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Citations for Beckman :
701 - 750 of 1481 citations for 2 4 Methyl 5 thiazolyl ethyl decanoate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... The lysate was clarified by centrifugation at 48000×g for 45 min at 4°C using a JA-25.50 rotor (Beckman), and applied on a XK 16/20 chromatography column (Cytiva ...
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bioRxiv - Biochemistry 2022Quote: ... The membrane was solubilized for 3 hours at 4°C before separation by ultracentrifugation at 100,000 g (Ti45, Beckman) for 45 min ...
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bioRxiv - Biochemistry 2022Quote: ... The elutions were combined and centrifuged at 100,000 rpm at 4°C for 40 minutes in a TLA120.2 rotor (Beckman Coulter) before resuspension of the ribosomal pellet in RNC buffer containing 1 μM ternatin-4 ...
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bioRxiv - Developmental Biology 2022Quote: ... Gradients were centrifuged at 35.000 g for 3h at 4°C in an ultracentrifuge using the SW40ti rotor head (Beckman). Fractions of 1 mL were collected from the top at 1 mL/min ...
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Reducing mitochondrial ribosomal gene expression does not alter metabolic health or lifespan in micebioRxiv - Cell Biology 2022Quote: ... and then purified using 30% Percoll gradient ultracentrifugation at 95,000g for 30 minutes at +4°C (Beckman sv41 rotor). Purified mitochondria were treated 1:200 with a protease inhibitor cocktail (Thermo Fisher Scientific HALT PI ...
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bioRxiv - Genomics 2022Quote: ... All samples were acquired on a Beckman Coulter CytoFLEX S with 4 lasers (405nm, 488nm, 561nm, 638nm) (Beckman Coulter), and operated by CytExpert Software v2.3 (Beckman Coulter ...
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bioRxiv - Neuroscience 2022Quote: ... and ultracentrifuged at 25,000 g for 20 min 4°C (or 50,000 g for 30 min 4°C) in a Beckman TLA100.3 rotor (Optima™ TLX Ultracentrifuge, Beckman). The pellet was discarded ...
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bioRxiv - Microbiology 2022Quote: ... The gradient was centrifuged at 4°C and 35,000 rpm for 2.5 h using an SW 40 Ti rotor (Beckman Coulter). Afterward ...
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bioRxiv - Microbiology 2022Quote: ... which was spun in a ultracentrifuge at 30,000 x g for 2h at 4°C (SW 60 Ti rotor, Beckman) The pellet was resuspended in 20 μl of PBS for 1 h at 4 °C ...
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bioRxiv - Neuroscience 2022Quote: ... supernatant was centrifuged for 3 h at 115,000 g and 4°C using the XPN-80 ultracentrifuge equipped with a Ti70 rotor (Beckman). EV pellets were resolved in 1 mL ice-cold PBS (Gibco ...
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bioRxiv - Microbiology 2022Quote: ... Glycoproteins in the supernatants were pelleted by centrifugation at 100,000×g at 4°C for 90 min using a TLA-55 rotor (Beckman). The resultant pellets ...
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bioRxiv - Microbiology 2022Quote: ... The post-nuclear supernatant was adjusted to 0.8 M sucrose and loaded in the middle of a step gradient ranging from 0.1 to 1.8 M sucrose in STKM buffer and centrifuged at 50’000 g for 1 h at 4°C in a TLA-120.1 fixed-angle rotor (Beckman Coulter). LDs formed a white cushion on top of the tube and were collected by aspiration of the upper fraction of the gradient (125 µl).
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bioRxiv - Biochemistry 2023Quote: ... The lysate was clarified by centrifugation (183,960 x g, 88 min, 4°C) in a Type 70Ti rotor (Beckman). The supernatant was mixed with 2 mL of IgG Sepharose 6 Fast Flow beads (Cytiva ...
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bioRxiv - Biochemistry 2023Quote: ... The lysate was clarified via centrifugation (66,000 x g, 30 min, 4°C) in a Type 70 Ti rotor (Beckman). The supernatant was mixed with 1.5 mL of anti-FLAG M2 affinity gel (Sigma-Aldrich ...
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bioRxiv - Immunology 2022Quote: ... then samples were centrifuged at 2,700 RPM for 10 minutes at 4°C in a tabletop centrifuge (Beckman Coulter). The supernatant (serum ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatant was centrifuged at 200,000 x g for 2.5 h (high g) at 4°C in a TLA-100.3 fixed angle rotor (k-factor 14) (Beckman Coulter) and the pellet was resuspended in 25 ∝l lysis buffer ...
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bioRxiv - Cell Biology 2023Quote: ... Gradients were spun at 45,000 RPM for 16 hours at 4°C in SW55Ti rotor using an ultracentrifuge (Beckman). After centrifugation ...
