Labshake search
Citations for Beckman :
751 - 800 of 1497 citations for 5 4 Iodophenoxy methyl furan 2 carboxylic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... were centrifuged at approximately 108,000 x g for 107 min at 4°C using an ultracentrifuge device equipped with a TLA 100.3 fixed-angle rotor (Beckman). The supernatants were retained as soluble fractions and kept at 4°C for downstream analyses ...
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bioRxiv - Microbiology 2024Quote: ... Phage particles were pelleted by centrifugation at 75,000 x g at 4°C for 1 h using a 50.2 Ti rotor (Beckman Coulter) and resuspended in the phage buffer.
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bioRxiv - Neuroscience 2024Quote: ... The homogenate was layered on the gradient and centrifuged (3h, 4°C, 160,000 xg) using a SW40 rotor (Beckman). Top layer of debris was removed and ∼18 fractions of 500 µl were collected from each gradient from top (fraction 1 ...
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bioRxiv - Cell Biology 2024Quote: ... 0.1 mM PMSF buffer and subjected to ultracentrifugation at 36,000 rpm at 4°C for 2h using a SW41 rotor (Beckman) and 14 x 89 mm polypropylene tubes (Beckman ...
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bioRxiv - Biophysics 2024Quote: ... Lysates were clarified by ultracentrifugation at 34,000 rpm for 45 minutes at 4°C in a Type 45.1 Ti rotor (Beckman-Coulter). The supernatants were then passed through a 0.22 μm syringe sterile filter (Thermo-Fisher ...
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Syntaxin-2 inhibits excessive phagocytic uptake but promotes phagolysosomal clearance in macrophagesbioRxiv - Cell Biology 2024Quote: ... The samples were then centrifuged at 100,000 g for 1 hr at 4°C using a SW40Ti rotor (Beckman). Phagosomes were recovered from the interface of 10 and 25% sucrose (Fig ...
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bioRxiv - Cell Biology 2024Quote: ... and the membranes were pelleted at 100,000 g for one hour at 4°C in a TLA 100.3 rotor (Beckman-Coulter). Afterwards the supernatant was taken off and stored at −20°C (“S100” ...
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bioRxiv - Biochemistry 2024Quote: ... The unbroken cells and debris were removed by centrifugation at 20,000 x g for 10 min at 4 °C (Beckman Coulter Avanti J20 XPI centrifuge ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... containing cell nucleus and other heavy organelles was discarded and supernatant was centrifuged for 30 min at 48,000 × g at 4 °C (Beckman Avanti J-251 Ultra-centrifuge ...
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bioRxiv - Neuroscience 2024Quote: ... Tubes were centrifugated at 100,000 x g (32,500 RPM) for 3 hours at 4°C using a SW55Ti rotor (Beckman Coulter). Synaptosomes enriched in the interface between 1.0-1.25 M sucrose were collected and diluted with ice-cold 0.1 mM CaCl2 ...
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bioRxiv - Neuroscience 2024Quote: ... The supernatant was passed through a 0.22 μm filter before undergoing ultracentrifugation at 100,000g for 70 min at 4°C (Optima-XE SW41 Beckman Coulter). The resulting pellet was then resuspended in 2 ml of 0.475 M sucrose solution prepared in double-filtered PBS ...
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bioRxiv - Microbiology 2024Quote: ... the supernatants were loaded onto 1 mL of 30% sucrose in PBS buffer and centrifuged at 25,000 rpm at 4°C for 3 hours using the 90AT rotor (Beckman). The pelleted viral particles were resuspended in 100 μL of PBS and fixed with formaldehyde (working concentration 0.1% ...
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bioRxiv - Bioengineering 2024Quote: ... the suspension was centrifuged at 10,000 RPM for 10 min at first and 30,000 RPM for 4 hours (Optima XPN-80, Beckman Coulter), and the supernatant was collected to remove the precipitates ...
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bioRxiv - Immunology 2024Quote: ... Sartorus) at 100,000 x g for 70 min at 4°C using the ultracentrifuge Optima MAX-TL (Beckman Coulter). The pellets were suspended in PBS(– ...
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bioRxiv - Bioengineering 2024Quote: ... for 15 minutes and centrifuged at 10,000 x g for 25 minutes at 4°C (JA20 rotor, Beckman Coulter). The supernatant containing the recombinant proteins was filtered through a 0.45 µm sterile filter (Whatman Puradisc) ...
