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Citations for Beckman :
751 - 800 of 1483 citations for 5 Bromo 2 Tert Butyldimethylsilyl 4 Methylthiazole since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... for 15 minutes and centrifuged at 10,000 x g for 25 minutes at 4°C (JA20 rotor, Beckman Coulter). The supernatant containing the recombinant proteins was filtered through a 0.45 µm sterile filter (Whatman Puradisc) ...
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bioRxiv - Biophysics 2024Quote: ... The supernatant was then centrifuged for 1 h at 38000 rpm and 4 °C in a TI45 rotor (Beckman) to pellet the membranes ...
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bioRxiv - Biophysics 2024Quote: Tau proteins were diluted in cold 1x BRB80 to a concentration of a few tenths of micromolar and centrifuged to remove aggregates (10 min/4°C /215,000 x g in Type 70 Ti rotor [Beckman]). Prior to use ...
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bioRxiv - Biophysics 2024Quote: ... The lysate was clarified via centrifugation (66,000 x g, 30 min, 4°C) in a Type 70 Ti rotor (Beckman), before being incubated overnight at 4°C with 1.5 mL of anti-FLAG M2 affinity gel (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2024Quote: ... The crude membrane fraction containing MsbA was isolated by ultracentrifugation (100000g, 45 min, 4°C, Beckman Coulter rotor Ti70). About 2 mg of MsbA-enriched membranes were solubilized with 1% DDM (w/v ...
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bioRxiv - Cancer Biology 2024Quote: ... Samples were centrifuged at 24,000 rpm under vacuum for 1.5 hrs at 4°C (Allegra X-15R Centrifuge, Beckman Coulter). The viral pellet was resuspended in 300 µL PBS ...
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bioRxiv - Biophysics 2024Quote: ... Lysis was accomplished via douncing and the lysate was clarified via centrifugation (183,960 x g, 88 min, 4°C) in a Type 70Ti rotor (Beckman). 2 mL of IgG Sepharose 6 Fast Flow beads (Cytiva ...
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bioRxiv - Cell Biology 2024Quote: ... and the lysate was cleared twice by centrifugation at 40,000 rcf for 20 min each at 4°C using a JA 25.50 rotor (Beckman Coulter). The cleared lysate was incubated with 2 mL Ni-NTA resin (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... 36 mL of media per condition was subject to ultracentrifugation at 100,000 x g for 6 hours at 4 °C in a SW-32-Ti swinging bucket rotor (Beckman). The media was decanted and 500 μL PBS was added to the pellet and left to resuspend overnight at 4 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... samples were loaded based on equal total RNA onto a 10%–50% sucrose gradient and subjected to ultracentrifugation (187,813 g for 1.5 hours at 4 °C) using a SW 41 Ti rotor (Beckman Coulter). Subsequently ...
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bioRxiv - Bioengineering 2024Quote: ... The virus was then pelleted by centrifugation at 4°C for 45 min at 25,000 rpm in a sw28 rotor (Beckman) and gently suspended in dilution buffer (150 mM NaCl ...
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bioRxiv - Cancer Biology 2024Quote: ... which was then centrifuged at 100,000 × g for 18 hours at 4°C (344060, SW 40 Ti, Beckman Coulter) for further purification ...
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bioRxiv - Cell Biology 2024Quote: ... All ultracentrifugation steps will be performed at 4°C using a SW41 Ti rotor (Beckman Coulter, Brea, CA, USA).
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bioRxiv - Cell Biology 2024Quote: ... The 100,000xg SEV-containing pellets were resuspended in 3 ml sterile cold PBS and repelleted at 100,000xg for 4 hrs in a TLA110 rotor (Beckman). SEVs were analyzed for size and number by nanoparticle tracking (ZetaView ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Ribosomes were pelleted by centrifugation at 27,000 rpm (57,000 xg) for 16 h at 4 °C in a Ti-45 rotor (Beckman-Coulter). Ribosomes pellets were then resuspended in dissociation buffer (20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were harvested at day 3 post-infection by centrifuging at 2,000xg for 15 mins at 4°C using a JLA 8.1000 rotor (Beckman Coulter). Insect cell pellets were frozen in liquid nitrogen for storage or used immediately for protein purification.
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bioRxiv - Cell Biology 2024Quote: ... Cells were harvested by centrifuging at 5,000xg for 15 mins at 4°C using a JLA 8.1000 rotor (Beckman Coulter). Bacterial cell pellets were frozen in liquid nitrogen for storage or used immediately for protein purification.