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Comparative membrane proteomics reveals diverse cell regulators concentrated at the nuclear envelopebioRxiv - Cell Biology 2023Quote: ... The gradients then were centrifuged at 35,000 rpm (210,000g) for 1 h at 4°C with no brake in an SW41Ti rotor (Beckman Coulter). Two samples were collected from the nucleus gradient ...
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bioRxiv - Biochemistry 2023Quote: ... The lysate was then clarified (using a JA 25.50 Beckman rotor at 40000x g for 30 min, 4°C) and the soluble fraction was incubated with 2 ml of Glutathione Sepharose 4B resin for 2h at 4°C under constant agitation ...
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bioRxiv - Cancer Biology 2023Quote: ... 100 μg/mL cycloheximide if reaction contained cycloheximide) for 3.5 hrs at 240,000 g at 4 °C using a SW41 rotor (Beckman Coulter). Gradients were fractionated using Teledyne Isco Tris Peristaltic Pump and fractions were collected and pooled according to the UV trace ...
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bioRxiv - Biochemistry 2023Quote: ... Density gradient centrifugation was done at 50,000 rpm for two hours at 4 °C using a 70.1 Ti rotor (Beckman Coulter). Post centrifugation ...
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bioRxiv - Neuroscience 2023Quote: ... the supernatant was collected and applied for further centrifugation at 40,000 rpm for 1 h at 4°C (45 Ti fixed-angle rotor, Beckman) to pellet the membranes ...
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bioRxiv - Immunology 2023Quote: ... and then centrifuged at 260,800 x g for 2.5 h at 4°C in an Optima L-100XP ultracentrifuge (Beckman–Coulter) using the SW41Ti rotor with brake ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatant was centrifuged at 200,000 x g for 2.5 h (high g) at 4°C in a TLA-100.3 fixed angle rotor (k-factor 14) (Beckman Coulter) and Red-C12 fluorescence of the starting material ...
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bioRxiv - Cell Biology 2024Quote: ... The lysate was clarified by centrifugation (66,000 x g, 30 minutes, 4 °C) in a Type 70Ti rotor (Beckman). For His-ZZ-TEV-Halo-CCSer2650–850 ...
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bioRxiv - Cell Biology 2024Quote: ... 0.1 mM PMSF buffer and subjected to ultracentrifugation at 36,000 rpm at 4°C for 2h using a SW41 rotor (Beckman) and 14 x 89 mm polypropylene tubes (Beckman ...
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bioRxiv - Cell Biology 2024Quote: ... Solutions of 15% and 23% Optiprep were layered on top or below the sample and centrifuged at 4°C for 16 h at 120,000g in a SW 28 Ti swinging bucket rotor (Beckman).
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bioRxiv - Cell Biology 2024Quote: ... The gradients were centrifuged at 39,000 rpm for 2.5 h at 4°C using a SW41Ti rotor (Beckman Instruments), and 21 fractions of 0.5 ml were collected ...
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bioRxiv - Neuroscience 2024Quote: ... The supernatant was passed through a 0.22 μm filter before undergoing ultracentrifugation at 100,000g for 70 min at 4°C (Optima-XE SW41 Beckman Coulter). The resulting pellet was then resuspended in 2 ml of 0.475 M sucrose solution prepared in double-filtered PBS ...
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bioRxiv - Microbiology 2024Quote: ... Phage particles were pelleted by centrifugation at 75,000 x g at 4°C for 1 h using a 50.2 Ti rotor (Beckman Coulter) and resuspended in the phage buffer.
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bioRxiv - Microbiology 2024Quote: ... the supernatants were loaded onto 1 mL of 30% sucrose in PBS buffer and centrifuged at 25,000 rpm at 4°C for 3 hours using the 90AT rotor (Beckman). The pelleted viral particles were resuspended in 100 μL of PBS and fixed with formaldehyde (working concentration 0.1% ...
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bioRxiv - Microbiology 2024Quote: ... were centrifuged at approximately 108,000 x g for 107 min at 4°C using an ultracentrifuge device equipped with a TLA 100.3 fixed-angle rotor (Beckman). The supernatants were retained as soluble fractions and kept at 4°C for downstream analyses ...
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bioRxiv - Immunology 2024Quote: ... Sartorus) at 100,000 x g for 70 min at 4°C using the ultracentrifuge Optima MAX-TL (Beckman Coulter). The pellets were suspended in PBS(– ...
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bioRxiv - Cell Biology 2024Quote: ... The lysate was clarified via centrifugation (66,000 x g, 30 minutes, 4 °C) in a Type 70 Ti rotor (Beckman). The supernatant was mixed with 1.5 mL of α-FLAG M2 affinity gel (Sigma–Aldrich ...
