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Citations for Beckman :
751 - 800 of 1754 citations for 7 Bromo 2 4 iodo phenyl imidazo 1 2 a pyridine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Cells were harvested by two rounds of centrifugation (6,000 rpm, 10 min, 4°C, JLA-9.1000 rotor; Beckman), resuspended in Buffer A containing 50 mM Tris-HCl pH8.0 ...
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bioRxiv - Cell Biology 2023Quote: ... pH 11.5) and were centrifuged for 60 min at 4 °C (150,000 g, Rotor TLA-100, Beckman Coulter). The yielded pellet was analyzed by immunoblotting and mass spectrometry (Supplementary Figure 1).
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bioRxiv - Cell Biology 2023Quote: ... pH 11.5) and is centrifuged for 60 min at 4 °C (150,000 g, Rotor TLA-100, Beckman Coulter). The resulting supernatant contains the released matrix proteins and peripheral membrane proteins ...
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bioRxiv - Cell Biology 2023Quote: ... pH 11.5) and was centrifuged for 60 min at 4 °C (150,000 g, Rotor TLA-100, Beckman Coulter). The resulting supernatant contains matrix proteins and peripheral membrane proteins ...
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bioRxiv - Cancer Biology 2023Quote: ... the high-speed centrifugation at 167,000g for 4 hours in an SW32 Ti swinging-bucket rotor (Beckman Coulter) was used to separate and further wash the EV from the concentrate collected in the previous step ...
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Faa1 membrane binding drives positive feedback in autophagosome biogenesis via fatty acid activationbioRxiv - Biochemistry 2023Quote: ... Lysates were cleared by centrifugation (20,000 rpm for 45 min at 4°C in a Beckman SW45Ti rotor). The supernatant was incubated with IgG Sepharose beads (GE Healthcare ...
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bioRxiv - Cell Biology 2023Quote: ... samples were centrifuged at 80,000 rpm for 20 min at 4°C in a TLA-100.1 rotor (Beckman). The supernatant was carefully removed ...
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bioRxiv - Cell Biology 2024Quote: ... The lysate was centrifuged at 20,000 rpm for 20 min at 4 °C in a JA 25.50 rotor (Beckman) and the supernatant was filtered through a 0.45 µm syringe filter ...
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bioRxiv - Microbiology 2024Quote: ... The eluent was ultracentrifuged (100,000 ×g, 4°C, 16 h) using Optima L-100 XP Ultracentrifuge (Beckman Coulter) to sediment ribosomes.
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bioRxiv - Molecular Biology 2024Quote: ... and centrifuged for 1.5 h at 75,000 rpm at 4°C using a TLA-120.2 rotor (Beckman Coulter). After carefully removing the supernatant ...
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bioRxiv - Neuroscience 2024Quote: ... the samples were ultracentrifuged (4 °C for 60 min) at 100,000×g (Optima MAX Preparative Ultracentrifuge, Beckman Coulter). Supernatants ...
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bioRxiv - Microbiology 2024Quote: ... it was concentrated by ultracentrifugation through OptiPrepTM Density Gradient Medium (D1556) at 154,000 g at 4℃ for 2.5 hours using a SW41Ti rotor (Beckman). The pelleted viral particles were suspended in 100 μL of PBS ...
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bioRxiv - Biochemistry 2024Quote: ... the purified particles were pelleted down by ultra-centrifugation at 30,000 rpm for 3.5 hours at 4 °C (Beckman Coulter ...
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bioRxiv - Microbiology 2021Quote: ... PE) and HIV-1 Gag core antigen (FH190-1-1, Beckman Coulter; PE, FITC). All samples were acquired on either an BD Fortessa X20 or LSR II using BD FACSDiva software and analyzed using FlowJo v10 (Tree Star) ...
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bioRxiv - Biophysics 2021Quote: ... Cell debris was removed by centrifugation (12,000x□g for 15□min at 4°C) and membranes were then pelleted at 45,000 rpm in a Type 45 Ti rotor (Beckman) for 3□h at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... Lysates were cleared by ultracentrifugation (40 000 rpm for 30 min at 4°C in a Beckman Ti45 rotor). Supernatant was filtered (0.45 μm ...
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bioRxiv - Biochemistry 2020Quote: ... Lysates were cleared by ultracentrifugation (25 000 rpm for 30 min at 4°C in a Beckman Ti45 rotor). Supernatant was incubated with GSH beads (GE Healthcare ...
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bioRxiv - Biochemistry 2021Quote: ... after which the lysate was clarified by centrifugation at 30,000 x g for 60 min at 4 °C in a JA 25.50 rotor in an Avanti J-E centrifuge (Beckman). Nucleic acids in the clarified lysate were precipitated by slowly adding polyethyleneimine with constant stirring in an ice bath to a final concentration of 0.4% ...
