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Citations for Beckman :
751 - 800 of 877 citations for DNA Mismatch Repair Protein Msh3 MSH3 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: Library preparation was performed using Illumina NexteraXT™ DNA Sample Prep Kit per manufacturer’s protocol followed by AMPure XP (Beckman Coulter) purification ...
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bioRxiv - Developmental Biology 2021Quote: ... the libraries were further purified using the SPRIselect reagent to remove large DNA molecules (a right-side size selection with 0.55X followed by 1.5X ratios of beads) (Beckman Coulter, Brea, California). On average 10.6 millions of 38-nucleotide paired-end sequences were obtained using a NextSeq 500 sequencer (Illumina).
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bioRxiv - Immunology 2022Quote: ... Targeted amplification was performed for 13 cycles and the products were separated according to size into <400 bp (DNA barcode-tags) and >400 bp (the TCR sequences) using 0.6x SPRIselect beads (Beckman Coulter, B23318). Separate processing of the >400 bp bead-bound TCR sequences and the <400 bp in solution DNA barcodes was conducted according to manufacturer’s instruction and the TCR amplification products were sequenced on a NovaSeq running a 150 paired-end program ...
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bioRxiv - Genomics 2022Quote: ... 2 ug of each DNA sample was sheared to an approximate fragment size of 5000 bp and purified using AMPure XP beads (Beckman Coulter). Library preparation was performed using the NEBNext Ultra DNA Library Prep Kit (kit number E7370L ...
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bioRxiv - Cancer Biology 2022Quote: ... 50uL Qiagen buffer PB was added to each sample to stop the reaction and DNA was isolated with AMPure XP beads (Beckman Coulter). 15 cycles of PCR were performed with transposed DNA using the dual index primers and NEBNext PCR Master Mix ...
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bioRxiv - Genomics 2022Quote: ... The non-WGA sample was used to prepare an ONT library by shearing the DNA into ~8 kb fragments followed by purification and size-selection using AMPure XP beads (Beckman Coulter). The ONT library was then prepared with the Ligation Sequencing Kit (SQK-LSK109 ...
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bioRxiv - Genomics 2022Quote: ... The eluted chromatin was reverse-crosslinked overnight at 65°C and the DNA was purified with AMPure XP beads (Beckman Coulter).
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bioRxiv - Microbiology 2022Quote: ... a Y-linker [91] was annealed and ligated to the DNA and the samples were purified using Agencourt AMPure beads (Beckman Coulter). The Y-linker was annealed by combining the primers with annealing buffer in a PCR tube at 90°C for 2 min ...
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bioRxiv - Molecular Biology 2024Quote: ... The DNA was purified by phenol/chloroform and then fragments above 200 bp were selected by AMPure XP (BECKMAN COULTR, A63881). The end repair and adaptor ligation was performed by KAPA HyperPrep Kit (Roche ...
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bioRxiv - Cancer Biology 2024Quote: ... CRC cells were then cocultured at a 1:1 ratio with BMDCs for 4 hours and analyzed for uptake of CRC DNA by flow cytometry on the CytoFlex S cytometer (Beckman Coulter). Analysis was performed in FlowJo.
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bioRxiv - Microbiology 2022Quote: Samples were subjected to 750 μl of lysis buffer supplied with the GenFind V2 DNA extraction kit (Beckman Coulter, Indianapolis, U.S.). The suspension was vortexed for 10 min ...
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bioRxiv - Microbiology 2023Quote: Samples were subjected to 750 μl of lysis buffer supplied with the GenFind V2 DNA extraction kit (Beckman Coulter, Indianapolis, U.S.). The suspension was vortexed for 10 min ...
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bioRxiv - Microbiology 2023Quote: ... the DNA monitored on 2% agarose gel was purified by means of AMPure XT beads (Beckman Coulter Genomics, Danvers, MA, USA) and quantified by Qubit (Invitrogen ...
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bioRxiv - Biophysics 2023Quote: ... Samples were indexed using the IDT for Illumina Nextera DNA Unique Dual Indexes Set D (96 Indexes) and SPRISelect beads (Beckman Coulter) at a 0.9× ratio were used for cleanup and final size selection ...
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bioRxiv - Genomics 2022Quote: ... The mean red PI fluorescence of the stained DNA in the nuclei of the sample and the standards was quantified using a CytoFlex flow cytometer (Beckman Coulter). Haploid (1C ...
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bioRxiv - Cancer Biology 2023Quote: ... Crosslinks were reversed with 25 µg Proteinase K for 16 hours at 65°C and DNA was purified using AmpureXP beads (Beckman Coulter). Enrichment was confirmed using qRT-PCR with known positive and negative binding sites for each protein target ...
