Labshake search
Citations for Beckman :
651 - 700 of 761 citations for Two Hybrid System Construction kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... 50μl of heparin-blood was used for trucount analysis with the non-centrifugation PerFix-NC kit (Beckman Coulter). Cells were first stained extracellular with α-CD4-APC (02 ...
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bioRxiv - Developmental Biology 2020Quote: ... GEMs were harvested and the cDNAs were amplified and cleaned up with SPRIselect Reagent Kit (Beckman Coulter, B23318). Indexed sequencing libraries were constructed using Chromium Single Cell 3’ Library v2 (10X Genomics ...
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bioRxiv - Immunology 2021Quote: ... Stimulated cells were fixed and permeabilized with the Foxp3 staining kit (eBioscience) or IntraPrep Permeabilizaton Reagent (Beckman Coulter) and then stained with combinations of Foxp3 (FJK-16s ...
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bioRxiv - Cancer Biology 2020Quote: ... cDNA was amplified by PCR and the cDNA product was purified with the SPRIselect Reagent Kit (Beckman Coulter). Sequencing libraries were constructed using the reagents provided in the Chromium Single-Cell 3’ Library Kit following the user guide ...
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bioRxiv - Cell Biology 2022Quote: Total RNA of Caco-2 cells and human ileum organoids was extracted using RNAdvance Tissue kit (Beckman Coulter) and total RNA of mouse organoids was isolated using Quick-RNA Miniprep Plus Kit (Zymo Research) ...
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bioRxiv - Immunology 2022Quote: ... The occult blood in mouse fecal samples was detected using Hemoccult Test Kit (Beckman Coulter Inc, Fullerton, CA).
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bioRxiv - Genomics 2023Quote: ... The fragmented aRNA was size-selected with 0.8x AMPure RNA beads (RNAClean XP Kit, Beckman Coulter, Cat. # A63987) and eluted in 18 μL nuclease-free water ...
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bioRxiv - Neuroscience 2023Quote: RNA was extracted from wild-type mouse livers using the Agencourt RNAdvance Tissue kit (Beckman Coulter, Brea, CA), and cDNA was synthesized using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems ...
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bioRxiv - Genomics 2024Quote: ... Samples too diluted to be compatible with the kit were first concentrated using AMPure Beads (Beckman Coulter, #A63880) and re-quantified ...
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bioRxiv - Molecular Biology 2024Quote: ... DNA was isolated from formalin-fixed paraffin embedded (FFPE) tissue sections using a FormaPure kit (Beckman Coulter, C16675). ddPCR with probes to detect the GNAQ R183Q mutation (Table S1 ...
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Definition and Signatures of Lung Fibroblast Populations in Development and Fibrosis in Mice and MenbioRxiv - Cell Biology 2020Quote: ... GEMs were collected and the cDNA was amplified and purified with SPRIselect Reagent Kit (Beckman Coulter, Brea, CA, USA). Indexed sequencing libraries were constructed using Chromium Single-Cell 3′ Library Kit for enzymatic fragmentation ...
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bioRxiv - Microbiology 2021Quote: ... Products of PCR1 and PCR2 were purified using AMPure XP PCR product cleanup and size selection kit (Beckman Coulter), following the manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: ... PCR products were purified using the AMPure XP solid-phase reversible immobilization kit (SPRI; Beckman Coulter, cat. no. A63881) and subjected to Illumina next-generation sequencing ...
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bioRxiv - Neuroscience 2020Quote: ... 17β-estradiol levels were determined in plasma using a commercially available radioimmunoassay kit (ultrasensitive estradiol RIA, Beckman Coulter, DSL4800). All samples were run in duplicate ...
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bioRxiv - Genomics 2021Quote: ... Library cleanup was done using Zymo Research DNA Clean & Concentrator kit followed by AMPure XP bead (Beckman Coulter #A63880) size selection ...
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bioRxiv - Immunology 2021Quote: ... Colon length was measured and blood in the stool was assessed using a hemoccult blood test kit (Beckman Coulter). Colon sections were fixed in 10% phosphate buffered formalin ...
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bioRxiv - Cell Biology 2020Quote: ... GEMs were broken and the single strand cDNA cleaned up with DynaBeads (Fisher) and SPRIselect Reagent Kit (Beckman Coulter). cDNA was amplified ...
