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Citations for Beckman :
701 - 750 of 811 citations for Integrin beta 1 binding protein 1 ITGB1BP1 Antibody Biotin since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... The 1 ml fraction containing the phage was then placed on a 40% (w/v) - 60% (w/v) sucrose gradient for 1 hour at 63,400 x g at 4°C (Beckman Coulter SW 40 Ti). The 1 ml fraction was then placed in a 50 kDa dialysis membrane (Spectrum Labs ...
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bioRxiv - Biophysics 2024Quote: ... The cell lysate was centrifuged at 4 °C at 35000 rpm for 1 hour with a Beckman OptimaTM L-80XP Ultracentrifuge equipped with a Ti45 rotor (Beckman, Brea, CA, USA). The pellet containing membranes and cell debris was discarded ...
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bioRxiv - Immunology 2024Quote: ... Cells were then washed in FACS buffer and fixed in 1% formalin before being suspended in FACS buffer and analyzed using a BD LSR II FACS system and Cytoflex (Beckman Coulter, Memorial University). Cell populations were gated based on viability dye and subjected to forward and side scattering to identify single cell population before additional analysis using Flowjo (BD Biosciences).
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bioRxiv - Biochemistry 2024Quote: ... Lysates were centrifuged at 26,000 xg for 25 min (Sorvall RC 5B Plus) followed by membrane isolation through ultracentrifugation at 55,000 pm for 1 h (Beckman, Type 70 Ti Rotor). Membranes were resuspended in HBS by mortar and pestle at 20 mL/g and snap-frozen in LN2 before storage at -80 °C until further use ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were centrifuged at 136,000 x g for 1 hour at ambient temperature using a Beckman Coulter Optima MAX-XP Ultracentrifuge with a TLA-55 rotor (Beckman Coulter, CA, USA). The resulting sarkosyl-insoluble pellet was washed in homogenization buffer containing 1% sarkosyl ...
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bioRxiv - Biochemistry 2024Quote: ... The A2aR-expressing membrane fraction was obtained by ultra- centrifugation at 100,000 g for 1 hour at 4°C in a Type 70 Ti Fixed-angle rotor (Optima XPN ultracentrifuge, Beckman Coulter Life Sciences). The pellet (membrane fraction ...
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bioRxiv - Genomics 2020Quote: The level of MPL protein on the platelet membrane was measured by flow cytometry (Beckman Coulter FC500) using the monoclonal antibodies ...
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bioRxiv - Immunology 2020Quote: CCMV-SS and CCMV-Ctt830-SS VLPs were separated from cellular proteins by ultracentrifugation (SW28 rotor, Beckman, Palo Alto ...
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bioRxiv - Microbiology 2023Quote: ... The surface expression level of S proteins (Figure 4B) was measured using CytoFLEX Flow Cytometer (Beckman Coulter) and the data were analyzed using FlowJo software v10.7.1 (BD Biosciences) ...
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bioRxiv - Cancer Biology 2022Quote: Antibodies used included: Hematopoietic panel: Lineage-PE (Beckman Coulter, Cat#B29559), CD34-APC (BioLegend Cat# 343607 ...
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bioRxiv - Immunology 2020Quote: ... Antibody labelled cells were analyzed by flow cytometry (Navios; Beckman Coulter). Flow cytometric data analysis was performed with the help of Kaluza Software 1.5a (Beckman Coulter).
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bioRxiv - Immunology 2020Quote: ... Cells were further stained with an anti-CD45RO antibody (Beckman Coulter).
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bioRxiv - Microbiology 2022Quote: ... fixed cells were incubated with FITC-p24 antibody (KC57; Beckman Coulter) in the presence of 0.1% Triton-X ...
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bioRxiv - Molecular Biology 2020Quote: ... bound antibodies were visualized using 100 µL of a 1:100 dilution of goat F(ab’)2 anti-human IgG coupled to phycoerythrin (Beckman Coulter, Brea, CA, USA) at 4°C for 30 min ...
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bioRxiv - Molecular Biology 2021Quote: The VHH-coding regions within parent libraries and 1-round target-enriched sublibraries were PCR amplified and purified using AMPure XP beads (Beckman Coulter, High Wycombe, UK). Then ...