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bioRxiv - Cell Biology 2024Quote: ... The lysate was clarified by centrifugation (183,960 x g, 88 minutes, 4 °C) in a Type 70Ti rotor (Beckman). The supernatant was mixed with 2 mL of IgG Sepharose 6 Fast Flow beads (Cytiva ...
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bioRxiv - Plant Biology 2024Quote: ... the supernatant after the 40,000 g spin was transferred to ultracentrifuge tubes and centrifuged at 100,000 g for 1 h at 4 °C (TLA100.3 rotor, Optima TLX Ultracentrifuge; Beckman Coulter). P40 and P100-P40 pellets were resuspended in cold and filtered VIB or 100 mM Tris pH 7.4 and either used immediately or stored at –80 °C until further use.
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bioRxiv - Plant Biology 2024Quote: ... tubes and centrifuged at 40,000 g for 1 h at 4 °C (TLA100.3 rotor, Optima TLX Ultracentrifuge; Beckman Coulter). Where indicated ...
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bioRxiv - Biochemistry 2024Quote: ... The unbroken cells and debris were removed by centrifugation at 20,000 x g for 10 min at 4 °C (Beckman Coulter Avanti J20 XPI centrifuge ...
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bioRxiv - Biochemistry 2024Quote: ... dimethylammonio)-1-propanesulfonate)] and lysates were cleared by centrifugation at 75,600 g at 4 °C for 30 min using a JA 25.50 rotor (Beckman-Coulter). Next ...
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bioRxiv - Neuroscience 2024Quote: ... EV-depleted FBS medium was obtained by the ultracentrifugation of BV-2 complete medium supplemented with 20% FBS for 18h at 100.000 × g and 4°C in 38.5 mL polyallomer Ultra Clear ultracentrifuge tubes (Beckman Coulter), using an Optima XPN-80 ultracentrifuge and an SW 32 Ti Rotor (Beckman Coulter ...
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bioRxiv - Molecular Biology 2024Quote: ... The samples were ultracentrifuged at 100,000 × g for 135 min at 4°C (MLA-55 rotor, Beckman Coulter, USA). After removal of the supernatant ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... containing cell nucleus and other heavy organelles was discarded and supernatant was centrifuged for 30 min at 48,000 × g at 4 °C (Beckman Avanti J-251 Ultra-centrifuge ...
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bioRxiv - Neuroscience 2024Quote: ... Tubes were centrifugated at 100,000 x g (32,500 RPM) for 3 hours at 4°C using a SW55Ti rotor (Beckman Coulter). Synaptosomes enriched in the interface between 1.0-1.25 M sucrose were collected and diluted with ice-cold 0.1 mM CaCl2 ...
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bioRxiv - Cell Biology 2024Quote: ... and the membranes were pelleted at 100,000 g for one hour at 4°C in a TLA 100.3 rotor (Beckman-Coulter). Afterwards the supernatant was taken off and stored at −20°C (“S100” ...
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Syntaxin-2 inhibits excessive phagocytic uptake but promotes phagolysosomal clearance in macrophagesbioRxiv - Cell Biology 2024Quote: ... The samples were then centrifuged at 100,000 g for 1 hr at 4°C using a SW40Ti rotor (Beckman). Phagosomes were recovered from the interface of 10 and 25% sucrose (Fig ...
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bioRxiv - Biophysics 2024Quote: ... Lysates were clarified by ultracentrifugation at 34,000 rpm for 45 minutes at 4°C in a Type 45.1 Ti rotor (Beckman-Coulter). The supernatants were then passed through a 0.22 μm syringe sterile filter (Thermo-Fisher ...
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bioRxiv - Neuroscience 2024Quote: ... The homogenate was layered on the gradient and centrifuged (3h, 4°C, 160,000 xg) using a SW40 rotor (Beckman). Top layer of debris was removed and ∼18 fractions of 500 µl were collected from each gradient from top (fraction 1 ...
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bioRxiv - Biophysics 2024Quote: Tau proteins were diluted in cold 1x BRB80 to a concentration of a few tenths of micromolar and centrifuged to remove aggregates (10 min/4°C /215,000 x g in Type 70 Ti rotor [Beckman]). Prior to use ...
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bioRxiv - Biophysics 2024Quote: ... The lysate was clarified via centrifugation (66,000 x g, 30 min, 4°C) in a Type 70 Ti rotor (Beckman), before being incubated overnight at 4°C with 1.5 mL of anti-FLAG M2 affinity gel (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2024Quote: ... The crude membrane fraction containing MsbA was isolated by ultracentrifugation (100000g, 45 min, 4°C, Beckman Coulter rotor Ti70). About 2 mg of MsbA-enriched membranes were solubilized with 1% DDM (w/v ...