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bioRxiv - Immunology 2022Quote: The TCR V beta repertoire kit contained antibodies to over 76% of V βeta families (Beckman Coulter, Fullerton, CA). The cells were stained with these antibodies in combination with tetramers or dextramers for 20 minutes at RT to determine the TRBV repertoire of antigen specific cells ...
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bioRxiv - Cancer Biology 2023Quote: ... ligated with index adaptors and size selected with cleanups between each step using the SPRIselect Reagent kit (Beckman Coulter). Quality control and quantification of the resulting dual-indexed barcoded libraries was performed with Agilent TapeStation and by qPCR (NEBNext Library Quant Kit for Illumina ...
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bioRxiv - Evolutionary Biology 2023Quote: ... amplicons were purified after each PCR step using the Agencourt AMPure XP purification kit (Beckman Coulter Inc., Brea, USA), and amplicon fragment size and quantification were checked using a Bioanalyzer (Agilent ...
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bioRxiv - Microbiology 2023Quote: ... The libraries construction was carried out with Agilent’s SureSelect Strand-Specific RNA Library Preparation Kit followed by size selection with AMPure XP beads (Beckman Coulter ...
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bioRxiv - Immunology 2023Quote: ... Preamplification was then performed on the products which were subsequently purified using the SPRIselect Reagent Kit (Beckman Coulter, B23318). The paired V(D)J BCR sequences were enriched with 10X BCR primers ...
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bioRxiv - Microbiology 2024Quote: Total RNA was extracted from persistently BoDV-2-infected Vero cells using Agencourt® RNAdvance Tissue kit (Beckman Coulter) and the KingFisher Flex system (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... Serum 17β-estradiol levels in female rats and serum testosterone levels in male rats were assayed using commercially available radioimmunoassay (RIA) kits from Beckman Coulter (Brea ...
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bioRxiv - Genomics 2020Quote: Pieces of caudal fin sampled from those 1,152 fish were sent to Gentyane genotyping platform (INRAE, Clermont-Ferrand, France) for DNA extraction using the DNAdavance kit from Beckman Coulter following manufacturer instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... isotype controls for intracellular stains and subjected to post-acquisition compensation using VersaComp Antibody Capture Bead Kit (Beckman Coulter #B22804). Data was analyzed using Kaluza Software (Beckman Coulter) ...
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bioRxiv - Plant Biology 2021Quote: ... The pool was then purified using the solid phase reversible immobilization (SPRI) Agencourt AMPure XP kit (Beckman Coulter, Milan, Italy).
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bioRxiv - Microbiology 2020Quote: ... and the resulting cDNA was PCR amplified (11 cycles) and purified using the Agencourt AMPure XP kit (Beckman Coulter Genomics). For Illumina NextSeq sequencing ...
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bioRxiv - Microbiology 2021Quote: Total RNA was isolated from Vero E6 and Caco-2 cells 48 hours after infection using RNAdvance Viral Kit (Beckman) using a KingFisher and RNeasy Mini Kit (Qiagen) ...
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bioRxiv - Developmental Biology 2022Quote: ... TH concentrations are measured by a medical laboratory in Perpignan (Médipole) using an ELISA kit (Access Free T3, T4, Beckman Coulter).
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bioRxiv - Plant Biology 2019Quote: ... were combined in a barcode-wise manner and purified using the Agencourt AMPure XP kit (Beckman Coulter, Brea, United States) with a ratio of 0.7 mL AMPure XP beads per 1 mL of sample ...
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bioRxiv - Physiology 2019Quote: ... Agencourt AMPure XP PCR purification kit (5 ml Beckman Coulter Part No. A63880; 60 ml Beckman Coulter Part No. A63881) was used ...
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bioRxiv - Immunology 2021Quote: ... The first-strand synthesis reaction was purified using the Ampure Size Select Magnetic Bead Kit at a ratio of 0.6X (Beckman Coulter). Following ...
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bioRxiv - Immunology 2021Quote: ... The first PCR reaction was purified using the AMPure Size Select Magnetic Bead Kit at a ratio of 0.6X (Beckman Coulter). Amplicon libraries were then prepared according to the Pacific Biosciences Multiplex SMRT Sequencing protocol and sequenced on a Pacific Biosciences Sequel platform (Pacific Biosciences ...