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bioRxiv - Cell Biology 2024Quote: ... Lysates were clarified by centrifugation at 20,000 x g for 45 mins at 4°C in a JA25.50 rotor (Beckman Coulter). Clarified lysates were applied to a gravity flow column several times over 2 ml Ni-NTA resin (Sigma ...
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bioRxiv - Synthetic Biology 2021Quote: ... 72C for 2’) and a X0.65 SPRI bead DNA clean up (AMPure XP, Beckman Coulter) a second PCR using a p5 primer (F6 ...
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bioRxiv - Systems Biology 2022Quote: ... Final libraries were size-selected by performing 2-step bead cleaning (SPRIselect, Beckman Coulter B23318) to remove larger DNA fragments and primer dimers and quality was assessed by Bioanalyzer (Agilent 2100 ...
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bioRxiv - Immunology 2022Quote: ... and SARS-CoV-2 RBD+ were sorted with MoFlo Astrios EQ Cell Sorter (Beckman Coulter).
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bioRxiv - Immunology 2022Quote: ... and SARS-CoV-2 RBD+ were sorted with MoFlo Astrios EQ Cell Sorter (Beckman Coulter). FACS data were analyzed using FlowJo™ v10.8 (BD Biosciences).
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bioRxiv - Genomics 2022Quote: ... then ultracentrifuge at 24,000 rpm for 2 h using SW28 swinging bucket rotor (Beckman Coulter). Upon completion ...
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bioRxiv - Microbiology 2022Quote: ... The filtrate was then ultracentrifuged 100,000 g for 2 hours (Optima L-90K, Beckman Coulter). The vesicle pellet was resuspended in 100 μl of sterile PBS.
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bioRxiv - Genomics 2023Quote: ... then ultracentrifuge at 24,000 rpm for 2 h using SW28 swinging bucket rotor (Beckman Coulter). Upon completion ...
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bioRxiv - Genomics 2024Quote: ... Polyadenylated RNA was purified using 2× sample volume of AMPureXP RNA beads (Beckman Coulter, A66514), following the manufacturer’s protocol ...
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An anti-amyloidogenic treatment to specifically block the consolidation of traumatic events in mousebioRxiv - Animal Behavior and Cognition 2020Quote: ... the rest of the volume was centrifuged for 5 minutes at 4,000 rpm (Microfugue 18 centrifuge, Beckman Coulter) to obtain the cell debris fraction (P1 ...
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bioRxiv - Biochemistry 2021Quote: ... The labeled peptides were further purified by C-18 (5 μm, 4.6 mm × 150 mm, Beckman Coulter Inc.) reverse phase-high performance liquid chromatography ...
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bioRxiv - Biochemistry 2020Quote: ... After 20 minutes the cells were collected by centrifugation (8,000 rpm, 5 minutes, JLA-16.25 Beckman Coulter rotor), dissolved in 0.5 mL of HEPES:Polymix buffer (5 mM Mg(OAc)2 ...
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bioRxiv - Genetics 2021Quote: ... Reactions were then pooled in a 5 ml tube and purified using a 0.7x SPRIselect (Beckman Coulter B23318) cleanup and eluted in 450μL of TE ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and washed in 5 volumes of chloroplast wash buffer (8 °C, manual stop when reaching 3000 RPM, Beckman JA-20 ...
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bioRxiv - Molecular Biology 2023Quote: ... Dialyzed nucleosomes were purified in a 5-30% w/v sucrose gradient with a SW41 Ti (Beckman Coulter) rotor in an Optima L-90K ultracentrifuge (Beckman Coulter ...
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bioRxiv - Cell Biology 2024Quote: ... 600 µL of the lysate were transferred to an ultracentrifuge tube (5 x 41 mm, Beckman-Coulter, #344090) and centrifuged in an MLS-50 rotor (Beckman-Coulter ...
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bioRxiv - Genetics 2024Quote: ... followed by inactivation at 85° for 5 min and cDNA purification with AMPure XP beads (Beckman Coulter, A63880) at a 1:1.1 sample-to-bead ratio ...
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bioRxiv - Biochemistry 2021Quote: ... 10 U/mL SuperASE-In and was ultracentrifuged at 100,000 × g for for 16 h at 4 °C in a SW Ti41 rotor (Beckman Coulter). After removing the supernatant ...