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bioRxiv - Microbiology 2023Quote: ... the DNA was diluted to 40 μl with TE buffer and purified with 1.5× vol of AMPure beads (Beckman Coulter, A63882). The eluted DNA was used for sequencing library preparation with NEBNext Ultra II DNA Library Prep Kit (New England BioLabs ...
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bioRxiv - Molecular Biology 2023Quote: ... the measure of DNA content and the percentage of GFP positive cells were carried out with Gallios flow cytometer (Beckman Coulter) using the Kaluza Acquisition software (Beckman Coulter) ...
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bioRxiv - Cell Biology 2023Quote: The genotypes of the chimeric mice were confirmed using genomic DNA extracted from GFP-negative fibroblasts obtained using FACS (MoFlo XDP, Beckman Coulter). MEFs were isolated from the entire fetus and head tissues on embryonic days (E ...
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bioRxiv - Molecular Biology 2024Quote: ... The ligated DNA (250 ± 25 bp) was size-selected using a bead-based method with magnetic beads (AmPure XP beads, Beckman Coulter) isolate DNA fragment sizes of interest ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Samples were homogenized using metal beads and a bead beater and DNA was extracted using the Agencourt DNAdvance kit (Beckman-Coulter). RNA was removed using RNase followed by an additional bead cleanup ...
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bioRxiv - Microbiology 2024Quote: Bacterial isolates were grown overnight on LB agar and DNA extraction was performed using the GenFind v3 gDNA extraction kit according to standard protocol (Supp. Table 4) (Beckman Coulter). For the mock microbial community ...
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bioRxiv - Microbiology 2024Quote: ... following a modification of the manufacturer’s protocol: DNA was purified between enzymatic reactions and library size selection was performed with AMPure XP beads (Beckman Coulter Genomics). The library concentrations were measured using the Qubit® dsDNA HS Assay Kit from Molecular Probes (Invitrogen ...
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bioRxiv - Genomics 2024Quote: ... to an average fragment size of 400 bp DNA was then subjected to a double-size selection to retain DNA fragments between 200 and 600 bp using Ampure XP beads (Beckman Coulter). Biotin-ligated DNA was precipitated with streptavidin-coupled magnetic beads (included in the kit) ...
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bioRxiv - Plant Biology 2024Quote: ... The index PCR was stopped after 8–10 cycles and the library DNA was purified using AMPure XP (A63880, Beckman Coulter). The library was sequenced on an Illumina NovaSeq 6000 instrument (150-bp paired-ends ...
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bioRxiv - Microbiology 2024Quote: One hundred nanograms of DNA was sheared to 550-700 bp using the Covaris LE220 and size selected using SPRI beads (Beckman Coulter). Ten nanograms of DNA for low input samples BottomLayer_2 and OS65 were sheared to 300 bp using the same selection ...
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bioRxiv - Evolutionary Biology 2024Quote: DNA was extracted from fin clips and embryos using the Agencourt DNAdvance magnetic bead-based purification system (Beckman Coulter, Brea, CA) in a 96-well plate format ...
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bioRxiv - Systems Biology 2024Quote: ... the DNA bound to chromatin was recovered by proteinase K treatment and crosslink reversal and purified using SPRIselect beads (Beckman Coulter). The NEB NEBNext Ultra II DNA Library Prep Kit was used for Illumina sequencing ...
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bioRxiv - Cancer Biology 2024Quote: ... DNA was stained with 25 μg/mL PI in PBS and cells were analyzed on a Gallios flow cytometer (Beckman Coulter) at the Montpellier Ressources Imagerie facility ...
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bioRxiv - Microbiology 2024Quote: ... was added to the supernatant on ice with constant stirring until protein precipitate was observed followed by centrifugation at 10,000 rpm at 4°C for 10 minutes (Beckman coulter, JA-25.50 rotor). The pellet was resuspended in 4 ml of binding buffer (20 mM Tris-HCl ...
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bioRxiv - Cancer Biology 2021Quote: ... The cells were stained with an antibody cocktail composed of CD64 FITC (22; Beckman Coulter (BC), Brea ...
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bioRxiv - Cell Biology 2021Quote: ... platelets in PRP were labeled with a mouse anti-human monoclonal antibody CD61-PE (Beckman Coulter). Platelet activation was detected by direct immunofluorescence labeling of alpha granule release marker CD62P (P-selectin ...
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bioRxiv - Cell Biology 2021Quote: Platelets in PRP were labeled with a mouse anti-human monoclonal antibody CD61-PE (Beckman Coulter). Incubation was performed at room temperature for 10 minutes in the dark ...
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bioRxiv - Immunology 2022Quote: ... the mRNA was separated from antibody barcodes using 0.6x AMPure XP machnetic beads (Beckman Coulter, USA). Then ...
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bioRxiv - Immunology 2023Quote: ... The cell subsets were identified using the following antibodies: CD64 PE-Cy7 (Cat. # B06025, Beckman Coulter); CD45 PerCP (Cat ...