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bioRxiv - Biophysics 2024Quote: ... The supernatant was then centrifuged for 1 h at 38000 rpm and 4 °C in a TI45 rotor (Beckman) to pellet the membranes ...
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bioRxiv - Biophysics 2024Quote: Tau proteins were diluted in cold 1x BRB80 to a concentration of a few tenths of micromolar and centrifuged to remove aggregates (10 min/4°C /215,000 x g in Type 70 Ti rotor [Beckman]). Prior to use ...
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bioRxiv - Biophysics 2024Quote: ... The lysate was clarified via centrifugation (66,000 x g, 30 min, 4°C) in a Type 70 Ti rotor (Beckman), before being incubated overnight at 4°C with 1.5 mL of anti-FLAG M2 affinity gel (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2024Quote: ... The crude membrane fraction containing MsbA was isolated by ultracentrifugation (100000g, 45 min, 4°C, Beckman Coulter rotor Ti70). About 2 mg of MsbA-enriched membranes were solubilized with 1% DDM (w/v ...
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bioRxiv - Cancer Biology 2024Quote: ... Samples were centrifuged at 24,000 rpm under vacuum for 1.5 hrs at 4°C (Allegra X-15R Centrifuge, Beckman Coulter). The viral pellet was resuspended in 300 µL PBS ...
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bioRxiv - Biophysics 2024Quote: ... Lysis was accomplished via douncing and the lysate was clarified via centrifugation (183,960 x g, 88 min, 4°C) in a Type 70Ti rotor (Beckman). 2 mL of IgG Sepharose 6 Fast Flow beads (Cytiva ...
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bioRxiv - Cell Biology 2024Quote: ... and the lysate was cleared twice by centrifugation at 40,000 rcf for 20 min each at 4°C using a JA 25.50 rotor (Beckman Coulter). The cleared lysate was incubated with 2 mL Ni-NTA resin (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... 36 mL of media per condition was subject to ultracentrifugation at 100,000 x g for 6 hours at 4 °C in a SW-32-Ti swinging bucket rotor (Beckman). The media was decanted and 500 μL PBS was added to the pellet and left to resuspend overnight at 4 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... samples were loaded based on equal total RNA onto a 10%–50% sucrose gradient and subjected to ultracentrifugation (187,813 g for 1.5 hours at 4 °C) using a SW 41 Ti rotor (Beckman Coulter). Subsequently ...
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bioRxiv - Bioengineering 2024Quote: ... The virus was then pelleted by centrifugation at 4°C for 45 min at 25,000 rpm in a sw28 rotor (Beckman) and gently suspended in dilution buffer (150 mM NaCl ...
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bioRxiv - Cancer Biology 2024Quote: ... which was then centrifuged at 100,000 × g for 18 hours at 4°C (344060, SW 40 Ti, Beckman Coulter) for further purification ...
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bioRxiv - Cell Biology 2024Quote: ... All ultracentrifugation steps will be performed at 4°C using a SW41 Ti rotor (Beckman Coulter, Brea, CA, USA).
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bioRxiv - Cell Biology 2024Quote: ... The 100,000xg SEV-containing pellets were resuspended in 3 ml sterile cold PBS and repelleted at 100,000xg for 4 hrs in a TLA110 rotor (Beckman). SEVs were analyzed for size and number by nanoparticle tracking (ZetaView ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Ribosomes were pelleted by centrifugation at 27,000 rpm (57,000 xg) for 16 h at 4 °C in a Ti-45 rotor (Beckman-Coulter). Ribosomes pellets were then resuspended in dissociation buffer (20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were harvested at day 3 post-infection by centrifuging at 2,000xg for 15 mins at 4°C using a JLA 8.1000 rotor (Beckman Coulter). Insect cell pellets were frozen in liquid nitrogen for storage or used immediately for protein purification.
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bioRxiv - Cell Biology 2024Quote: ... Cells were harvested by centrifuging at 5,000xg for 15 mins at 4°C using a JLA 8.1000 rotor (Beckman Coulter). Bacterial cell pellets were frozen in liquid nitrogen for storage or used immediately for protein purification.
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bioRxiv - Cell Biology 2024Quote: ... Lysates were clarified by centrifugation at 20,000 x g for 45 mins at 4°C in a JA25.50 rotor (Beckman Coulter). Clarified lysates were applied to a gravity flow column several times over 2 ml Ni-NTA resin (Sigma ...