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bioRxiv - Biochemistry 2020Quote: Sedimentation velocity experiments were performed using a ProteomeLab XL-1 analytical ultracentrifuge with the use of an AN60-Ti rotor and Epon-2 channel centerpieces (Beckman Coulter, Brea, CA, USA). Protein samples were diluted with SANS buffer to concentrations yielding an absorbance of 0.5 at 280 nm as measured with an 8454 UV–Vis spectrophotometer (Agilent ...
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bioRxiv - Microbiology 2022Quote: ... Reaction products were verified on a 1% agarose gel and purified using the AMPure XP magnetic Bead protocol (Beckman Coulter Inc., Brea, CA, USA). PCR2 was performed in a 25 μl reaction ...
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bioRxiv - Systems Biology 2020Quote: ... and the resultant supernatant was ultracentrifuged at 100,000xg for 40 minutes at 4°C (rotor TLA 120.2; Beckman Coulter 343778 1 ml uncapped tubes).
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bioRxiv - Physiology 2021Quote: ... sEV were further isolated from lEV-depleted supernatants following a 100,000 xg ultracentrifugation step for 1 hour at 4°C (rotor MLA-50, Beckman Coulter Optima MAX-XP Ultracentrifuge) and two washes in NaCl before resuspension in NaCl.
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bioRxiv - Neuroscience 2023Quote: ... PI (1 μl/100 μl) was added 5 minutes before starting the reading on the flow cytometer CytoFlex S (Beckman Coulter, Brea, CA, USA). Results were shown and analyzed through CytExpert flow cytometry analysis software.
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bioRxiv - Microbiology 2023Quote: The amplicons obtained with each of the strategies (using pools 1 and 2) were pooled in each group and purified with one volume of AMPure XP beads (Beckman Coulter, Brea, CA, USA). The beads were washed with 80% ethanol ...
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bioRxiv - Biophysics 2023Quote: ... Cell debris was removed by centrifugation at 20,000 rpm at 4°C for 1 h (Avanti® J-E centrifuge from Beckman Coulter, rotor: JLA 25.50). The supernatant was collected and loaded onto a pre-equilibrated 5 ml His-Trap FF crude column ...
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bioRxiv - Bioengineering 2023Quote: ... and a humidity of 85 % in 48-well Flowerplates containing 800 µL defined CGXII medium with 0.5 % (w/v) glucose inoculated to an OD600 of 1 by using the BioLector microbioreactor for 24 h (Beckman Coulter Life Sciences, Aachen, Germany). Increasing concentrations of ANT (final concentration of 0 ...
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bioRxiv - Neuroscience 2023Quote: ... The samples were spun for 1 hour at RT in a Beckman ultracentrifuge using SW 120 Ti rotor (Beckman Coulter, Inc., Brea, CA, USA) at 100,000xg ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were washed with 1 ml flow buffer once then resuspended in 200 μl flow buffer and measured using a CytoFLEX cytometer (Beckman Coulter, Jersey City, NJ).
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bioRxiv - Biochemistry 2024Quote: ... The samples were then subjected to high-speed ultracentrifugation (100000 g for 1 h) at 20 using an Optima TL-100 benchtop ultracentrifuge (Beckman Coulter, Indianapolis, IN, USA). AgADFs and α-actin alone were processed identically as a controls ...
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bioRxiv - Biochemistry 2020Quote: ... The dialysed proteins were clarified by centrifugation in an Optima MAX-XP tabletop ultracentrifuge (Beckman Coulter Life Sciences) (90,720 x g ...
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bioRxiv - Cancer Biology 2022Quote: ... proteins enriched by IgG-agarose beads were eluted and processed with Agencourt AMPure-XP magnetic beads (Beckman Coulter) as previously described [40] ...
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bioRxiv - Molecular Biology 2021Quote: ... as determined by flow-cytometry with anti-CD34 antibody (#581-PE; Beckman Coulter ...
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bioRxiv - Microbiology 2021Quote: ... and intracellular staining with the anti-p24Gag KC57-FITC antibody (Beckman Coulter), as described previously (67) ...
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bioRxiv - Cancer Biology 2021Quote: ... antibodies and fluorescence data were acquired by Cytoflex flow cytometer (Beckman Coulter). The percentages of T (CD3+ ...
