Biochemistry 2024Quote: ... Equivalent amounts of cell lysates prepared as described above were loaded onto the sucrose gradients and spun at 35,000 g for 3 hours at 4°C in a SW41 rotor (Beckman Coulter). After centrifugation the polysomes were fractionated and absorbance was monitored at 254 nm for RNA and 680 nm for GFP using the TriaxTM Flowcell (BioComp Instruments) ...
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bioRxiv - Cancer Biology 2024Quote: ... Samples were subjected to ultracentrifugation of 260,000 x g for 16 hours at 4°C using the Optima XPN-80 and SW40Ti rotor (Beckman Coulter). Twelve 1-mL fractions were collected from the top and subjected to immunoblotting.
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bioRxiv - Biophysics 2024Quote: ... the sample was separated by sucrose gradient ultracentrifugation with the gradients of 10 to 30 % sucrose and 0 to 0.2 % glutaraldehyde at 97,311 g at 4°C for 18hrs using an Optima™ XL-100K ultracentrifuge (Beckman Coulter). The fractions containing the complex were pooled and the sucrose was removed by using Amicon Ultra Centrifugal Filter ...