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bioRxiv - Microbiology 2020Quote: ... of 1.5 mL and then centrifuged 45 min at 100,000g at 4°C in a TLA-100 ultracentrifuge (Beckman Coulter). The supernatant contains the soluble fraction and the pellet corresponds to the total membranes ...
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bioRxiv - Cell Biology 2019Quote: ... The resulting gradients were subjected to ultracentrifugation at 4°C for 14 hrs at 41,000 rpm in an SW41 Ti rotor (Beckman), and then fractionated ...
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bioRxiv - Cell Biology 2020Quote: ... 0.5 mM DTT, 100 μg/ml cycloheximide) and centrifuged (160,000 g, 120 min, 4°C) in an SW41Ti rotor (Beckman). After centrifugation the sediment in the tube went through a UV detector at the wavelength of 254nm and simultaneously the required fractions (>=5 ribosomes ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The filtered supernatant from one 400 ml batch was then pelleted (1.5 hour at 24 000 rpm at 4 °C with Beckman Coulter L-90K centrifuge and 45 Ti rotor ...
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bioRxiv - Biophysics 2022Quote: ... The sample was loaded on top of the glycerol gradient and centrifuged at 4°C for 18 h at a speed of 35,000 rpm in SW-41Ti rotor (Beckman). Fractions of 500 μL were collected ...
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bioRxiv - Microbiology 2020Quote: ... Phage were pelleted by centrifugation at 82705 xg (25,000 rpm) for 4 hours using an SW28Ti rotor (Beckman Coulter). Phage pellets were gently resuspended overnight in 1 mL of phage buffer at 4°C with shaking ...
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bioRxiv - Genomics 2021Quote: ... we filtered the virus-laden media through a 0.22μm PES filter and then concentrated viral particles by ultracentrifugation at 24,000 rpm for 1.5 hours (4°C) with an SW-32Ti swinging bucket rotor (Beckman Coulter). We resuspended the viral pellet in iPS-Brew at 1/200th the original supernatant volume and then stored 50-100 μl aliquots at −80°C.
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bioRxiv - Immunology 2021Quote: Cells were analyzed using 4-to 6-color direct immunofluorescence staining on a Cytoflex LX or S (Beckman Coulter) flow cytometer ...
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bioRxiv - Microbiology 2021Quote: ... 200mM NaCl was used to top up the 13mL polyethylene tube and the solutions were spun at 27,000×g for 18 hours at 4°C using SW45 Ti rotor (Beckman). 1mL of top fraction ...
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bioRxiv - Biochemistry 2020Quote: ... and were centrifuged for 4 min at 46 000 rpm at 4°C in a Optima MAX-XP Ultracentrifuge with a TLA 100.3 rotor (Beckman). The pellet was suspended in (20 µl ...
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bioRxiv - Cancer Biology 2021Quote: ... Libraries were further amplified 4–6 PCR cycles as described [43] and purified using AMPure XP beads (Beckman Coulter). ATAC-seq libraries were subsequently sequenced 2 x 75 bp on an Illumina NextSeq500.
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bioRxiv - Biophysics 2021Quote: ... sucrose density gradients and centrifuged at 24,000 × rpm (102,170 × g) for 18 h at 4°C (Sw40Ti rotor, Beckman Coulter). The fractions of the sucrose gradient were applied to SDS-PAGE ...
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bioRxiv - Biophysics 2021Quote: ... The VP2 capsid protein fractions were pooled and subjected to centrifugation at 28,000 rpm (139,065 × g) for 3 h at 4°C (Sw40Ti rotor, Beckman Coulter). The pellet was resuspended in 50 mM Tris (pH 7.4) ...
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bioRxiv - Biochemistry 2020Quote: ... mixed for 10 minutes at 4°C and centrifuged at 25,000 rpm for 45 minutes (Beckman Ti-45 rotor). The supernatant was warmed to 37°C for few minutes until it turned cloudy following which it was centrifuged at 11,000 rpm at room temperature for 10 minutes (Beckman JA-20 rotor ...
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bioRxiv - Cell Biology 2021Quote: ... and centrifuged at 97,658 x g for 3 h at 4°C using a SW41Ti rotor (Beckman & Coulter, # 331362). The polysome fractions were collected using the Foxy R1 Fraction Collector and immediately snap frozen with liquid nitrogen and stored at −80 °C ...
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bioRxiv - Cell Biology 2021Quote: ... and centrifuged at 40,000 rpm (∼128,000 × g) for 3 h at 4°C using an MLS-5 rotor (Beckman Coulter). Aldolase (158 kDa ...