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bioRxiv - Genomics 2022Quote: Pieces of caudal fin sampled from 463 fish (288 from LB line and 175 from LC line) were sent to Gentyane genotyping platform (INRAE, Clermont-Ferrand, France) for DNA extraction using the DNAdvance kit from Beckman Coulter following manufacturer instructions and genotyping using the newly constructed HD SNP array.
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bioRxiv - Molecular Biology 2024Quote: ... Libraries were prepared using 2S Plus DNA Library Kit adapters (Swift Biosciences) and size-selected using SPRIselect beads (Beckman Coulter) before being amplified and sent for paired-end sequencing on the NovaSeq 6000 (150 bp reads).
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bioRxiv - Molecular Biology 2024Quote: ... The initial PCR products of rbcL F3R3 were purified using an Agencourt AMPure XP kit (Beckman Coulter, Fullerton, CA, USA). To construct the DNA libraries for the second PCR ...
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bioRxiv - Pathology 2024Quote: RNA from rat liver tissue was snap frozen in RNAlater and lysed using lysis buffer (RNAdvanced Kit, A32646, Beckman Coulter), 20µL Proteinase K on a TissueLyser II (Qiagen ...
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bioRxiv - Genetics 2023Quote: ... clean-up and flowcell loading following the protocol for SQK-LSK112 sequencing kit (Version GDE_9141_v112_revC_01Dec2021) with the following modifications: Incubation with and elution from AMPure XP beads (Beckman Coulter, Cat. No. A63882) was extended to 30 minutes each ...
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bioRxiv - Microbiology 2023Quote: ... The rest of the DNA purification process was performed using a GenFind v2 DNA extraction kit (Beckman Coulter, Indianapolis, U.S.) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... The rest of the DNA purification process was performed using a GenFind v2 DNA extraction kit (Beckman Coulter, Indianapolis, U.S.) following the manufacturer’s protocol ...
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bioRxiv - Genetics 2023Quote: ... PCR products were purified with the Qiagen MinElute PCR Purification Kit and AMPure XP beads (Beckman Coulter, Cat. No. A63880) and resuspended in ultrapure nuclease-free distilled water ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The upper aqueous phase was further processed for total RNA extraction using the Agencourt RNAdvance Tissue Kit (Beckman Coulter, Germany) on a KingFisher Flex Purification System (Thermo Fisher Scientific ...
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bioRxiv - Evolutionary Biology 2023Quote: We extracted DNA from fin clips collected from wild-caught fish using the Agencourt DNAdvance kit (Beckman Coulter, Brea, California). We used half-reactions but otherwise followed the manufacturer’s instructions for DNA extraction ...
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bioRxiv - Microbiology 2023Quote: Subsequent second-strand synthesis was performed using the Sequenase 2.0 kit (Thermo #70775Z1000UN) and amplicons cleaned with 1.8 × AMPure XP magnetic beads (Beckman Coulter #A63882). Purified cDNA samples were stored at −20°C until library preparation.
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bioRxiv - Microbiology 2019Quote: ... The first and second PCR reaction products were purified using AMPure XP PCR product cleanup and size selection kit (Beckman Coulter), following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2019Quote: ... a purification step was performed using MiniELute Qiagen kit and size separation on Agencourt AMPure XP beads (A63882, Beckman Coulter, Inc) to remove PCR Nextera primers.
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bioRxiv - Genetics 2019Quote: ... Three PCR reactions were pooled and the PCR product was purified using the Agencourt AMPure XP PCR purification kit (Beckman Coulter) and sequenced using MiSeq (Illumina).
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bioRxiv - Cancer Biology 2020Quote: ... Amplified libraries were purified with the Qiagen PCR clean-up MinElute kit and size selected for fragments less than 600 bp using the Agencourt AMPure XP beads (Beckman Coulter). Libraries were quality controlled by Qubit and Agilent DNA Bioanalyzer analysis ...
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bioRxiv - Developmental Biology 2021Quote: ... cDNA was prepared using Takara Clontech SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Cat.No.:634890) using 8 cycles of amplification and purified using Agencourt AMPure XP beads (Beckman Coulter, A63880). 1 ng of each sample cDNA was used for library preparation ...