Labshake search
Citations for Beckman :
701 - 750 of 1630 citations for 3 2 5 Dioxoimidazolidin 4 yl propanoic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... supernatants were spun for 70 minutes at 200,000 × g and 4°C in an XPN-80 ultracentrifuge equipped with a Type 50.4 Ti rotor (Beckman Coulter ...
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bioRxiv - Genetics 2021Quote: ... from pooled wells) were fixed using 4% paraformaldehyde (PFA) and analyzed for GFP fluorescence by CytoFLEX (Beckman-Coulter) to determine the percentage of infected cells ...
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bioRxiv - Neuroscience 2023Quote: EVs suspensions were pelleted by centrifugation at 100,000 x g for 70 min at 4°C using a 70.1Ti rotor (Beckman) and resuspended in 20 volumes (w/v ...
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bioRxiv - Neuroscience 2023Quote: ... the supernatant was centrifuged at 43,800 RPM for 75 min at 4°C using a Ti45 rotor (Beckman). The floating lipid layer and supernatant were discarded and the centrifuge tube was washed twice with 6 mL PBS ...
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bioRxiv - Neuroscience 2023Quote: ... Fractions were diluted with 15 mL of cold PBS and centrifuged at 100,000 x g for 70 min at 4°C using a 70.1Ti rotor (Beckman). The supernatants were discarded and the pellets (containing EVs ...
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bioRxiv - Cell Biology 2023Quote: ... samples were centrifuged at 80,000 rpm for 20 min at 4°C in a TLA-100.1 rotor (Beckman). The supernatant was carefully removed ...
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bioRxiv - Cancer Biology 2023Quote: ... Following centrifugation at 50,000 rpm for 16 hours at 4 °C in SW 55 Ti Rotor (Beckman Coulter), fractions (100 μL each ...
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bioRxiv - Biophysics 2023Quote: ... 1 mM DTT) and spun at 32,600 × g for 15 h at 4° in SW32 rotor (Beckman Coulter). Ribosome fractions were pooled as shown in Figure 2D ...
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bioRxiv - Biochemistry 2023Quote: ... samples were centrifuged at 80,000 rpm for 20 min at 4°C in a TLA-100.1 rotor (Beckman). The supernatant was carefully removed ...
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bioRxiv - Microbiology 2023Quote: ... concentrated by ultracentrifugation at 22.000 rpm for 2.5 h at 4°C (Optima™ XPN-80 – Beckman Coulter), redissolved in 100 µL of DMEM and stored in small aliquots at −80 °C ...
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bioRxiv - Microbiology 2023Quote: ... This OMV solution was then pelleted by ultracentrifugation at 100,000 x g for 3h at 4°C (Beckman Coulter Optima L-80 XP Ultracentrifuge ...
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bioRxiv - Molecular Biology 2022Quote: ... Gradients were centrifuged at 35K rpm for 120 min at 4°C in a SW-41Ti rotor (Beckman) with maximum acceleration and no brake using a Beckman Optima L-90 Ultracentrifuge ...
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bioRxiv - Molecular Biology 2022Quote: ... and centrifuged at 100,000g for 70 min at 4°C (Optima XPN-80 Ultracentrifuge, Beckman Coulter, California, USA) in a 45ti fixed angle rotor ...
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bioRxiv - Molecular Biology 2024Quote: ... and centrifuged for 1.5 h at 75,000 rpm at 4°C using a TLA-120.2 rotor (Beckman Coulter). After carefully removing the supernatant ...
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bioRxiv - Biochemistry 2024Quote: ... the purified particles were pelleted down by ultra-centrifugation at 30,000 rpm for 3.5 hours at 4 °C (Beckman Coulter ...
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bioRxiv - Microbiology 2024Quote: ... it was concentrated by ultracentrifugation through OptiPrepTM Density Gradient Medium (D1556) at 154,000 g at 4℃ for 2.5 hours using a SW41Ti rotor (Beckman). The pelleted viral particles were suspended in 100 μL of PBS ...
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bioRxiv - Developmental Biology 2023Quote: ... Peptide supernatant was cleared by ultracentrifugation at 100,000□g for 1 hour at 4□°C (Beckman Coulter, 343775), then vacuum-dried overnight.