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bioRxiv - Biochemistry 2020Quote: ... Cell debris was removed by centrifugation at 14,800 rpm for 20 minutes 4 °C in F241.5P rotor using 149 Microfuge 22R centrifuge (Beckman Coulter). The supernatant was combined with 100 μL of ANTI-FLAG M2 Affinity Gel (Sigma ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were collected by centrifugation (8 000 rpm for 10 min at 4 °C, JLA-16.25 Beckman Coulter rotor), pellets frozen in liquid nitrogen and stored at −80°C ...
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bioRxiv - Microbiology 2020Quote: ... supernatants were harvested and filtered through a 0.45μm filter and centrifuged at 27,000 rpm for 3h at 4°C with a SW41 rotor and Optima L-90 centrifuge (Beckman). Pellets were resuspended in PBS prior to use for western blot analysis.
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bioRxiv - Microbiology 2020Quote: ... The gradient was centrifuged for 17 h at 4°C and 100,000 rcf (23,700 rpm) using an SW 40 Ti rotor (Beckman Coulter), followed by manual fractionation into 20 590 μl fractions and measurement of the A260 nm of each fraction ...
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bioRxiv - Plant Biology 2020Quote: ... 50 μg/ml cycloheximide) and centrifuged for 3 h at 55,000 g and 4 °C in a Type 70 Ti rotor (Beckman). The pellet was dissolved in 1% SDS ...
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bioRxiv - Microbiology 2020Quote: ... the 100,000xg centrifugation was performed for 1h at 4°C using a SW41 TI swinging bucket rotor (Beckman Coulter). The pellet was then resuspended in 50 μL or 1500 μL of D-MEM for subsequent analysis.
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bioRxiv - Neuroscience 2019Quote: ... of the lysates were loaded on top of the gradients and spun at 36,000 rpm at 4°C for 2h with a SW41-Ti rotor (Beckman). Fractions from each sample were collected every 9 sec using a density gradient fractionation system (Teledyne Isco ...
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bioRxiv - Microbiology 2021Quote: ... Filtered supernatants were pelleted at 60,000 g for 16 hours at 4 °C in a SW28 Ti rotor (Beckman), and the resulting pellet was resuspended in TNE buffer (50 mM Tris-HCl [pH 7.4] ...
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bioRxiv - Molecular Biology 2020Quote: ... The reactions were then spun for 20 min at 120,000 rpm at 4°C (TLA120.1 rotor, Beckman Coulter, USA) to remove aggregates and ribosomes ...
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bioRxiv - Microbiology 2020Quote: ... Supernatant was transferred to a fresh tube and further clarified by centrifugation at 18,000 x g for 10 min at 4°C in a microfuge (Beckman), and supernatant transferred to a fresh tube.
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bioRxiv - Microbiology 2020Quote: ... Cell lysate was then clarified 200 x g for 10 min at 4°C in an Allegra centrifuge (Beckman). Supernatant was transferred to a fresh tube and further clarified by centrifugation at 18,000 x g for 10 min at 4°C in a microfuge (Beckman) ...
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bioRxiv - Cell Biology 2022Quote: ... The lysate was clarified by centrifugation (183,960 x g, 88 min, 4°C) in a Type 70Ti rotor (Beckman). The supernatant was mixed with 2 ml of IgG Sepharose 6 Fast Flow beads (GE Healthcare Life Sciences ...
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bioRxiv - Molecular Biology 2022Quote: ... Gradients were spun for 3 hours at 4° C and 36,000 RPM (221,632 x g) on SW41-Ti rotor (Beckman Coulter). Monosome fractions were isolated from each sucrose gradient with Brandel Density Gradient Fractionator using 1.5 mL/min flow speed and 60% CsCl ...
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bioRxiv - Cell Biology 2022Quote: ... The lysate was clarified via centrifugation (66,000 x g, 30 min, 4°C) in a Type 70 Ti rotor (Beckman). The supernatant was mixed with 1.5 ml of anti-FLAG M2 affinity gel (Sigma-Aldrich ...
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bioRxiv - Microbiology 2022Quote: ... sonicated using a sonicator (Smurt, Microtec) and ultracentrifuged at 100,000 x g for 60 minutes at 4°C (Optima XE-90, Beckman). Protein concentration of the bacterial membrane suspension was determined with Bio-Rad protein assay (BioRad).
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bioRxiv - Cell Biology 2022Quote: ... and centrifuged at 10,000g and 4°C for 30 minutes (SW41 rotor, Optima XPN-100 ultracentrifuge; Beckman Coulter, Inc.) to remove any potential large debris ...