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bioRxiv - Synthetic Biology 2021Quote: ... 72C for 2’) and a X0.65 SPRI bead DNA clean up (AMPure XP, Beckman Coulter) a second PCR using a p5 primer (F6 ...
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bioRxiv - Systems Biology 2022Quote: ... Final libraries were size-selected by performing 2-step bead cleaning (SPRIselect, Beckman Coulter B23318) to remove larger DNA fragments and primer dimers and quality was assessed by Bioanalyzer (Agilent 2100 ...
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bioRxiv - Immunology 2022Quote: ... and SARS-CoV-2 RBD+ were sorted with MoFlo Astrios EQ Cell Sorter (Beckman Coulter).
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bioRxiv - Immunology 2022Quote: ... and SARS-CoV-2 RBD+ were sorted with MoFlo Astrios EQ Cell Sorter (Beckman Coulter). FACS data were analyzed using FlowJo™ v10.8 (BD Biosciences).
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bioRxiv - Genomics 2022Quote: ... then ultracentrifuge at 24,000 rpm for 2 h using SW28 swinging bucket rotor (Beckman Coulter). Upon completion ...
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bioRxiv - Microbiology 2022Quote: ... The filtrate was then ultracentrifuged 100,000 g for 2 hours (Optima L-90K, Beckman Coulter). The vesicle pellet was resuspended in 100 μl of sterile PBS.
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bioRxiv - Genomics 2023Quote: ... then ultracentrifuge at 24,000 rpm for 2 h using SW28 swinging bucket rotor (Beckman Coulter). Upon completion ...
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bioRxiv - Genomics 2024Quote: ... Polyadenylated RNA was purified using 2× sample volume of AMPureXP RNA beads (Beckman Coulter, A66514), following the manufacturer’s protocol ...
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An anti-amyloidogenic treatment to specifically block the consolidation of traumatic events in mousebioRxiv - Animal Behavior and Cognition 2020Quote: ... the rest of the volume was centrifuged for 5 minutes at 4,000 rpm (Microfugue 18 centrifuge, Beckman Coulter) to obtain the cell debris fraction (P1 ...
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bioRxiv - Biochemistry 2021Quote: ... The labeled peptides were further purified by C-18 (5 μm, 4.6 mm × 150 mm, Beckman Coulter Inc.) reverse phase-high performance liquid chromatography ...
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bioRxiv - Biochemistry 2020Quote: ... After 20 minutes the cells were collected by centrifugation (8,000 rpm, 5 minutes, JLA-16.25 Beckman Coulter rotor), dissolved in 0.5 mL of HEPES:Polymix buffer (5 mM Mg(OAc)2 ...
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bioRxiv - Genetics 2021Quote: ... Reactions were then pooled in a 5 ml tube and purified using a 0.7x SPRIselect (Beckman Coulter B23318) cleanup and eluted in 450μL of TE ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and washed in 5 volumes of chloroplast wash buffer (8 °C, manual stop when reaching 3000 RPM, Beckman JA-20 ...
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bioRxiv - Molecular Biology 2023Quote: ... Dialyzed nucleosomes were purified in a 5-30% w/v sucrose gradient with a SW41 Ti (Beckman Coulter) rotor in an Optima L-90K ultracentrifuge (Beckman Coulter ...
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bioRxiv - Cell Biology 2024Quote: ... 600 µL of the lysate were transferred to an ultracentrifuge tube (5 x 41 mm, Beckman-Coulter, #344090) and centrifuged in an MLS-50 rotor (Beckman-Coulter ...
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bioRxiv - Genetics 2024Quote: ... followed by inactivation at 85° for 5 min and cDNA purification with AMPure XP beads (Beckman Coulter, A63880) at a 1:1.1 sample-to-bead ratio ...
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bioRxiv - Biochemistry 2021Quote: ... 10 U/mL SuperASE-In and was ultracentrifuged at 100,000 × g for for 16 h at 4 °C in a SW Ti41 rotor (Beckman Coulter). After removing the supernatant ...
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bioRxiv - Biochemistry 2021Quote: ... The lysate was clarified by ultracentrifugation at 30,000 × g 30 min at 4°C using a Ti45 rotor (Beckman Coulter) twice ...