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bioRxiv - Immunology 2024Quote: ... each including three distinct FITC and/or PE conjugated Vβ antibodies (Beckman Coulter, Brea, CA, USA) and a backbone panel of surface markers ...
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bioRxiv - Immunology 2024Quote: ... Cells were then stained with an HIV-1 Gag-p24 specific antibody (KC57-PE, Beckman Coulter). Sample acquisition and analysis were performed using a BD LSRFortessa and FlowJo v10.8 Software ...
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bioRxiv - Molecular Biology 2021Quote: ... Resulting libraries were pooled and treated with AMPure XP magnetic beads at a ratio of 0.8:1 beads:sample volume to purify DNA (Beckman Coulter, Brea, CA, USA). Library yield and quality was determined by TapeStation electrophoresis with HSD1000 ScreenTapes (Agilent Technologies).
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bioRxiv - Neuroscience 2021Quote: ... Before FACsorting we added the 5 ul of the cellular death dye Draq7TM (Viability dye, Far-red DNA intercalating agent, Beckman Coulter, #B25595). The suspension was sorted on an Astrios II cell sorter (Beckam Coulter) ...
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bioRxiv - Molecular Biology 2020Quote: ... The resulting cDNA was PCR-amplified to about 10–20 ng/μl using a high-fidelity DNA polymerase and purified with the Agencourt AMPure XP Kit (Beckman Coulter Genomics). The cDNA samples were pooled based on the ratios according to the RNA concentrations of the input samples ...
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bioRxiv - Cancer Biology 2020Quote: ... the DNA was eluted in 53 µL elution buffer and a second cleanup was performed using 50 µL of eluted DNA and 65 µL Agencourt AMPure XP magnetic beads (Beckman Coulter, A63880). The DNA was eluted with 25 µL elution buffer and 20 µL were used for the library amplification ...
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bioRxiv - Neuroscience 2020Quote: ... “Nucleosome free” and “mono-nucleosome fragments” were obtained by size-selection of DNA fragments between 170 and 400 bp using SPRIselect beads (Beckman Coulter #B23319) before single-end sequencing to generate 50 bp reads on the Hi-Seq 4000 platform (Illumina ...
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bioRxiv - Molecular Biology 2024Quote: ... Proteinase K digestion was performed and RNA-DNA chimeras precipitated from IP and input fractions before purification with AMPure XP beads (Beckman Coulter, A63882). RNA-DNA chimeras were digested with MmeI (NEB ...
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bioRxiv - Genetics 2023Quote: ... DNA was extracted from oral mucosal tissue using a commercial kit (chemagic™ DNA Buccal Swab Kit, PerkinElmer and DNAdvance Kit, Beckman Coulter). We performed real-time PCR to determine genotypes of DM-associated mutations (SOD1:c.118G
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bioRxiv - Genomics 2024Quote: ... The amplified full-length DNA library was purified to get rid of <500 bp fragments and other contaminants using AMPure XP beads (Beckman Coulter, A63881) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... The resulting cDNAs were PCR-amplified to about 10-20ng/µl using a high-fidelity DNA polymerase and purified using the Agencourt AMPure XP kit (Beckman Coulter Genomics). Pooled libraries were sequenced on an Illumina NextSeq 500 platform at the Core Unit SysMed at the University of Würzburg for bacterial in vitro samples and on an Illumina Novaseq at the genome analytics core facility of the Helmholtz Centre for Infection Research (HZI ...
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bioRxiv - Systems Biology 2023Quote: ... The DNA was purified and size selected to remove excess adaptors and adaptor dimers using Ampure XP beads (Beckman Coulter Life Sciences). Adaptor-ligated DNA was then subjected to PCR amplification with universal primers and an index barcode that was unique for each sample ...
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A major 6 Mb superlocus is involved in pyrethroid resistance in the common bed bug Cimex lectulariusbioRxiv - Evolutionary Biology 2023Quote: ... DNA purification was performed for each pool with 1.8 times the sample volume in AMPure XP beads (Beckman Coulter, Fullerton CA, USA). Purified DNA were retrieved in 100 µL of ultrapure water ...
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bioRxiv - Systems Biology 2023Quote: ... A-tailing and Y-adaptor ligation were performed following the protocol of (Bartlett et al., 2017): For the sample clean-up the DNA was purified using AMPure XP beads (Beckman Coulter, USA) instead of ethanol precipitation ...
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bioRxiv - Genomics 2023Quote: ... The tagmented DNA was amplified with eight cycles of PCR amplification and the libraries were cleaned up with SPRselect beads (Beckman Coulter, #B23317). The mean size of the library molecules and the quality of the libraries were determined on an Agilent Bio-analyser High Sensitivity DNA chip (Agilent technologies ...