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bioRxiv - Cell Biology 2021Quote: ... Endogenous PAR1 was labeled with anti-PAR1 WEDE antibody (Beckman Coulter, #IM2584) at 1:500 for 1 h on ice ...
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bioRxiv - Biophysics 2020Quote: ... the cells were pre-incubated with the inhibitory 4b4 antibody (Beckman Coulter) at a concentration of 5 µg/mL for >15 min before the measurements (the treatment has been labelled 4b4 in the figures).
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bioRxiv - Immunology 2023Quote: ... then stained with an anti-p24 KC-57 FITC antibody (Beckman Coulter) according to manufacturer protocol ...
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bioRxiv - Immunology 2023Quote: ... then stained with an anti-p24 KC-57 FITC antibody (Beckman Coulter) according to manufacturer protocol ...
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bioRxiv - Microbiology 2024Quote: ... was then added to the labelling reaction and incubated for additional 30 min at room temperature before labeled-virus purification through ultracentrifugation for 1 hour at 35,000 RPM using a rotor SW40Ti (Beckman Coulter Life Sciences, Brea, CA, USA), at 4 °C in a 6–30% OptiPrep (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... Unbound protein was separated from liposomes by transferring 100μl of this mixture to 7×20mm PC ultracentrifuge tubes (Beckman) and carefully layering an equal volume of 0.75M sucrose buffer followed by 25μl of sucrose-free HK buffer ...
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bioRxiv - Cancer Biology 2020Quote: Cells expressing different fluorescent proteins were harvested from culture and counted using a ViCELL-XR (Beckman Coulter, Indianapolis, IN). Cells were washed 3 times in PBS to remove remaining cytokine ...
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bioRxiv - Systems Biology 2021Quote: ... Cells expressing the DsRed fluorescent protein were FAC sorted with a MoFlo cell sorter (Beckman Coulter GmbH, Krefeld, Germany) to obtain a highly enriched fraction for neuronal cells.
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bioRxiv - Microbiology 2023Quote: ... The denatured protein fraction was then removed via ultracentrifugation at 100,000 g for 20 min at 4°C (Beckman Coulter Optima XE-90 – IVD ultracentrifuge with a 42.2 Ti rotor) ...
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bioRxiv - Cell Biology 2020Quote: ... a compensation matrix was calculated using VersaComp Antibody Capture Kit (Beckman Coulter B22804) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... permeabilized and stained for intracellular p24 using anti-p24-PE antibodies (Beckman Coulter). Data were acquired using a FACSVers flow cytometer (BD Biosciences ...
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bioRxiv - Microbiology 2021Quote: ... we used a combination of two antibodies: p24 KC57-FITC (Beckman Coulter, 6604665) and p24 28B7-APC (MediMabs ...
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bioRxiv - Cell Biology 2021Quote: ... a compensation matrix was calculated using VersaComp Antibody Capture Kit (Beckman Coulter, #B22804) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... and anti-human CD64 antibodies (APC) using a CytoFlex S (Beckman Coulter, USA). All the antibodies were purchased from SinoBiological ...
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bioRxiv - Neuroscience 2022Quote: ... The primary antibodies were anti-CD31 (Réf. IM1431U / Fournisseur Beckman Coulter / Clone 5.6E), anti-CD34 (IM2709U / Beckman Coulter / 581) ...
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bioRxiv - Genetics 2024Quote: ... The panel included antibodies for TCR Vβ8 FITC (clone 56C5.2, Beckman Cat# IM1233), CD3 BV711 (clone SK7 ...
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bioRxiv - Biochemistry 2020Quote: ... Protein expression was continued for 24 h before harvesting cells by centrifugation (4°C, 8000 rpm, 10 min in Beckman Coulter Avanti JXN-26 centrifuge ...
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bioRxiv - Biochemistry 2020Quote: ... Protein expression was continued for 18 h before harvesting cells by centrifugation (4°C, 8000 rpm, 10 min in Beckman Coulter Avanti JXN-26 centrifuge ...
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bioRxiv - Biochemistry 2021Quote: ... 300 μL of metabolite pool was aliquoted per target protein replicate and sealed with aluminum foil seals (Beckman Coulter 538619) to create the metabolite chambers ...