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bioRxiv - Biochemistry 2021Quote: ... The lysate was clarified by ultracentrifugation at 30,000 × g 30 min at 4°C using a Ti45 rotor (Beckman Coulter) twice ...
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bioRxiv - Biophysics 2020Quote: ... Escherichia coli BL21 cells overexpressing α-synuclein were collected by centrifugation (20 min, 4000 rpm, 4 °C) in a JLA-8.1000 rotor in a Beckman Avanti J25 centrifuge (Beckman Coulter), resuspended in lysis buffer (10 mM Tris ...
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bioRxiv - Biophysics 2020Quote: ... The insect cells were harvested after 4 days by centrifugation at 300 g for 10 min at 4 °C in an Avanti J-26S ultracentrifuge (JLA-9.1000 rotor, Beckman Coulter). The cell pellet was resuspended in 5 ml ice-cold phosphate buffered saline (PBS ...
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bioRxiv - Biophysics 2020Quote: ... and centrifuged at 45000 x g for 60 min at 4 °C in the Avanti J-26S ultracentrifuge (JA-30.50Ti rotor, Beckman Coulter). The cleared cell lysate was incubated in a lysis buffer-equilibrated Ni-NTA column (HisPur Ni-NTA Superflow Agarose ...
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bioRxiv - Cell Biology 2020Quote: ... Crude mitochondria were loaded on top of the chilled gradient and separated at 100,000 Å∼ g for 1 h at 4°C (SW41 rotor; Beckman). Intact purified mitochondria were recovered from a brown band at around 16% Histodenz™ concentration ...
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bioRxiv - Cell Biology 2020Quote: ... and the gradient was centrifuged for 60 min at 24,700 × g at 4 °C using a TLS-55 swinging bucket rotor (Beckman Coulter). Purified mouse POS were resuspended in DMEM/Ham’s F-12 containing 2.5% sucrose ...
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bioRxiv - Cell Biology 2020Quote: ... lysed by sonication (UD-201 ultrasonic disrupter, Tomy Seiko), and centrifuged at 50,000 rpm (70 min, 4°C) with a 70 Ti rotor (Beckman Coulter). GST-His6-tagged LC3B-His12 and GATE-16-His12 proteins in the supernatants obtained were isolated by mixing with COSMOGEL GST-Accept beads (50% slurry ...
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bioRxiv - Immunology 2021Quote: ... the culture medium containing the lentiviral particles was collected and ultracentrifuged at 25,000 rpm for 90 min at 4 °C using a Beckman SW28 ultracentrifuge rotor (Beckman Coulter) for the obtention of concentrated virus ...
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bioRxiv - Microbiology 2020Quote: ... sanguinis was grown as described above and centrifuged for 10 minutes at 3,740 x g in an Allegra X-142 centrifuge at 4°C (Beckman-Coulter). The supernatant was discarded and the cells were resuspended in BHI prior to inoculation ...
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bioRxiv - Neuroscience 2021Quote: ... PMCA products were centrifuged at 45,000 rpm for 2.5 h at 4 °C (Optima™ Max Ultracentrifuge, Beckman Coulter, USA), the supernatants discarded and the pellets resuspended in an equal volume of PBS.
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bioRxiv - Molecular Biology 2021Quote: ... 2 mM DTT and centrifuged for 16 h at 34,000 rpm at 4°C for 16 h using an SW60 Ti rotor (Beckman Coulter). The fractions were subjected to SDS-PAGE and Coomassie blue staining.
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bioRxiv - Molecular Biology 2021Quote: ... The samples were centrifuged at 34,000 rpm at 4°C for 16 h using an SW60 Ti rotor (Beckman Coulter). Subsequently ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 mM DTT and centrifuged for 16 h at 34,000 rpm at 4°C for 16 h using an SW60 Ti rotor (Beckman Coulter). The fractions ...
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bioRxiv - Molecular Biology 2020Quote: ... Gradients were centrifuged at 39 000 rpm for 2.5 h at 4°C in an Optima L-100XP ultracentrifuge (Beckman– Coulter) using the SW41Ti rotor with brake ...
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bioRxiv - Cell Biology 2020Quote: ... The insect cells were harvested after 4 days by centrifugation at 300 g for 10 min at 4 °C in an Avanti J-26S ultracentrifuge (JLA-9.1000 rotor, Beckman Coulter). The cell pellet was resuspended in 5 ml ice-cold phosphate buffered saline (PBS ...