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bioRxiv - Cell Biology 2023Quote: ... The fraction containing TpPyShell was further concentrated by centrifugation (4000 g, 15 min, 4°C, SX4400 rotor; Beckman) with Amicon Ultra (M.W.4000 ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were harvested by two rounds of centrifugation (6,000 rpm, 10 min, 4°C, JLA-9.1000 rotor; Beckman), resuspended in Buffer A containing 50 mM Tris-HCl pH8.0 ...
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bioRxiv - Neuroscience 2024Quote: ... the samples were ultracentrifuged (4 °C for 60 min) at 100,000×g (Optima MAX Preparative Ultracentrifuge, Beckman Coulter). Supernatants ...
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bioRxiv - Biophysics 2023Quote: ... the supernatant was layered onto PBS/1.5 M sucrose solution in sample/sucrose 4:1 ratio and ultracentrifuged at 264,000 × g on Optima TLA 100.3 ultracentrifuge (Beckman for 2 h at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... the high-speed centrifugation at 167,000g for 4 hours in an SW32 Ti swinging-bucket rotor (Beckman Coulter) was used to separate and further wash the EV from the concentrate collected in the previous step ...
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Faa1 membrane binding drives positive feedback in autophagosome biogenesis via fatty acid activationbioRxiv - Biochemistry 2023Quote: ... Lysates were cleared by centrifugation (20,000 rpm for 45 min at 4°C in a Beckman SW45Ti rotor). The supernatant was incubated with IgG Sepharose beads (GE Healthcare ...
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bioRxiv - Microbiology 2024Quote: ... The eluent was ultracentrifuged (100,000 ×g, 4°C, 16 h) using Optima L-100 XP Ultracentrifuge (Beckman Coulter) to sediment ribosomes.
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bioRxiv - Microbiology 2022Quote: ... The supernatant fractions were collected and centrifuged at 75,600 x g for 90 min at 4 °C (Beckman JA25.50 rotor ...
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bioRxiv - Molecular Biology 2022Quote: ... The elutions were combined and centrifuged at 186,000 x g at 4 °C for 1.5 h in a (TLA-120.1) rotor (Beckman Coulter). Supernatant was discarded and the pellet was resuspended in the RNC buffer at a concentration of ~200 nM ...
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bioRxiv - Biochemistry 2022Quote: ... Membrane contamination was removed by centrifugation 100,000 x g 1.25 hours 4°C in an NVT65.2 rotor (Beckman Coulter). The clarified supernatant was concentrated to 50 µl by ultrafiltration (Sartorius Vivaspin Turbo ...
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Dynamic states of eIF6 and SDS variants modulate interactions with uL14 of the 60S ribosomal subunitbioRxiv - Biochemistry 2022Quote: ... Gradients were centrifuged at 49,100g for 16 to 17 hrs at 4°C in an SW32Ti rotor (Beckman). Gradients were fractionated using a Brandel (UV ...
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bioRxiv - Molecular Biology 2022Quote: ... and spun at 100,000g for 70 minutes at 4°C (Optima XPN-80 Ultracentrifuge, Beckman Coulter, California, USA) in a 45ti fixed angle rotor ...
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bioRxiv - Neuroscience 2022Quote: ... were then spun at 19,000rpm/90 minutes/4°C using an ultracentrifuge and a SW28 hanging rota (Beckman). All supernatant was removed and each viral pellet was resuspended with 300 µl of 1% bovine serum albumin (Tocris Bioscience ...
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bioRxiv - Cell Biology 2023Quote: ... 10% and 6% and ultracentrifuged at 187,000xg for 1:30h at 4°C in Sw32.1Ti rotor (Beckman Coulter). Afterwards ...
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bioRxiv - Genomics 2023Quote: ... Extracts were then centrifuged at 148,000 g for 2.5 hours at 4°C using the SW 28 Ti rotor (Beckman). Resulting pellet was resuspended overnight at 4°C in 1.5 ml of HBSS ...
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bioRxiv - Bioengineering 2023Quote: ... at 4°C using an Optima Max Ultracentrifuge and a TLA-55 rotor (Beckman Coulter, Brea, CA, USA). The pellet from this supernatant (i.e. ...
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bioRxiv - Cell Biology 2023Quote: ... Mitochondrial membranes were pelleted at 72000 xg for 30 min at 4°C using an optimaTM ultracentrifuge (Beckman) with Beckman rotor (TLA 120) ...
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bioRxiv - Biophysics 2024Quote: ... Lysates were then clarified by centrifugation (100,000 xg, 60 min, 4 °C, JA 30.50 rotor, Avanti JXN-26 centrifuge (Beckman)) before filtering the supernatant through 0.45 μm syringe filters ...
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bioRxiv - Microbiology 2024Quote: ... The cells were collected by centrifugation (8,000 rpm, 10 minutes at 4°C, JLA-10.500 rotor (Beckman Coulter)) ...
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bioRxiv - Cell Biology 2024Quote: ... Following an 18-hr centrifugation at 4°C (44,000 rpm, SW55 rotor, Beckman Optima LE-80 K Ultracentrifuge), 420-μl fractions (1-11 ...
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bioRxiv - Developmental Biology 2024Quote: ... Insoluble material was removed by high speed centrifugation (30,000g, 1 hour, at 4°C, in rotor JA12, Beckman) and by 0.45 µm filtration ...
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bioRxiv - Neuroscience 2024Quote: ... Ultracentrifugation was done at 134,000xg for 4h at 4°C using an SW 41 Ti rotor (Beckman Coulter) in an Optima XE-90 ultracentrifuge (Beckman Coulter) ...
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bioRxiv - Biochemistry 2024Quote: ... microsomes from the IVT reactions were first recovered by centrifugation at 4°C in the TLA55 rotor (Beckman) for 20 min at 55,000 rpm ...
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bioRxiv - Immunology 2024Quote: ... and 20% OptiPrep density gradient at 100,000 × g overnight and at 4 °C (SW 40 Ti, Beckman Coulter). The following day ...
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bioRxiv - Microbiology 2024Quote: ... cultures were harvested by centrifugation for 30 minutes at 5,000 rpm and 4 °C in an Avanti JXN-26 Floor Centrifuge using the JXN 12.500 rotor (Beckman). The resulting pellets were resuspended in 40 mL Lysis buffer (20 mM HEPES pH 7.5 ...
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bioRxiv - Cell Biology 2024Quote: ... The post-nuclear supernatants were centrifuged at 60,000 rpm for 60 min at 4°C with a TLA 120.2 rotor (Beckman) to collect ER-mitochondria-enriched fractions ...
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bioRxiv - Biochemistry 2024Quote: ... and subjected to centrifugation at 35,000 rpm for 2.5 hr at 4 °C using a SW41Ti rotor (Beckman). The polysome profile in sucrose gradients was resolved using a Brandel gradient fractionator ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by ultracentrifugation at 100,000 g for 90 min at 4°C (45Ti or TLA110 rotors, Beckman Coulter). The pellet containing EVs was washed in cold 1x PBS (Gibco ...
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bioRxiv - Biophysics 2024Quote: ... and the lysate was cleared by ultracentrifugation for 30 minutes at 4°C (Ti45 rotor, 37,000rpm, Beckman Coulter). The proteins were purified by affinity chromatography using an ÄKTA Start (GE Healthcare ÄKTA Start) ...
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bioRxiv - Genetics 2020Quote: ... PCR products were pooled by region of interest using 2-3 μL of each reaction and the resulting pools of products were purified using a 1X Ampure XP cleanup (Beckman Coulter). Purified libraries were pooled and submitted for paired-end 150 cycle sequencing on an Illumina MiSeq ...
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bioRxiv - Genomics 2021Quote: ... Cells were washed with 25 mm supplemented HBSS before depleting the sample of unnucleated cells using IOTest 3 Lysing solution (Beckman Coulter) following provider instructions ...
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bioRxiv - Microbiology 2021Quote: ... triplicate spore preparations were made in 3 ml of 20% glycerol and suspended in Isoton II (Beckman Coulter, Brea, CA, USA). The particle volume was then measured for a minimum of 100 000 particles per sample using a Multisizer 4e Coulter counter (Beckman Coulter ...
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bioRxiv - Cell Biology 2020Quote: ... with densities ranging from 1.10 to 1.15 were pooled and washed in PBS (Beckman Ti50, 3 h at 42 000 rpm).